Mitsuo Ikebe PHD
Title Professor
Institution University of Massachusetts Medical School
Department Microbiology & Physiological Systems
Address University of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Telephone 508-856-1954
Email
Other Positions
Institution UMMS - Graduate School of Biomedical Sciences
Department Biochemistry & Molecular Pharmacology

Institution UMMS - Graduate School of Biomedical Sciences
Department Cell Biology

Institution UMMS - Graduate School of Biomedical Sciences
Department Interdisciplinary Graduate Program

Institution UMMS - Programs, Centers and Institutes
Department Program in Cell Dynamics
Narrative

Academic Background

1975, B.S., Tokyo Institute of Technology
1977, M.S., Tokyo Institute of Technology
1982, Ph.D., Osaka University


Function and Regulation of Motor Proteins in Cellular Processes

Photo: Mitsuo IkebeMotor proteins play a fundamental role in diverse cellular processes, such as muscle contraction, cytokinesis, chemotaxis, phagocytosis, secretion, exocytosis, endocytosis, vesicular trafficking, etc. We wish to clarify the function and regulation of motor proteins in these cellular processes.

The goal of the research in my laboratory is two fold. One is to understand the events responsible for linking stimuli to contractile activity in smooth muscle as well as non-muscle cells at the molecular level. It is well known that a number of cellular events are regulated by protein kinases/phosphatases cascade. For vertebrate smooth muscle as well as non-muscle contractile machinery, the phosphorylation of myosin molecules is the predominant regulatory mechanism for contraction. While the protein kinases and phosphatases which catalyze these processes have been identified, the regulatory cascade of myosin phosphorylation /dephosphorylation is complex and has not been clarified. Although it is established that myosin phosphorylation is obligatory for initiation of smooth muscle contraction, it is not understood how myosin phosphorylation and the motility of non-muscle cells during cytokinesis , chemotaxis etc. are interrelated. Our objective is to clarify these problems.

The second goal of my research is to clarify the physiological function and regulation of mammalian unconventional myosins. It has recently become clear that myosin forms a large and diverse group of molecular motors which have been hypothesized to play fundamental roles in various cellular processes such as cell locomotion, phagocytosis and vesicle transport. Our goal is to identify the physiological function of each unconventional myosin and clarify the regulatory mechanism of these motor proteins.

