Header Logo

Erik J Sontheimer PhD

Endowed TitlePillar Chair in Biomedical Research
InstitutionUMass Chan Medical School
DepartmentRNA Therapeutics Institute
AddressUMass Chan Medical School
368 Plantation Street AS5.2051
Worcester MA 01605
vCardDownload vCard
    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionT.H. Chan School of Medicine
    DepartmentProgram in Molecular Medicine

    InstitutionT.H. Chan School of Medicine
    DepartmentRNA Therapeutics Institute

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentCancer Biology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentImmunology and Microbiology Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentPostbaccalaureate Research Education Program

    Collapse Biography 
    Collapse education and training
    Pennsylvania State University, College Park, PA, United StatesBSMolecular & Cell Biology
    Yale University, New Haven, CT, United StatesPHDMolecular Biophysics & Biochem

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG. Modular vector assembly enables rapid assessment of emerging CRISPR technologies. Cell Genom. 2024 Mar 13; 4(3):100519. PMID: 38484704.
    2. Bamidele N, Zhang H, Dong X, Cheng H, Gaston N, Feinzig H, Cao H, Kelly K, Watts JK, Xie J, Gao G, Sontheimer EJ. Domain-inlaid Nme2Cas9 adenine base editors with improved activity and targeting scope. Nat Commun. 2024 Feb 17; 15(1):1458. PMID: 38368418.
    3. Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, Amrani N, Chen Z, Gaston N, Wagh A, Newby GA, Xie J, Liu DR, Gao G, Wolfe SA, Khvorova A, Watts JK, Sontheimer EJ. Self-delivering, chemically modified CRISPR RNAs for AAV co-delivery and genome editing in vivo. Nucleic Acids Res. 2024 Jan 25; 52(2):977-997. PMID: 38033325.
      Citations:    Fields:    Translation:Animals
    4. Adler BA, Trinidad MI, Bellieny-Rabelo D, Zhang E, Karp HM, Skopintsev P, Thornton BW, Weissman RF, Yoon PH, Chen L, Hessler T, Eggers AR, Colognori D, Boger R, Doherty EE, Tsuchida CA, Tran RV, Hofman L, Shi H, Wasko KM, Zhou Z, Xia C, Al-Shimary MJ, Patel JR, Thomas VCJX, Pattali R, Kan MJ, Vardapetyan A, Yang A, Lahiri A, Maxwell MF, Murdock AG, Ramit GC, Henderson HR, Calvert RW, Bamert RS, Knott GJ, Lapinaite A, Pausch P, Cofsky JC, Sontheimer EJ, Wiedenheft B, Fineran PC, Brouns SJJ, Sashital DG, Thomas BC, Brown CT, Goltsman DSA, Barrangou R, Siksnys V, Banfield JF, Savage DF, Doudna JA. CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes. Nucleic Acids Res. 2024 Jan 05; 52(D1):D590-D596. PMID: 37889041.
      Citations: 1     Fields:    
    5. Chen Z, Kwan SY, Mir A, Hazeltine M, Shin M, Liang SQ, Chan IL, Kelly K, Ghanta KS, Gaston N, Cao Y, Xie J, Gao G, Xue W, Sontheimer EJ, Watts JK. A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors. CRISPR J. 2023 12; 6(6):570-582. PMID: 38108517.
      Citations:    Fields:    Translation:Animals
    6. Kraus C, Sontheimer EJ. Viruses use RNA decoys to thwart CRISPR defences. Nature. 2023 Nov; 623(7987):490-491. PMID: 37853195.
      Citations:    Fields:    Translation:Cells
    7. Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Joynt AT, Kelly K, Guo D, Chen Z, Lee JM, Schiffer CA, Emerson CP, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA. Addressing the dNTP bottleneck restricting prime editing activity. bioRxiv. 2023 Oct 29. PMID: 37904991.
    8. McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller NW, Steger RJ, Velasco BE, Bosch JA, Zirin JD, Viswanatha R, Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG. Modular vector assembly enables rapid assessment of emerging CRISPR technologies. bioRxiv. 2023 Oct 27. PMID: 37961518.
    9. Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodr?guez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P. PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun. 2023 10 04; 14(1):6175. PMID: 37794046.
      Citations:    Fields:    
    10. Liu B, Dong X, Zheng C, Keener D, Chen Z, Cheng H, Watts JK, Xue W, Sontheimer EJ. Targeted genome editing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates. Nat Biotechnol. 2023 Sep 14. PMID: 37709915.
      Citations: 2     Fields:    
    11. Zheng C, Liu B, Dong X, Gaston N, Sontheimer EJ, Xue W. Template-jumping prime editing enables large insertion and exon rewriting in vivo. Nat Commun. 2023 06 08; 14(1):3369. PMID: 37291100.
