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    Richard A Tuft PhD

    TitleResearch Associate Professor
    InstitutionUniversity of Massachusetts Medical School
    DepartmentMicrobiology and Physiological Systems
    AddressUniversity of Massachusetts Medical School
    373 Plantation Street
    Worcester MA 01605
    Phone508-856-2383
      Other Positions
      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentBiochemistry and Molecular Pharmacology

        Overview 
        Narrative

        Academic Background

        1963, B.S., Pennsylvania State University
        1966, M.S.E.E., Massachusetts Institute of Technology
        1972, Ph.D., Worcester Polytechnic Institute

        Development of New Optical/Electronic Microscopes

        Photo: Richard A. TuftMy current research interest is the development of newoptical/electronic microscopes, and their application to the problems ofimaging rapid changes in the concentration of intracellular ions andmolecules. A microscope incorporating fast piezoelectric focusing (at 1micrometer per millisecond), a low-noise high-quantum-efficiency solidstate CCD camera, and ultraviolet and visible laser illumination iscurrently being used to study intracellular [Ca2+] near the plasma membrane of smooth muscle cells,the role of Ca2+ and other secondmessengers in the chemotaxis of white blood cells, and [Ca2+] dynamics in secretory cells.

        Figure

        Research Figure



        Bibliographic 
        selected publications
        List All   |   Timeline
        1. Lefkowitz JJ, Fogarty KE, Lifshitz LM, Bellve KD, Tuft RA, ZhuGe R, Walsh JV, De Crescenzo V. Suppression of Ca2+ syntillas increases spontaneous exocytosis in mouse adrenal chromaffin cells. J Gen Physiol. 2009 Oct; 134(4):267-80.
          View in: PubMed
        2. Li X, Standley C, Sapp E, Valencia A, Qin ZH, Kegel KB, Yoder J, Comer-Tierney LA, Esteves M, Chase K, Alexander J, Masso N, Sobin L, Bellve K, Tuft R, Lifshitz L, Fogarty K, Aronin N, DiFiglia M. Mutant huntingtin impairs vesicle formation from recycling endosomes by interfering with Rab11 activity. Mol Cell Biol. 2009 Nov; 29(22):6106-16.
          View in: PubMed
        3. Bao R, Lifshitz LM, Tuft RA, Bellvé K, Fogarty KE, ZhuGe R. A close association of RyRs with highly dense clusters of Ca2+-activated Cl- channels underlies the activation of STICs by Ca2+ sparks in mouse airway smooth muscle. J Gen Physiol. 2008 Jul; 132(1):145-60.
          View in: PubMed
        4. Zhang YA, Tuft RA, Lifshitz LM, Fogarty KE, Singer JJ, Zou H. Caffeine-activated large-conductance plasma membrane cation channels in cardiac myocytes: characteristics and significance. Am J Physiol Heart Circ Physiol. 2007 Oct; 293(4):H2448-61.
          View in: PubMed
        5. Ritland Politz JC, Tuft RA, Pederson T. Photoactivation-based labeling and in vivo tracking of RNA molecules in the nucleus. CSH Protoc. 2006; 2006(6).
          View in: PubMed
        6. De Crescenzo V, Fogarty KE, Zhuge R, Tuft RA, Lifshitz LM, Carmichael J, Bellvé KD, Baker SP, Zissimopoulos S, Lai FA, Lemos JR, Walsh JV. Dihydropyridine receptors and type 1 ryanodine receptors constitute the molecular machinery for voltage-induced Ca2+ release in nerve terminals. J Neurosci. 2006 Jul 19; 26(29):7565-74.
          View in: PubMed
        7. Chau MD, Tuft R, Fogarty K, Bao ZZ. Notch signaling plays a key role in cardiac cell differentiation. Mech Dev. 2006 Aug; 123(8):626-40.
          View in: PubMed
        8. Follit JA, Tuft RA, Fogarty KE, Pazour GJ. The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly. Mol Biol Cell. 2006 Sep; 17(9):3781-92.
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        9. Bellve KD, Leonard D, Standley C, Lifshitz LM, Tuft RA, Hayakawa A, Corvera S, Fogarty KE. Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells. J Biol Chem. 2006 Jun 9; 281(23):16139-46.
          View in: PubMed
        10. ZhuGe R, DeCrescenzo V, Sorrentino V, Lai FA, Tuft RA, Lifshitz LM, Lemos JR, Smith C, Fogarty KE, Walsh JV. Syntillas release Ca2+ at a site different from the microdomain where exocytosis occurs in mouse chromaffin cells. Biophys J. 2006 Mar 15; 90(6):2027-37.
          View in: PubMed
        11. Politz JC, Tuft RA, Prasanth KV, Baudendistel N, Fogarty KE, Lifshitz LM, Langowski J, Spector DL, Pederson T. Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm. Mol Biol Cell. 2006 Mar; 17(3):1239-49.