Figure

Myosin
Publications
1. Zhang CH, Lifshitz LM, Uy KF, Ikebe M, Fogarty KE, Zhuge R. The cellular and molecular basis of bitter tastant-induced bronchodilation. PLoS Biol. 2013 Mar; 11(3):e1001501.
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2. Komatsu S, Dobson JG, Ikebe M, Shea LG, Fenton RA. Crosstalk between adenosine A(1) and ß(1) -adrenergic receptors regulates translocation of PKCe in isolated rat cardiomyocytes. J Cell Physiol. 2012 Sep; 227(9):3201-7.
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3. Schroeder HW, Hendricks AG, Ikeda K, Shuman H, Rodionov V, Ikebe M, Goldman YE, Holzbaur EL. Force-dependent detachment of Kinesin-2 biases track switching at cytoskeletal filament intersections. Biophys J. 2012 Jul 3; 103(1):48-58.
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4. Umeki N, Jung HS, Sakai T, Sato O, Ikebe R, Ikebe M. Phospholipid-dependent regulation of the motor activity of myosin X. Nat Struct Mol Biol. 2011; 18(7):783-8.
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5. Sakai T, Umeki N, Ikebe R, Ikebe M. Cargo binding activates myosin VIIA motor function in cells. Proc Natl Acad Sci U S A. 2011 Apr 26; 108(17):7028-33.
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6. Watanabe TM, Iwane AH, Tanaka H, Ikebe M, Yanagida T. Mechanical characterization of one-headed myosin-V using optical tweezers. PLoS One. 2010; 5(8):e12224.
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7. Komaba S, Watanabe S, Umeki N, Sato O, Ikebe M. Effect of phosphorylation in the motor domain of human myosin IIIA on its ATP hydrolysis cycle. Biochemistry. 2010 May 4; 49(17):3695-702.
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8. Sun Y, Schroeder HW, Beausang JF, Homma K, Ikebe M, Goldman YE. Myosin VI lever arm rotation: fixed or variable? Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):E63; author reply E64.
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9. Watanabe TM, Tokuo H, Gonda K, Higuchi H, Ikebe M. Myosin-X induces filopodia by multiple elongation mechanism. J Biol Chem. 2010 Jun 18; 285(25):19605-14.
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10. Sun Y, Sato O, Ruhnow F, Arsenault ME, Ikebe M, Goldman YE. Single-molecule stepping and structural dynamics of myosin X. Nat Struct Mol Biol. 2010 Apr; 17(4):485-91.
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11. Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB. The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol. 2009 Dec 28; 187(7):1117-32.
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12. Engel BD, Lechtreck KF, Sakai T, Ikebe M, Witman GB, Marshall WF. Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella. Methods Cell Biol. 2009; 93:157-77.
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13. Ressmeyer AR, Bai Y, Delmotte P, Uy KF, Thistlethwaite P, Fraire A, Sato O, Ikebe M, Sanderson MJ. Human airway contraction and formoterol-induced relaxation is determined by Ca2+ oscillations and Ca2+ sensitivity. Am J Respir Cell Mol Biol. 2010 Aug; 43(2):179-91.
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14. Ni S, Hong F, Brewer PD, Ikebe M, Onishi H, Baker JE, Facemyer KC, Cremo CR. Kinetic and motor functions mediated by distinct regions of the regulatory light chain of smooth muscle myosin. Biochim Biophys Acta. 2009 Nov; 1794(11):1599-605.
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15. Sugimoto Y, Sato O, Watanabe S, Ikebe R, Ikebe M, Wakabayashi K. Reverse conformational changes of the light chain-binding domain of myosin V and VI processive motor heads during and after hydrolysis of ATP by small-angle X-ray solution scattering. J Mol Biol. 2009 Sep 18; 392(2):420-35.
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16. Fenton RA, Komatsu S, Ikebe M, Shea LG, Dobson JG. Adenoprotection of the heart involves phospholipase C-induced activation and translocation of PKC-epsilon to RACK2 in adult rat and mouse. Am J Physiol Heart Circ Physiol. 2009 Aug; 297(2):H718-25.
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17. Umeki N, Jung HS, Watanabe S, Sakai T, Li XD, Ikebe R, Craig R, Ikebe M. The tail binds to the head-neck domain, inhibiting ATPase activity of myosin VIIA. Proc Natl Acad Sci U S A. 2009 May 26; 106(21):8483-8.
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18. Wang Z, Edwards JG, Riley N, Provance DW, Karcher R, Li XD, Davison IG, Ikebe M, Mercer JA, Kauer JA, Ehlers MD. Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell. 2008 Oct 31; 135(3):535-48.
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19. Watanabe S, Umeki N, Ikebe R, Ikebe M. Impacts of Usher syndrome type IB mutations on human myosin VIIa motor function. Biochemistry. 2008 Sep 9; 47(36):9505-13.
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20. Jung HS, Komatsu S, Ikebe M, Craig R. Head-head and head-tail interaction: a general mechanism for switching off myosin II activity in cells. Mol Biol Cell. 2008 Aug; 19(8):3234-42.
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21. Iwaki M, Iwane AH, Ikebe M, Yanagida T. Biased Brownian motion mechanism for processivity and directionality of single-headed myosin-VI. Biosystems. 2008 Jul-Aug; 93(1-2):39-47.
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22. Tanaka H, Homma K, White HD, Yanagida T, Ikebe M. Smooth muscle myosin phosphorylated at single head shows sustained mechanical activity. J Biol Chem. 2008 Jun 6; 283(23):15611-8.
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23. Li XD, Jung HS, Wang Q, Ikebe R, Craig R, Ikebe M. The globular tail domain puts on the brake to stop the ATPase cycle of myosin Va. Proc Natl Acad Sci U S A. 2008 Jan 29; 105(4):1140-5.
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24. Ikebe M. Regulation of the function of mammalian myosin and its conformational change. Biochem Biophys Res Commun. 2008 Apr 25; 369(1):157-64.
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25. Sun Y, Schroeder HW, Beausang JF, Homma K, Ikebe M, Goldman YE. Myosin VI walks "wiggly" on actin with large and variable tilting. Mol Cell. 2007 Dec 28; 28(6):954-64.
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26. Koga Y, Ikebe M. A novel regulatory mechanism of myosin light chain phosphorylation via binding of 14-3-3 to myosin phosphatase. Mol Biol Cell. 2008 Mar; 19(3):1062-71.
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27. Lu Y, Zhang H, Gokina N, Mandala M, Sato O, Ikebe M, Osol G, Fisher SA. Uterine artery myosin phosphatase isoform switching and increased sensitivity to SNP in a rat L-NAME model of hypertension of pregnancy. Am J Physiol Cell Physiol. 2008 Feb; 294(2):C564-71.
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28. Watanabe S, Watanabe TM, Sato O, Awata J, Homma K, Umeki N, Higuchi H, Ikebe R, Ikebe M. Human myosin Vc is a low duty ratio nonprocessive motor. J Biol Chem. 2008 Apr 18; 283(16):10581-92.
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29. Tokuo H, Mabuchi K, Ikebe M. The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation. J Cell Biol. 2007 Oct 22; 179(2):229-38.
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30. Komatsu S, Ikebe M. The phosphorylation of myosin II at the Ser1 and Ser2 is critical for normal platelet-derived growth factor induced reorganization of myosin filaments. Mol Biol Cell. 2007 Dec; 18(12):5081-90.
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31. Takizawa N, Ikebe R, Ikebe M, Luna EJ. Supervillin slows cell spreading by facilitating myosin II activation at the cell periphery. J Cell Sci. 2007 Nov 1; 120(Pt 21):3792-803.
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32. Nakamura K, Koga Y, Sakai H, Homma K, Ikebe M. cGMP-dependent relaxation of smooth muscle is coupled with the change in the phosphorylation of myosin phosphatase. Circ Res. 2007 Sep 28; 101(7):712-22.
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33. Ito K, Ikebe M, Kashiyama T, Mogami T, Kon T, Yamamoto K. Kinetic mechanism of the fastest motor protein, Chara myosin. J Biol Chem. 2007 Jul 6; 282(27):19534-45.
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34. Sato O, Li XD, Ikebe M. Myosin Va becomes a low duty ratio motor in the inhibited form. J Biol Chem. 2007 May 4; 282(18):13228-39.
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35. Okada T, Tanaka H, Iwane AH, Kitamura K, Ikebe M, Yanagida T. The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunits. Biochem Biophys Res Commun. 2007 Mar 9; 354(2):379-84.
