Trudy G Morrison PHD
Title Professor
Institution University of Massachusetts Medical School
Department Microbiology & Physiological Systems
Address University of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Telephone 508-856-6592
Email
Other Positions
Institution UMMS - Graduate School of Biomedical Sciences
Department Immunology & Virology

Institution UMMS - Graduate School of Biomedical Sciences
Department MD/PhD Program

Institution UMMS - Graduate School of Biomedical Sciences
Department Molecular Genetics & Microbiology

Institution UMMS - Programs, Centers and Institutes
Department Center for AIDS Research
Narrative

Academic Background

B.A. Wellesley College
Ph. D. Tufts University School of Medicine
Postdoctoral training at Massachusetts Institute of Technology

 

Dr. Trudy Morrison

Mechanisms of Paramyxovirus Membrane Fusion
Assembly of Paramyxoviruses
Development of Virus Vaccines

My laboratory is exploring the molecular mechanisms of paramyxovirus entry into susceptible cells and the assembly and release of infectious virus from infected cells.  Paramyxoviruses are simple negative-stranded, enveloped RNA viruses.  The two viral glycoproteins, the hemagglutinin-neuraminidase (HN) protein and the fusion (F) protein, mediate entry of the virus into cells.   The HN protein is the virus attachment protein and the F protein directly mediates the membrane fusion required for virus penetration. However, the HN protein is required to activate the fusion activity of the F protein.  Using Newcastle disease virus as a model paramyxovirus, my laboratory is exploring the molecular mechanisms involved in the activation of the fusion protein and the requirements for the conformational changes in the F protein required to mediate membrane fusion.

Release of progeny paramyxoviruses from infected cells requires the assembly of the structural components of the virion, the viral glycoproteins, the matrix protein, and the ribonucleoprotein core, followed by the budding of mature virus from surfaces of infected cells.  We are exploring the protein interactions required for the formation of assembly complexes, the cell domains involved in virus assembly, and the host cell contributions to virus assembly and release.

My laboratory is also exploring the potential of virus-like particles as vaccines for different paramyxoviruses.

Publications
1. Schmidt MR, McGinnes LW, Kenward SA, Willems KN, Woodland RT, Morrison TG. Long-Term and Memory Immune Responses in Mice against Newcastle Disease Virus-Like Particles Containing Respiratory Syncytial Virus Glycoprotein Ectodomains. J Virol. 2012 Nov; 86(21):11654-62.
  View in: PubMed
 
2. Battisti AJ, Meng G, Winkler DC, McGinnes LW, Plevka P, Steven AC, Morrison TG, Rossmann MG. Structure and assembly of a paramyxovirus matrix protein. Proc Natl Acad Sci U S A. 2012 Aug 28; 109(35):13996-4000.
  View in: PubMed
 
3. Gravel KA, McGinnes LW, Reitter J, Morrison TG. The transmembrane domain sequence affects the structure and function of the newcastle disease virus fusion protein. J Virol. 2011 Apr; 85(7):3486-97.
  View in: PubMed
 
4. McGinnes LW, Gravel KA, Finberg RW, Kurt-Jones EA, Massare MJ, Smith G, Schmidt MR, Morrison TG. Assembly and immunological properties of newcastle disease virus-like particles containing the respiratory syncytial virus f and g proteins. J Virol. 2011 Jan; 85(1):366-77.
  View in: PubMed
 
5. McGinnes LW, Pantua H, Laliberte JP, Gravel KA, Jain S, Morrison TG. Assembly and biological and immunological properties of Newcastle disease virus-like particles. J Virol. 2010 May; 84(9):4513-23.
  View in: PubMed
 
6. Murawski MR, McGinnes LW, Finberg RW, Kurt-Jones EA, Massare MJ, Smith G, Heaton PM, Fraire AE, Morrison TG. Newcastle disease virus-like particles containing respiratory syncytial virus G protein induced protection in BALB/c mice, with no evidence of immunopathology. J Virol. 2010 Jan; 84(2):1110-23.
  View in: PubMed
 
7. Jain S, McGinnes LW, Morrison TG. Role of thiol/disulfide exchange in newcastle disease virus entry. J Virol. 2009 Jan; 83(1):241-9.
  View in: PubMed
 
