Sign in to edit your profile (add interests, mentoring, photo, etc.)
    Keywords
    Last Name
    Institution

    Vivian Budnik PhD

    TitleChair and Professor
    InstitutionUniversity of Massachusetts Medical School
    DepartmentNeurobiology
    AddressUniversity of Massachusetts Medical School
    364 Plantation Street, LRB
    Worcester MA 01605
    Phone508-856-4341
      Other Positions
      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentCell Biology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentInterdisciplinary Graduate Program

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentMD/PhD Program

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentNeuroscience

        Overview 
        Narrative

        Molecular Mechanisms of Synapse Assembly and Plasticity

        The major goal of the research in our lab is to understand the mechanisms by which synapses are formed and modified. In the nervous system, the elemental means of communication between cells is synaptic transmission. Essential to the high speed and efficiency by which neurons communicate is the exquisite molecular organization of the pre- and postsynaptic apparatus. At the presynaptic compartment, neurotransmitter-laden vesicles are poised at active zones, ready for immediate release, and the calcium channels necessary for triggering exocytosis are spatially linked to the secretory machinery. At the postsynaptic membrane, neurotransmitter receptors form high-density clusters which are directly apposed to the active zones. Thus, proper development and functioning of synaptic junctions requires positional information, which coordinates the correct placement of pre- and postsynaptic elements. Once synapses are formed, it is the ability of synaptic connections to strengthen or to weaken which is believed to be central to the processes of learning and memory, and the restoration of synaptic connectivity after a traumatic injury.

        In the lab we are using a multidisciplinary approach that includes genetics, confocal and electron microscopy, electrophysiology and molecular biology to identify the proteins required to scaffold the synapse and to regulate it's molding during plasticity. Much of our studies are carried out using a Drosophila glutamatergic synapse model that has a high degree of evolutionary conservation with excitatory synapses in the mammalian brain. Using these approaches we have identified several proteins with fundamental roles in synapse formation and synapse dynamics, including Dlg, a member of the PSD-95 family, Wingless, a well-recognized factor for early embryonic pattern formation but with novel roles in synapse development, and APPL, the fly homolog of human APP, a protein with key roles in the development of Alzheimer's disease. This approach is proving to be extremely powerful in elucidating the cell biological mechanisms by which neuronal cell communication is established and modulated.

         



        Post Docs

        A postdoctoral position is available to study in this laboratory. Contact Dr. Budnik for additional details.