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36. Oki E, Morita M, Kakeji Y, Ikebe M, Sadanaga N, Egasira A, Nishida K, Koga T, Ohata M, Honboh T, Yamamoto M, Baba H, Maehara Y. Salvage esophagectomy after definitive chemoradiotherapy for esophageal cancer. Dis Esophagus. 2007; 20(4):301-4.
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37. Takamoto N, Komatsu S, Komaba S, Niiro N, Ikebe M. Novel ZIP kinase isoform lacks leucine zipper. Arch Biochem Biophys. 2006 Dec 15; 456(2):194-203.
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38. Kambara T, Komaba S, Ikebe M. Human myosin III is a motor having an extremely high affinity for actin. J Biol Chem. 2006 Dec 8; 281(49):37291-301.
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39. Kargacin GJ, Hunt D, Emmett T, Rokolya A, McMartin GA, Wirch E, Walsh MP, Ikebe M, Kargacin ME. Localization of telokin at the intercalated discs of cardiac myocytes. Arch Biochem Biophys. 2006 Dec 15; 456(2):151-60.
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40. Li XD, Jung HS, Mabuchi K, Craig R, Ikebe M. The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motor. J Biol Chem. 2006 Aug 4; 281(31):21789-98.
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41. Nishikawa M, Nishikawa S, Inoue A, Iwane AH, Yanagida T, Ikebe M. A unique mechanism for the processive movement of single-headed myosin-IX. Biochem Biophys Res Commun. 2006 May 19; 343(4):1159-64.
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42. Watanabe S, Mabuchi K, Ikebe R, Ikebe M. Mechanoenzymatic characterization of human myosin Vb. Biochemistry. 2006 Feb 28; 45(8):2729-38.
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43. Iwaki M, Tanaka H, Iwane AH, Katayama E, Ikebe M, Yanagida T. Cargo-binding makes a wild-type single-headed myosin-VI move processively. Biophys J. 2006 May 15; 90(10):3643-52.
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44. Watanabe S, Ikebe R, Ikebe M. Drosophila myosin VIIA is a high duty ratio motor with a unique kinetic mechanism. J Biol Chem. 2006 Mar 17; 281(11):7151-60.
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45. Miyazaki K, Komatsu S, Ikebe M. Dynamics of RhoA and ROKalpha translocation in single living cells. Cell Biochem Biophys. 2006; 45(3):243-54.
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46. Payne MC, Zhang HY, Prosdocimo T, Joyce KM, Koga Y, Ikebe M, Fisher SA. Myosin phosphatase isoform switching in vascular smooth muscle development. J Mol Cell Cardiol. 2006 Feb; 40(2):274-82.
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47. Kambara T, Ikebe M. A unique ATP hydrolysis mechanism of single-headed processive myosin, myosin IX. J Biol Chem. 2006 Feb 24; 281(8):4949-57.
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48. Watanabe M, Nomura K, Ohyama A, Ishikawa R, Komiya Y, Hosaka K, Yamauchi E, Taniguchi H, Sasakawa N, Kumakura K, Ushiki T, Sato O, Ikebe M, Igarashi M. Myosin-Va regulates exocytosis through the submicromolar Ca2+-dependent binding of syntaxin-1A. Mol Biol Cell. 2005 Oct; 16(10):4519-30.
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49. Homma K, Ikebe M. Myosin X is a high duty ratio motor. J Biol Chem. 2005 Aug 12; 280(32):29381-91.
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50. Li XD, Ikebe R, Ikebe M. Activation of myosin Va function by melanophilin, a specific docking partner of myosin Va. J Biol Chem. 2005 May 6; 280(18):17815-22.
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51. Saeki N, Tokuo H, Ikebe M. BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity. J Biol Chem. 2005 Mar 18; 280(11):10128-34.
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52. Ikebe M, Li XD, Mabuchi K, Ikebe R. Conformational change and regulation of myosin molecules. Adv Exp Med Biol. 2005; 565:61-72; discussion 72, 359-69.
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53. Koga Y, Ikebe M. p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoA. J Biol Chem. 2005 Feb 11; 280(6):4983-91.
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54. Tokuo H, Ikebe M. Myosin X transports Mena/VASP to the tip of filopodia. Biochem Biophys Res Commun. 2004 Jun 18; 319(1):214-20.
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55. Watanabe TM, Tanaka H, Iwane AH, Maki-Yonekura S, Homma K, Inoue A, Ikebe R, Yanagida T, Ikebe M. A one-headed class V myosin molecule develops multiple large (approximately 32-nm) steps successively. Proc Natl Acad Sci U S A. 2004 Jun 29; 101(26):9630-5.
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56. Miyazaki K, Komatsu S, Ikebe M, Fenton RA, Dobson JG. Protein kinase Cepsilon and the antiadrenergic action of adenosine in rat ventricular myocytes. Am J Physiol Heart Circ Physiol. 2004 Oct; 287(4):H1721-9.
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57. Ali MY, Homma K, Iwane AH, Adachi K, Itoh H, Kinosita K, Yanagida T, Ikebe M. Unconstrained steps of myosin VI appear longest among known molecular motors. Biophys J. 2004 Jun; 86(6):3804-10.
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58. Sato O, White HD, Inoue A, Belknap B, Ikebe R, Ikebe M. Human deafness mutation of myosin VI (C442Y) accelerates the ADP dissociation rate. J Biol Chem. 2004 Jul 9; 279(28):28844-54.
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59. Komatsu S, Ikebe M. ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts. J Cell Biol. 2004 Apr 26; 165(2):243-54.
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60. Li XD, Mabuchi K, Ikebe R, Ikebe M. Ca2+-induced activation of ATPase activity of myosin Va is accompanied with a large conformational change. Biochem Biophys Res Commun. 2004 Mar 12; 315(3):538-45.
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61. Ajtai K, Garamszegi SP, Watanabe S, Ikebe M, Burghardt TP. The myosin cardiac loop participates functionally in the actomyosin interaction. J Biol Chem. 2004 May 28; 279(22):23415-21.
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62. Payne MC, Zhang HY, Shirasawa Y, Koga Y, Ikebe M, Benoit JN, Fisher SA. Dynamic changes in expression of myosin phosphatase in a model of portal hypertension. Am J Physiol Heart Circ Physiol. 2004 May; 286(5):H1801-10.
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63. Chen Y, Takizawa N, Crowley JL, Oh SW, Gatto CL, Kambara T, Sato O, Li XD, Ikebe M, Luna EJ. F-actin and myosin II binding domains in supervillin. J Biol Chem. 2003 Nov 14; 278(46):46094-106.
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64. Yanase M, Ikeda H, Ogata I, Matsui A, Noiri E, Tomiya T, Arai M, Inoue Y, Tejima K, Nagashima K, Nishikawa T, Shibata M, Ikebe M, Rojkind M, Fujiwara K. Functional diversity between Rho-kinase- and MLCK-mediated cytoskeletal actions in a myofibroblast-like hepatic stellate cell line. Biochem Biophys Res Commun. 2003 May 30; 305(2):223-8.
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65. Li XD, Ikebe M. Two functional heads are required for full activation of smooth muscle myosin. J Biol Chem. 2003 Aug 8; 278(32):29435-41.
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66. Komaba S, Inoue A, Maruta S, Hosoya H, Ikebe M. Determination of human myosin III as a motor protein having a protein kinase activity. J Biol Chem. 2003 Jun 13; 278(24):21352-60.
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67. Ikebe M, Inoue A, Nishikawa S, Homma K, Tanaka H, Iwane AH, Katayama E, Ikebe R, Yanagida T. Motor function of unconventional myosin. Adv Exp Med Biol. 2003; 538:143-56; discussion 157.
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68. Niiro N, Koga Y, Ikebe M. Agonist-induced changes in the phosphorylation of the myosin- binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscle. Biochem J. 2003 Jan 1; 369(Pt 1):117-28.
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69. Inoue A, Ikebe M. Characterization of the motor activity of mammalian myosin VIIA. J Biol Chem. 2003 Feb 14; 278(7):5478-87.
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70. Takizawa N, Koga Y, Ikebe M. Phosphorylation of CPI17 and myosin binding subunit of type 1 protein phosphatase by p21-activated kinase. Biochem Biophys Res Commun. 2002 Oct 4; 297(4):773-8.
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71. Takizawa N, Schmidt DJ, Mabuchi K, Villa-Moruzzi E, Tuft RA, Ikebe M. M20, the small subunit of PP1M, binds to microtubules. Am J Physiol Cell Physiol. 2003 Feb; 284(2):C250-62.
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72. Komatsu S, Miyazaki K, Tuft RA, Ikebe M. Translocation of telokin by cGMP signaling in smooth muscle cells. Am J Physiol Cell Physiol. 2002 Sep; 283(3):C752-61.
  View in: PubMed
 
