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    Zdenka Matijasevic PhD

    TitleAssistant Professor
    InstitutionUniversity of Massachusetts Medical School
    DepartmentCell and Developmental Biology
    AddressUniversity of Massachusetts Medical School
    55 Lake Avenue North
    Worcester MA 01655
    Phone508-856-2459
      Other Positions
      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentBiochemistry and Molecular Pharmacology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentCell Biology

        Overview 
        Narrative
        Cell and Developmental Biology

        Academic Background

        BS, 1971; MSc, 1976; PhD, Zagreb, Croatia, 1982

        Role of MdmX in Cell Transformation and Tumorigenesis

        Photo: Zdenka Matijasevic MdmX is p53-binding proteins that functions as critical negative regulator of p53 activity in embryonic and adult tissue. Embryonic lethality caused by the loss of MdmX is completely rescued in p53-null background. Overexpression of MdmX was reported to inhibit p53 tumor suppressor functions in vitro, and amplification of MdmX is observed in variety of human cancers retaining wildtype p53. In contrast to the proposed oncogenic ability of overexpressed MdmX in p53 wildtype background, we found that MdmX suppresses tumorigenesis in mice deleted for p53 (Matijasevic, Steinman et al., 2008; Matijasevic et al., 2008). Loss of MdmX increases proliferation and spontaneous transformation of hyperploid p53-null cells in vitro. Increased proliferation correlates with reduction in chromosome number and with elevated multipolar mitotic spindle formation (see image) in both mouse embryonic fibroblasts and tumor cells. We now investigate molecular mechanisms involved in MdmX-mediated centrosome clustering that facilitates bipolar mitosis and its role in suppression of proliferation and tumorigenesis

        Cellular Responses to Hypothermia

        Mild hypothermia (28°C) increases the levels of tumor suppressor p53 protein in human fibroblasts and causes a p53-dependent cell cycle arrest in mouse fibroblasts; (Matijasevic et al., 1998). These findings suggest two areas of hypothermia application, cancer treatment and protection from environmental carcinogens.

        Hypothermia and Cancer Treatment

        Since many human tumors lack wild type p53 function, hypothermia may provide conditions for selective targeting of tumor cells; cell cycle arrest of normal cells at low temperature may protect them from cytotoxicity of drugs that target proliferating cells. Indeed, we found that, in contrast to p53-deficient cells, p53 wildtype cells survive much higher doses of drug 5-fluorouracil when incubated at 28°C than at 37°C (Matijasevic, 2002). Therefore, hypothermia may improve the therapeutic index of chemotherapy by the mechanisms based on the differences in cell cycle regulation between normal and tumor cells.

        Hypothermia and DNA Damage/Repair

        Acute and delayed toxicities from exposure to DNA-damaging agents such as sulfur mustard (SM) can be prevented or diminished by the activities of cellular DNA repair processes. At least two DNA repair mechanisms act upon SM-damaged DNA: base excision repair (BER) (Matijasevic et al., 1996) and nucleotide excision repair (NER) (Matijasevic et al., 2001). Surprisingly, activity of the first enzyme on BER pathway, DNA glycosylase, sensitizes cells to mustards (Matijasevic and Volkert, 2007). Low temperature improves recovery after the exposure to SM and the main component of this hypothermia-induced protection appears to be the inhibition of glycosylase activity.

        Figure

        p53 null and MdmX/p53 null

        MdmX prevents formation of multipolar spindles in p53-null cells.



