Sign in to edit your profile (add interests, mentoring, photo, etc.)
    Keywords
    Last Name
    Institution

    Harvey M Florman PhD

    TitleProfessor
    InstitutionUniversity of Massachusetts Medical School
    DepartmentCell and Developmental Biology
    AddressUniversity of Massachusetts Medical School
    55 Lake Avenue North
    Worcester MA 01655
    Phone508-856-1675
      Other Positions
      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentBiochemistry and Molecular Pharmacology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentCell Biology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentInterdisciplinary Graduate Program

        Overview 
        Narrative

        Cell and Developmental Biology Department Website

        Academic Background

        1972 B.A., Rutgers College
        1982 Ph.D., University of Pennsylvania

        Mechanisms of Fertilization

        Fertilization is the process by which sperm and egg fuse and the program of development is initiated. This is controlled in mammals at two steps: 1) sperm first undergo “capacitation”, a physiological reprogramming that occurs within the female reproductive tract and that is essential for the expression of fertility; and 2) capacitated sperm interact with the egg. Our work has focused on the molecular and cellular mechanisms that control these two events. To this end, we apply a range of biophysical (single cell fluorescence imaging, patch clamp electrophysiology), biochemical and molecular techniques.

        Studies of capacitation address the control of sperm signal transduction pathways that lead to fertility. Ion fluxes are important regulators of this process and we have identified key roles of sperm membrane potential and pH. In addition, we recently recognized a role of polycystic kidney disease proteins in capacitation. Ongoing and future studies address the mechanisms by which factors from the female reproductive tract regulate these and other pathways, leading to sperm fertility.

        Sperm-egg interaction begins when sperm contact the egg extracellular matrix (the zona pellucida). ZP3, a protein component of the zona pellucida, activates sperm and triggers a secretory event known as the acrosome reaction. This is an obligatory event in gamete interaction that must be completed if sperm are to fuse with eggs, and disruption of the zona pellucida-evoked acrosome reaction is linked to several types of male infertility. We have worked on the signal transduction cascade that is regulated by ZP3. Specifically, we have focused on the intersecting roles of Ca2+ and Ca2+ channels, of phosphatidylinositol pathways, and protein kinases in the control of the acrosome reaction.



        Rotation Projects

        Potential Rotation/Thesis/Postdoctoral Projects

        1. Sperm ion channels: Patch clamping and ion imaging of sperm in order to study ion channel regulation.
        2. Function of polycystic kidney disease proteins in sperm: Structure/function analysis of sperm polycystins (PKD1-related and PKD2-related proteins), examining their role in sperm physiology, including capacitation.
        3. Sperm-egg communication: Signal transduction pathways in sperm that are activated by the egg and that lead to the control of sperm exocytosis.