73. Inoue A, Saito J, Ikebe R, Ikebe M. Myosin IXb is a single-headed minus-end-directed processive motor. Nat Cell Biol. 2002 Apr; 4(4):302-6.
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74. Inoue A, Sato O, Homma K, Ikebe M. DOC-2/DAB2 is the binding partner of myosin VI. Biochem Biophys Res Commun. 2002 Mar 29; 292(2):300-7.
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75. Takizawa N, Niiro N, Ikebe M. Dephosphorylation of the two regulatory components of myosin phosphatase, MBS and CPI17. FEBS Lett. 2002 Mar 27; 515(1-3):127-32.
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76. Nishikawa S, Homma K, Komori Y, Iwaki M, Wazawa T, Hikikoshi Iwane A, Saito J, Ikebe R, Katayama E, Yanagida T, Ikebe M. Class VI myosin moves processively along actin filaments backward with large steps. Biochem Biophys Res Commun. 2002 Jan 11; 290(1):311-7.
  View in: PubMed
 
77. Tanaka H, Homma K, Iwane AH, Katayama E, Ikebe R, Saito J, Yanagida T, Ikebe M. The motor domain determines the large step of myosin-V. Nature. 2002 Jan 10; 415(6868):192-5.
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78. Kawakami H, Higashihara M, Ohsaka M, Miyazaki K, Ikebe M, Hirano H. Myosin light chain phosphorylation is correlated with cold-induced changes in platelet shape. J Smooth Muscle Res. 2001 Dec; 37(5-6):113-22.
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79. Gillespie PG, Albanesi JP, Bahler M, Bement WM, Berg JS, Burgess DR, Burnside B, Cheney RE, Corey DP, Coudrier E, de Lanerolle P, Hammer JA, Hasson T, Holt JR, Hudspeth AJ, Ikebe M, Kendrick-Jones J, Korn ED, Li R, Mercer JA, Milligan RA, Mooseker MS, Ostap EM, Petit C, Pollard TD, Sellers JR, Soldati T, Titus MA. Myosin-I nomenclature. J Cell Biol. 2001 Nov 26; 155(5):703-4.
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80. Miyazaki K, Yano T, Schmidt DJ, Tokui T, Shibata M, Lifshitz LM, Kimura S, Tuft RA, Ikebe M. Rho-dependent agonist-induced spatio-temporal change in myosin phosphorylation in smooth muscle cells. J Biol Chem. 2002 Jan 4; 277(1):725-34.
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81. Homma K, Yoshimura M, Saito J, Ikebe R, Ikebe M. The core of the motor domain determines the direction of myosin movement. Nature. 2001 Aug 23; 412(6849):831-4.
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82. Yoshimura M, Homma K, Saito J, Inoue A, Ikebe R, Ikebe M. Dual regulation of mammalian myosin VI motor function. J Biol Chem. 2001 Oct 26; 276(43):39600-7.
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83. Tashima Y, Kohda Y, Nonoguchi H, Ikebe M, Machida K, Star RA, Tomita K. Intranephron localization and regulation of the V1a vasopressin receptor during chronic metabolic acidosis and dehydration in rats. Pflugers Arch. 2001 Aug; 442(5):652-61.
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84. Homma K, Saito J, Ikebe R, Ikebe M. Motor function and regulation of myosin X. J Biol Chem. 2001 Sep 7; 276(36):34348-54.
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85. Ikebe M, Komatsu S, Woodhead JL, Mabuchi K, Ikebe R, Saito J, Craig R, Higashihara M. The tip of the coiled-coil rod determines the filament formation of smooth muscle and nonmuscle myosin. J Biol Chem. 2001 Aug 10; 276(32):30293-300.
  View in: PubMed
 
86. Niiro N, Ikebe M. Zipper-interacting protein kinase induces Ca(2+)-free smooth muscle contraction via myosin light chain phosphorylation. J Biol Chem. 2001 Aug 3; 276(31):29567-74.
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87. Ikebe M, Nonoguchi H, Nakayama Y, Tashima Y, Tomita K. Upregulation of the secretory-type Na(+)/K(+)/2Cl(-)-cotransporter in the kidney by metabolic acidosis and dehydration in rats. J Am Soc Nephrol. 2001 Mar; 12(3):423-30.
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88. Hidalgo C, Craig R, Ikebe M, Padrón R. Mechanism of phosphorylation of the regulatory light chain of myosin from tarantula striated muscle. J Muscle Res Cell Motil. 2001; 22(1):51-9.
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89. Komatsu S, Yano T, Shibata M, Tuft RA, Ikebe M. Effects of the regulatory light chain phosphorylation of myosin II on mitosis and cytokinesis of mammalian cells. J Biol Chem. 2000 Nov 3; 275(44):34512-20.
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90. Homma K, Saito J, Ikebe R, Ikebe M. Ca(2+)-dependent regulation of the motor activity of myosin V. J Biol Chem. 2000 Nov 3; 275(44):34766-71.
  View in: PubMed
 
91. Moriya M, Katagiri C, Ikebe M, Yagi K. Immunohistochemical detection of calmodulin and calmodulin-dependent protein kinase II in the mouse testis. Zygote. 2000 Nov; 8(4):303-14.
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92. Yanase M, Ikeda H, Matsui A, Maekawa H, Noiri E, Tomiya T, Arai M, Yano T, Shibata M, Ikebe M, Fujiwara K, Rojkind M, Ogata I. Lysophosphatidic acid enhances collagen gel contraction by hepatic stellate cells: association with rho-kinase. Biochem Biophys Res Commun. 2000 Oct 14; 277(1):72-8.
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93. Li XD, Saito J, Ikebe R, Mabuchi K, Ikebe M. The interaction between the regulatory light chain domains on two heads is critical for regulation of smooth muscle myosin. Biochemistry. 2000 Mar 7; 39(9):2254-60.
  View in: PubMed
 
94. Ikebe R, Reardon S, Mitsui T, Ikebe M. Role of the N-terminal region of the regulatory light chain in the dephosphorylation of myosin by myosin light chain phosphatase. J Biol Chem. 1999 Oct 15; 274(42):30122-6.
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95. Kitazawa T, Takizawa N, Ikebe M, Eto M. Reconstitution of protein kinase C-induced contractile Ca2+ sensitization in triton X-100-demembranated rabbit arterial smooth muscle. J Physiol. 1999 Oct 1; 520 Pt 1:139-52.
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96. Ikebe M, Yamada M, Mabuchi K, Kambara T, Ikebe R. Registration of the rod is not critical for the phosphorylation-dependent regulation of smooth muscle myosin. Biochemistry. 1999 Aug 17; 38(33):10768-74.
  View in: PubMed
 
97. Kambara T, Rhodes TE, Ikebe R, Yamada M, White HD, Ikebe M. Functional significance of the conserved residues in the flexible hinge region of the myosin motor domain. J Biol Chem. 1999 Jun 4; 274(23):16400-6.
  View in: PubMed
 
98. Nakayama Y, Nonoguchi H, Kiyama S, Ikebe M, Tashima Y, Shimada K, Tanzawa K, Tomita K. Intranephron distribution and regulation of endothelin-converting enzyme-1 in cyclosporin A-induced acute renal failure in rats. J Am Soc Nephrol. 1999 Mar; 10(3):562-71.
  View in: PubMed
 
99. Li XD, Rhodes TE, Ikebe R, Kambara T, White HD, Ikebe M. Effects of mutations in the gamma-phosphate binding site of myosin on its motor function. J Biol Chem. 1998 Oct 16; 273(42):27404-11.
  View in: PubMed
 
100. Ikebe M, Yamada M, Mabuchi K, Kambara T, Ikebe R. A specific amino acid sequence at the head-rod junction is not critical for the phosphorylation-dependent regulation of smooth muscle myosin. Biochemistry. 1998 Sep 22; 37(38):13285-90.
  View in: PubMed
 
