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Melissa J Moore PhD

TitleProfessor
InstitutionUMass Chan Medical School
DepartmentRNA Therapeutics Institute
AddressUMass Chan Medical School
364 Plantation Street LRB-825
Worcester MA 01605
Phone508-856-8014
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentRNA Therapeutics Institute

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBioinformatics and Computational Biology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentNeuroscience

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentTranslational Science

    InstitutionUMass Chan Programs, Centers and Institutes
    DepartmentBioinformatics and Integrative Biology

    InstitutionUMass Chan Programs, Centers and Institutes
    DepartmentChemical Biology


    Collapse Biography 
    Collapse education and training
    College of William and Mary, Williamsburg, VA, United StatesBSChemistry/ Biology
    Massachusetts Institute of Technology, Cambridge, MA, United StatesPHDBiological Chemistry

    Collapse Overview 
    Collapse overview

    Eleanor Eustis Farrington Chair of Cancer Research
    Professor, RNA Therapeutics Institutes (RTI)


    Eukaryotic RNA Processing and Metabolism


    melissa moore


    Melissa Moore’s work encompasses a broad array of topics involved in post-transcriptional gene regulation in eukaryotes via mechanisms involving RNA.

    Our research currently focuses on three distinct but interconnected areas involving the basic mechanisms of eukaryotic gene expression: (1) the structure and mechanism of the spliceosome, (2) the effects of nuclear-acquired proteins on cytoplasmic messenger RNA (mRNA) metabolism, and (3) the fate of functionally defective ribosomal RNAs (rRNAs) and mRNAs.

    Introns are incoherent strings of nucleotides that interrupt the coding regions of genes. They are removed from nascent RNA transcripts by the process of precursor mRNA (pre- mRNA) splicing.

    Since the majority of genes in multicellular organisms contain introns, their timely and precise removal is essential for proper gene expression. Most introns are excised by the major spliceosome, a complex macromolecular machine containing five stable, small nuclear RNAs (snRNAs) and a multitude of proteins. The spliceosome must be at once precise (e.g., a 1-nucleotide shift in a splice site will throw the protein-coding region completely out of frame) and adaptable (in humans it must recognize >10^5 different splice site pairs in diverse sequence contexts). In metazoans, the recognition problem is compounded by poor conservation of the sequences defining splice sites and the presence of multiple introns per pre-mRNA. Also, a remarkably high percentage of metazoan pre-mRNAs are subject to alternative splicing, which greatly expands the repertoire of proteins that can be expressed from relatively small genomes.

    A major goal of our research is to elucidate the basic mechanisms by which mammalian spliceosomes accurately identify splice sites in pre-mRNAs and then catalyze intron excision. For some time, our primary focus has been the second step of splicing, wherein the intron is excised and the expressed regions (or exons) are ligated together. Recently we succeeded in purifying, in their native state, spliceosomes poised to perform this reaction. Mass spectrometry revealed more than 100 polypeptides associated with this structure. Using techniques for single-particle image reconstruction from electron micrographs, we obtained an initial three-dimensional structural map to ~30-Å resolution. The structure, with dimensions ~240 x 270 Å, exhibits three major domains connected via a series of bridges and tunnels. Further structural analysis is under way. (This work has been carried out in collaboration with Nikolaus Grigorieff [HHMI, Brandeis University].)




    On the mechanistic front, we have recently developed methodologies for following pre- mRNA splicing at the single-molecule level. All previous in vitro mechanistic studies of splicing have utilized ensemble assays that report only the average behavior of a population. Although such bulk assays have provided a wealth of mechanistic insight, they are ultimately limited in their ability to tease out finer mechanistic details. Over the past two decades, single-molecule techniques that complement ensemble measurements have emerged as powerful tools to elucidate the enzyme mechanism. These approaches permit observation of the stochastic behavior of individual binding and catalytic events. They also allow observation of many individual events that would otherwise go undetected.


    Using a pre-mRNA attached to a glass surface via end and containing fluorescent labels in the 5' exon and intron, we are now able to observe individual splicing events in Saccharomyces cerevisiae extracts, using a multiwavelength total internal reflection fluorescence (TIRF) microscope system developed by Jeff Gelles (Brandeis University) and his colleagues. Chemical biology tools are being used to fluorescently label core spliceosomal proteins and snRNAs, as well as a number of transiently associating splicing factors. This system allows us to analyze the dynamic characteristics of individual spliceosomes in real time. It should provide a new window into previously unaddressable questions regarding spliceosome assembly and internal structural transitions, as well as the comings and goings of key splicing factors. (All of the spliceosome structure and mechanism work is supported by a grant from the National Institutes of Health.)