        Bibliographic 
        selected publications
        List All   |   Timeline
        1. Florman HM, Cao W, Jungnickel MK, Sutton KA, Tsai PS. Coup d'oeil of sperm. Mol Reprod Dev. 2012 May; 79(5):Fmi.
          View in: PubMed
        2. Sutton KA, Jungnickel MK, Jovine L, Florman HM. Evolution of the Voltage Sensor Domain of the Voltage-Sensitive Phosphoinositide Phosphatase VSP/TPTE Suggests a Role as a Proton Channel in Eutherian Mammals. Mol Biol Evol. 2012 Sep; 29(9):2147-55.
          View in: PubMed
        3. Williams C, Florman H, Yanagamachi R. On the shoulders of giants: Robert G. Edwards, Nobel Laureate. Mol Reprod Dev. 2010 Dec; 77(12).
          View in: PubMed
        4. Kan MC, Oruganty-Das A, Cooper-Morgan A, Jin G, Swanger SA, Bassell GJ, Florman H, van Leyen K, Richter JD. CPEB4 is a cell survival protein retained in the nucleus upon ischemia or endoplasmic reticulum calcium depletion. Mol Cell Biol. 2010 Dec; 30(24):5658-71.
          View in: PubMed
        5. Visconti PE, Florman HM. Mechanisms of sperm-egg interactions: between sugars and broken bonds. Sci Signal. 2010; 3(142):pe35.
          View in: PubMed
        6. Florman HM, Jungnickel MK, Sutton KA. Shedding light on sperm pHertility. Cell. 2010 Feb 5; 140(3):310-2.
          View in: PubMed
        7. Sutton KA, Jungnickel MK, Florman HM. A polycystin-1 controls postcopulatory reproductive selection in mice. Proc Natl Acad Sci U S A. 2008 Jun 24; 105(25):8661-6.
          View in: PubMed
        8. Florman HM, Jungnickel MK, Sutton KA. Regulating the acrosome reaction. Int J Dev Biol. 2008; 52(5-6):503-10.
          View in: PubMed
        9. Florman HM, Jungnickel MK, Sutton KA. What can we learn about fertilization from cystic fibrosis? Proc Natl Acad Sci U S A. 2007 Jul 3; 104(27):11123-4.
          View in: PubMed
        10. Jungnickel MK, Sutton KA, Wang Y, Florman HM. Phosphoinositide-dependent pathways in mouse sperm are regulated by egg ZP3 and drive the acrosome reaction. Dev Biol. 2007 Apr 1; 304(1):116-26.
          View in: PubMed
        11. Sutton KA, Jungnickel MK, Ward CJ, Harris PC, Florman HM. Functional characterization of PKDREJ, a male germ cell-restricted polycystin. J Cell Physiol. 2006 Nov; 209(2):493-500.
          View in: PubMed
        12. Sutton KA, Jungnickel MK, Wang Y, Cullen K, Lambert S, Florman HM. Enkurin is a novel calmodulin and TRPC channel binding protein in sperm. Dev Biol. 2004 Oct 15; 274(2):426-35.
          View in: PubMed
        13. Galantino-Homer HL, Florman HM, Storey BT, Dobrinski I, Kopf GS. Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization on capacitation-associated protein tyrosine phosphorylation. Mol Reprod Dev. 2004 Apr; 67(4):487-500.
          View in: PubMed
        14. Sutton KA, Jungnickel MK, Florman HM. Of fertility, cystic fibrosis and the bicarbonate ion. Nat Cell Biol. 2003 Oct; 5(10):857-9.
          View in: PubMed
        15. Jungnickel MK, Sutton KA, Florman HM. In the beginning: lessons from fertilization in mice and worms. Cell. 2003 Aug 22; 114(4):401-4.
          View in: PubMed
        16. Kirkman-Brown JC, Sutton KA, Florman HM. How to attract a sperm. Nat Cell Biol. 2003 Feb; 5(2):93-6.
          View in: PubMed
        17. Evans JP, Florman HM. The state of the union: the cell biology of fertilization. Nat Cell Biol. 2002 Oct; 4 Suppl:s57-63.
          View in: PubMed
        18. Sutton KA, Jungnickel MK, Florman HM. If music be the food of love... Nat Cell Biol. 2002 Jun; 4(6):E154-5.
          View in: PubMed
        19. González-Martínez MT, Galindo BE, de De La Torre L, Zapata O, Rodríguez E, Florman HM, Darszon A. A sustained increase in intracellular Ca(2+) is required for the acrosome reaction in sea urchin sperm. Dev Biol. 2001 Aug 1; 236(1):220-9.
          View in: PubMed
        20. López-González I, De La Vega-Beltrán JL, Santi CM, Florman HM, Felix R, Darszon A. Calmodulin antagonists inhibit T-type Ca(2+) currents in mouse spermatogenic cells and the zona pellucida-induced sperm acrosome reaction. Dev Biol. 2001 Aug 1; 236(1):210-9.
          View in: PubMed
        21. Jungnickel MK, Marrero H, Birnbaumer L, Lémos JR, Florman HM. Trp2 regulates entry of Ca2+ into mouse sperm triggered by egg ZP3. Nat Cell Biol. 2001 May; 3(5):499-502.
          View in: PubMed
        22. Gross VS, Wessel G, Florman HM, Ducibella T. A monoclonal antibody that recognizes mammalian cortical granules and a 32-kilodalton protein in mouse eggs. Biol Reprod. 2000 Aug; 63(2):575-81.
          View in: PubMed
        23. O'Toole CM, Arnoult C, Darszon A, Steinhardt RA, Florman HM. Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction. Mol Biol Cell. 2000 May; 11(5):1571-84.
          View in: PubMed
        24. Arnoult C, Kazam IG, Visconti PE, Kopf GS, Villaz M, Florman HM. Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation. Proc Natl Acad Sci U S A. 1999 Jun 8; 96(12):6757-62.
          View in: PubMed
        25. Florman HM, Arnoult C, Kazam IG, Li C, O'Toole CM. A perspective on the control of mammalian fertilization by egg-activated ion channels in sperm: a tale of two channels. Biol Reprod. 1998 Jul; 59(1):12-6.