        Bibliographic 
        selected publications
        List All   |   Timeline
        1. Kerr KS, Fuentes-Medel Y, Brewer C, Barria R, Ashley J, Abruzzi KC, Sheehan A, Tasdemir-Yilmaz OE, Freeman MR, Budnik V. Glial wingless/wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction. J Neurosci. 2014 Feb 19; 34(8):2910-20.
          View in: PubMed
        2. Jokhi V, Ashley J, Nunnari J, Noma A, Ito N, Wakabayashi-Ito N, Moore MJ, Budnik V. Torsin mediates primary envelopment of large ribonucleoprotein granules at the nuclear envelope. Cell Rep. 2013 Apr 25; 3(4):988-95.
          View in: PubMed
        3. Korkut C, Li Y, Koles K, Brewer C, Ashley J, Yoshihara M, Budnik V. Regulation of postsynaptic retrograde signaling by presynaptic exosome release. Neuron. 2013 Mar 20; 77(6):1039-46.
          View in: PubMed
        4. Kalra H, Simpson RJ, Ji H, Aikawa E, Altevogt P, Askenase P, Bond VC, Borràs FE, Breakefield X, Budnik V, Buzas E, Camussi G, Clayton A, Cocucci E, Falcon-Perez JM, Gabrielsson S, Gho YS, Gupta D, Harsha HC, Hendrix A, Hill AF, Inal JM, Jenster G, Krämer-Albers EM, Lim SK, Llorente A, Lötvall J, Marcilla A, Mincheva-Nilsson L, Nazarenko I, Nieuwland R, Nolte-'t Hoen EN, Pandey A, Patel T, Piper MG, Pluchino S, Prasad TS, Rajendran L, Raposo G, Record M, Reid GE, Sánchez-Madrid F, Schiffelers RM, Siljander P, Stensballe A, Stoorvogel W, Taylor D, Thery C, Valadi H, van Balkom BW, Vázquez J, Vidal M, Wauben MH, Yáñez-Mó M, Zoeller M, Mathivanan S. Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation. PLoS Biol. 2012; 10(12):e1001450.
          View in: PubMed
        5. Fuentes-Medel Y, Ashley J, Barria R, Maloney R, Freeman M, Budnik V. Integration of a retrograde signal during synapse formation by glia-secreted TGF-ß ligand. Curr Biol. 2012 Oct 9; 22(19):1831-8.
          View in: PubMed
        6. Koles K, Budnik V. Wnt signaling in neuromuscular junction development. Cold Spring Harb Perspect Biol. 2012 Jun; 4(6).
          View in: PubMed
        7. Speese SD, Ashley J, Jokhi V, Nunnari J, Barria R, Li Y, Ataman B, Koon A, Chang YT, Li Q, Moore MJ, Budnik V. Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling. Cell. 2012 May 11; 149(4):832-46.
          View in: PubMed
        8. Koon AC, Budnik V. Inhibitory control of synaptic and behavioral plasticity by octopaminergic signaling. J Neurosci. 2012 May 2; 32(18):6312-22.
          View in: PubMed
        9. Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y, Leszyk J, Zhang B, Budnik V. Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. J Biol Chem. 2012 May 11; 287(20):16820-34.
          View in: PubMed
        10. Fainzilber M, Budnik V, Segal RA, Kreutz MR. From synapse to nucleus and back again--communication over distance within neurons. J Neurosci. 2011 Nov 9; 31(45):16045-8.
          View in: PubMed
        11. Budnik V, Salinas PC. Wnt signaling during synaptic development and plasticity. Curr Opin Neurobiol. 2011 Feb; 21(1):151-9.
          View in: PubMed
        12. Koon AC, Ashley J, Barria R, DasGupta S, Brain R, Waddell S, Alkema MJ, Budnik V. Autoregulatory and paracrine control of synaptic and behavioral plasticity by octopaminergic signaling. Nat Neurosci. 2011 Feb; 14(2):190-9.
          View in: PubMed
        13. Ramachandran P, Budnik V. Preparation of early Drosophila embryonic fillets (before cuticle deposition). Cold Spring Harb Protoc. 2010 Oct; 2010(10):pdb.prot5497.
          View in: PubMed
        14. Ramachandran P, Budnik V. Preparation of late Drosophila embryonic fillets. Cold Spring Harb Protoc. 2010 Oct; 2010(10):pdb.prot5498.
          View in: PubMed
        15. Dialynas G, Speese S, Budnik V, Geyer PK, Wallrath LL. The role of Drosophila Lamin C in muscle function and gene expression. Development. 2010 Sep; 137(18):3067-77.