101. Ikebe M. [Structure and regulation of myosin light chain kinase]. Tanpakushitsu Kakusan Koso. 1998 Sep; 43(12 Suppl):1715-24.
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102. Maruta S, Mitsuhashi S, Yamada M, Ikebe M. ADP/vanadate mediated photocleavage of myosin light chain kinase at the autoinhibitory region. J Biochem. 1998 Sep; 124(3):557-64.
  View in: PubMed
 
103. Maruta S, Ohki T, Kambara T, Ikebe M. Characterization of the interaction of myosin with ATP analogues having the syn conformation with respect to the adenine-ribose bond. Eur J Biochem. 1998 Aug 15; 256(1):229-37.
  View in: PubMed
 
104. Scott-Woo GC, Walsh MP, Ikebe M, Kargacin GJ. Identification and localization of caldesmon in cardiac muscle. Biochem J. 1998 Aug 15; 334 ( Pt 1):161-70.
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105. Zhu T, Beckingham K, Ikebe M. High affinity Ca2+ binding sites of calmodulin are critical for the regulation of myosin Ibeta motor function. J Biol Chem. 1998 Aug 7; 273(32):20481-6.
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106. Ikebe M, Kambara T, Stafford WF, Sata M, Katayama E, Ikebe R. A hinge at the central helix of the regulatory light chain of myosin is critical for phosphorylation-dependent regulation of smooth muscle myosin motor activity. J Biol Chem. 1998 Jul 10; 273(28):17702-7.
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107. Maruta S, Henry GD, Ohki T, Kambara T, Sykes BD, Ikebe M. Analysis of stress in the active site of myosin accompanied by conformational changes in transient state intermediate complexes using photoaffinity labeling and 19F-NMR spectroscopy. Eur J Biochem. 1998 Mar 15; 252(3):520-9.
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108. Amano M, Chihara K, Nakamura N, Fukata Y, Yano T, Shibata M, Ikebe M, Kaibuchi K. Myosin II activation promotes neurite retraction during the action of Rho and Rho-kinase. Genes Cells. 1998 Mar; 3(3):177-88.
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109. Walker JW, Gilbert SH, Drummond RM, Yamada M, Sreekumar R, Carraway RE, Ikebe M, Fay FS. Signaling pathways underlying eosinophil cell motility revealed by using caged peptides. Proc Natl Acad Sci U S A. 1998 Feb 17; 95(4):1568-73.
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110. Sata M, Perlman H, Muruve DA, Silver M, Ikebe M, Libermann TA, Oettgen P, Walsh K. Fas ligand gene transfer to the vessel wall inhibits neointima formation and overrides the adenovirus-mediated T cell response. Proc Natl Acad Sci U S A. 1998 Feb 3; 95(3):1213-7.
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111. Sreekumar R, Ikebe M, Fay FS, Walker JW. Biologically active peptides caged on tyrosine. Methods Enzymol. 1998; 291:78-94.
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112. Chihara K, Amano M, Nakamura N, Yano T, Shibata M, Tokui T, Ichikawa H, Ikebe R, Ikebe M, Kaibuchi K. Cytoskeletal rearrangements and transcriptional activation of c-fos serum response element by Rho-kinase. J Biol Chem. 1997 Oct 3; 272(40):25121-7.
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113. Liedtke CM, Cole T, Ikebe M. Differential activation of PKC-delta and -zeta by alpha 1-adrenergic stimulation in human airway epithelial cells. Am J Physiol. 1997 Sep; 273(3 Pt 1):C937-43.
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114. Fisher SA, Ikebe M, Brozovich F. Endothelin-1 alters the contractile phenotype of cultured embryonic smooth muscle cells. Circ Res. 1997 Jun; 80(6):885-93.
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115. Sata M, Stafford WF, Mabuchi K, Ikebe M. The motor domain and the regulatory domain of myosin solely dictate enzymatic activity and phosphorylation-dependent regulation, respectively. Proc Natl Acad Sci U S A. 1997 Jan 7; 94(1):91-6.
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116. Smith PG, Moreno R, Ikebe M. Strain increases airway smooth muscle contractile and cytoskeletal proteins in vitro. Am J Physiol. 1997 Jan; 272(1 Pt 1):L20-7.
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117. Watanabe M, Kuwano H, Sadanaga N, Ikebe M, Mori M, Sugimachi K. Leiomyoma of the esophagus with special reference to the characteristics of this tumor in teenagers. Hepatogastroenterology. 1997 Jan-Feb; 44(13):164-9.
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118. Sata M, Ikebe M. Functional analysis of the mutations in the human cardiac beta-myosin that are responsible for familial hypertrophic cardiomyopathy. Implication for the clinical outcome. J Clin Invest. 1996 Dec 15; 98(12):2866-73.
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119. Sata M, Matsuura M, Ikebe M. Characterization of the motor and enzymatic properties of smooth muscle long S1 and short HMM: role of the two-headed structure on the activity and regulation of the myosin motor. Biochemistry. 1996 Aug 27; 35(34):11113-8.
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120. Ikebe M, Brozovich FV. Protein kinase C increases force and slows relaxation in smooth muscle: evidence for regulation of the myosin light chain phosphatase. Biochem Biophys Res Commun. 1996 Aug 14; 225(2):370-6.
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121. Gopal D, Pavlov DI, Levitsky DI, Ikebe M, Burke M. Chemomechanical transduction in the actomyosin molecular motor by 2',3'-dideoxydidehydro-ATP and characterization of its interaction with myosin subfragment 1 in the presence and absence of actin. Biochemistry. 1996 Aug 6; 35(31):10149-57.
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122. Ng KP, Kambara T, Matsuura M, Burke M, Ikebe M. Identification of myosin III as a protein kinase. Biochemistry. 1996 Jul 23; 35(29):9392-9.
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123. Tokui T, Brozovich F, Ando S, Ikebe M. Enhancement of smooth muscle contraction with protein phosphatase inhibitor 1: activation of inhibitor 1 by cGMP-dependent protein kinase. Biochem Biophys Res Commun. 1996 Mar 27; 220(3):777-83.
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124. Zhu T, Sata M, Ikebe M. Functional expression of mammalian myosin I beta: analysis of its motor activity. Biochemistry. 1996 Jan 16; 35(2):513-22.
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125. Fisher SA, Ikebe M. Developmental and tissue distribution of expression of nonmuscle and smooth muscle isoforms of myosin light chain kinase. Biochem Biophys Res Commun. 1995 Dec 14; 217(2):696-703.
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126. Higashihara M, So A, Miyazaki K, Kawakami H, Ikebe M. [Mechanism of morphological changes of platelets upon exposure to cold]. J Smooth Muscle Res. 1995 Dec; 31(6):483-4.