    Structure and Assembly of the Exon Junction Complex


    In addition to removing introns, the process of pre-mRNA splicing has significant consequences for the subsequent metabolism of the product mRNA. That is, mRNAs produced by splicing are subject to different subcellular localization, different


    efficiencies of translation into proteins, and different decay rates than otherwise identical mRNAs produced from intronless genes. Splicing affects downstream mRNA metabolism by altering the complement of proteins that associate with the mRNA to form an mRNP (mRNA ribonucleoprotein particle). Several years ago, in collaboration with Lynne Maquat (University of Rochester) and Elisa Izaurralde (European Molecular Biology Laboratory, Heidelberg), we showed that spliceosomes stably deposit a complex of proteins (the EJC) on mRNAs at a conserved position 20–24 nucleotides upstream of exon-exon junctions. Such EJCs accompany spliced mRNAs to the cytoplasm, where they are ultimately displaced by the process of translation.


    A major unresolved question regarding the EJC had been how this complex manages to bind so tightly to a specific position on mRNA in what seems to be an entirely RNA structure- and sequence-independent fashion. We solved this mystery by identifying eIF4AIII as the EJC anchor. A member of the DEAD-box family of RNA helicases, eIF4AIII represents a new functional class of such proteins that act as RNA "placeholders" or "clothespins" rather than RNA translocases. Such place-holding DEAD-box proteins could serve as a general means for attaching factors that add functionality to an RNP without requiring any special consensus sequences in the RNA.


    Functional Consequences of EJC Deposition


    As stated above, spliced mRNAs exhibit metabolic fates different from the metabolic fates of mRNAs not produced by splicing. We have been investigating to what extent and by what mechanism(s) EJC deposition contributes to these differences. One area of investigation is the efficiency by which mRNAs are utilized as templates for making proteins. Quantitative analysis revealed that two to three times as many protein molecules are made per spliced mRNA molecule than per identical mRNA molecules not made by splicing. Polysome analysis revealed that spliced mRNAs interact more efficiently with ribosomes, the macromolecular machines that use mRNAs as the blueprints to synthesize proteins, than do unspliced mRNAs. This effect may facilitate the rapid expression of newly made mRNAs by enabling them to outcompete translationally experienced mRNAs (that no longer carry EJCs) for limiting translation initiation factors.


    Recently, in collaboration with Gina Turrigiano (Brandeis University) and Christopher Burge (Massachusetts Institute of Technology), we found that eIF4AIII remains associated with dendritically localized mRNAs in mammalian neurons. eIF4AIII knockdown up-regulates at least two proteins involved in postsynaptic function and markedly increases synaptic strength. Thus eIF4AIII appears to act as a key brake on expression of proteins required for synaptic function. One mechanism for this braking action is via the translation-dependent decay of Arc mRNA, the gene for which contains two conserved introns in its 3'-untranslated region (3'-UTR). This is a highly unusual gene structure in mammals, as EJCs downstream of stop codons trigger nonsense- mediated mRNA decay (NMD). A bioinformatics approach revealed 148 other mammalian genes with this same feature, suggesting that translation-dependent mRNA decay mechanisms such as NMD might be widely employed in mammalian cells as a means to limit the amount of protein produced from certain mRNAs. Curiously, a large number of these genes are expressed in hematopoietic cells, suggesting that some feature of blood cells may particularly favor their evolution there. Future experiments will probe the role of the EJC in modulating expression from some of these new 3'-UTR intron- containing genes.


    Clearance of Nonfunctional Ribosomes


    The ribosome is the most abundant macromolecular machine in the cell. Its highly complex structure, composed of both ribosomal RNAs (rRNAs) and proteins, necessitates an intricate assembly mechanism in which pre-rRNA processing and nucleotide modification are coupled with chaperone-assisted rRNA folding and protein association.


    Although the mechanics of this assembly process are becoming increasingly understood, surprisingly little is known about the mechanisms assuring its overall fidelity. Furthermore, given their inordinately long half-lives in eukaryotic cells, it is to be expected that some ribosomes will become nonfunctional over time as they accumulate oxidative damage due to normal cellular metabolism. We therefore wondered whether eukaryotes might possess any mechanisms for eliminating ribosomes that are fully assembled but functionally defective, akin to their abilities to eliminate mRNAs that are fully processed but defective. To test this, we introduced point mutations into the peptidyltransferase center of 25S rRNA and the decoding center of 18S rRNA in S. cerevisiae. These mutant rRNAs are assembled into ribosomes, but they display markedly decreased steady-state levels compared to wild-type rRNAs.


    Preliminary analyses of knockout strains have revealed several candidate genes important for decreased expression of the translationally defective mutant rRNAs. Our results therefore indicate that budding yeast do contain a quality control system capable of recognizing and eliminating translationally deficient ribosomes so as to prevent their interference with normal cellular function. We continue to study the trans-acting factors and molecular mechanisms involved in this process.