8. Jain S, McGinnes LW, Morrison TG. Overexpression of thiol/disulfide isomerases enhances membrane fusion directed by the Newcastle disease virus fusion protein. J Virol. 2008 Dec; 82(24):12039-48.
  View in: PubMed
 
9. Laliberte JP, McGinnes LW, Morrison TG. Incorporation of functional HN-F glycoprotein-containing complexes into newcastle disease virus is dependent on cholesterol and membrane lipid raft integrity. J Virol. 2007 Oct; 81(19):10636-48.
  View in: PubMed
 
10. Jain S, McGinnes LW, Morrison TG. Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein. J Virol. 2007 Mar; 81(5):2328-39.
  View in: PubMed
 
11. Laliberte JP, McGinnes LW, Peeples ME, Morrison TG. Integrity of membrane lipid rafts is necessary for the ordered assembly and release of infectious Newcastle disease virus particles. J Virol. 2006 Nov; 80(21):10652-62.
  View in: PubMed
 
12. Pantua HD, McGinnes LW, Peeples ME, Morrison TG. Requirements for the assembly and release of Newcastle disease virus-like particles. J Virol. 2006 Nov; 80(22):11062-73.
  View in: PubMed
 
13. McGinnes LW, Pantua H, Reitter J, Morrison TG. Newcastle disease virus: propagation, quantification, and storage. Curr Protoc Microbiol. 2006 Jun; Chapter 15:Unit 15F.2.
  View in: PubMed
 
14. McGinnes LW, Morrison TG. Inhibition of receptor binding stabilizes Newcastle disease virus HN and F protein-containing complexes. J Virol. 2006 Mar; 80(6):2894-903.
  View in: PubMed
 
15. Pantua H, McGinnes LW, Leszyk J, Morrison TG. Characterization of an alternate form of Newcastle disease virus fusion protein. J Virol. 2005 Sep; 79(18):11660-70.
  View in: PubMed
 
16. Dolganiuc V, McGinnes L, Luna EJ, Morrison TG. Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts. J Virol. 2003 Dec; 77(24):12968-79.
  View in: PubMed
 
17. Gravel KA, Morrison TG. Interacting domains of the HN and F proteins of newcastle disease virus. J Virol. 2003 Oct; 77(20):11040-9.
  View in: PubMed
 
18. Morrison TG. Structure and function of a paramyxovirus fusion protein. Biochim Biophys Acta. 2003 Jul 11; 1614(1):73-84.
  View in: PubMed
 
19. McGinnes LW, Reitter JN, Gravel K, Morrison TG. Evidence for mixed membrane topology of the newcastle disease virus fusion protein. J Virol. 2003 Feb; 77(3):1951-63.
  View in: PubMed
 
20. McGinnes LW, Gravel K, Morrison TG. Newcastle disease virus HN protein alters the conformation of the F protein at cell surfaces. J Virol. 2002 Dec; 76(24):12622-33.
  View in: PubMed
 
21. McGinnes LW, Sergel T, Chen H, Hamo L, Schwertz S, Li D, Morrison TG. Mutational analysis of the membrane proximal heptad repeat of the newcastle disease virus fusion protein. Virology. 2001 Oct 25; 289(2):343-52.
  View in: PubMed
 
22. Sergel TA, McGinnes LW, Morrison TG. Mutations in the fusion peptide and adjacent heptad repeat inhibit folding or activity of the Newcastle disease virus fusion protein. J Virol. 2001 Sep; 75(17):7934-43.
  View in: PubMed
 
23. McGinnes L, Sergel T, Reitter J, Morrison T. Carbohydrate modifications of the NDV fusion protein heptad repeat domains influence maturation and fusion activity. Virology. 2001 May 10; 283(2):332-42.
  View in: PubMed
 
24. Morrison TG. The three faces of paramyxovirus attachment proteins. Trends Microbiol. 2001 Mar; 9(3):103-5.
  View in: PubMed
 
25. Sergel TA, McGinnes LW, Morrison TG. A single amino acid change in the Newcastle disease virus fusion protein alters the requirement for HN protein in fusion. J Virol. 2000 Jun; 74(11):5101-7.
  View in: PubMed
 
26. Young JK, Li D, Abramowitz MC, Morrison TG. Interaction of peptides with sequences from the Newcastle disease virus fusion protein heptad repeat regions. J Virol. 1999 Jul; 73(7):5945-56.
  View in: PubMed
 