      Citations: 2     Fields:    Translation:Animals
    12. Liang SQ, Liu P, Ponnienselvan K, Suresh S, Chen Z, Kramme C, Chatterjee P, Zhu LJ, Sontheimer EJ, Xue W, Wolfe SA. Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag. Nat Methods. 2023 Jun; 20(6):898-907. PMID: 37156841.
      Citations: 5     Fields:    Translation:AnimalsCells
    13. Kraus C, Sontheimer EJ. Applications of Anti-CRISPR Proteins in Genome Editing and Biotechnology. J Mol Biol. 2023 07 01; 435(13):168120. PMID: 37100169.
      Citations: 3     Fields:    
    14. Bamidele N, Zhang H, Dong X, Gaston N, Cheng H, Kelly K, Watts JK, Xie J, Gao G, Sontheimer EJ. Engineering Nme2Cas9 Adenine Base Editors with Improved Activity and Targeting Scope. bioRxiv. 2023 Apr 18. PMID: 37131611.
    15. Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, Chen Z, Gaston N, Newby GA, Xie J, Liu DR, Gao G, Wolfe SA, Khvorova A, Watts JK, Sontheimer EJ. Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo. bioRxiv. 2023 Mar 23. PMID: 36993169.
    16. Koseki S, Hong L, Yudistyra V, Stan T, Tysinger E, Silverstein R, Kramme C, Amrani N, Savic N, Pacesa M, Rodriguez T, Ponnapati M, Jacobson J, Church G, Truant R, Jinek M, Kleinstiver B, Sontheimer E, Chatterjee P. PAM-Flexible Genome Editing with an Engineered Chimeric Cas9. Res Sq. 2023 Mar 07. PMID: 36945419.
    17. Meijboom KE, Abdallah A, Fordham NP, Nagase H, Rodriguez T, Kraus C, Gendron TF, Krishnan G, Esanov R, Andrade NS, Rybin MJ, Ramic M, Stephens ZD, Edraki A, Blackwood MT, Kahriman A, Henninger N, Kocher JA, Benatar M, Brodsky MH, Petrucelli L, Gao FB, Sontheimer EJ, Brown RH, Zeier Z, Mueller C. CRISPR/Cas9-mediated excision of ALS/FTD-causing hexanucleotide repeat expansion in C9ORF72 rescues major disease mechanisms in vivo and in vitro. Nat Commun. 2022 10 21; 13(1):6286. PMID: 36271076.
      Citations: 13     Fields:    Translation:AnimalsCells
    18. Rodr?guez TC, Kwan SY, Smith JL, Dadafarin S, Wu CH, Sontheimer EJ, Xue W. Multiomics characterization of mouse hepatoblastoma identifies yes-associated protein 1 target genes. Hepatology. 2023 07 01; 78(1):58-71. PMID: 35932276.
      Citations:    Fields:    Translation:HumansAnimalsCells
    19. Iyer S, Mir A, Vega-Badillo J, Roscoe BP, Ibraheim R, Zhu LJ, Lee J, Liu P, Luk K, Mintzer E, Guo D, Soares de Brito J, Emerson CP, Zamore PD, Sontheimer EJ, Wolfe SA. Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors. CRISPR J. 2022 10; 5(5):685-701. PMID: 36070530.
      Citations: 7     Fields:    Translation:HumansCells
    20. Shin M, Chan IL, Cao Y, Gruntman AM, Lee J, Sousa J, Rodr?guez TC, Echeverria D, Devi G, Debacker AJ, Moazami MP, Krishnamurthy PM, Rembetsy-Brown JM, Kelly K, Yukselen O, Donnard E, Parsons TJ, Khvorova A, Sontheimer EJ, Maehr R, Garber M, Watts JK. Intratracheally administered LNA gapmer antisense oligonucleotides induce robust gene silencing in mouse lung fibroblasts. Nucleic Acids Res. 2022 08 26; 50(15):8418-8430. PMID: 35920332.
      Citations: 6     Fields:    Translation:AnimalsCells
    21. Zhang H, Bamidele N, Liu P, Ojelabi O, Gao XD, Rodriguez T, Cheng H, Kelly K, Watts JK, Xie J, Gao G, Wolfe SA, Xue W, Sontheimer EJ. Adenine Base Editing In Vivo with a Single Adeno-Associated Virus Vector. GEN Biotechnol. 2022 Jun 01; 1(3):285-299. PMID: 35811581.
