David S Lapointe PHD
Title Research Associate Professor
Institution University of Massachusetts Medical School
Department Cell and Developmental Biology
Address University of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Telephone 508-856-5141
Email
Other Positions
Institution UMMS - Graduate School of Biomedical Sciences
Department Bioinformatics & Computational Biology

Institution UMMS - Graduate School of Biomedical Sciences
Department Cell Biology

Institution UMMS - Programs, Centers and Institutes
Department Center for AIDS Research

Institution UMMS - Programs, Centers and Institutes
Department Information Services
Narrative

David Lapointe

Bioinformatics

My group provides support for scientific computing research at UMass Medical School. This includes support for high performance computing on our cluster, database and application development, as well as support for biostatistics and bioinformatics. Current projects are development and support for high throughput sequencing pipelines, application management and training for the HPC cluster, consulting and support for microarray and ChIP analysis, development and support for laboratory management systems, and custom program development. I develop desktop interfaces to bioinformatic tools, mostly using web technology. These involve server-side applications using perl, python, C/C++, as well as client-side applications with JAVA. The BioTools project which brings together many resources, one of which is the EMBOSS software suite, to provide support for the biomedical researchers and core facilities at UMass Medical School.

PubMed Abstract Tags

DSLapointe
Publications
1. Xie L, Gazin C, Park SM, Zhu LJ, Debily MA, Kittler EL, Zapp ML, Lapointe D, Gobeil S, Virbasius CM, Green MR. A Synthetic Interaction Screen Identifies Factors Selectively Required for Proliferation and TERT Transcription in p53-Deficient Human Cancer Cells. PLoS Genet. 2012 Dec; 8(12):e1003151.
  View in: PubMed
 
2. van der Deen M, Akech J, Lapointe D, Gupta S, Young DW, Montecino MA, Galindo M, Lian JB, Stein JL, Stein GS, van Wijnen AJ. Genomic Promoter Occupancy of Runt-related Transcription Factor RUNX2 in Osteosarcoma Cells Identifies Genes Involved in Cell Adhesion and Motility. J Biol Chem. 2012 Feb 10; 287(7):4503-17.
  View in: PubMed
 
3. Wong SM, Gawronski JD, Lapointe D, Akerley BJ. High-throughput insertion tracking by deep sequencing for the analysis of bacterial pathogens. Methods Mol Biol. 2011; 733:209-22.
  View in: PubMed
 
4. Zhu LJ, Christensen RG, Kazemian M, Hull CJ, Enuameh MS, Basciotta MD, Brasefield JA, Zhu C, Asriyan Y, Lapointe DS, Sinha S, Wolfe SA, Brodsky MH. FlyFactorSurvey: a database of Drosophila transcription factor binding specificities determined using the bacterial one-hybrid system. Nucleic Acids Res. 2011 Jan; 39(Database issue):D111-7.
  View in: PubMed
 
5. Zhu LJ, Gazin C, Lawson ND, Pagès H, Lin SM, Lapointe DS, Green MR. ChIPpeakAnno: a Bioconductor package to annotate ChIP-seq and ChIP-chip data. BMC Bioinformatics. 2010; 11:237.
  View in: PubMed
 
6. Lapointe DS. Genomic Analysis of Pluripotent Stem Cells. Human Stem Cell Technology and Biology: A Research Guide and Laboratory Manual, ed. GS Stein, et. al. 2010; 237.
 
7. Teplyuk NM, Galindo M, Teplyuk VI, Pratap J, Young DW, Lapointe D, Javed A, Stein JL, Lian JB, Stein GS, van Wijnen AJ. Runx2 regulates G protein-coupled signaling pathways to control growth of osteoblast progenitors. J Biol Chem. 2008 Oct 10; 283(41):27585-97.
  View in: PubMed
 
8. Young DW, Hassan MQ, Yang XQ, Galindo M, Javed A, Zaidi SK, Furcinitti P, Lapointe D, Montecino M, Lian JB, Stein JL, van Wijnen AJ, Stein GS. Mitotic retention of gene expression patterns by the cell fate-determining transcription factor Runx2. Proc Natl Acad Sci U S A. 2007 Feb 27; 104(9):3189-94.
  View in: PubMed
 
9. Jang T, Savarese T, Low HP, Kim S, Vogel H, Lapointe D, Duong T, Litofsky NS, Weimann JM, Ross AH, Recht L. Osteopontin expression in intratumoral astrocytes marks tumor progression in gliomas induced by prenatal exposure to N-ethyl-N-nitrosourea. Am J Pathol. 2006 May; 168(5):1676-85.
  View in: PubMed
 
10. Warke RV, Xhaja K, Martin KJ, Fournier MF, Shaw SK, Brizuela N, de Bosch N, Lapointe D, Ennis FA, Rothman AL, Bosch I. Dengue virus induces novel changes in gene expression of human umbilical vein endothelial cells. J Virol. 2003 Nov; 77(21):11822-32.
  View in: PubMed
 