27. Stone-Hulslander J, Morrison TG. Mutational analysis of heptad repeats in the membrane-proximal region of Newcastle disease virus HN protein. J Virol. 1999 May; 73(5):3630-7.
  View in: PubMed
 
28. Li Z, Sergel T, Razvi E, Morrison T. Effect of cleavage mutants on syncytium formation directed by the wild-type fusion protein of Newcastle disease virus. J Virol. 1998 May; 72(5):3789-95.
  View in: PubMed
 
29. Young JK, Hicks RP, Wright GE, Morrison TG. The role of leucine residues in the structure and function of a leucine zipper peptide inhibitor of paramyxovirus (NDV) fusion. Virology. 1998 Mar 30; 243(1):21-31.
  View in: PubMed
 
30. McGinnes LW, Morrison TG. Role of carbohydrate processing and calnexin binding in the folding and activity of the HN protein of Newcastle disease virus. Virus Res. 1998 Feb; 53(2):175-85.
  View in: PubMed
 
31. Young JK, Hicks RP, Wright GE, Morrison TG. Analysis of a peptide inhibitor of paramyxovirus (NDV) fusion using biological assays, NMR, and molecular modeling. Virology. 1997 Nov 24; 238(2):291-304.
  View in: PubMed
 
32. Stone-Hulslander J, Morrison TG. Detection of an interaction between the HN and F proteins in Newcastle disease virus-infected cells. J Virol. 1997 Sep; 71(9):6287-95.
  View in: PubMed
 
33. McGinnes LW, Morrison TG. Disulfide bond formation is a determinant of glycosylation site usage in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. J Virol. 1997 Apr; 71(4):3083-9.
  View in: PubMed
 
34. McGinnes LW, Morrison TG. Role of cotranslational disulfide bond formation in the folding of the hemagglutinin-neuraminidase protein of Newcastle disease virus. Virology. 1996 Oct 15; 224(2):465-76.
  View in: PubMed
 
35. Reitter JN, Sergel T, Morrison TG. Mutational analysis of the leucine zipper motif in the Newcastle disease virus fusion protein. J Virol. 1995 Oct; 69(10):5995-6004.
  View in: PubMed
 
36. McGinnes LW, Morrison TG. The role of individual oligosaccharide chains in the activities of the HN glycoprotein of Newcastle disease virus. Virology. 1995 Oct 1; 212(2):398-410.
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37. Sergel T, Morrison TG. Mutations in the cytoplasmic domain of the fusion glycoprotein of Newcastle disease virus depress syncytia formation. Virology. 1995 Jul 10; 210(2):264-72.
  View in: PubMed
 
38. McGinnes LW, Morrison TG. Modulation of the activities of HN protein of Newcastle disease virus by nonconserved cysteine residues. Virus Res. 1994 Dec; 34(3):305-16.
  View in: PubMed
 
39. Sergel-Germano T, McQuain C, Morrison T. Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion. J Virol. 1994 Nov; 68(11):7654-8.
  View in: PubMed
 
40. McGinnes LW, Morrison TG. The role of the individual cysteine residues in the formation of the mature, antigenic HN protein of Newcastle disease virus. Virology. 1994 May 1; 200(2):470-83.
  View in: PubMed
 
41. McGinnes LW, Morrison TG. Conformationally sensitive antigenic determinants on the HN glycoprotein of Newcastle disease virus form with different kinetics. Virology. 1994 Mar; 199(2):255-64.
  View in: PubMed
 
42. Sergel T, McGinnes L, Morrison T. Role of a conserved sequence in the maturation and function of the NDV HN glycoprotein. Virus Res. 1993 Dec; 30(3):281-94.
  View in: PubMed
 
43. Sergel T, McGinnes LW, Morrison TG. The fusion promotion activity of the NDV HN protein does not correlate with neuraminidase activity. Virology. 1993 Oct; 196(2):831-4.
  View in: PubMed
 
44. McGinnes L, Sergel T, Morrison T. Mutations in the transmembrane domain of the HN protein of Newcastle disease virus affect the structure and activity of the protein. Virology. 1993 Sep; 196(1):101-10.
  View in: PubMed
 