    22. Chen Z, Devi G, Arif A, Zamore PD, Sontheimer EJ, Watts JK. Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing. ACS Chem Biol. 2022 May 20; 17(5):1045-1050. PMID: 35446558.
      Citations: 2     Fields:    Translation:Humans
    23. Liu B, Dong X, Cheng H, Zheng C, Chen Z, Rodr?guez TC, Liang SQ, Xue W, Sontheimer EJ. A split prime editor with untethered reverse transcriptase and circular RNA template. Nat Biotechnol. 2022 09; 40(9):1388-1393. PMID: 35379962.
      Citations: 38     Fields:    Translation:Animals
    24. Liang SQ, Liu P, Smith JL, Mintzer E, Maitland S, Dong X, Yang Q, Lee J, Haynes CM, Zhu LJ, Watts JK, Sontheimer EJ, Wolfe SA, Xue W. Genome-wide detection of CRISPR editing in vivo using GUIDE-tag. Nat Commun. 2022 Jan 21; 13(1):437. PMID: 35064134.
      Citations: 13     Fields:    Translation:AnimalsCells
    25. Takasugi PR, Wang S, Truong KT, Drage EP, Kanishka SN, Higbee MA, Bamidele N, Ojelabi O, Sontheimer EJ, Gagnon JA. Orthogonal CRISPR-Cas tools for genome editing, inhibition, and CRISPR recording in zebrafish embryos. Genetics. 2022 01 04; 220(1). PMID: 34735006.
      Citations: 6     Fields:    
    26. Tsagkaraki E, Nicoloro SM, DeSouza T, Solivan-Rivera J, Desai A, Lifshitz LM, Shen Y, Kelly M, Guilherme A, Henriques F, Amrani N, Ibraheim R, Rodriguez TC, Luk K, Maitland S, Friedline RH, Tauer L, Hu X, Kim JK, Wolfe SA, Sontheimer EJ, Corvera S, Czech MP. CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease. Nat Commun. 2021 Nov 26; 12(1):6931. PMID: 34836963.
      Citations: 21     Fields:    Translation:HumansAnimalsCells
    27. Ibraheim R, Tai PWL, Mir A, Javeed N, Wang J, Rodr?guez TC, Namkung S, Nelson S, Khokhar ES, Mintzer E, Maitland S, Chen Z, Cao Y, Tsagkaraki E, Wolfe SA, Wang D, Pai AA, Xue W, Gao G, Sontheimer EJ. Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo. Nat Commun. 2021 11 01; 12(1):6267. PMID: 34725353.
      Citations: 31     Fields:    Translation:HumansAnimalsCells
    28. Ghanta KS, Chen Z, Mir A, Dokshin GA, Krishnamurthy PM, Yoon Y, Gallant J, Xu P, Zhang XO, Ozturk AR, Shin M, Idrizi F, Liu P, Gneid H, Edraki A, Lawson ND, Rivera-P?rez JA, Sontheimer EJ, Watts JK, Mello CC. 5'-Modifications improve potency and efficacy of DNA donors for precision genome editing. Elife. 2021 10 19; 10. PMID: 34665130.
      Citations: 22     Fields:    Translation:HumansAnimalsCells
    29. Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W. Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice. Nat Commun. 2021 04 09; 12(1):2121. PMID: 33837189.
      Citations: 98     Fields:    Translation:HumansAnimalsCells
    30. Saha K, Sontheimer EJ, Brooks PJ, Dwinell MR, Gersbach CA, Liu DR, Murray SA, Tsai SQ, Wilson RC, Anderson DG, Asokan A, Banfield JF, Bankiewicz KS, Bao G, Bulte JWM, Bursac N, Campbell JM, Carlson DF, Chaikof EL, Chen ZY, Cheng RH, Clark KJ, Curiel DT, Dahlman JE, Deverman BE, Dickinson ME, Doudna JA, Ekker SC, Emborg ME, Feng G, Freedman BS, Gamm DM, Gao G, Ghiran IC, Glazer PM, Gong S, Heaney JD, Hennebold JD, Hinson JT, Khvorova A, Kiani S, Lagor WR, Lam KS, Leong KW, Levine JE, Lewis JA, Lutz CM, Ly DH, Maragh S, McCray PB, McDevitt TC, Mirochnitchenko O, Morizane R, Murthy N, Prather RS, Ronald JA, Roy S, Roy S, Sabbisetti V, Saltzman WM, Santangelo PJ, Segal DJ, Shimoyama M, Skala MC, Tarantal AF, Tilton JC, Truskey GA, Vandsburger M, Watts JK, Wells KD, Wolfe SA, Xu Q, Xue W, Yi G, Zhou J. The NIH Somatic Cell Genome Editing program. Nature. 2021 04; 592(7853):195-204. PMID: 33828315.
