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    Charles R Kiefer PhD

    TitleAssociate Professor
    InstitutionUniversity of Massachusetts Medical School
    DepartmentEmergency Medicine
    AddressUMass Memorial Health Care Inc.
    365 Plantation Street, Biotech One
    Worcester MA 01605
    Phone774-442-9629
      Other Positions
      InstitutionUMMS - School of Medicine
      DepartmentPathology
      DivisionAcademic Pathology

        Overview 
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        Academic Background

        PhD, 1981, Medical College of Georgia

        Charles Kiefer

        Fractional C-Reactive Protein: biomarker of internal acute tissue damage 

         

        Since our discovery and publication in 2005 of the close temporal correlation of fractional forms of C-Reactive Protein (fracCRP) with internal acute tissue damage, our laboratory has been involved in the characterization of fracCRP and its measurement in those arriving at the Emergency Department of UMass Memorial with chest pain, shortness of breath, or analogous symptoms of Acute Coronary Syndrome (ACS).

         

        The basic discovery was first translated into a method for chemical capture of fracCRP and its measurement by size exclusion HPLC, validated prospectively with post-troponin test/first-draw plasma specimens from patients arriving with symptoms of ACS, and retrospectively with post-troponin test/first-draw plasma or serum specimens from patients with ACS as the discharge diagnosis (2012).  Overlapping with the first study, we developed a chemiluminescent immunoassay for fracCRP with monoclonal antibodies that have been designed to recognize an epitope accessible on the fractional (linear) forms of circulating CRP but sterically inaccessible on the native CRP pentameric disc.  More recently, we have been engaged in the development of a semi-quantitative lateral flow immunoassay for fracCRP (using the same monoclonal antibodies) that is designed to accelerate the triage of emergency patients, within the context of a workup for ACS, by providing for a cutoff result with 100% specificity.



        Bibliographic 
        selected publications
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        1. Kiefer CR, Stock RE, Flanagan SS, Darling CE, Smith CS, Snyder LM. Early verification of myocardial ischemia with a novel biomarker of acute tissue damage: C-reactive protein fractional forms. Clin Chim Acta. 2012 Oct 9; 413(19-20):1536-41.
          View in: PubMed
        2. Kiefer CR, McKenney JB, Trainor JF, Snyder LM. Pulse-pressure-driven neutral lipid accumulation and correlative proinflammatory markers of accelerated atherogenesis. Atherosclerosis. 2005 Nov; 183(1):17-24.
          View in: PubMed
        3. Kiefer CR, McKenney JB, Trainor JF, Snyder LM. Maturation-dependent acquired coronary structural alterations and atherogenesis in the Dahl sodium-sensitive hypertensive rat. Circulation. 2002 Nov 5; 106(19):2486-90.
          View in: PubMed
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