45. Sergel T, McGinnes LW, Peeples ME, Morrison TG. The attachment function of the Newcastle disease virus hemagglutinin-neuraminidase protein can be separated from fusion promotion by mutation. Virology. 1993 Apr; 193(2):717-26.
  View in: PubMed
 
46. Morrison T, McQuain C, Sergel T, McGinnes L, Reitter J. The role of the amino terminus of F1 of the Newcastle disease virus fusion protein in cleavage and fusion. Virology. 1993 Apr; 193(2):997-1000.
  View in: PubMed
 
47. Wang C, Raghu G, Morrison T, Peeples ME. Intracellular processing of the paramyxovirus F protein: critical role of the predicted amphipathic alpha helix adjacent to the fusion domain. J Virol. 1992 Jul; 66(7):4161-9.
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48. Morrison T, McQuain C, McGinnes L. Complementation between avirulent Newcastle disease virus and a fusion protein gene expressed from a retrovirus vector: requirements for membrane fusion. J Virol. 1991 Feb; 65(2):813-22.
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49. Morrison T, Hinshaw VS, Sheerar M, Cooley AJ, Brown D, McQuain C, McGinnes L. Retroviral expressed hemagglutinin-neuraminidase protein protects chickens from Newcastle disease virus induced disease. Microb Pathog. 1990 Dec; 9(6):387-96.
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50. Wilson C, Gilmore R, Morrison T. Aberrant membrane insertion of a cytoplasmic tail deletion mutant of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. Mol Cell Biol. 1990 Feb; 10(2):449-57.
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51. Morrison TG, McQuain C, O'Connell KF, McGinnes LW. Mature, cell-associated HN protein of Newcastle disease virus exists in two forms differentiated by posttranslational modifications. Virus Res. 1990 Feb; 15(2):113-33.
  View in: PubMed
 
52. Morrison TG, McGinnes LW. Avian cells expressing the Newcastle disease virus hemagglutinin-neuraminidase protein are resistant to Newcastle disease virus infection. Virology. 1989 Jul; 171(1):10-7.
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53. Wilson C, Connolly T, Morrison T, Gilmore R. Integration of membrane proteins into the endoplasmic reticulum requires GTP. J Cell Biol. 1988 Jul; 107(1):69-77.
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54. Morrison TG. Structure, function, and intracellular processing of paramyxovirus membrane proteins. Virus Res. 1988 May; 10(2-3):113-35.
  View in: PubMed
 
55. McGinnes L, McQuain C, Morrison T. The P protein and the nonstructural 38K and 29K proteins of Newcastle disease virus are derived from the same open reading frame. Virology. 1988 May; 164(1):256-64.
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56. McGinnes LW, Wilde A, Morrison TG. Nucleotide sequence of the gene encoding the Newcastle disease virus hemagglutinin-neuraminidase protein and comparisons of paramyxovirus hemagglutinin-neuraminidase protein sequences. Virus Res. 1987 May; 7(3):187-202.
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57. Wilson C, Gilmore R, Morrison T. Translation and membrane insertion of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. Mol Cell Biol. 1987 Apr; 7(4):1386-92.
  View in: PubMed
 
58. Morrison TG, Peeples ME, McGinnes LW. Conformational change in a viral glycoprotein during maturation due to disulfide bond disruption. Proc Natl Acad Sci U S A. 1987 Feb; 84(4):1020-4.
  View in: PubMed
 
59. McGinnes LW, Morrison TG. The nucleotide sequence of the gene encoding the Newcastle disease virus membrane protein and comparisons of membrane protein sequences. Virology. 1987 Feb; 156(2):221-8.
  View in: PubMed
 
60. McGinnes LW, Morrison TG. Nucleotide sequence of the gene encoding the Newcastle disease virus fusion protein and comparisons of paramyxovirus fusion protein sequences. Virus Res. 1986 Sep; 5(4):343-56.
  View in: PubMed
 
61. Wilde A, McQuain C, Morrison T. Identification of the sequence content of four polycistronic transcripts synthesized in Newcastle disease virus infected cells. Virus Res. 1986 Jul; 5(1):77-95.
  View in: PubMed
 
62. Morrison TG, McGinnes LJ. Cytochalasin D accelerates the release of Newcastle disease virus from infected cells. Virus Res. 1985 Dec; 4(1):93-106.
  View in: PubMed
 
