Anthony R Poteete PHD
Title Professor
Institution University of Massachusetts Medical School
Department Microbiology & Physiological Systems
Address University of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Telephone 508-856-3708
Email
Other Positions
Institution UMMS - Graduate School of Biomedical Sciences
Department Molecular Genetics & Microbiology

Institution UMMS - Programs, Centers and Institutes
Department Bacterial Genetics and Pathogenesis
Narrative

Academic Background

Ph. D. (1977) Massachusetts Institute of Technology

Genetic Recombination and Amplification


Homologous recombination is a universal life process, and our understanding of the process has been in large
part due to studies of recombination systems in Escherichia coli and its phages: RecA-RecBCD, the RecF pathway, the Rac prophage’s RecET, and the phage-encoded homologous recombination systems of phages lambda and T4.

Our studies focus on the phage lambda recombination system, which is called Red. Red is an especially active system, and has proven to be a valuable tool for genetic engineering in bacteria. Our main goal is to obtain a detailed understanding of the molecular mechanism of Red-mediated recombination. A secondary goal is the development of an understanding of the mechanisms of gene amplification.

Publications
1. Poteete AR. Recombination Phenotypes of Escherichia coli greA Mutants. BMC Mol Biol. 2011; 12:12.
  View in: PubMed
 
2. Poteete AR. Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions. BMC Mol Biol. 2009; 10:14.
  View in: PubMed
 
3. Poteete AR. Involvement of DNA replication in phage lambda Red-mediated homologous recombination. Mol Microbiol. 2008 Apr; 68(1):66-74.
  View in: PubMed
 
4. Stumpf JD, Poteete AR, Foster PL. Amplification of lac cannot account for adaptive mutation to Lac+ in Escherichia coli. J Bacteriol. 2007 Mar; 189(6):2291-9.
  View in: PubMed
 
5. Poteete AR, Rosadini C, St Pierre C. Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli. Biotechniques. 2006 Sep; 41(3):261-2, 264.
  View in: PubMed
 
6. Poteete AR, Fenton AC, Nadkarni A. Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12. BMC Mol Biol. 2004 Dec 13; 5(1):22.
  View in: PubMed
 
7. Poteete AR. Modulation of DNA repair and recombination by the bacteriophage lambda Orf function in Escherichia coli K-12. J Bacteriol. 2004 May; 186(9):2699-707.
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8. Pedulla ML, Ford ME, Karthikeyan T, Houtz JM, Hendrix RW, Hatfull GF, Poteete AR, Gilcrease EB, Winn-Stapley DA, Casjens SR. Corrected sequence of the bacteriophage p22 genome. J Bacteriol. 2003 Feb; 185(4):1475-7.
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9. Poteete AR, Fenton AC, Wang HR. Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12. J Bacteriol. 2002 Aug; 184(16):4626-9.
  View in: PubMed
 
10. Poteete AR, Wang HR, Foster PL. Phage lambda red-mediated adaptive mutation. J Bacteriol. 2002 Jul; 184(13):3753-5.
  View in: PubMed
 
11. Poteete AR. What makes the bacteriophage lambda Red system useful for genetic engineering: molecular mechanism and biological function. FEMS Microbiol Lett. 2001 Jul 10; 201(1):9-14.
  View in: PubMed
 
12. Murphy KC, Campellone KG, Poteete AR. PCR-mediated gene replacement in Escherichia coli. Gene. 2000 Apr 4; 246(1-2):321-30.
  View in: PubMed
 
13. Poteete AR, Fenton AC. Genetic requirements of phage lambda red-mediated gene replacement in Escherichia coli K-12. J Bacteriol. 2000 Apr; 182(8):2336-40.
  View in: PubMed
 
14. Wray JW, Baase WA, Lindstrom JD, Weaver LH, Poteete AR, Matthews BW. Structural analysis of a non-contiguous second-site revertant in T4 lysozyme shows that increasing the rigidity of a protein can enhance its stability. J Mol Biol. 1999 Oct 8; 292(5):1111-20.
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15. Poteete AR, Fenton AC, Murphy KC. Roles of RuvC and RecG in phage lambda red-mediated recombination. J Bacteriol. 1999 Sep; 181(17):5402-8.
  View in: PubMed
 
16. Jucovic M, Poteete AR. Protein salvage by directed evolution. Functional restoration of a defective lysozyme mutant. Ann N Y Acad Sci. 1999 May 18; 870:404-7.
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17. Jucovic M, Poteete AR. Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme. Protein Sci. 1998 Oct; 7(10):2200-9.
  View in: PubMed
 