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127. Gopal D, Bobkov AA, Schwonek JP, Sanders CR, Ikebe M, Levitsky DI, Burke M. Structural basis for actomyosin chemomechanical transduction by non-nucleoside triphosphate analogues. Biochemistry. 1995 Sep 26; 34(38):12178-84.
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128. Zhou ZH, Ando S, Furutsuka D, Ikebe M. Characterization of Ca2+/calmodulin-dependent protein kinase II from smooth muscle. Biochem J. 1995 Sep 1; 310 ( Pt 2):517-25.
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129. Tanaka M, Ikebe R, Matsuura M, Ikebe M. Pseudosubstrate sequence may not be critical for autoinhibition of smooth muscle myosin light chain kinase. EMBO J. 1995 Jun 15; 14(12):2839-46.
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130. Smith PG, Tokui T, Ikebe M. Mechanical strain increases contractile enzyme activity in cultured airway smooth muscle cells. Am J Physiol. 1995 Jun; 268(6 Pt 1):L999-1005.
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131. Katayama E, Ikebe M. Mode of caldesmon binding to smooth muscle thin filament: possible projection of the amino-terminal of caldesmon from native thin filament. Biophys J. 1995 Jun; 68(6):2419-28.
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132. Matsu-ura M, Ikebe M. Requirement of the two-headed structure for the phosphorylation dependent regulation of smooth muscle myosin. FEBS Lett. 1995 Apr 24; 363(3):246-50.
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133. Tokui T, Ando S, Ikebe M. Autophosphorylation of smooth muscle myosin light chain kinase at its regulatory domain. Biochemistry. 1995 Apr 18; 34(15):5173-9.
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134. Higashihara M, Ikebe M. Inhibition of 20-kDa myosin light chain exchange by monoclonal antibodies against 17-kDa myosin light chain. FEBS Lett. 1995 Apr 17; 363(1-2):57-60.
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135. Sugimoto Y, Tokunaga M, Takezawa Y, Ikebe M, Wakabayashi K. Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by x-ray solution scattering. Biophys J. 1995 Apr; 68(4 Suppl):29S-33S; discussion 33S-34S.
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136. Katayama E, Scott-Woo G, Ikebe M. Effect of caldesmon on the assembly of smooth muscle myosin. J Biol Chem. 1995 Feb 24; 270(8):3919-25.
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137. Kuwano H, Sadanaga N, Watanabe M, Ikebe M, Mori M, Sugimachi K, Kawamoto K. Preoperative endoscopic clipping for determining the resection line in early carcinoma of the esophagus. J Am Coll Surg. 1995 Jan; 180(1):97-9.
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138. Iizuka K, Ikebe M, Somlyo AV, Somlyo AP. Introduction of high molecular weight (IgG) proteins into receptor coupled, permeabilized smooth muscle. Cell Calcium. 1994 Dec; 16(6):431-45.
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139. Sugimachi K, Watanabe M, Sadanaga N, Ikebe M, Mori M, Kitamura K, Matsufuji H, Kuwano H. Pre-operative estimation of complete resection for patients with oesophageal carcinoma. Surg Oncol. 1994 Dec; 3(6):327-34.
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140. Ikebe M, Reardon S, Schwonek JP, Sanders CR, Ikebe R. Structural requirement of the regulatory light chain of smooth muscle myosin as a substrate for myosin light chain kinase. J Biol Chem. 1994 Nov 11; 269(45):28165-72.
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141. Ikebe M, Ikebe R, Kamisoyama H, Reardon S, Schwonek JP, Sanders CR, Matsuura M. Function of the NH2-terminal domain of the regulatory light chain on the regulation of smooth muscle myosin. J Biol Chem. 1994 Nov 11; 269(45):28173-80.
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142. Sadanaga N, Kuwano H, Watanabe M, Ikebe M, Mori M, Maekawa S, Hashizume M, Kitano S, Sugimachi K. Laparoscopy-assisted surgery: a new technique for transhiatal esophageal dissection. Am J Surg. 1994 Oct; 168(4):355-7.
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143. Ikebe M, Reardon S, Mitani Y, Kamisoyama H, Matsuura M, Ikebe R. Involvement of the C-terminal residues of the 20,000-dalton light chain of myosin on the regulation of smooth muscle actomyosin. Proc Natl Acad Sci U S A. 1994 Sep 13; 91(19):9096-100.
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144. Siegman MJ, Mooers SU, Warren TB, Warshaw DM, Ikebe M, Butler TM. Comparison of the effects of 2,3-butanedione monoxime on force production, myosin light chain phosphorylation and chemical energy usage in intact and permeabilized smooth and skeletal muscles. J Muscle Res Cell Motil. 1994 Aug; 15(4):457-72.
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145. Masuo M, Reardon S, Ikebe M, Kitazawa T. A novel mechanism for the Ca(2+)-sensitizing effect of protein kinase C on vascular smooth muscle: inhibition of myosin light chain phosphatase. J Gen Physiol. 1994 Aug; 104(2):265-86.
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146. Zhou ZL, Ikebe M. New isoforms of Ca2+/calmodulin-dependent protein kinase II in smooth muscle. Biochem J. 1994 Apr 15; 299 ( Pt 2):489-95.
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147. Kawakami H, Higashihara M, Song XH, Kurokawa K, Ikebe M, Hirano H. Okadaic acid induces marked shape change of human platelets. J Smooth Muscle Res. 1994 Apr; 30(2):57-64.
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148. Sugimachi K, Watanabe M, Sadanaga N, Ikebe M, Kitamura K, Mori M, Kuwano H. Recent advances in the diagnosis and surgical treatment of patients with carcinoma of the esophagus. J Am Coll Surg. 1994 Apr; 178(4):363-8.
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149. Kuwano H, Yano K, Ohno S, Ikebe M, Kitamura K, Toh Y, Mori M, Sugimachi K. Dipyridamole inhibits early wound healing in rat skin incisions. J Surg Res. 1994 Mar; 56(3):267-70.
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150. Mitsui T, Kitazawa T, Ikebe M. Correlation between high temperature dependence of smooth muscle myosin light chain phosphatase activity and muscle relaxation rate. J Biol Chem. 1994 Feb 25; 269(8):5842-8.
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151. Zhu T, Ikebe M. A novel myosin I from bovine adrenal gland. FEBS Lett. 1994 Feb 14; 339(1-2):31-6.
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152. Kamisoyama H, Araki Y, Ikebe M. Mutagenesis of the phosphorylation site (serine 19) of smooth muscle myosin regulatory light chain and its effects on the properties of myosin. Biochemistry. 1994 Jan 25; 33(3):840-7.
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153. Ikebe M, Mitra S, Hartshorne DJ. Cleavage at site A, Glu-642 to Ser-643, of the gizzard myosin heavy chain decreases affinity for actin. J Biol Chem. 1993 Dec 5; 268(34):25948-51.
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154. Yano K, Araki Y, Hales SJ, Tanaka M, Ikebe M. Boundary of the autoinhibitory region of smooth muscle myosin light-chain kinase. Biochemistry. 1993 Nov 16; 32(45):12054-61.