    Collapse Rotation Projects

    Our laboratory combines biochemical, biophysical, molecular and cell biological approaches to investigate various aspects of pre-mRNA processing, mRNA metabolism and RNA quality control in eukaryotic cells. Potential rotation projects are available in all areas of the laboratory's interests (see Research section).



    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Davis SM, Hariharan VN, Lo A, Turanov AA, Echeverria D, Sousa J, McHugh N, Biscans A, Alterman JF, Karumanchi SA, Moore MJ, Khvorova A. Chemical optimization of siRNA for safe and efficient silencing of placental sFLT1. Mol Ther Nucleic Acids. 2022 Sep 13; 29:135-149. PMID: 35847173.
      Citations:    
    2. Kovalak C, Donovan S, Bicknell AA, Metkar M, Moore MJ. Deep sequencing of pre-translational mRNPs reveals hidden flux through evolutionarily conserved alternative splicing nonsense-mediated decay pathways. Genome Biol. 2021 05 03; 22(1):132. PMID: 33941243.
      Citations: 4     Fields:    Translation:HumansCells
    3. Mauger DM, Cabral BJ, Presnyak V, Su SV, Reid DW, Goodman B, Link K, Khatwani N, Reynders J, Moore MJ, McFadyen IJ. mRNA structure regulates protein expression through changes in functional half-life. Proc Natl Acad Sci U S A. 2019 11 26; 116(48):24075-24083. PMID: 31712433.
      Citations: 86     Fields:    Translation:HumansCells
    4. Saini H, Bicknell AA, Eddy SR, Moore MJ. Free circular introns with an unusual branchpoint in neuronal projections. Elife. 2019 11 07; 8. PMID: 31697236.
      Citations: 5     Fields:    Translation:AnimalsCells
    5. Hassett KJ, Benenato KE, Jacquinet E, Lee A, Woods A, Yuzhakov O, Himansu S, Deterling J, Geilich BM, Ketova T, Mihai C, Lynn A, McFadyen I, Moore MJ, Senn JJ, Stanton MG, Almarsson Ö, Ciaramella G, Brito LA. Optimization of Lipid Nanoparticles for Intramuscular Administration of mRNA Vaccines. Mol Ther Nucleic Acids. 2019 Apr 15; 15:1-11. PMID: 30785039.
      Citations:    
    6. Kucukural A, Yukselen O, Ozata DM, Moore MJ, Garber M. DEBrowser: interactive differential expression analysis and visualization tool for count data. BMC Genomics. 2019 Jan 05; 20(1):6. PMID: 30611200.
      Citations: 67     Fields:    Translation:HumansCells
    7. Turanov AA, Lo A, Hassler MR, Makris A, Ashar-Patel A, Alterman JF, Coles AH, Haraszti RA, Roux L, Godinho BMDC, Echeverria D, Pears S, Iliopoulos J, Shanmugalingam R, Ogle R, Zsengeller ZK, Hennessy A, Karumanchi SA, Moore MJ, Khvorova A. RNAi modulation of placental sFLT1 for the treatment of preeclampsia. Nat Biotechnol. 2018 Nov 19. PMID: 30451990.
      Citations: 38     Fields:    
    8. Metkar M, Ozadam H, Lajoie BR, Imakaev M, Mirny LA, Dekker J, Moore MJ. Higher-Order Organization Principles of Pre-translational mRNPs. Mol Cell. 2018 11 15; 72(4):715-726.e3. PMID: 30415953.
      Citations: 22     Fields:    Translation:HumansCells
    9. Didiot MC, Ferguson CM, Ly S, Coles AH, Smith AO, Bicknell AA, Hall LM, Sapp E, Echeverria D, Pai AA, DiFiglia M, Moore MJ, Hayward LJ, Aronin N, Khvorova A. Nuclear Localization of Huntingtin mRNA Is Specific to Cells of Neuronal Origin. Cell Rep. 2018 09 04; 24(10):2553-2560.e5. PMID: 30184490.
      Citations: 10     Fields:    Translation:HumansAnimalsCells
    10. Jain R, Frederick JP, Huang EY, Burke KE, Mauger DM, Andrianova EA, Farlow SJ, Siddiqui S, Pimentel J, Cheung-Ong K, McKinney KM, Köhrer C, Moore MJ, Chakraborty T. MicroRNAs Enable mRNA Therapeutics to Selectively Program Cancer Cells to Self-Destruct. Nucleic Acid Ther. 2018 10; 28(5):285-296. PMID: 30088967.
      Citations: 25     Fields:    Translation:HumansAnimalsCells
    11. Braun JE, Friedman LJ, Gelles J, Moore MJ. Synergistic assembly of human pre-spliceosomes across introns and exons. Elife. 2018 06 22; 7. PMID: 29932423.