18. Stahl MM, Thomason L, Poteete AR, Tarkowski T, Kuzminov A, Stahl FW. Annealing vs. invasion in phage lambda recombination. Genetics. 1997 Nov; 147(3):961-77.
  View in: PubMed
 
19. Poteete AR, Rennell D, Bouvier SE, Hardy LW. Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations. Protein Sci. 1997 Nov; 6(11):2418-25.
  View in: PubMed
 
20. Bouvier SE, Poteete AR. Second-site reversion of a structural defect in bacteriophage T4 lysozyme. FASEB J. 1996 Jan; 10(1):159-63.
  View in: PubMed
 
21. Summerford CM, Pardanani A, Betts AH, Poteete AR, Colotti G, Royer WE. Bacterial expression of Scapharca dimeric hemoglobin: a simple model system for investigating protein cooperatively. Protein Eng. 1995 Jun; 8(6):593-9.
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22. Poteete AR, Hardy LW. Genetic analysis of bacteriophage T4 lysozyme structure and function. J Bacteriol. 1994 Nov; 176(22):6783-8.
  View in: PubMed
 
23. Poteete AR, Fenton AC. Efficient double-strand break-stimulated recombination promoted by the general recombination systems of phages lambda and P22. Genetics. 1993 Aug; 134(4):1013-21.
  View in: PubMed
 
24. Ranade K, Poteete AR. Superinfection exclusion (sieB) genes of bacteriophages P22 and lambda. J Bacteriol. 1993 Aug; 175(15):4712-8.
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25. Ranade K, Poteete AR. A switch in translation mediated by an antisense RNA. Genes Dev. 1993 Aug; 7(8):1498-507.
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26. Rennell D, Poteete AR, Beaulieu M, Kuo DZ, Lew RA, Humphreys RE. Critical functional role of the COOH-terminal ends of longitudinal hydrophobic strips in alpha-helices of T4 lysozyme. J Biol Chem. 1992 Sep 5; 267(25):17748-52.
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27. Poteete AR, Rennell D, Bouvier SE. Functional significance of conserved amino acid residues. Proteins. 1992 May; 13(1):38-40.
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28. Rennell D, Bouvier SE, Hardy LW, Poteete AR. Systematic mutation of bacteriophage T4 lysozyme. J Mol Biol. 1991 Nov 5; 222(1):67-88.
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29. Hardy LW, Poteete AR. Reexamination of the role of Asp20 in catalysis by bacteriophage T4 lysozyme. Biochemistry. 1991 Oct 1; 30(39):9457-63.
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30. Poteete AR, Fenton AC, Semerjian AV. Bacteriophage P22 accessory recombination function. Virology. 1991 May; 182(1):316-23.
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31. Poteete AR, Sun DP, Nicholson H, Matthews BW. Second-site revertants of an inactive T4 lysozyme mutant restore activity by restructuring the active site cleft. Biochemistry. 1991 Feb 5; 30(5):1425-32.
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32. Rennell D, Poteete AR. Genetic analysis of bacteriophage P22 lysozyme structure. Genetics. 1989 Nov; 123(3):431-40.
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33. Casjens S, Eppler K, Parr R, Poteete AR. Nucleotide sequence of the bacteriophage P22 gene 19 to 3 region: identification of a new gene required for lysis. Virology. 1989 Aug; 171(2):588-98.
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34. Semerjian AV, Malloy DC, Poteete AR. Genetic structure of the bacteriophage P22 PL operon. J Mol Biol. 1989 May 5; 207(1):1-13.
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35. Poteete AR, Volkert MR. Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions. J Bacteriol. 1988 Sep; 170(9):4379-81.
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36. Poteete AR, Fenton AC, Murphy KC. Modulation of Escherichia coli RecBCD activity by the bacteriophage lambda Gam and P22 Abc functions. J Bacteriol. 1988 May; 170(5):2012-21.
  View in: PubMed
 