        Bibliographic 
        selected publications
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        1. Lyle S, Hoover K, Colpan C, Zhu Z, Matijasevic Z, Jones SN. Dicer Cooperates with p53 to Suppress DNA Damage and Skin Carcinogenesis in Mice. PLoS One. 2014; 9(6):e100920.
          View in: PubMed
        2. Matijasevic Z, Krzywicka-Racka A, Sluder G, Jones SN. MdmX regulates transformation and chromosomal stability in p53-deficient cells. Cell Cycle. 2008 Oct; 7(19):2967-73.
          View in: PubMed
        3. Matijasevic Z, Steinman HA, Hoover K, Jones SN. MdmX promotes bipolar mitosis to suppress transformation and tumorigenesis in p53-deficient cells and mice. Mol Cell Biol. 2008 Feb; 28(4):1265-73.
          View in: PubMed
        4. Matijasevic Z, Volkert MR. Base excision repair sensitizes cells to sulfur mustard and chloroethyl ethyl sulfide. DNA Repair (Amst). 2007 Jun 1; 6(6):733-41.
          View in: PubMed
        5. Li Q, Wright SE, Matijasevic Z, Chong W, Ludlum DB, Volkert MR. The role of human alkyladenine glycosylase in cellular resistance to the chloroethylnitrosoureas. Carcinogenesis. 2003 Mar; 24(3):589-93.
          View in: PubMed
        6. Matijasevic Z. Selective protection of non-cancer cells by hypothermia. Anticancer Res. 2002 Nov-Dec; 22(6A):3267-72.
          View in: PubMed
        7. Bonanno K, Wyrzykowski J, Chong W, Matijasevic Z, Volkert MR. Alkylation resistance of E. coli cells expressing different isoforms of human alkyladenine DNA glycosylase (hAAG). DNA Repair (Amst). 2002 Jul 17; 1(7):507-16.
          View in: PubMed
        8. Matijasevic Z, Precopio ML, Snyder JE, Ludlum DB. Repair of sulfur mustard-induced DNA damage in mammalian cells measured by a host cell reactivation assay. Carcinogenesis. 2001 Apr; 22(4):661-4.
          View in: PubMed
        9. Ludlum DB, Li Q, Matijasevic Z. Role of base excision repair in protecting cells from the toxicity of chloroethylnitrosoureas. IARC Sci Publ. 1999; (150):271-7.
          View in: PubMed
        10. Matijasevic Z, Snyder JE, Ludlum DB. Hypothermia causes a reversible, p53-mediated cell cycle arrest in cultured fibroblasts. Oncol Res. 1998; 10(11-12):605-10.
          View in: PubMed
        11. Matijasevic Z, Stering A, Niu TQ, Austin-Ritchie P, Ludlum DB. Release of sulfur mustard-modified DNA bases by Escherichia coli 3-methyladenine DNA glycosylase II. Carcinogenesis. 1996 Oct; 17(10):2249-52.
          View in: PubMed
        12. Niu T, Matijasevic Z, Austin-Ritchie P, Stering A, Ludlum DB. A 32P-postlabeling method for the detection of adducts in the DNA of human fibroblasts exposed to sulfur mustard. Chem Biol Interact. 1996 Mar 8; 100(1):77-84.
          View in: PubMed
        13. Volkert MR, Hajec LI, Matijasevic Z, Fang FC, Prince R. Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene. J Bacteriol. 1994 Dec; 176(24):7638-45.
          View in: PubMed
        14. Matijasevic Z, Boosalis M, Mackay W, Samson L, Ludlum DB. Protection against chloroethylnitrosourea cytotoxicity by eukaryotic 3-methyladenine DNA glycosylase. Proc Natl Acad Sci U S A. 1993 Dec 15; 90(24):11855-9.
          View in: PubMed
        15. Matijasevic Z, Sekiguchi M, Ludlum DB. Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II. Proc Natl Acad Sci U S A. 1992 Oct 1; 89(19):9331-4.
          View in: PubMed
        16. Matijasevic Z, Hajec LI, Volkert MR. Anaerobic induction of the alkylation-inducible Escherichia coli aidB gene involves genes of the cysteine biosynthetic pathway. J Bacteriol. 1992 Mar; 174(6):2043-6.
          View in: PubMed
        17. Ludlum DB, Habraken Y, Carter CA, Matijasevic Z. The formation and enzymatic repair of DNA modifications caused by the haloethylnitrosoureas and related compounds. Nucleic Acids Symp Ser. 1992; (27):25-6.
          View in: PubMed
        18. Matijasevic Z, Bodell WJ, Ludlum DB. 3-Methyladenine DNA glycosylase activity in a glial cell line sensitive to the haloethylnitrosoureas in comparison with a resistant cell line. Cancer Res. 1991 Mar 1; 51(5):1568-70.
          View in: PubMed
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