      Citations: 45     Fields:    Translation:HumansAnimalsCells
    31. Smith JL, Rodr?guez TC, Mou H, Kwan SY, Pratt H, Zhang XO, Cao Y, Liang S, Ozata DM, Yu T, Yin Q, Hazeltine M, Weng Z, Sontheimer EJ, Xue W. YAP1 Withdrawal in Hepatoblastoma Drives Therapeutic Differentiation of Tumor Cells to Functional Hepatocyte-Like Cells. Hepatology. 2021 03; 73(3):1011-1027. PMID: 32452550.
      Citations: 9     Fields:    Translation:HumansAnimalsCells
    32. Chatterjee P, Jakimo N, Lee J, Amrani N, Rodr?guez T, Koseki SRT, Tysinger E, Qing R, Hao S, Sontheimer EJ, Jacobson J. Publisher Correction: An engineered ScCas9 with broad PAM range and high specificity and activity. Nat Biotechnol. 2020 Oct; 38(10):1212. PMID: 32561876.
      Citations: 1     Fields:    
    33. Barrangou R, Sontheimer EJ. Shutting down RNA-targeting CRISPR. Science. 2020 07 03; 369(6499):31-32. PMID: 32631881.
      Citations:    Fields:    Translation:Cells
    34. Chatterjee P, Lee J, Nip L, Koseki SRT, Tysinger E, Sontheimer EJ, Jacobson JM, Jakimo N. A Cas9 with PAM recognition for adenine dinucleotides. Nat Commun. 2020 05 18; 11(1):2474. PMID: 32424114.
      Citations: 50     Fields:    Translation:HumansCells
    35. Chatterjee P, Jakimo N, Lee J, Amrani N, Rodr?guez T, Koseki SRT, Tysinger E, Qing R, Hao S, Sontheimer EJ, Jacobson J. An engineered ScCas9 with broad PAM range and high specificity and activity. Nat Biotechnol. 2020 10; 38(10):1154-1158. PMID: 32393822.
      Citations: 46     Fields:    Translation:Cells
    36. Davidson AR, Lu WT, Stanley SY, Wang J, Mejdani M, Trost CN, Hicks BT, Lee J, Sontheimer EJ. Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems. Annu Rev Biochem. 2020 Jun 20; 89:309-332. PMID: 32186918.
      Citations: 56     Fields:    Translation:HumansCells
    37. Barrangou R, Sontheimer EJ. CRISPR Shields: Fending Off Diverse Cas Nucleases with Nucleus-like Structures. Mol Cell. 2020 03 05; 77(5):934-936. PMID: 32142691.
      Citations:    Fields:    Translation:Cells
    38. Garcia B, Lee J, Edraki A, Hidalgo-Reyes Y, Erwood S, Mir A, Trost CN, Seroussi U, Stanley SY, Cohn RD, Claycomb JM, Sontheimer EJ, Maxwell KL, Davidson AR. Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing. Cell Rep. 2019 11 12; 29(7):1739-1746.e5. PMID: 31722192.
      Citations: 16     Fields:    Translation:HumansCells
    39. Sun W, Yang J, Cheng Z, Amrani N, Liu C, Wang K, Ibraheim R, Edraki A, Huang X, Wang M, Wang J, Liu L, Sheng G, Yang Y, Lou J, Sontheimer EJ, Wang Y. Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. Mol Cell. 2019 Dec 19; 76(6):938-952.e5. PMID: 31668930.
      Citations: 44     Fields:    Translation:Cells
    40. Lee J, Mou H, Ibraheim R, Liang SQ, Liu P, Xue W, Sontheimer EJ. Tissue-restricted genome editing in vivo specified by microRNA-repressible anti-CRISPR proteins. RNA. 2019 Nov; 25(11):1421-1431. PMID: 31439808.
      Citations: 35     Fields:    Translation:HumansAnimalsCells
    41. Thavalingam A, Cheng Z, Garcia B, Huang X, Shah M, Sun W, Wang M, Harrington L, Hwang S, Hidalgo-Reyes Y, Sontheimer EJ, Doudna J, Davidson AR, Moraes TF, Wang Y, Maxwell KL. Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2. Nat Commun. 2019 06 26; 10(1):2806. PMID: 31243272.
      Citations: 27     Fields:    Translation:Cells
    42. Sontheimer EJ. X-Tracting a New CRISPR-Cas Genome-Editing Platform from Metagenomic Data Sets. CRISPR J. 2019 06; 2:148-150. PMID: 31225753.