          View in: PubMed
        26. Arnoult C, Villaz M, Florman HM. Pharmacological properties of the T-type Ca2+ current of mouse spermatogenic cells. Mol Pharmacol. 1998 Jun; 53(6):1104-11.
          View in: PubMed
        27. Arnoult C, Lemos JR, Florman HM. Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation. EMBO J. 1997 Apr 1; 16(7):1593-9.
          View in: PubMed
        28. Arnoult C, Cardullo RA, Lemos JR, Florman HM. Activation of mouse sperm T-type Ca2+ channels by adhesion to the egg zona pellucida. Proc Natl Acad Sci U S A. 1996 Nov 12; 93(23):13004-9.
          View in: PubMed
        29. Arnoult C, Zeng Y, Florman HM. ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization. J Cell Biol. 1996 Aug; 134(3):637-45.
          View in: PubMed
        30. Zeng Y, Oberdorf JA, Florman HM. pH regulation in mouse sperm: identification of Na(+)-, Cl(-)-, and HCO3(-)-dependent and arylaminobenzoate-dependent regulatory mechanisms and characterization of their roles in sperm capacitation. Dev Biol. 1996 Feb 1; 173(2):510-20.
          View in: PubMed
        31. Zeng Y, Clark EN, Florman HM. Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion. Dev Biol. 1995 Oct; 171(2):554-63.
          View in: PubMed
        32. Florman HM. Sequential focal and global elevations of sperm intracellular Ca2+ are initiated by the zona pellucida during acrosomal exocytosis. Dev Biol. 1994 Sep; 165(1):152-64.
          View in: PubMed
        33. Clark EN, Corron ME, Florman HM. Caltrin, the calcium transport regulatory peptide of spermatozoa, modulates acrosomal exocytosis in response to the egg's zona pellucida. J Biol Chem. 1993 Mar 5; 268(7):5309-16.
          View in: PubMed
        34. Cardullo RA, Florman HM. Strategies and methods for evaluating acrosome reaction. Methods Enzymol. 1993; 225:136-53.
          View in: PubMed
        35. Florman HM, Corron ME, Kim TD, Babcock DF. Activation of voltage-dependent calcium channels of mammalian sperm is required for zona pellucida-induced acrosomal exocytosis. Dev Biol. 1992 Aug; 152(2):304-14.
          View in: PubMed
        36. Florman HM, Tombes RM, First NL, Babcock DF. An adhesion-associated agonist from the zona pellucida activates G protein-promoted elevations of internal Ca2+ and pH that mediate mammalian sperm acrosomal exocytosis. Dev Biol. 1989 Sep; 135(1):133-46.
          View in: PubMed
        37. Sirard MA, Florman HM, Leibfried-Rutledge ML, Barnes FL, Sims ML, First NL. Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. Biol Reprod. 1989 Jun; 40(6):1257-63.
          View in: PubMed
        38. Florman HM, First NL. Regulation of acrosomal exocytosis. II. The zona pellucida-induced acrosome reaction of bovine spermatozoa is controlled by extrinsic positive regulatory elements. Dev Biol. 1988 Aug; 128(2):464-73.
          View in: PubMed
        39. Florman HM, First NL. The regulation of acrosomal exocytosis. I. Sperm capacitation is required for the induction of acrosome reactions by the bovine zona pellucida in vitro. Dev Biol. 1988 Aug; 128(2):453-63.
          View in: PubMed
        40. Wassarman PM, Bleil JD, Florman HM, Greve JM, Roller RJ, Salzmann GS. Nature of the mouse egg's receptor for sperm. Adv Exp Med Biol. 1986; 207:55-77.
          View in: PubMed
        41. Florman HM, Wassarman PM. O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity. Cell. 1985 May; 41(1):313-24.
          View in: PubMed
        42. Wassarman PM, Bleil JD, Florman HM, Greve JM, Roller RJ, Salzmann GS, Samuels FG. The mouse egg's receptor for sperm: what is it and how does it work? Cold Spring Harb Symp Quant Biol. 1985; 50:11-9.
          View in: PubMed
        43. Florman HM, Bechtol KB, Wassarman PM. Enzymatic dissection of the functions of the mouse egg's receptor for sperm. Dev Biol. 1984 Nov; 106(1):243-55.
          View in: PubMed
        44. Florman HM, Storey BT. Mouse gamete interactions: the zona pellucida is the site of the acrosome reaction leading to fertilization in vitro. Dev Biol. 1982 May; 91(1):121-30.
          View in: PubMed
        45. Florman HM, Storey BT. Inhibition of in vitro fertilization of mouse eggs: 3-quinuclidinyl benzilate specifically blocks penetration of zonae pellucidae by mouse spermatozoa. J Exp Zool. 1981 Apr; 216(1):159-67.
          View in: PubMed
        For assistance with using Profiles, please refer to the online tutorials or contact UMMS Help Desk or call 508-856-8643.
        Harvey's Networks
        Click the "See All" links for more information and interactive visualizations!
        Concepts
        _
        Co-Authors
        _
        Similar People
        _
        Same Department
        Physical Neighbors
        _

        This is an official Page/Publication of the University of Massachusetts Worcester Campus
        Office of the Vice Provost for Research, 55 Lake Ave North, Worcester, Massachusetts 01655
        Questions or Comments? Email: publicaffairs@umassmed.edu Phone: 508-856-1572