          View in: PubMed
        12. Huang S, Lifshitz L, Patki-Kamath V, Tuft R, Fogarty K, Czech MP. Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes. Mol Cell Biol. 2004 Oct; 24(20):9102-23.
          View in: PubMed
        13. Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. Using total fluorescence increase (signal mass) to determine the Ca2+ current underlying localized Ca2+ events. J Gen Physiol. 2004 Sep; 124(3):259-72.
          View in: PubMed
        14. Zhuge R, Fogarty KE, Baker SP, McCarron JG, Tuft RA, Lifshitz LM, Walsh JV. Ca(2+) spark sites in smooth muscle cells are numerous and differ in number of ryanodine receptors, large-conductance K(+) channels, and coupling ratio between them. Am J Physiol Cell Physiol. 2004 Dec; 287(6):C1577-88.
          View in: PubMed
        15. Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. Imaging calcium entering the cytosol through a single opening of plasma membrane ion channels: SCCaFTs--fundamental calcium events. Cell Calcium. 2004 Jun; 35(6):523-33.
          View in: PubMed
        16. De Crescenzo V, ZhuGe R, Velázquez-Marrero C, Lifshitz LM, Custer E, Carmichael J, Lai FA, Tuft RA, Fogarty KE, Lemos JR, Walsh JV. Ca2+ syntillas, miniature Ca2+ release events in terminals of hypothalamic neurons, are increased in frequency by depolarization in the absence of Ca2+ influx. J Neurosci. 2004 Feb 4; 24(5):1226-35.
          View in: PubMed
        17. O'Reilly CM, Fogarty KE, Drummond RM, Tuft RA, Walsh JV. Spontaneous mitochondrial depolarizations are independent of SR Ca2+ release. Am J Physiol Cell Physiol. 2004 May; 286(5):C1139-51.
          View in: PubMed
        18. O'Reilly CM, Fogarty KE, Drummond RM, Tuft RA, Walsh JV. Quantitative analysis of spontaneous mitochondrial depolarizations. Biophys J. 2003 Nov; 85(5):3350-7.
          View in: PubMed
        19. Hayakawa A, Hayes SJ, Lawe DC, Sudharshan E, Tuft R, Fogarty K, Lambright D, Corvera S. Structural basis for endosomal targeting by FYVE domains. J Biol Chem. 2004 Feb 13; 279(7):5958-66.
          View in: PubMed
        20. Politz JC, Tuft RA, Pederson T. Diffusion-based transport of nascent ribosomes in the nucleus. Mol Biol Cell. 2003 Dec; 14(12):4805-12.
          View in: PubMed
        21. Lawe DC, Sitouah N, Hayes S, Chawla A, Virbasius JV, Tuft R, Fogarty K, Lifshitz L, Lambright D, Corvera S. Essential role of Ca2+/calmodulin in Early Endosome Antigen-1 localization. Mol Biol Cell. 2003 Jul; 14(7):2935-45.
          View in: PubMed
        22. Rapizzi E, Pinton P, Szabadkai G, Wieckowski MR, Vandecasteele G, Baird G, Tuft RA, Fogarty KE, Rizzuto R. Recombinant expression of the voltage-dependent anion channel enhances the transfer of Ca2+ microdomains to mitochondria. J Cell Biol. 2002 Nov 25; 159(4):613-24.
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        23. Takizawa N, Schmidt DJ, Mabuchi K, Villa-Moruzzi E, Tuft RA, Ikebe M. M20, the small subunit of PP1M, binds to microtubules. Am J Physiol Cell Physiol. 2003 Feb; 284(2):C250-62.
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        24. Komatsu S, Miyazaki K, Tuft RA, Ikebe M. Translocation of telokin by cGMP signaling in smooth muscle cells. Am J Physiol Cell Physiol. 2002 Sep; 283(3):C752-61.
          View in: PubMed
        25. Zhuge R, Fogarty KE, Tuft RA, Walsh JV. Spontaneous transient outward currents arise from microdomains where BK channels are exposed to a mean Ca(2+) concentration on the order of 10 microM during a Ca(2+) spark. J Gen Physiol. 2002 Jul; 120(1):15-27.
          View in: PubMed
        26. Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. Visualization of Ca2+ entry through single stretch-activated cation channels. Proc Natl Acad Sci U S A. 2002 Apr 30; 99(9):6404-9.
          View in: PubMed
        27. Miyazaki K, Yano T, Schmidt DJ, Tokui T, Shibata M, Lifshitz LM, Kimura S, Tuft RA, Ikebe M. Rho-dependent agonist-induced spatio-temporal change in myosin phosphorylation in smooth muscle cells. J Biol Chem. 2002 Jan 4; 277(1):725-34.
          View in: PubMed
        28. Lawe DC, Chawla A, Merithew E, Dumas J, Carrington W, Fogarty K, Lifshitz L, Tuft R, Lambright D, Corvera S. Sequential roles for phosphatidylinositol 3-phosphate and Rab5 in tethering and fusion of early endosomes via their interaction with EEA1. J Biol Chem. 2002 Mar 8; 277(10):8611-7.
          View in: PubMed
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