      Citations:    Fields:    Translation:Cells
    43. Edraki A, Mir A, Ibraheim R, Gainetdinov I, Yoon Y, Song CQ, Cao Y, Gallant J, Xue W, Rivera-P?rez JA, Sontheimer EJ. A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In?Vivo Genome Editing. Mol Cell. 2019 Feb 21; 73(4):714-726.e4. PMID: 30581144.
      Citations: 113     Fields:    Translation:HumansAnimalsCells
    44. Gao XD, Rodr?guez TC, Sontheimer EJ. Adapting dCas9-APEX2 for subnuclear proteomic profiling. Methods Enzymol. 2019; 616:365-383. PMID: 30691651.
      Citations: 3     Fields:    Translation:HumansCells
    45. Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA. Publisher Correction: Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Nat Commun. 2018 12 10; 9(1):5294. PMID: 30531933.
      Citations:    Fields:    
    46. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ. NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol. 2018 12 05; 19(1):214. PMID: 30518407.
      Citations: 54     Fields:    Translation:HumansAnimalsCells
    47. Lee J, Mir A, Edraki A, Garcia B, Amrani N, Lou HE, Gainetdinov I, Pawluk A, Ibraheim R, Gao XD, Liu P, Davidson AR, Maxwell KL, Sontheimer EJ. Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins. mBio. 2018 12 04; 9(6). PMID: 30514786.
      Citations: 43     Fields:    Translation:HumansCells
    48. Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA. Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Nat Commun. 2018 11 19; 9(1):4856. PMID: 30451839.
      Citations: 19     Fields:    Translation:HumansCells
    49. Ibraheim R, Song CQ, Mir A, Amrani N, Xue W, Sontheimer EJ. All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo. Genome Biol. 2018 09 19; 19(1):137. PMID: 30231914.
      Citations: 52     Fields:    Translation:AnimalsCells
    50. Mir A, Alterman JF, Hassler MR, Debacker AJ, Hudgens E, Echeverria D, Brodsky MH, Khvorova A, Watts JK, Sontheimer EJ. Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing. Nat Commun. 2018 Jul 06; 9(1):2641. PMID: 29980686.
      Citations: 36     Fields:    Translation:HumansCells
    51. Stone NP, Hilbert BJ, Hidalgo D, Halloran KT, Lee J, Sontheimer EJ, Kelch BA. A Hyperthermophilic Phage Decoration Protein Suggests Common Evolutionary Origin with Herpesvirus Triplex Proteins and an Anti-CRISPR Protein. Structure. 2018 07 03; 26(7):936-947.e3. PMID: 29779790.
      Citations: 12     Fields:    Translation:Cells
    52. Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ. C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2. Nat Methods. 2018 06; 15(6):433-436. PMID: 29735996.
      Citations: 60     Fields:    Translation:HumansCells
    53. Edraki A, Sontheimer EJ. CRISPRs from scratch. Nat Microbiol. 2018 03; 3(3):261-262. PMID: 29463924.
      Citations:    Fields:    Translation:Cells
    54. Mir A, Edraki A, Lee J, Sontheimer EJ. Type II-C CRISPR-Cas9 Biology, Mechanism, and Application. ACS Chem Biol. 2018 Feb 16; 13(2):357-365. PMID: 29202216.
      Citations: 53     Fields:    Translation:Cells
    55. Harrington LB, Doxzen KW, Ma E, Liu JJ, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA. A Broad-Spectrum Inhibitor of CRISPR-Cas9. Cell. 2017 Sep 07; 170(6):1224-1233.e15. PMID: 28844692.
      Citations: 111     Fields:    Translation:HumansCells
    56. Sontheimer EJ, Davidson AR. Inhibition of CRISPR-Cas systems by mobile genetic elements. Curr Opin Microbiol. 2017 Jun; 37:120-127. PMID: 28668720.
      Citations: 18     Fields:    Translation:Cells
    57. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017 Jun 14; 18(1):108. PMID: 28615073.
      Citations: 84     Fields:    Translation:HumansCells
    58. Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. Naturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15; 167(7):1829-1838.e9. PMID: 27984730.
      Citations: 183     Fields:    Translation:HumansCells
    59. Sontheimer EJ, Marraffini LA. RNA. CRISPR goes retro. Science. 2016 Feb 26; 351(6276):920-1. PMID: 26917756.
      Citations:    Fields:    Translation:Cells
    60. Sontheimer EJ, Wolfe SA. Cas9 gets a classmate. Nat Biotechnol. 2015 Dec 09; 33(12):1240-1241. PMID: 26650011.
      Citations: 2     Fields:    
    61. Zhang Y, Rajan R, Seifert HS, Mondrag?n A, Sontheimer EJ. DNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15; 60(2):242-55. PMID: 26474066.