          View in: PubMed
        16. Ramachandran P, Budnik V. Activity paradigm to induce Drosophila larval neuromuscular junction growth. Cold Spring Harb Protoc. 2010 Aug; 2010(8):pdb.prot5473.
          View in: PubMed
        17. Ramachandran P, Budnik V. Electron microscopy of Drosophila larval neuromuscular junctions. Cold Spring Harb Protoc. 2010 Aug; 2010(8):pdb.prot5474.
          View in: PubMed
        18. Ramachandran P, Budnik V. Internalization and trafficking assay for Drosophila larvae. Cold Spring Harb Protoc. 2010 Aug; 2010(8):pdb.prot5472.
          View in: PubMed
        19. Ramachandran P, Budnik V. Dissection of Drosophila larval body-wall muscles. Cold Spring Harb Protoc. 2010 Aug; 2010(8):pdb.prot5469.
          View in: PubMed
        20. Ramachandran P, Budnik V. Fm1-43 labeling of Drosophila larval neuromuscular junctions. Cold Spring Harb Protoc. 2010 Aug; 2010(8):pdb.prot5471.
          View in: PubMed
        21. Ramachandran P, Budnik V. Immunocytochemical staining of Drosophila larval body-wall muscles. Cold Spring Harb Protoc. 2010 Aug; 2010(8):pdb.prot5470.
          View in: PubMed
        22. Fuentes-Medel Y, Budnik V. Ménage à Trio during BMP-Mediated Retrograde Signaling at the NMJ. Neuron. 2010 May 27; 66(4):473-5.
          View in: PubMed
        23. Schulze SR, Curio-Penny B, Speese S, Dialynas G, Cryderman DE, McDonough CW, Nalbant D, Petersen M, Budnik V, Geyer PK, Wallrath LL. A comparative study of Drosophila and human A-type lamins. PLoS One. 2009; 4(10):e7564.
          View in: PubMed
        24. Korkut C, Ataman B, Ramachandran P, Ashley J, Barria R, Gherbesi N, Budnik V. Trans-synaptic transmission of vesicular Wnt signals through Evi/Wntless. Cell. 2009 Oct 16; 139(2):393-404.
          View in: PubMed
        25. Korkut C, Budnik V. WNTs tune up the neuromuscular junction. Nat Rev Neurosci. 2009 Sep; 10(9):627-34.
          View in: PubMed
        26. Fuentes-Medel Y, Logan MA, Ashley J, Ataman B, Budnik V, Freeman MR. Glia and muscle sculpt neuromuscular arbors by engulfing destabilized synaptic boutons and shed presynaptic debris. PLoS Biol. 2009 Aug; 7(8):e1000184.
          View in: PubMed
        27. Ramachandran P, Barria R, Ashley J, Budnik V. A critical step for postsynaptic F-actin organization: regulation of Baz/Par-3 localization by aPKC and PTEN. Dev Neurobiol. 2009 Aug; 69(9):583-602.
          View in: PubMed
        28. Ataman B, Ashley J, Gorczyca M, Ramachandran P, Fouquet W, Sigrist SJ, Budnik V. Rapid activity-dependent modifications in synaptic structure and function require bidirectional Wnt signaling. Neuron. 2008 Mar 13; 57(5):705-18.
          View in: PubMed
        29. Mendoza-Topaz C, Urra F, Barría R, Albornoz V, Ugalde D, Thomas U, Gundelfinger ED, Delgado R, Kukuljan M, Sanxaridis PD, Tsunoda S, Ceriani MF, Budnik V, Sierralta J. DLGS97/SAP97 is developmentally upregulated and is required for complex adult behaviors and synapse morphology and function. J Neurosci. 2008 Jan 2; 28(1):304-14.
          View in: PubMed
        30. Chai A, Withers J, Koh YH, Parry K, Bao H, Zhang B, Budnik V, Pennetta G. hVAPB, the causative gene of a heterogeneous group of motor neuron diseases in humans, is functionally interchangeable with its Drosophila homologue DVAP-33A at the neuromuscular junction. Hum Mol Genet. 2008 Jan 15; 17(2):266-80.
          View in: PubMed
        31. Li J, Ashley J, Budnik V, Bhat MA. Crucial role of Drosophila neurexin in proper active zone apposition to postsynaptic densities, synaptic growth, and synaptic transmission. Neuron. 2007 Sep 6; 55(5):741-55.
          View in: PubMed
