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John Leszyk PhD

TitleAssociate Professor
InstitutionUniversity of Massachusetts Medical School
DepartmentBiochemistry and Molecular Pharmacology
AddressFuller Building
222 Maple Avenue
Worcester MA 01655
Phone508-856-7533
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentBiochemistry and Molecular Pharmacology


    Collapse Biography 
    Collapse education and training
    State University of New York, Oswego, Oswego, NY, United StatesBSChemistry
    Clarkson University, Potsdam, NY, United StatesPHDBiochemistry

    Collapse Overview 
    Collapse overview

    Academic Background

    Ph.D., Clarkson University, 1988
    B.S., SUNY at Oswego, 1984

    Proteomics and Mass Spectrometry

    Photo: John D. Leszyk I have been involved with analytical protein chemistry since my graduate work in the mid 1980’s where I used a first generation gas-phase Edman sequencer to determine amino acid sequences and chemical cross-linking sites of the subunits of the Troponin complex which are responsible for the Ca2+ regulation of muscle contraction. I then did a short post-doctoral study at the University Of Maryland School Of Medicine where I continued to pursue studies of the muscle regulatory subunits of the Troponin complex. In 1989 I came to the Worcester Foundation for Experimental Biology to set up their protein chemistry laboratory where I offered Edman sequencing, amino acid analysis and peptide synthesis services. Over time my approach to characterizing proteins evolved from a largely microchemical to a mass spectrometry based approach. The need for Edman sequencing diminished significantly due to the development of mass spectrometry based protein identification techniques which utilized both MS and tandem (MS/MS) data along with molecular weight search engine (MOWSE) software. In 1995 I acquired my first Matrix Assisted Laser Desorption/Ionization (MALDI) mass spectrometer. Since then I had developed a strong interest in the applications of MALDI time-of-flight (TOF) mass spectrometry in the area of protein characterization. In 1997 the Worcester Foundation merged with the University of Massachusetts Medical School where I became a research assistant professor in the department of Biochemistry and Molecular Pharmacology. My laboratory had continued as an independent self-supporting proteomics mass spectrometry core facility until 2010 when it was combined with two other proteomics facilities on campus to form an integrated Proteomics and Mass Spectrometry Facility. As Associate Director of the new facility I have been largely involved with the setup and implementation of various software pipelines for both the quantitative and qualitative aspects of proteomics analyses. I have also been involved with setup and optimization of the nanoflow LC/MS/MS experiment on the facility’s Thermo Velos and Q Exactive Orbitrap mass spectrometers. With the new instrumentation and software pipelines more complex proteomics experiments are now being carried out routinely by the facility. Such experiments inlcude characterization of post-translational modifications of complex samples, both label free and labeled quantitation experiments (iTRAQ, and SILAC), and the identification of chemical cross-links.