      Citations: 33     Fields:    Translation:Cells
    62. Wakefield N, Rajan R, Sontheimer EJ. Primary processing of CRISPR RNA by the endonuclease Cas6 in Staphylococcus epidermidis. FEBS Lett. 2015 Oct 07; 589(20 Pt B):3197-204. PMID: 26364721.
      Citations: 4     Fields:    Translation:Cells
    63. Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ, Weng Z, Gao G, Xue W. Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Hum Gene Ther. 2015 Jul; 26(7):432-42. PMID: 26086867.
      Citations: 160     Fields:    Translation:HumansAnimalsCells
    64. Sontheimer EJ, Barrangou R. The Bacterial Origins of the CRISPR Genome-Editing Revolution. Hum Gene Ther. 2015 Jul; 26(7):413-24. PMID: 26078042.
      Citations: 33     Fields:    Translation:HumansAnimalsCells
    65. Sontheimer E. Accelerating expansion. RNA. 2015 Apr; 21(4):510. PMID: 25780117.
      Citations:    Fields:    
    66. Zhang Y, Sontheimer EJ. Structural biology. Cascading into focus. Science. 2014 Sep 19; 345(6203):1452-3. PMID: 25237089.
      Citations: 2     Fields:    Translation:Cells
    67. Hurtado S, Kim Guisbert KS, Sontheimer EJ. SPO24 is a transcriptionally dynamic, small ORF-encoding locus required for efficient sporulation in Saccharomyces cerevisiae. PLoS One. 2014; 9(8):e105058. PMID: 25127041.
      Citations: 6     Fields:    Translation:AnimalsCells
    68. Doudna JA, Sontheimer EJ. Methods in Enzymology. The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations. Preface. Methods Enzymol. 2014; 546:xix-xx. PMID: 25398356.
      Citations: 19     Fields:    Translation:HumansAnimalsCells
    69. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Sep 24; 110(39):15644-9. PMID: 23940360.
      Citations: 347     Fields:    Translation:HumansAnimalsCells
    70. Zhang Y, Heidrich N, Ampattu BJ, Gunderson CW, Seifert HS, Schoen C, Vogel J, Sontheimer EJ. Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis. Mol Cell. 2013 May 23; 50(4):488-503. PMID: 23706818.
      Citations: 140     Fields:    Translation:HumansCells
    71. Kim Guisbert KS, Sontheimer EJ. Quit stalling or you'll be silenced. Cell. 2013 Feb 28; 152(5):938-9. PMID: 23452843.
      Citations: 1     Fields:    
    72. Sontheimer EJ. Small RNAs of opposite sign? but same absolute value. Cell. 2012 Dec 07; 151(6):1157-8. PMID: 23217700.
      Citations:    Fields:    
    73. Kim Guisbert KS, Zhang Y, Flatow J, Hurtado S, Staley JP, Lin S, Sontheimer EJ. Meiosis-induced alterations in transcript architecture and noncoding RNA expression in S. cerevisiae. RNA. 2012 Jun; 18(6):1142-53. PMID: 22539527.
      Citations: 14     Fields:    Translation:AnimalsCells
    74. Gerbasi VR, Preall JB, Golden DE, Powell DW, Cummins TD, Sontheimer EJ. Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis. Proc Natl Acad Sci U S A. 2011 Feb 22; 108(8):3204-9. PMID: 21300896.
      Citations: 17     Fields:    Translation:AnimalsCells
    75. Sontheimer EJ, Marraffini LA. Microbiology: slicer for DNA. Nature. 2010 Nov 04; 468(7320):45-6. PMID: 21048757.
      Citations: 8     Fields:    Translation:Cells
    76. Gerbasi VR, Golden DE, Hurtado SB, Sontheimer EJ. Proteomics identification of Drosophila small interfering RNA-associated factors. Mol Cell Proteomics. 2010 Sep; 9(9):1866-72. PMID: 20472918.
      Citations: 5     Fields:    Translation:AnimalsCells
    77. Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet. 2010 Mar; 11(3):181-90. PMID: 20125085.
      Citations: 428     Fields:    Translation:Cells
    78. Marraffini LA, Sontheimer EJ. Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature. 2010 Jan 28; 463(7280):568-71. PMID: 20072129.
      Citations: 301     Fields:    Translation:Cells
    79. Lee YS, Pressman S, Andress AP, Kim K, White JL, Cassidy JJ, Li X, Lubell K, Lim DH, Cho IS, Nakahara K, Preall JB, Bellare P, Sontheimer EJ, Carthew RW. Silencing by small RNAs is linked to endosomal trafficking. Nat Cell Biol. 2009 Sep; 11(9):1150-6. PMID: 19684574.