      Citations: 4     Fields:    Translation:HumansCells
    12. Noma A, Smith CS, Huisman M, Martin RM, Moore MJ, Grunwald D. Advanced 3D Analysis and Optimization of Single-Molecule FISH in Drosophila Muscle. Small Methods. 2018 Sep 11; 2(9). PMID: 32158910.
      Citations:    Fields:    
    13. Chen W, Moore J, Ozadam H, Shulha HP, Rhind N, Weng Z, Moore MJ. Transcriptome-wide Interrogation of the Functional Intronome by Spliceosome Profiling. Cell. 2018 05 03; 173(4):1031-1044.e13. PMID: 29727662.
      Citations: 10     Fields:    Translation:AnimalsCells
    14. Limoncelli KA, Merrikh CN, Moore MJ. Corrigendum: ASC1 and RPS3: new actors in 18S nonfunctional rRNA decay. RNA. 2018 04; 24(4):620. PMID: 29549138.
      Citations:    Fields:    
    15. Hassler MR, Turanov AA, Alterman JF, Haraszti RA, Coles AH, Osborn MF, Echeverria D, Nikan M, Salomon WE, Roux L, Godinho BMDC, Davis SM, Morrissey DV, Zamore PD, Karumanchi SA, Moore MJ, Aronin N, Khvorova A. Comparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo. Nucleic Acids Res. 2018 03 16; 46(5):2185-2196. PMID: 29432571.
      Citations: 49     Fields:    Translation:HumansAnimalsCells
    16. Limoncelli KA, Merrikh CN, Moore MJ. ASC1 and RPS3: new actors in 18S nonfunctional rRNA decay. RNA. 2017 12; 23(12):1946-1960. PMID: 28956756.
      Citations: 16     Fields:    Translation:AnimalsCells
    17. Ashar-Patel A, Kaymaz Y, Rajakumar A, Bailey JA, Karumanchi SA, Moore MJ. FLT1 and transcriptome-wide polyadenylation site (PAS) analysis in preeclampsia. Sci Rep. 2017 09 22; 7(1):12139. PMID: 28939845.
      Citations: 21     Fields:    Translation:HumansCells
    18. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017 06 14; 18(1):108. PMID: 28615073.
      Citations: 74     Fields:    Translation:HumansCells
    19. Cenik C, Chua HN, Singh G, Akef A, Snyder MP, Palazzo AF, Moore MJ, Roth FP. A common class of transcripts with 5'-intron depletion, distinct early coding sequence features, and N1-methyladenosine modification. RNA. 2017 03; 23(3):270-283. PMID: 27994090.
      Citations: 9     Fields:    Translation:HumansCells
    20. Salomon WE, Jolly SM, Moore MJ, Zamore PD, Serebrov V. Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides. Cell. 2016 07 14; 166(2):517-520. PMID: 28777949.
      Citations: 7     Fields:    
    21. Atianand MK, Hu W, Satpathy AT, Shen Y, Ricci EP, Alvarez-Dominguez JR, Bhatta A, Schattgen SA, McGowan JD, Blin J, Braun JE, Gandhi P, Moore MJ, Chang HY, Lodish HF, Caffrey DR, Fitzgerald KA. A Long Noncoding RNA lincRNA-EPS Acts as a Transcriptional Brake to Restrain Inflammation. Cell. 2016 Jun 16; 165(7):1672-1685. PMID: 27315481.
      Citations: 197     Fields:    Translation:HumansAnimalsCells
    22. Hoskins AA, Rodgers ML, Friedman LJ, Gelles J, Moore MJ. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. Elife. 2016 05 31; 5. PMID: 27244240.
      Citations: 22     Fields:    Translation:AnimalsCells
    23. Heyer EE, Moore MJ. Redefining the Translational Status of 80S Monosomes. Cell. 2016 Feb 11; 164(4):757-69. PMID: 26871635.
      Citations: 92     Fields:    Translation:AnimalsCells
    24. Serebrov V, Moore MJ. Single Molecule Approaches in RNA-Protein Interactions. Adv Exp Med Biol. 2016; 907:89-106. PMID: 27256383.
      Citations:    Fields:    Translation:HumansCells
    25. Sharma U, Conine CC, Shea JM, Boskovic A, Derr AG, Bing XY, Belleannee C, Kucukural A, Serra RW, Sun F, Song L, Carone BR, Ricci EP, Li XZ, Fauquier L, Moore MJ, Sullivan R, Mello CC, Garber M, Rando OJ. Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals. Science. 2016 Jan 22; 351(6271):391-396. PMID: 26721685.
      Citations: 440     Fields:    Translation:AnimalsCells
