Charles R Kiefer PhD
|Institution||University of Massachusetts Medical School|
|Address||UMass Memorial Health Care Inc.|
365 Plantation Street, Biotech One
Worcester MA 01605
|Institution||UMMS - School of Medicine|
PhD, 1981, Medical College of Georgia
Fractional C-Reactive Protein: biomarker of internal acute tissue damage
Since our discovery and publication in 2005 of the close temporal correlation of fractional forms of C-Reactive Protein (fracCRP) with internal acute tissue damage, our laboratory has been involved in the characterization of fracCRP and its measurement in those arriving at the Emergency Department of UMass Memorial with chest pain, shortness of breath, or analogous symptoms of Acute Coronary Syndrome (ACS).
The basic discovery was first translated into a method for chemical capture of fracCRP and its measurement by size exclusion HPLC, validated prospectively with post-troponin test/first-draw plasma specimens from patients arriving with symptoms of ACS, and retrospectively with post-troponin test/first-draw plasma or serum specimens from patients with ACS as the discharge diagnosis (2012). Overlapping with the first study, we developed a chemiluminescent immunoassay for fracCRP with monoclonal antibodies that have been designed to recognize an epitope accessible on the fractional (linear) forms of circulating CRP but sterically inaccessible on the native CRP pentameric disc. More recently, we have been engaged in the development of a semi-quantitative lateral flow immunoassay for fracCRP (using the same monoclonal antibodies) that is designed to accelerate the triage of emergency patients, within the context of a workup for ACS, by providing for a cutoff result with 100% specificity.
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