37. Knight JA, Hardy LW, Rennell D, Herrick D, Poteete AR. Mutations in an upstream regulatory sequence that increase expression of the bacteriophage T4 lysozyme gene. J Bacteriol. 1987 Oct; 169(10):4630-6.
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38. Murphy KC, Fenton AC, Poteete AR. Sequence of the bacteriophage P22 anti-recBCD (abc) genes and properties of P22 abc region deletion mutants. Virology. 1987 Oct; 160(2):456-64.
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39. Murphy KC, Casey L, Yannoutsos N, Poteete AR, Hendrix RW. Localization of a DNA-binding determinant in the bacteriophage P22 Erf protein. J Mol Biol. 1987 Mar 5; 194(1):105-17.
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40. Poteete AR, Hehir K, Sauer RT. Bacteriophage P22 Cro protein: sequence, purification, and properties. Biochemistry. 1986 Jan 14; 25(1):251-6.
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41. Weaver LH, Rennell D, Poteete AR, Mathews BW. Structure of phage P22 gene 19 lysozyme inferred from its homology with phage T4 lysozyme. Implications for lysozyme evolution. J Mol Biol. 1985 Aug 20; 184(4):739-41.
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42. Rennell D, Poteete AR. Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes. Virology. 1985 May; 143(1):280-9.
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43. Poteete AR, Fenton AC. Lambda red-dependent growth and recombination of phage P22. Virology. 1984 Apr 15; 134(1):161-7.
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44. Fenton AC, Poteete AR. Genetic analysis of the erf region of the bacteriophage P22 chromosome. Virology. 1984 Apr 15; 134(1):148-60.
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45. Marinus MG, Poteete A, Arraj JA. Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12. Gene. 1984 Apr; 28(1):123-5.
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46. Poteete AR, Sauer RT, Hendrix RW. Domain structure and quaternary organization of the bacteriophage P22 Erf protein. J Mol Biol. 1983 Dec 25; 171(4):401-18.
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47. Sauer RT, Nelson HC, Hehir K, Hecht MH, Gimble FS, DeAnda J, Poteete AR. The lambda and P22 phage repressors. J Biomol Struct Dyn. 1983 Dec; 1(4):1011-22.
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48. De Anda J, Poteete AR, Sauer RT. P22 c2 repressor. Domain structure and function. J Biol Chem. 1983 Sep 10; 258(17):10536-42.
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49. Berget PB, Poteete AR, Sauer RT. Control of phage P22 tail protein expression by transcription termination. J Mol Biol. 1983 Mar 15; 164(4):561-72.
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50. Poteete AR, Fenton AC. DNA-binding properties of the Erf protein of bacteriophage P22. J Mol Biol. 1983 Jan 15; 163(2):257-75.
  View in: PubMed
 
51. Sauer RT, Krovatin W, Poteete AR, Berget PB. Phage P22 tail protein: gene and amino acid sequence. Biochemistry. 1982 Nov 9; 21(23):5811-5.
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52. Poteete AR. Location and sequence of the erf gene of phage P22. Virology. 1982 Jun; 119(2):422-9.
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53. Poteete AR, Ptashne M. Control of transcription by the bacteriophage P22 repressor. J Mol Biol. 1982 May 5; 157(1):21-48.
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54. Johnson AD, Poteete AR, Lauer G, Sauer RT, Ackers GK, Ptashne M. lambda Repressor and cro--components of an efficient molecular switch. Nature. 1981 Nov 19; 294(5838):217-23.
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55. Sauer RT, Pan J, Hopper P, Hehir K, Brown J, Poteete AR. Primary structure of the phage P22 repressor and its gene c2. Biochemistry. 1981 Jun 9; 20(12):3591-8.
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56. Poteete AR, Roberts TM. Construction of plasmids that produce phage P22 repressor. Gene. 1981 Mar; 13(2):153-61.
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57. Roberts TM, Swanberg SL, Poteete A, Riedel G, Backman K. A plasmid cloning vehicle allowing a positive selection for inserted fragments. Gene. 1980 Dec; 12(1-2):123-7.
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58. Berget PB, Poteete AR. Structure and functions of the bacteriophage P22 tail protein. J Virol. 1980 Apr; 34(1):234-43.
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59. Poteete AR, Ptashne M, Ballivet M, Eisen H. Operator sequences of bacteriophages P22 and 21. J Mol Biol. 1980 Feb 15; 137(1):81-91.
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60. Poteete AR, Jarvik V, Botstein D. Encapsulation of phage P22 DNA in vitro. Virology. 1979 Jun; 95(2):550-64.
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61. Poteete AR, Botstein D. Purification and properties of proteins essential to DNA encapsulation by phage P22. Virology. 1979 Jun; 95(2):565-73.
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62. Botstein D, Poteete AR. Formation of P22 generalized transducing particles in vitro. Virology. 1979 Jun; 95(2):574-6.
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63. Poteete AR, King J. Functions of two new genes in Salmonella phage P22 assembly. Virology. 1977 Feb; 76(2):725-39.
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Keyword
Last Name
Institution
    
 
 
 
Keywords   
Recombination, Genetic
Bacteriophage lambda
Escherichia coli
Genes, Viral
Salmonella Phages
See all (174) keywords
Co-Authors  
Marinus, Martin
Murphy, Kenan
Royer, William
Volkert, Michael
See all (4) people
Physical Neighbors  
He, Feng
Dobson, James
Stavnezer, Janet
Amrani, Nadia
Baker, Richard

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