    26. Salomon WE, Jolly SM, Moore MJ, Zamore PD, Serebrov V. Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides. Cell. 2015 Jul 02; 162(1):84-95. PMID: 26140592.
      Citations: 119     Fields:    Translation:AnimalsCells
    27. Roy CK, Olson S, Graveley BR, Zamore PD, Moore MJ. Assessing long-distance RNA sequence connectivity via RNA-templated DNA-DNA ligation. Elife. 2015 Apr 13; 4. PMID: 25866926.
      Citations: 14     Fields:    Translation:HumansAnimalsCells
    28. Moore MJ. Twenty years of technology. RNA. 2015 Apr; 21(4):697-8. PMID: 25780196.
      Citations:    Fields:    Translation:Cells
    29. Singh G, Pratt G, Yeo GW, Moore MJ. The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion. Annu Rev Biochem. 2015; 84:325-54. PMID: 25784054.
      Citations: 161     Fields:    Translation:HumansAnimalsCells
    30. Chen W, Moore MJ. Spliceosomes. Curr Biol. 2015 Mar 02; 25(5):R181-3. PMID: 25734262.
      Citations: 21     Fields:    Translation:HumansCells
    31. Heyer EE, Ozadam H, Ricci EP, Cenik C, Moore MJ. An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments. Nucleic Acids Res. 2015 Jan; 43(1):e2. PMID: 25505164.
      Citations: 27     Fields:    Translation:Cells
    32. Carpenter S, Ricci EP, Mercier BC, Moore MJ, Fitzgerald KA. Post-transcriptional regulation of gene expression in innate immunity. Nat Rev Immunol. 2014 Jun; 14(6):361-76. PMID: 24854588.
      Citations: 145     Fields:    Translation:HumansAnimalsCells
    33. Yang C, Wang H, Qiao T, Yang B, Aliaga L, Qiu L, Tan W, Salameh J, McKenna-Yasek DM, Smith T, Peng L, Moore MJ, Brown RH, Cai H, Xu Z. Partial loss of TDP-43 function causes phenotypes of amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 2014 Mar 25; 111(12):E1121-9. PMID: 24616503.
      Citations: 80     Fields:    Translation:Animals
    34. Chen W, Moore MJ. The spliceosome: disorder and dynamics defined. Curr Opin Struct Biol. 2014 Feb; 24:141-9. PMID: 24530854.
      Citations: 39     Fields:    Translation:HumansAnimalsCells
    35. Chen W, Shulha HP, Ashar-Patel A, Yan J, Green KM, Query CC, Rhind N, Weng Z, Moore MJ. Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes. RNA. 2014 Mar; 20(3):308-20. PMID: 24442611.
      Citations: 19     Fields:    Translation:HumansAnimalsCells
    36. Ricci EP, Kucukural A, Cenik C, Mercier BC, Singh G, Heyer EE, Ashar-Patel A, Peng L, Moore MJ. Staufen1 senses overall transcript secondary structure to regulate translation. Nat Struct Mol Biol. 2014 Jan; 21(1):26-35. PMID: 24336223.
      Citations: 76     Fields:    Translation:HumansCells
    37. Zhang Z, Almeida S, Lu Y, Nishimura AL, Peng L, Sun D, Wu B, Karydas AM, Tartaglia MC, Fong JC, Miller BL, Farese RV, Moore MJ, Shaw CE, Gao FB. Downregulation of microRNA-9 in iPSC-derived neurons of FTD/ALS patients with TDP-43 mutations. PLoS One. 2013; 8(10):e76055. PMID: 24143176.
      Citations: 66     Fields:    Translation:HumansCells
    38. Singh G, Ricci EP, Moore MJ. RIPiT-Seq: a high-throughput approach for footprinting RNA:protein complexes. Methods. 2014 Feb; 65(3):320-32. PMID: 24096052.
      Citations: 39     Fields:    Translation:HumansCells
    39. Shcherbakova I, Hoskins AA, Friedman LJ, Serebrov V, Corrêa IR, Xu MQ, Gelles J, Moore MJ. Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy. Cell Rep. 2013 Oct 17; 5(1):151-65. PMID: 24075986.
      Citations: 37     Fields:    Translation:HumansAnimalsCells
    40. Kucukural A, Özadam H, Singh G, Moore MJ, Cenik C. ASPeak: an abundance sensitive peak detection algorithm for RIP-Seq. Bioinformatics. 2013 Oct 01; 29(19):2485-6. PMID: 23929032.
      Citations: 24     Fields:    
    41. Carpenter S, Aiello D, Atianand MK, Ricci EP, Gandhi P, Hall LL, Byron M, Monks B, Henry-Bezy M, Lawrence JB, O'Neill LA, Moore MJ, Caffrey DR, Fitzgerald KA. A long noncoding RNA mediates both activation and repression of immune response genes. Science. 2013 Aug 16; 341(6147):789-92. PMID: 23907535.