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155. Komatsu H, Ikebe M. Affinity labelling of smooth-muscle myosin light-chain kinase with 5'-[p-(fluorosulphonyl)benzoyl]adenosine. Biochem J. 1993 Nov 15; 296 ( Pt 1):53-8.
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156. Kuwano H, Ikebe M, Baba K, Kitamura K, Toh Y, Matsuda H, Sugimachi K. Operative procedures of reconstruction after resection of esophageal cancer and the postoperative quality of life. World J Surg. 1993 Nov-Dec; 17(6):773-6.
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157. Henry GD, Maruta S, Ikebe M, Sykes BD. Observation of multiple myosin subfragment 1-ADP-fluoroberyllate complexes by 19F NMR spectroscopy. Biochemistry. 1993 Oct 5; 32(39):10451-6.
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158. Kuwano H, Watanabe M, Sadanaga N, Ikebe M, Mori M, Sugimachi K. Squamous epithelial dysplasia associated with squamous cell carcinoma of the esophagus. Cancer Lett. 1993 Aug 31; 72(3):141-7.
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159. Kuwano H, Matsushima T, Ikebe M, Baba K, Kitamura K, Toh Y, Adachi Y, Sugimachi K. Mediastinal drainage prevents fatal pyothorax from anastomotic leakage after intrathoracic anastomosis in reconstruction for carcinoma of the esophagus. Surg Gynecol Obstet. 1993 Aug; 177(2):131-4.
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160. Kuwano H, Sadanaga N, Watanabe M, Ikebe M, Mori M, Sugimachi K. [Multidisciplinary treatment of esophageal cancer]. Gan To Kagaku Ryoho. 1993 Jul; 20(9):1167-72.
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161. Hisanaga S, Yasugawa S, Yamakawa T, Miyamoto E, Ikebe M, Uchiyama M, Kishimoto T. Dephosphorylation of microtubule-binding sites at the neurofilament-H tail domain by alkaline, acid, and protein phosphatases. J Biochem. 1993 Jun; 113(6):705-9.
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162. Sugimachi K, Ikebe M, Kitamura K, Toh Y, Matsuda H, Kuwano H. Long-term results of esophagectomy for early esophageal carcinoma. Hepatogastroenterology. 1993 Jun; 40(3):203-6.
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163. Ikebe M, Kitamura K, Baba K, Morita M, Tsutsui S, Sugimachi K. DNA ploidy as a prognostic factor in early esophageal carcinoma. Hepatogastroenterology. 1993 Jun; 40(3):232-5.
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164. Kitamura K, Ikebe M, Morita M, Matsuda H, Kuwano H, Sugimachi K. The evaluation of submucosal carcinoma of the esophagus as a more advanced carcinoma. Hepatogastroenterology. 1993 Jun; 40(3):236-9.
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165. Maruta S, Ikebe M. Photoaffinity labelling of smooth-muscle myosin by methylanthraniloyl-8-azido-ATP. Biochem J. 1993 Jun 1; 292 ( Pt 2):439-44.
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166. Maruta S, Henry GD, Sykes BD, Ikebe M. Formation of the stable myosin-ADP-aluminum fluoride and myosin-ADP-beryllium fluoride complexes and their analysis using 19F NMR. J Biol Chem. 1993 Apr 5; 268(10):7093-100.
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167. Kuwano H, Sadanaga N, Watanabe M, Ikebe M, Mori M, Sugimachi K. [Clinical study of hyperthermia combined with chemotherapy in the treatment of carcinoma]. Gan To Kagaku Ryoho. 1993 Apr; 20(5):597-602.
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168. Alessi D, MacDougall LK, Sola MM, Ikebe M, Cohen P. The control of protein phosphatase-1 by targetting subunits. The major myosin phosphatase in avian smooth muscle is a novel form of protein phosphatase-1. Eur J Biochem. 1992 Dec 15; 210(3):1023-35.
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169. Yoshikai S, Ikebe M. Molecular cloning of the chicken gizzard telokin gene and cDNA. Arch Biochem Biophys. 1992 Dec; 299(2):242-7.
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170. Ikebe M, Reardon S, Fay FS. Primary structure required for the inhibition of smooth muscle myosin light chain kinase. FEBS Lett. 1992 Nov 9; 312(2-3):245-8.
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171. Sugimachi K, Kitamura K, Baba K, Ikebe M, Kuwano H. Endoscopic diagnosis of early carcinoma of the esophagus using Lugol's solution. Gastrointest Endosc. 1992 Nov-Dec; 38(6):657-61.
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172. Moreland S, Ikebe M, Hunt JT, Moreland RS. Peptide analogs of the pseudosubstrate domain of smooth muscle myosin light chain kinase inhibit actomyosin ATPase activity at concentrations that do not inhibit superprecipitation. Biochem Biophys Res Commun. 1992 Sep 30; 187(3):1279-84.
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174. Mitsui T, Inagaki M, Ikebe M. Purification and characterization of smooth muscle myosin-associated phosphatase from chicken gizzards. J Biol Chem. 1992 Aug 15; 267(23):16727-35.
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175. Higashihara M, Takahata K, Kurokawa K, Ikebe M. The inhibitory effects of okadaic acid on platelet function. FEBS Lett. 1992 Jul 28; 307(2):206-10.
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176. Gong MC, Cohen P, Kitazawa T, Ikebe M, Masuo M, Somlyo AP, Somlyo AV. Myosin light chain phosphatase activities and the effects of phosphatase inhibitors in tonic and phasic smooth muscle. J Biol Chem. 1992 Jul 25; 267(21):14662-8.
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177. Matsuda H, Kuwano H, Tsutsui S, Kitamura K, Baba K, Ikebe M, Sugimachi K. A complete response of an advanced oesophageal carcinoma treated with hyperthermic chemotherapy: a case report. Int J Hyperthermia. 1992 Jul-Aug; 8(4):423-9.
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178. Moreland S, Ikebe M, Hunt JT, Moreland RS. Substrate based inhibitors of smooth muscle myosin light chain kinase. Biochem Biophys Res Commun. 1992 May 29; 185(1):379-85.
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179. McCarron JG, McGeown JG, Reardon S, Ikebe M, Fay FS, Walsh JV. Calcium-dependent enhancement of calcium current in smooth muscle by calmodulin-dependent protein kinase II. Nature. 1992 May 7; 357(6373):74-7.
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182. Fay FS, Yagi S, Itoh T, McCarron J, McGeown G, Walsh J, Ikebe M, Moore ED. Cellular and molecular physiology of calcium signalling in smooth muscle cells. Jpn J Pharmacol. 1992; 58 Suppl 2:35P-40P.
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185. Rajasekharan KN, Morita JI, Mayadevi M, Ikebe M, Burke M. Formation and properties of smooth muscle myosin 20-kDa light chain-skeletal muscle myosin hybrids and photocrosslinking from the maleimidylbenzophenone-labeled light chain to the heavy chain. Arch Biochem Biophys. 1991 Aug 1; 288(2):584-90.