        32. Speese SD, Budnik V. Wnts: up-and-coming at the synapse. Trends Neurosci. 2007 Jun; 30(6):268-75.
          View in: PubMed
        33. Gorczyca D, Ashley J, Speese S, Gherbesi N, Thomas U, Gundelfinger E, Gramates LS, Budnik V. Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin. J Neurosci. 2007 Jan 31; 27(5):1033-44.
          View in: PubMed
        34. Ataman B, Ashley J, Gorczyca D, Gorczyca M, Mathew D, Wichmann C, Sigrist SJ, Budnik V. Nuclear trafficking of Drosophila Frizzled-2 during synapse development requires the PDZ protein dGRIP. Proc Natl Acad Sci U S A. 2006 May 16; 103(20):7841-6.
          View in: PubMed
        35. Ataman B, Budnik V, Thomas U. Scaffolding proteins at the Drosophila neuromuscular junction. Int Rev Neurobiol. 2006; 75:181-216.
          View in: PubMed
        36. Budnik V, Gorczyca M, Prokop A. Selected methods for the anatomical study of Drosophila embryonic and larval neuromuscular junctions. Int Rev Neurobiol. 2006; 75:323-65.
          View in: PubMed
        37. Griffith LC, Budnik V. Plasticity and second messengers during synapse development. Int Rev Neurobiol. 2006; 75:237-65.
          View in: PubMed
        38. Ruiz-Cañada C, Budnik V. Synaptic cytoskeleton at the neuromuscular junction. Int Rev Neurobiol. 2006; 75:217-36.
          View in: PubMed
        39. Ruiz-Cañada C, Budnik V. Introduction on the use of the Drosophila embryonic/larval neuromuscular junction as a model system to study synapse development and function, and a brief summary of pathfinding and target recognition. Int Rev Neurobiol. 2006; 75:1-31.
          View in: PubMed
        40. Mathew D, Ataman B, Chen J, Zhang Y, Cumberledge S, Budnik V. Wingless signaling at synapses is through cleavage and nuclear import of receptor DFrizzled2. Science. 2005 Nov 25; 310(5752):1344-7.
          View in: PubMed
        41. Ashley J, Packard M, Ataman B, Budnik V. Fasciclin II signals new synapse formation through amyloid precursor protein and the scaffolding protein dX11/Mint. J Neurosci. 2005 Jun 22; 25(25):5943-55.
          View in: PubMed
        42. Ruiz-Canada C, Ashley J, Moeckel-Cole S, Drier E, Yin J, Budnik V. New synaptic bouton formation is disrupted by misregulation of microtubule stability in aPKC mutants. Neuron. 2004 May 27; 42(4):567-80.
          View in: PubMed
        43. Mathew D, Popescu A, Budnik V. Drosophila amphiphysin functions during synaptic Fasciclin II membrane cycling. J Neurosci. 2003 Nov 19; 23(33):10710-6.
          View in: PubMed
        44. Packard M, Mathew D, Budnik V. FASt remodeling of synapses in Drosophila. Curr Opin Neurobiol. 2003 Oct; 13(5):527-34.
          View in: PubMed
        45. Packard M, Mathew D, Budnik V. Wnts and TGF beta in synaptogenesis: old friends signalling at new places. Nat Rev Neurosci. 2003 Feb; 4(2):113-20.
          View in: PubMed
        46. Packard M, Koo ES, Gorczyca M, Sharpe J, Cumberledge S, Budnik V. The Drosophila Wnt, wingless, provides an essential signal for pre- and postsynaptic differentiation. Cell. 2002 Nov 1; 111(3):319-30.
          View in: PubMed
        47. Roche JP, Packard MC, Moeckel-Cole S, Budnik V. Regulation of synaptic plasticity and synaptic vesicle dynamics by the PDZ protein Scribble. J Neurosci. 2002 Aug 1; 22(15):6471-9.
          View in: PubMed
        48. Park D, Coleman MJ, Hodge JJ, Budnik V, Griffith LC. Regulation of neuronal excitability in Drosophila by constitutively active CaMKII. J Neurobiol. 2002 Jul; 52(1):24-42.
          View in: PubMed
        49. Mathew D, Gramates LS, Packard M, Thomas U, Bilder D, Perrimon N, Gorczyca M, Budnik V. Recruitment of scribble to the synaptic scaffolding complex requires GUK-holder, a novel DLG binding protein. Curr Biol. 2002 Apr 2; 12(7):531-9.