      Citations: 473     Fields:    Translation:AnimalsCells
    42. Li XZ, Roy CK, Moore MJ, Zamore PD. Defining piRNA primary transcripts. Cell Cycle. 2013 Jun 01; 12(11):1657-8. PMID: 23673320.
      Citations: 10     Fields:    Translation:AnimalsCells
    43. Jokhi V, Ashley J, Nunnari J, Noma A, Ito N, Wakabayashi-Ito N, Moore MJ, Budnik V. Torsin mediates primary envelopment of large ribonucleoprotein granules at the nuclear envelope. Cell Rep. 2013 Apr 25; 3(4):988-95. PMID: 23583177.
      Citations: 76     Fields:    Translation:HumansAnimalsCells
    44. Crawford DJ, Hoskins AA, Friedman LJ, Gelles J, Moore MJ. Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site. Proc Natl Acad Sci U S A. 2013 Apr 23; 110(17):6783-8. PMID: 23569281.
      Citations: 33     Fields:    Translation:AnimalsCells
    45. Li XZ, Roy CK, Dong X, Bolcun-Filas E, Wang J, Han BW, Xu J, Moore MJ, Schimenti JC, Weng Z, Zamore PD. An ancient transcription factor initiates the burst of piRNA production during early meiosis in mouse testes. Mol Cell. 2013 Apr 11; 50(1):67-81. PMID: 23523368.
      Citations: 159     Fields:    Translation:AnimalsCells
    46. Singh G, Kucukural A, Cenik C, Leszyk JD, Shaffer SA, Weng Z, Moore MJ. The Cellular EJC Interactome Reveals Higher-Order mRNP Structure and an EJC-SR Protein Nexus. Cell. 2012 11 09; 151(4):915-916. PMID: 30360293.
      Citations: 3     Fields:    
    47. Bicknell AA, Cenik C, Chua HN, Roth FP, Moore MJ. Introns in UTRs: why we should stop ignoring them. Bioessays. 2012 Dec; 34(12):1025-34. PMID: 23108796.
      Citations: 56     Fields:    Translation:HumansAnimalsCells
    48. Singh G, Kucukural A, Cenik C, Leszyk JD, Shaffer SA, Weng Z, Moore MJ. The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus. Cell. 2012 Nov 09; 151(4):750-764. PMID: 23084401.
      Citations: 172     Fields:    Translation:HumansCells
    49. Wu CH, Fallini C, Ticozzi N, Keagle PJ, Sapp PC, Piotrowska K, Lowe P, Koppers M, McKenna-Yasek D, Baron DM, Kost JE, Gonzalez-Perez P, Fox AD, Adams J, Taroni F, Tiloca C, Leclerc AL, Chafe SC, Mangroo D, Moore MJ, Zitzewitz JA, Xu ZS, van den Berg LH, Glass JD, Siciliano G, Cirulli ET, Goldstein DB, Salachas F, Meininger V, Rossoll W, Ratti A, Gellera C, Bosco DA, Bassell GJ, Silani V, Drory VE, Brown RH, Landers JE. Mutations in the profilin 1 gene cause familial amyotrophic lateral sclerosis. Nature. 2012 Aug 23; 488(7412):499-503. PMID: 22801503.
      Citations: 253     Fields:    Translation:HumansAnimalsCells
    50. Speese SD, Ashley J, Jokhi V, Nunnari J, Barria R, Li Y, Ataman B, Koon A, Chang YT, Li Q, Moore MJ, Budnik V. Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling. Cell. 2012 May 11; 149(4):832-46. PMID: 22579286.
      Citations: 182     Fields:    Translation:HumansAnimalsCells
    51. Hoskins AA, Moore MJ. The spliceosome: a flexible, reversible macromolecular machine. Trends Biochem Sci. 2012 May; 37(5):179-88. PMID: 22480731.
      Citations: 104     Fields:    Translation:HumansAnimalsCells
    52. Morello LG, Coltri PP, Quaresma AJ, Simabuco FM, Silva TC, Singh G, Nickerson JA, Oliveira CC, Moore MJ, Zanchin NI. The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing. PLoS One. 2011; 6(12):e29174. PMID: 22195017.
      Citations: 13     Fields:    Translation:HumansCells
    53. Hoskins AA, Gelles J, Moore MJ. New insights into the spliceosome by single molecule fluorescence microscopy. Curr Opin Chem Biol. 2011 Dec; 15(6):864-70. PMID: 22057211.
      Citations: 14     Fields:    Translation:AnimalsCells