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188. Araki Y, Ikebe M. Activation of smooth muscle myosin light chain kinase activity by a monoclonal antibody which recognizes the calmodulin-binding region. Biochem J. 1991 May 1; 275 ( Pt 3):679-84.
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189. Ikebe M, Hewett TE, Martin AF, Chen M, Hartshorne DJ. Cleavage of a smooth muscle myosin heavy chain near its C terminus by alpha-chymotrypsin. Effect on the properties of myosin. J Biol Chem. 1991 Apr 15; 266(11):7030-6.
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192. Draeger A, Amos WB, Ikebe M, Small JV. The cytoskeletal and contractile apparatus of smooth muscle: contraction bands and segmentation of the contractile elements. J Cell Biol. 1990 Dec; 111(6 Pt 1):2463-73.
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200. Ikebe M, Reardon S. Phosphorylation of bovine platelet myosin by protein kinase C. Biochemistry. 1990 Mar 20; 29(11):2713-20.
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221. Ikebe M, Hartshorne DJ. The role of myosin phosphorylation in the contraction-relaxation cycle of smooth muscle. Experientia. 1985 Aug 15; 41(8):1006-10.
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222. Ikebe M, Inagaki M, Kanamaru K, Hidaka H. Phosphorylation of smooth muscle myosin light chain kinase by Ca2+-activated, phospholipid-dependent protein kinase. J Biol Chem. 1985 Apr 25; 260(8):4547-50.
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223. Ikebe M, Hartshorne DJ. Proteolysis of smooth muscle myosin by Staphylococcus aureus protease: preparation of heavy meromyosin and subfragment 1 with intact 20 000-dalton light chains. Biochemistry. 1985 Apr 23; 24(9):2380-7.
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224. Srivastava S, Ikebe M, Hartshorne DJ. Trinitrophenylation of smooth muscle myosin. Biochem Biophys Res Commun. 1985 Jan 31; 126(2):748-55.
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225. Ikebe M, Hartshorne DJ. Conformation-dependent proteolysis of smooth-muscle myosin. J Biol Chem. 1984 Oct 10; 259(19):11639-42.
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226. Ikebe M, Barsotti RJ, Hinkins S, Hartshorne DJ. Effects of magnesium chloride on smooth muscle actomyosin adenosine-5'-triphosphatase activity, myosin conformation, and tension development in glycerinated smooth muscle fibers. Biochemistry. 1984 Oct 9; 23(21):5062-8.
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227. Ikebe M, Hinkins S, Hartshorne DJ. Correlation of intrinsic fluorescence and conformation of smooth muscle myosin. J Biol Chem. 1983 Dec 25; 258(24):14770-3.
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228. Ikebe M, Hinkins S, Hartshorne DJ. Correlation of enzymatic properties and conformation of smooth muscle myosin. Biochemistry. 1983 Sep 13; 22(19):4580-7.
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229. Takahashi K, Ogihara S, Ikebe M, Tonomura Y. Morphological aspects of thiophosphorylated and dephosphorylated myosin molecules from the plasmodium of Physarum polycephalum. J Biochem. 1983 Apr; 93(4):1175-83.
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230. Ogihara S, Ikebe M, Takahashi K, Tonomura Y. Requirement of phosphorylation of Physarum myosin heavy chain for thick filament formation, actin activation of Mg2+-ATPase activity, and Ca2+-inhibitory superprecipitation. J Biochem. 1983 Jan; 93(1):205-23.
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231. Ikebe M, Ogihara S. Phosphorylation-dependent and ATP-induced changes in structural array in gizzard myosin filament bundles. J Biochem. 1982 Dec; 92(6):1973-7.
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232. Ikebe M, Onishi H, Tonomura Y. Difference between smooth and skeletal muscle myosins in the stoichiometry of their reactions with ATP: identical and nonidentical two-headed structures of smooth and skeletal muscle myosins. J Biochem. 1982 Jun; 91(6):1855-73.
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233. Ikebe M, Ogihara S, Tonomura Y. Nonlinear dependence of actin-activated Mg2+-ATPase activity on the extent of phosphorylation of gizzard myosin and H-meromyosin. J Biochem. 1982 May; 91(5):1809-12.
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234. Ikebe M, Tonomura Y, Onishi H, Watanabe S. Elementary steps in the F-actin activated Mg2+-ATPase reaction of gizzard H-meromysin: effects of phosphorylation of the light-chain subunit. J Biochem. 1981 Jul; 90(1):61-77.
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235. Furukawa KI, Ikebe M, Inoue A, Tonomura Y. The amount of nucleotide binding and the P1-burst size of myosin adenosinetriphosphatase: evidence for the nonidentical two-headed structure of myosin. J Biochem. 1980 Dec; 88(6):1629-41.
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236. Ikebe M, Inoue A, Tonomura Y. Reaction mechanism of Mn2+-ATPase of acto-H-meromyosin in 0.1 M KCl at 5 degrees C: evidence for the Lymn-Taylor mechanism. J Biochem. 1980 Dec; 88(6):1653-62.
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237. Inoue A, Ikebe M, Tonomura Y. Mechanism of the Mg2+- and Mn2+-ATPase reactions of acto-H-meromyosin and acto-subfragment-1 in the absence of KCl at room temperature: direct decomposition of the complex of myosin-P-ADP with f-actin. J Biochem. 1980 Dec; 88(6):1663-77.
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238. Ikebe M, Aiba T, Onishi H, Watanabe S. Calcium sensitivity of contractile proteins from chicken gizzard muscle. J Biochem. 1978 Jun; 83(6):1643-55.
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239. Onishi H, Yamada Y, Ikebe M, Suzuki H, Watanabe S. The ATPase reaction in the steady state and in the initial burst catalyzed by chicken gizzard myosin in 0.6 M KCl1. J Biochem. 1978 Jan; 83(1):129-35.
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240. Ikebe M, Onishi H, Watanabe S. Phosphorylation and dephosphorylation of a light chain of the chicken gizzard myosin molecule. J Biochem. 1977 Jul; 82(1):299-302.
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241. Tamaura Y, Todokoro K, Ikebe M, Makino H, Hirose S. Fluorometric assay for discriminating the states of amino groups in insulin, lysozyme and asparaginase with fluorescamine. FEBS Lett. 1975 Jan 15; 50(1):70-3.
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Keyword
Last Name
Institution
    
 
 
 
Keywords   
Myosins
Muscle, Smooth
Actins
Myosin Heavy Chains
Molecular Motor Proteins
See all (658) keywords
Co-Authors  
Craig, Roger
Komatsu, Satoshi
Sato, Osamu
Takizawa, Norio
Watanabe, Shinya
See all (17) people
Physical Neighbors  
Goguen, Jon
Singer, Joshua
Baker, Richard
Wong, Sandy
Kowalik, Timothy

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