          View in: PubMed
        50. Koh YH, Ruiz-Canada C, Gorczyca M, Budnik V. The Ras1-mitogen-activated protein kinase signal transduction pathway regulates synaptic plasticity through fasciclin II-mediated cell adhesion. J Neurosci. 2002 Apr 1; 22(7):2496-504.
          View in: PubMed
        51. Thomas U, Ebitsch S, Gorczyca M, Koh YH, Hough CD, Woods D, Gundelfinger ED, Budnik V. Synaptic targeting and localization of discs-large is a stepwise process controlled by different domains of the protein. Curr Biol. 2000 Sep 21; 10(18):1108-17.
          View in: PubMed
        52. Koh YH, Gramates LS, Budnik V. Drosophila larval neuromuscular junction: molecular components and mechanisms underlying synaptic plasticity. Microsc Res Tech. 2000 Apr 1; 49(1):14-25.
          View in: PubMed
        53. Koh YH, Popova E, Thomas U, Griffith LC, Budnik V. Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation. Cell. 1999 Aug 6; 98(3):353-63.
          View in: PubMed
        54. Gorczyca M, Popova E, Jia XX, Budnik V. The gene mod(mdg4) affects synapse specificity and structure in Drosophila. J Neurobiol. 1999 Jun 5; 39(3):447-60.
          View in: PubMed
        55. Gramates LS, Budnik V. Assembly and maturation of the Drosophila larval neuromuscular junction. Int Rev Neurobiol. 1999; 43:93-117.
          View in: PubMed
        56. Budnik V. Synapse maturation and structural plasticity at Drosophila neuromuscular junctions. Curr Opin Neurobiol. 1996 Dec; 6(6):858-67.
          View in: PubMed
        57. Budnik V, Koh YH, Guan B, Hartmann B, Hough C, Woods D, Gorczyca M. Regulation of synapse structure and function by the Drosophila tumor suppressor gene dlg. Neuron. 1996 Oct; 17(4):627-40.
          View in: PubMed
        58. Guan B, Hartmann B, Kho YH, Gorczyca M, Budnik V. The Drosophila tumor suppressor gene, dlg, is involved in structural plasticity at a glutamatergic synapse. Curr Biol. 1996 Jun 1; 6(6):695-706.
          View in: PubMed
        59. Lahey T, Gorczyca M, Jia XX, Budnik V. The Drosophila tumor suppressor gene dlg is required for normal synaptic bouton structure. Neuron. 1994 Oct; 13(4):823-35.
          View in: PubMed
        60. Gorczyca MG, Phillis RW, Budnik V. The role of tinman, a mesodermal cell fate gene, in axon pathfinding during the development of the transverse nerve in Drosophila. Development. 1994 Aug; 120(8):2143-52.
          View in: PubMed
        61. Gorczyca M, Augart C, Budnik V. Insulin-like receptor and insulin-like peptide are localized at neuromuscular junctions in Drosophila. J Neurosci. 1993 Sep; 13(9):3692-704.
          View in: PubMed
        62. Jia XX, Gorczyca M, Budnik V. Ultrastructure of neuromuscular junctions in Drosophila: comparison of wild type and mutants with increased excitability. J Neurobiol. 1993 Aug; 24(8):1025-44.
          View in: PubMed
        63. Budnik V, Gorczyca M. SSB, an antigen that selectively labels morphologically distinct synaptic boutons at the Drosophila larval neuromuscular junction. J Neurobiol. 1992 Oct; 23(8):1054-65.
          View in: PubMed
        For assistance with using Profiles, please refer to the online tutorials or contact UMMS Help Desk or call 508-856-8643.
        Vivian's Networks
        Click the "See All" links for more information and interactive visualizations!
        Concepts
        _
        Co-Authors
        _
        Similar People
        _
        Same Department
        Physical Neighbors
        _

        This is an official Page/Publication of the University of Massachusetts Worcester Campus
        Office of the Vice Provost for Research, 55 Lake Ave North, Worcester, Massachusetts 01655
        Questions or Comments? Email: publicaffairs@umassmed.edu Phone: 508-856-1572