11. Balint E, Lapointe D, Drissi H, van der Meijden C, Young DW, van Wijnen AJ, Stein JL, Stein GS, Lian JB. Phenotype discovery by gene expression profiling: mapping of biological processes linked to BMP-2-mediated osteoblast differentiation. J Cell Biochem. 2003 May 15; 89(2):401-26.
  View in: PubMed
 
12. van der Meijden CM, Lapointe DS, Luong MX, Peric-Hupkes D, Cho B, Stein JL, van Wijnen AJ, Stein GS. Gene profiling of cell cycle progression through S-phase reveals sequential expression of genes required for DNA replication and nucleosome assembly. Cancer Res. 2002 Jun 1; 62(11):3233-43.
  View in: PubMed
 
13. Lapointe DS, Olson MS. Compartmental analysis of 45Ca2+ efflux in perfused rat liver: effects of hormonal stimulation. Cell Calcium. 1991 Nov; 12(10):743-53.
  View in: PubMed
 
14. Lapointe DS, Olson MS. Platelet-activating factor-stimulated hepatic glycogenolysis is not mediated through cyclooxygenase-derived metabolites of arachidonic acid. J Biol Chem. 1989 Jul 25; 264(21):12130-3.
  View in: PubMed
 
15. Lapointe DS, Olson MS. Alteration of hepatic tissue spaces by platelet-activating factor and phenylephrine. Hepatology. 1989 Feb; 9(2):278-84.
  View in: PubMed
 
16. Lapointe DS, Hildebrandt E, Buxton DB, Patel TB, Waymack PP, Olson MS. Measurement of branched-chain alpha-keto acid dehydrogenase flux rates in perfused heart and liver. Methods Enzymol. 1988; 166:484-97.
  View in: PubMed
 
17. Fisher RA, Buxton DB, Lapointe DS, Hanahan DJ, Olson MS. AGEPC: a potent calcium-dependent chemical mediator in the liver. Adv Exp Med Biol. 1988; 232:203-12.
  View in: PubMed
 
18. Lapointe DS, Hanahan DJ, Olson MS. Mobilization of hepatic calcium pools by platelet activating factor. Biochemistry. 1987 Mar 24; 26(6):1568-74.
  View in: PubMed
 
19. Lapointe DS, Olson MS. alpha-Ketobutyrate metabolism in perfused rat liver: regulation of alpha-ketobutyrate decarboxylation and effects of alpha-ketobutyrate on pyruvate dehydrogenase. Arch Biochem Biophys. 1985 Nov 1; 242(2):417-29.
  View in: PubMed
 
20. Patel TB, Lapointe DS, Olson MS. The regulation of hepatic gluconeogenesis using alpha-ketoisovalerate and propionate as precursors. Arch Biochem Biophys. 1984 Sep; 233(2):362-9.
  View in: PubMed
 
21. Lapointe DS, Cohen RJ. Ornithine decarboxylase in Phycomyces: in vitro and in vivo properties. Arch Biochem Biophys. 1983 Jul 15; 224(2):515-25.
  View in: PubMed
 
22. Savageau MA, Lapointe DS. Optimization of kinetic proofreading: a general method for derivation of the constraint relations and an exploration of a specific case. J Theor Biol. 1981 Nov 7; 93(1):157-77.
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23. Lapointe DS, Cohen RJ. A rapid and efficient microassay of ornithine decarboxylase. Anal Biochem. 1980 Dec; 109(2):291-4.
  View in: PubMed
 
24. Francis G, LaPointe D, Eitzman DV, Rennert OM, Monif GR. Cord sera antitrypsin activity and the respiratory distress syndrome. Clin Pediatr (Phila). 1974 Jul; 13(7):600-2.
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25. Rennert OM, Julius RL, LaPointe D. RNA methylation, a possible genetic marker in cystic fibrosis. Pediatrics. 1972 Sep; 50(3):485-7.
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26. Rennert OM, Frias JL, Julius RL, Lapointe D. The detection of the heterozygote and homozygote in cystic fibrosis by short term lymphocyte culture studies: a defect in RNA methylation. Clin Pediatr (Phila). 1972 Jun; 11(6):351-2.
  View in: PubMed
 
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Keyword
Last Name
Institution
    
 
 
 
Keywords   
Liver
Platelet Activating Factor
Phenylephrine
Calcium
Oligonucleotide Array Sequence Analysis
See all (166) keywords
Co-Authors  
Javed, Amjad
Lian, Jane
Stein, Gary
Stein, Janet
Zhu, Lihua
See all (18) people
Physical Neighbors  
Andreadis, Athena
Keller, Jeanne
Woodhead, John
Hall-Anderson, Lisa
Morabito, Maria

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