    54. Cenik C, Chua HN, Zhang H, Tarnawsky SP, Akef A, Derti A, Tasan M, Moore MJ, Palazzo AF, Roth FP. Genome analysis reveals interplay between 5'UTR introns and nuclear mRNA export for secretory and mitochondrial genes. PLoS Genet. 2011 Apr; 7(4):e1001366. PMID: 21533221.
      Citations: 33     Fields:    Translation:HumansCells
    55. Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ. Ordered and dynamic assembly of single spliceosomes. Science. 2011 Mar 11; 331(6022):1289-95. PMID: 21393538.
      Citations: 130     Fields:    Translation:AnimalsCells
    56. Sephton CF, Cenik C, Kucukural A, Dammer EB, Cenik B, Han Y, Dewey CM, Roth FP, Herz J, Peng J, Moore MJ, Yu G. Identification of neuronal RNA targets of TDP-43-containing ribonucleoprotein complexes. J Biol Chem. 2011 Jan 14; 286(2):1204-15. PMID: 21051541.
      Citations: 215     Fields:    Translation:HumansAnimalsCells
    57. Lemmens R, Moore MJ, Al-Chalabi A, Brown RH, Robberecht W. RNA metabolism and the pathogenesis of motor neuron diseases. Trends Neurosci. 2010 May; 33(5):249-58. PMID: 20227117.
      Citations: 38     Fields:    Translation:HumansAnimalsCells
    58. Albert BJ, McPherson PA, O'Brien K, Czaicki NL, Destefino V, Osman S, Li M, Day BW, Grabowski PJ, Moore MJ, Vogt A, Koide K. Meayamycin inhibits pre-messenger RNA splicing and exhibits picomolar activity against multidrug-resistant cells. Mol Cancer Ther. 2009 Aug; 8(8):2308-18. PMID: 19671752.
      Citations: 52     Fields:    Translation:HumansCells
    59. Cole SE, LaRiviere FJ, Merrikh CN, Moore MJ. A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decay. Mol Cell. 2009 May 14; 34(4):440-50. PMID: 19481524.
      Citations: 87     Fields:    Translation:HumansAnimalsCells
    60. Moore MJ, Proudfoot NJ. Pre-mRNA processing reaches back to transcription and ahead to translation. Cell. 2009 Feb 20; 136(4):688-700. PMID: 19239889.
      Citations: 451     Fields:    Translation:HumansAnimalsCells
    61. Bramham CR, Worley PF, Moore MJ, Guzowski JF. The immediate early gene arc/arg3.1: regulation, mechanisms, and function. J Neurosci. 2008 Nov 12; 28(46):11760-7. PMID: 19005037.
      Citations: 226     Fields:    Translation:HumansAnimalsCells
    62. O'Brien K, Matlin AJ, Lowell AM, Moore MJ. The biflavonoid isoginkgetin is a general inhibitor of Pre-mRNA splicing. J Biol Chem. 2008 Nov 28; 283(48):33147-54. PMID: 18826947.
      Citations: 89     Fields:    Translation:HumansCells
    63. Rozovsky N, Butterworth AC, Moore MJ. Interactions between eIF4AI and its accessory factors eIF4B and eIF4H. RNA. 2008 Oct; 14(10):2136-48. PMID: 18719248.
      Citations: 47     Fields:    Translation:HumansCells
    64. Crawford DJ, Hoskins AA, Friedman LJ, Gelles J, Moore MJ. Visualizing the splicing of single pre-mRNA molecules in whole cell extract. RNA. 2008 Jan; 14(1):170-9. PMID: 18025254.
      Citations: 44     Fields:    Translation:HumansAnimalsCells
    65. Giorgi C, Yeo GW, Stone ME, Katz DB, Burge C, Turrigiano G, Moore MJ. The EJC factor eIF4AIII modulates synaptic strength and neuronal protein expression. Cell. 2007 Jul 13; 130(1):179-91. PMID: 17632064.
      Citations: 160     Fields:    Translation:HumansAnimalsCells
    66. Giorgi C, Moore MJ. The nuclear nurture and cytoplasmic nature of localized mRNPs. Semin Cell Dev Biol. 2007 Apr; 18(2):186-93. PMID: 17459736.
      Citations: 59     Fields:    Translation:HumansAnimalsCells
    67. Matlin AJ, Moore MJ. Spliceosome assembly and composition. Adv Exp Med Biol. 2007; 623:14-35. PMID: 18380338.
      Citations: 44     Fields:    Translation:HumansAnimalsCells
    68. LaRiviere FJ, Cole SE, Ferullo DJ, Moore MJ. A late-acting quality control process for mature eukaryotic rRNAs. Mol Cell. 2006 Nov 17; 24(4):619-26. PMID: 17188037.
      Citations: 70     Fields:    Translation:AnimalsCells