63. McGinnes LW, Semerjian A, Morrison T. Conformational changes in Newcastle disease virus fusion glycoprotein during intracellular transport. J Virol. 1985 Nov; 56(2):341-8.
  View in: PubMed
 
64. Morrison T, Ward LJ, Semerjian A. Intracellular processing of the Newcastle disease virus fusion glycoprotein. J Virol. 1985 Mar; 53(3):851-7.
  View in: PubMed
 
65. Wilde A, Morrison T. Structural and functional characterization of Newcastle disease virus polycistronic RNA species. J Virol. 1984 Jul; 51(1):71-6.
  View in: PubMed
 
66. Morrison TG, Ward LJ. Intracellular processing of the vesicular stomatitis virus glycoprotein and the Newcastle disease virus hemagglutinin-neuraminidase glycoprotein. Virus Res. 1984; 1(3):225-39.
  View in: PubMed
 
67. Chatis PA, Morrison TG. Characterization of the soluble glycoprotein released from vesicular stomatitis virus-infected cells. J Virol. 1983 Jan; 45(1):80-90.
  View in: PubMed
 
68. Chatis PA, Morrison TG. Fatty acid modification of Newcastle disease virus glycoproteins. J Virol. 1982 Jul; 43(1):342-7.
  View in: PubMed
 
69. Chatis PA, Morrison TG. Mutational changes in the vesicular stomatitis virus glycoprotein affect the requirement of carbohydrate in morphogenesis. J Virol. 1981 Jan; 37(1):307-16.
  View in: PubMed
 
70. Morrison TG, Simpson D. Synthesis, stability, and cleavage of Newcastle disease virus glycoproteins in the absence of glycosylation. J Virol. 1980 Oct; 36(1):171-80.
  View in: PubMed
 
71. Chatis PA, Morrison TG. Vesicular stomatitis virus glycoprotein is anchored to intracellular membranes near its carboxyl end and is proteolytically cleaved at its amino terminus. J Virol. 1979 Mar; 29(3):957-63.
  View in: PubMed
 
72. Morrison TG, McQuain CO, Simpson D. Assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin. J Virol. 1978 Oct; 28(1):368-74.
  View in: PubMed
 
73. Morrison TG, McQuain CO. Assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes. J Virol. 1978 Apr; 26(1):115-25.
  View in: PubMed
 
74. Clinkscales CW, Bratt MA, Morrison TG. Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system. J Virol. 1977 Apr; 22(1):97-101.
  View in: PubMed
 
75. Morrison TG, McQuain C. Assembly of viral membranes. I. Association of vesicular stomatitis virus membrane proteins and membranes in a cell-free system. J Virol. 1977 Feb; 21(2):451-8.
  View in: PubMed
 
76. Hightower LE, Morrison TG, Bratt MA. Relationships among the polypeptides of Newcastle disease virus. J Virol. 1975 Dec; 16(6):1599-607.
  View in: PubMed
 
77. Morrison TG, Lodish HF. Recognition of protein synthesis initiation signals on bacteriophage ribonucleic acid by mammalian ribosomes. J Biol Chem. 1974 Sep 25; 249(18):5860-6.
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78. Morrison TG, Blumberg DD, Malamy MH. T7 protein synthesis in F' episome-containing cells: assignment of specific proteins to three translational groups. J Virol. 1974 Feb; 13(2):386-93.
  View in: PubMed
 
79. Morrison T, Stampfer M, Baltimore D, Lodish HF. Translation of vesicular stomatitis messenger RNA by extracts from mammalian and plant cells. J Virol. 1974 Jan; 13(1):62-72.
  View in: PubMed
 
80. Morrison TG, Lodish HF. Translation of bacteriophage Q RNA by cytoplasmic extracts of mammalian cells. Proc Natl Acad Sci U S A. 1973 Feb; 70(2):315-9.
  View in: PubMed
 
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Keyword
Last Name
Institution
    
 
 
 
Keywords   
Newcastle disease virus
Viral Fusion Proteins
HN Protein
Membrane Fusion
Viral Proteins
See all (258) keywords
Co-Authors  
Finberg, Robert
Kurt-Jones, Evelyn
Luna, Elizabeth
Schmidt, Madelyn
Wright, George
See all (8) people
Physical Neighbors  
Poteete, Anthony
Schrader, Carol
Jenness, Duane
Lemos, Jose
Schmidt, Madelyn

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