Clive Standley PHD
Title Research Associate Professor
Institution University of Massachusetts Medical School
Department Program in Molecular Medicine
Address University of Massachusetts Medical School
 373 Plantation Street
 Worcester MA 01605
Telephone 508-856-2133
Email
Other Positions
Institution UMMS - School of Medicine
Department Microbiology & Physiological Systems
Narrative

Academic Background

1983, BSc. Physics, Imperial College, University of London
1986, Ph.D. Physics, Imperial College, University of London

Development of Microscopy Techniques for Biomedical Imaging                                                                                Clive's Picture

My work involves the design of components to improve and extend the performance of the epi-fluorescence, total internal reflection (TIRF), and Biorad two photon microscopes in the Biomedical Imaging Group to answer the biological problems of interest to our collaborators.

TIRF Microscope

The microscope has been extensively use this year for the following programs:

Silvia Corvera and Debrah Leonard, UMass, to observe the specialization of plasma membrane domains for clathrin mediated endocytosis of transferrin.

Shaohui Huang, UMass, to observe how insulin regulates GluT4 vesicular trafficking near the plasma membrane of 3T3-L1 adipocytes.

John Walsh and Valerie DeCrescenzo, UMass, to observe exocytosis in nerve terminals and chromaffin cells.

Zheng Zheng Bao to image the action potential propagating across chicken embryo hearts in a bath containing a voltage sensitive fluorescent dye.

Xueyi Li, Mass General, to image GFP labeled Huntingtonâs protein.

Biorad Two Photon Microscope

A Biorad 2100MP two photon system has been installed and aligned. A new microscope stage has been constructed and a study of dendritic cells and mouse lymph nodes is under way in collaboration with Julie Mangada and Agata Jurczyk of UMass. A heated stage has been developed for the live mouse and a clamping system for the mouse skin flap is being made.
Publications
1. Zhang Y, Shi L, Li S, Yang Z, Standley C, Yang Z, Zhuge R, Savidge T, Wang X, Feng H. A Segment of 97 Amino Acids within the Translocation Domain of Clostridium difficile Toxin B Is Essential for Toxicity. PLoS One. 2013; 8(3):e58634.
  View in: PubMed
 
2. Guo D, Standley C, Bellve K, Fogarty K, Bao ZZ. Protein kinase Ca and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog. Mol Cell Neurosci. 2012 May; 50(1):82-92.
  View in: PubMed
 
3. Goel HL, Pursell B, Standley C, Fogarty K, Mercurio AM. Neuropilin-2 regulates a6ß1 integrin in the formation of focal adhesions and signaling. J Cell Sci. 2012 Feb 2.
  View in: PubMed
 
4. Navaroli DM, Bellvé KD, Standley C, Lifshitz LM, Cardia J, Lambright D, Leonard D, Fogarty KE, Corvera S. Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. Proc Natl Acad Sci U S A. 2012 Feb 21; 109(8):E471-80.
  View in: PubMed
 
5. Schmolze DB, Standley C, Fogarty KE, Fischer AH. Advances in microscopy techniques. Arch Pathol Lab Med. 2011 Feb; 135(2):255-63.
  View in: PubMed
 
6. Jurczyk A, Pino SC, O'Sullivan-Murphy B, Addorio M, Lidstone EA, Diiorio P, Lipson KL, Standley C, Fogarty K, Lifshitz L, Urano F, Mordes JP, Greiner DL, Rossini AA, Bortell R. A novel role for the centrosomal protein, pericentrin, in regulation of insulin secretory vesicle docking in mouse pancreatic beta-cells. PLoS One. 2010; 5(7):e11812.
  View in: PubMed
 
7. Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND. MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nature. 2010 Apr 22; 464(7292):1196-200.
  View in: PubMed
 
8. Siekmann AF, Standley C, Fogarty KE, Wolfe SA, Lawson ND. Chemokine signaling guides regional patterning of the first embryonic artery. Genes Dev. 2009 Oct 1; 23(19):2272-7.
  View in: PubMed
 
9. Li X, Standley C, Sapp E, Valencia A, Qin ZH, Kegel KB, Yoder J, Comer-Tierney LA, Esteves M, Chase K, Alexander J, Masso N, Sobin L, Bellve K, Tuft R, Lifshitz L, Fogarty K, Aronin N, DiFiglia M. Mutant huntingtin impairs vesicle formation from recycling endosomes by interfering with Rab11 activity. Mol Cell Biol. 2009 Nov; 29(22):6106-16.
  View in: PubMed
 
10. Kolpak AL, Jiang J, Guo D, Standley C, Bellve K, Fogarty K, Bao ZZ. Negative guidance factor-induced macropinocytosis in the growth cone plays a critical role in repulsive axon turning. J Neurosci. 2009 Aug 26; 29(34):10488-98.
  View in: PubMed
 
11. Leonard D, Hayakawa A, Lawe D, Lambright D, Bellve KD, Standley C, Lifshitz LM, Fogarty KE, Corvera S. Sorting of EGF and transferrin at the plasma membrane and by cargo-specific signaling to EEA1-enriched endosomes. J Cell Sci. 2008 Oct 15; 121(Pt 20):3445-58.
  View in: PubMed
 
12. Huang S, Lifshitz LM, Jones C, Bellve KD, Standley C, Fonseca S, Corvera S, Fogarty KE, Czech MP. Insulin stimulates membrane fusion and GLUT4 accumulation in clathrin coats on adipocyte plasma membranes. Mol Cell Biol. 2007 May; 27(9):3456-69.
  View in: PubMed
 
13. Hayakawa A, Leonard D, Murphy S, Hayes S, Soto M, Fogarty K, Standley C, Bellve K, Lambright D, Mello C, Corvera S. The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis. Proc Natl Acad Sci U S A. 2006 Aug 8; 103(32):11928-33.
  View in: PubMed
 
14. Bellve KD, Leonard D, Standley C, Lifshitz LM, Tuft RA, Hayakawa A, Corvera S, Fogarty KE. Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells. J Biol Chem. 2006 Jun 9; 281(23):16139-46.
  View in: PubMed
 
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Keyword
Last Name
Institution
    
 
 
 
Keywords   
Endosomes
Endocytosis
Zebrafish
Clathrin
Cell Membrane
See all (93) keywords
Co-Authors  
Bellve, Karl
Corvera, Silvia
Fogarty, Kevin
Lambright, David
Lifshitz, Lawrence
See all (23) people
Physical Neighbors  
Straubhaar, Juerg
Shirayama, Masaki
Kennedy, Norman
Bortell, Rita
Watanabe, Shinya

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