      Citations: 184     Fields:    Translation:HumansAnimalsCells
    80. Marraffini LA, Sontheimer EJ. Invasive DNA, chopped and in the CRISPR. Structure. 2009 Jun 10; 17(6):786-8. PMID: 19523896.
      Citations: 13     Fields:    Translation:Cells
    81. Carthew RW, Sontheimer EJ. Origins and Mechanisms of miRNAs and siRNAs. Cell. 2009 Feb 20; 136(4):642-55. PMID: 19239886.
      Citations: 2133     Fields:    Translation:HumansAnimals
    82. Marraffini LA, Sontheimer EJ. CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA. Science. 2008 Dec 19; 322(5909):1843-5. PMID: 19095942.
      Citations: 746     Fields:    Translation:Cells
    83. Golden DE, Gerbasi VR, Sontheimer EJ. An inside job for siRNAs. Mol Cell. 2008 Aug 08; 31(3):309-12. PMID: 18691963.
      Citations: 48     Fields:    Translation:AnimalsCells
    84. Bellare P, Small EC, Huang X, Wohlschlegel JA, Staley JP, Sontheimer EJ. A role for ubiquitin in the spliceosome assembly pathway. Nat Struct Mol Biol. 2008 May; 15(5):444-51. PMID: 18425143.
      Citations: 73     Fields:    Translation:AnimalsCells
    85. Bellare P, Sontheimer EJ. A fork in the road for microRNAs. Nat Struct Mol Biol. 2007 Aug; 14(8):684-6. PMID: 17676029.
      Citations: 1     Fields:    Translation:Animals
    86. Preall JB, He Z, Gorra JM, Sontheimer EJ. Short interfering RNA strand selection is independent of dsRNA processing polarity during RNAi in Drosophila. Curr Biol. 2006 Mar 07; 16(5):530-5. PMID: 16527750.
      Citations: 19     Fields:    Translation:AnimalsCells
    87. Bellare P, Kutach AK, Rines AK, Guthrie C, Sontheimer EJ. Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. 2006 Feb; 12(2):292-302. PMID: 16428608.
      Citations: 49     Fields:    Translation:Cells
    88. Preall JB, Sontheimer EJ. RNAi: RISC gets loaded. Cell. 2005 Nov 18; 123(4):543-5. PMID: 16286001.
      Citations: 43     Fields:    Translation:HumansAnimalsCells
    89. Pham JW, Sontheimer EJ. Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway. J Biol Chem. 2005 Nov 25; 280(47):39278-83. PMID: 16179342.
      Citations: 34     Fields:    Translation:AnimalsCells
    90. Pellino JL, Jaskiewicz L, Filipowicz W, Sontheimer EJ. ATP modulates siRNA interactions with an endogenous human Dicer complex. RNA. 2005 Nov; 11(11):1719-24. PMID: 16177131.
      Citations: 11     Fields:    Translation:HumansCells
    91. Sontheimer EJ, Carthew RW. Silence from within: endogenous siRNAs and miRNAs. Cell. 2005 Jul 15; 122(1):9-12. PMID: 16009127.
      Citations: 97     Fields:    Translation:HumansAnimalsCells
    92. Sontheimer EJ. Assembly and function of RNA silencing complexes. Nat Rev Mol Cell Biol. 2005 Feb; 6(2):127-38. PMID: 15654322.
      Citations: 141     Fields:    Translation:HumansAnimalsCells
    93. Pham JW, Sontheimer EJ. Separation of Drosophila RNA silencing complexes by native gel electrophoresis. Methods Mol Biol. 2005; 309:11-6. PMID: 15990394.
      Citations: 1     Fields:    Translation:Animals
    94. Sontheimer EJ, Carthew RW. Molecular biology. Argonaute journeys into the heart of RISC. Science. 2004 Sep 03; 305(5689):1409-10. PMID: 15353786.
      Citations: 18     Fields:    Translation:HumansAnimalsCells
    95. He Z, Sontheimer EJ. "siRNAs and miRNAs": a meeting report on RNA silencing. RNA. 2004 Aug; 10(8):1165-73. PMID: 15272116.
      Citations: 4     Fields:    Translation:HumansAnimals
    96. Pham JW, Sontheimer EJ. The Making of an siRNA. Mol Cell. 2004 Jul 23; 15(2):163-4. PMID: 15260964.
      Citations: 2     Fields:    Translation:HumansAnimalsCells
    97. Pham JW, Radhakrishnan I, Sontheimer EJ. Thermodynamic and structural characterization of 2'-nitrogen-modified RNA duplexes. Nucleic Acids Res. 2004; 32(11):3446-55. PMID: 15247335.