    69. Stroupe ME, Tange TØ, Thomas DR, Moore MJ, Grigorieff N. The three-dimensional arcitecture of the EJC core. J Mol Biol. 2006 Jul 21; 360(4):743-9. PMID: 16797590.
      Citations: 16     Fields:    Translation:HumansCells
    70. Shibuya T, Tange TØ, Stroupe ME, Moore MJ. Mutational analysis of human eIF4AIII identifies regions necessary for exon junction complex formation and nonsense-mediated mRNA decay. RNA. 2006 Mar; 12(3):360-74. PMID: 16495234.
      Citations: 33     Fields:    Translation:HumansCells
    71. Tange TØ, Shibuya T, Jurica MS, Moore MJ. Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core. RNA. 2005 Dec; 11(12):1869-83. PMID: 16314458.
      Citations: 100     Fields:    Translation:HumansCells
    72. Schroder PA, Moore MJ. Association of ribosomal proteins with nascent transcripts in S. cerevisiae. RNA. 2005 Oct; 11(10):1521-9. PMID: 16199762.
      Citations: 12     Fields:    Translation:AnimalsCells
    73. Moore MJ. From birth to death: the complex lives of eukaryotic mRNAs. Science. 2005 Sep 02; 309(5740):1514-8. PMID: 16141059.
      Citations: 480     Fields:    Translation:AnimalsCells
    74. Du H, Tardiff DF, Moore MJ, Rosbash M. Effects of the U1C L13 mutation and temperature regulation of yeast commitment complex formation. Proc Natl Acad Sci U S A. 2004 Oct 12; 101(41):14841-6. PMID: 15465910.
      Citations: 9     Fields:    Translation:Animals
    75. Tange TØ, Nott A, Moore MJ. The ever-increasing complexities of the exon junction complex. Curr Opin Cell Biol. 2004 Jun; 16(3):279-84. PMID: 15145352.
      Citations: 129     Fields:    Translation:HumansAnimalsCells
    76. Shibuya T, Tange TØ, Sonenberg N, Moore MJ. eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay. Nat Struct Mol Biol. 2004 Apr; 11(4):346-51. PMID: 15034551.
      Citations: 119     Fields:    Translation:HumansCells
    77. Jurica MS, Sousa D, Moore MJ, Grigorieff N. Three-dimensional structure of C complex spliceosomes by electron microscopy. Nat Struct Mol Biol. 2004 Mar; 11(3):265-9. PMID: 14981503.
      Citations: 33     Fields:    Translation:HumansCells
    78. Nott A, Le Hir H, Moore MJ. Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. Genes Dev. 2004 Jan 15; 18(2):210-22. PMID: 14752011.
      Citations: 189     Fields:    Translation:HumansAnimalsCells
    79. Jurica MS, Moore MJ. Pre-mRNA splicing: awash in a sea of proteins. Mol Cell. 2003 Jul; 12(1):5-14. PMID: 12887888.
      Citations: 477     Fields:    Translation:HumansAnimalsCells
    80. Nott A, Meislin SH, Moore MJ. A quantitative analysis of intron effects on mammalian gene expression. RNA. 2003 May; 9(5):607-17. PMID: 12702819.
      Citations: 174     Fields:    Translation:HumansAnimalsCells
    81. Le Hir H, Nott A, Moore MJ. How introns influence and enhance eukaryotic gene expression. Trends Biochem Sci. 2003 Apr; 28(4):215-20. PMID: 12713906.
      Citations: 266     Fields:    Translation:AnimalsCells
    82. Jurica MS, Moore MJ. Capturing splicing complexes to study structure and mechanism. Methods. 2002 Nov; 28(3):336-45. PMID: 12431437.
      Citations: 14     Fields:    Translation:Cells
    83. Reichert VL, Le Hir H, Jurica MS, Moore MJ. 5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly. Genes Dev. 2002 Nov 01; 16(21):2778-91. PMID: 12414731.
      Citations: 68     Fields:    Translation:HumansCells
    84. Moore MJ. RNA events. No end to nonsense. Science. 2002 Oct 11; 298(5592):370-1. PMID: 12376689.
      Citations: 4     Fields:    Translation:Cells
    85. Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA. 2002 Apr; 8(4):426-39. PMID: 11991638.
      Citations: 180     Fields:    Translation:Cells
    86. Moore M. Nuclear RNA turnover. Cell. 2002 Feb 22; 108(4):431-4. PMID: 11909514.
      Citations: 25     Fields:    Translation:AnimalsCells
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