      Citations: 1     Fields:    Translation:Cells
    98. Pham JW, Pellino JL, Lee YS, Carthew RW, Sontheimer EJ. A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell. 2004 Apr 02; 117(1):83-94. PMID: 15066284.
      Citations: 166     Fields:    Translation:AnimalsCells
    99. Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ, Carthew RW. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell. 2004 Apr 02; 117(1):69-81. PMID: 15066283.
      Citations: 565     Fields:    Translation:AnimalsCells
    100. Pellino JL, Sontheimer EJ. R2D2 leads the silencing trigger to mRNA's death star. Cell. 2003 Oct 17; 115(2):132-3. PMID: 14567910.
      Citations: 3     Fields:    Translation:AnimalsCells
    101. Sontheimer EJ. The spliceosome shows its metal. Nat Struct Biol. 2001 Jan; 8(1):11-3. PMID: 11135658.
      Citations: 3     Fields:    Translation:AnimalsCells
    102. Gordon PM, Sontheimer EJ, Piccirilli JA. Kinetic characterization of the second step of group II intron splicing: role of metal ions and the cleavage site 2'-OH in catalysis. Biochemistry. 2000 Oct 24; 39(42):12939-52. PMID: 11041859.
      Citations: 13     Fields:    Translation:Cells
    103. Warnecke JM, Sontheimer EJ, Piccirilli JA, Hartmann RK. Active site constraints in the hydrolysis reaction catalyzed by bacterial RNase P: analysis of precursor tRNAs with a single 3'-S-phosphorothiolate internucleotide linkage. Nucleic Acids Res. 2000 Feb 01; 28(3):720-7. PMID: 10637323.
      Citations: 14     Fields:    Translation:Cells
    104. Gordon PM, Sontheimer EJ, Piccirilli JA. Metal ion catalysis during the exon-ligation step of nuclear pre-mRNA splicing: extending the parallels between the spliceosome and group II introns. RNA. 2000 Feb; 6(2):199-205. PMID: 10688359.
      Citations: 46     Fields:    Translation:HumansCells
    105. Sontheimer EJ, Gordon PM, Piccirilli JA. Metal ion catalysis during group II intron self-splicing: parallels with the spliceosome. Genes Dev. 1999 Jul 01; 13(13):1729-41. PMID: 10398685.
      Citations: 46     Fields:    Translation:AnimalsCells
    106. Sontheimer EJ. Bridging sulfur substitutions in the analysis of pre-mRNA splicing. Methods. 1999 May; 18(1):29-37. PMID: 10208814.
      Citations: 3     Fields:    Translation:Cells
    107. Sontheimer EJ, Sun S, Piccirilli JA. Metal ion catalysis during splicing of premessenger RNA. Nature. 1997 Aug 21; 388(6644):801-5. PMID: 9285595.
      Citations: 57     Fields:    Translation:HumansCells
    108. Sontheimer EJ. Site-specific RNA crosslinking with 4-thiouridine. Mol Biol Rep. 1994 Jul; 20(1):35-44. PMID: 7531281.
      Citations: 27     Fields:    Translation:Cells
    109. Sontheimer EJ, Steitz JA. The U5 and U6 small nuclear RNAs as active site components of the spliceosome. Science. 1993 Dec 24; 262(5142):1989-96. PMID: 8266094.
      Citations: 162     Fields:    Translation:Cells
    110. Cortes JJ, Sontheimer EJ, Seiwert SD, Steitz JA. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo. EMBO J. 1993 Dec 15; 12(13):5181-9. PMID: 8262061.
      Citations: 39     Fields:    Translation:HumansAnimalsCells
    111. Wyatt JR, Sontheimer EJ, Steitz JA. Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. Genes Dev. 1992 Dec; 6(12B):2542-53. PMID: 1340469.
      Citations: 131     Fields:    Translation:HumansCells
    112. Gelpi C, Sontheimer EJ, Rodriguez-Sanchez JL. Autoantibodies against a serine tRNA-protein complex implicated in cotranslational selenocysteine insertion. Proc Natl Acad Sci U S A. 1992 Oct 15; 89(20):9739-43. PMID: 1409691.
      Citations: 39     Fields:    Translation:HumansCells
    113. Sontheimer EJ, Steitz JA. Three novel functional variants of human U5 small nuclear RNA. Mol Cell Biol. 1992 Feb; 12(2):734-46. PMID: 1310151.
      Citations: 21     Fields:    Translation:HumansCells
    Sontheimer's Networks
    Click the
    buttons for more information and interactive visualizations!
    Concepts (341)
    Co-Authors (44)
    Similar People (60)
    Same Department Expand Description
    Physical Neighbors