    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Rittershaus ESC, Baek SH, Krieger IV, Nelson SJ, Cheng YS, Nambi S, Baker RE, Leszyk JD, Shaffer SA, Sacchettini JC, Sassetti CM. A Lysine Acetyltransferase Contributes to the Metabolic Adaptation to Hypoxia in Mycobacterium tuberculosis. Cell Chem Biol. 2018 Oct 04. PMID: 30318462.
      View in: PubMed
    2. Maziuk BF, Apicco DJ, Cruz AL, Jiang L, Ash PEA, da Rocha EL, Zhang C, Yu WH, Leszyk J, Abisambra JF, Li H, Wolozin B. RNA binding proteins co-localize with small tau inclusions in tauopathy. Acta Neuropathol Commun. 2018 Aug 01; 6(1):71. PMID: 30068389.
      View in: PubMed
    3. Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ. C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2. Nat Methods. 2018 May 07. PMID: 29735996.
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    4. Sakatos A, Babunovic GH, Chase MR, Dills A, Leszyk J, Rosebrock T, Bryson B, Fortune SM. Posttranslational modification of a histone-like protein regulates phenotypic resistance to isoniazid in mycobacteria. Sci Adv. 2018 May; 4(5):eaao1478. PMID: 29732401.
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    5. Cormier N, McGlone JJ, Leszyk J, Hardy DM. Immunocontraceptive target repertoire defined by systematic identification of sperm membrane alloantigens in a single species. PLoS One. 2018; 13(1):e0190891. PMID: 29342175.
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    6. Apicco DJ, Ash PEA, Maziuk B, LeBlang C, Medalla M, Al Abdullatif A, Ferragud A, Botelho E, Ballance HI, Dhawan U, Boudeau S, Cruz AL, Kashy D, Wong A, Goldberg LR, Yazdani N, Zhang C, Ung CY, Tripodis Y, Kanaan NM, Ikezu T, Cottone P, Leszyk J, Li H, Luebke J, Bryant CD, Wolozin B. Reducing the RNA binding protein TIA1 protects against tau-mediated neurodegeneration in vivo. Nat Neurosci. 2018 Jan; 21(1):72-80. PMID: 29273772.
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    7. Prokaeva T, Akar H, Spencer B, Havasi A, Cui H, O'Hara CJ, Gursky O, Leszyk J, Steffen M, Browning S, Rosenberg A, Connors LH. Hereditary Renal Amyloidosis Associated With a Novel Apolipoprotein A-II Variant. Kidney Int Rep. 2017 Nov; 2(6):1223-1232. PMID: 29270531.
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    8. Chen L, Paquette N, Mamoor S, Rus F, Nandy A, Leszyk J, Shaffer SA, Silverman N. Innate immune signaling in Drosophila is regulated by TGFß-activated kinase (Tak1)-triggered ubiquitin editing. J Biol Chem. 2017 Apr 04. PMID: 28377500.
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    9. Shin A, Shin B, Shin JW, Kim KH, Atwal RS, Hope JM, Gillis T, Leszyk JD, Shaffer SA, Lee R, Kwak S, MacDonald ME, Gusella JF, Seong IS, Lee JM. Novel allele-specific quantification methods reveal no effects of adult onset CAG repeats on HTT mRNA and protein levels. Hum Mol Genet. 2017 Feb 06. PMID: 28165127.
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    10. Friesen WJ, Trotta CR, Tomizawa Y, Zhuo J, Johnson B, Sierra J, Roy B, Weetall M, Hedrick J, Sheedy J, Takasugi J, Moon YC, Babu S, Baiazitov R, Leszyk JD, Davis TW, Peltz SW, Welch EM. The nucleoside analog clitocine is a potent and efficacious readthrough agent. RNA. 2017 Jan 17. PMID: 28096517.
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    11. Cheng B, Zhou Q, Weng L, Leszyk JD, Greenberg MM, Tse-Dinh YC. Identification of proximal sites for unwound DNA substrate in Escherichia coli topoisomerase I with oxidative crosslinking. FEBS Lett. 2017 Jan; 591(1):28-38. PMID: 27926785.
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    12. Haraszti RA, Didiot MC, Sapp E, Leszyk J, Shaffer SA, Rockwell HE, Gao F, Narain NR, DiFiglia M, Kiebish MA, Aronin N, Khvorova A. High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources. J Extracell Vesicles. 2016; 5:32570. PMID: 27863537.
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    13. Roy B, Friesen WJ, Tomizawa Y, Leszyk JD, Zhuo J, Johnson B, Dakka J, Trotta CR, Xue X, Mutyam V, Keeling KM, Mobley JA, Rowe SM, Bedwell DM, Welch EM, Jacobson A. Ataluren stimulates ribosomal selection of near-cognate tRNAs to promote nonsense suppression. Proc Natl Acad Sci U S A. 2016 Nov 01; 113(44):12508-12513. PMID: 27702906.
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    14. Vanderweyde T, Apicco DJ, Youmans-Kidder K, Ash PE, Cook C, Lummertz da Rocha E, Jansen-West K, Frame AA, Citro A, Leszyk JD, Ivanov P, Abisambra JF, Steffen M, Li H, Petrucelli L, Wolozin B. Interaction of tau with the RNA-Binding Protein TIA1 Regulates tau Pathophysiology and Toxicity. Cell Rep. 2016 May 17; 15(7):1455-66. PMID: 27160897.
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    15. Hayashi JM, Luo CY, Mayfield JA, Hsu T, Fukuda T, Walfield AL, Giffen SR, Leszyk JD, Baer CE, Bennion OT, Madduri A, Shaffer SA, Aldridge BB, Sassetti CM, Sandler SJ, Kinoshita T, Moody DB, Morita YS. Spatially distinct and metabolically active membrane domain in mycobacteria. Proc Natl Acad Sci U S A. 2016 May 10; 113(19):5400-5. PMID: 27114527.
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    16. Specht CA, Lee CK, Huang H, Tipper DJ, Shen ZT, Lodge JK, Leszyk J, Ostroff GR, Levitz SM. Protection against Experimental Cryptococcosis following Vaccination with Glucan Particles Containing Cryptococcus Alkaline Extracts. MBio. 2015 Dec 22; 6(6):e01905-15. PMID: 26695631.
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    17. Colon K, Perez-Laspiur J, Quiles R, Rodriguez Y, Wojna V, Shaffer SA, Leszyk J, Skolasky RL, Melendez LM. Macrophage secretome from women with HIV-associated neurocognitive disorders. Proteomics Clin Appl. 2016 Feb; 10(2):136-43. PMID: 26220577.
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    18. Cantres-Rosario YM, Hernandez N, Negron K, Perez-Laspiur J, Leszyk J, Shaffer SA, Meléndez LM. Interacting partners of macrophage-secreted cathepsin B contribute to HIV-induced neuronal apoptosis. AIDS. 2015 Oct 23; 29(16):2081-92. PMID: 26208400.
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    19. Bennett KL, Wang X, Bystrom CE, Chambers MC, Andacht TM, Dangott LJ, Elortza F, Leszyk J, Molina H, Moritz RL, Phinney BS, Thompson JW, Bunger MK, Tabb DL. The 2012/2013 ABRF Proteomic Research Group Study: Assessing Longitudinal Intralaboratory Variability in Routine Peptide Liquid Chromatography Tandem Mass Spectrometry Analyses. Mol Cell Proteomics. 2015 Dec; 14(12):3299-309. PMID: 26435129.
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    20. Nasipak BT, Padilla-Benavides T, Green KM, Leszyk JD, Mao W, Konda S, Sif S, Shaffer SA, Ohkawa Y, Imbalzano AN. Opposing calcium-dependent signalling pathways control skeletal muscle differentiation by regulating a chromatin remodelling enzyme. Nat Commun. 2015 Jun 17; 6:7441. PMID: 26081415.
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    21. Roy B, Leszyk JD, Mangus DA, Jacobson A. Nonsense suppression by near-cognate tRNAs employs alternative base pairing at codon positions 1 and 3. Proc Natl Acad Sci U S A. 2015 Mar 10; 112(10):3038-43. PMID: 25733896.
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    22. Smith TC, Fridy PC, Li Y, Basil S, Arjun S, Friesen RM, Leszyk J, Chait BT, Rout MP, Luna EJ. Supervillin binding to myosin II and synergism with anillin are required for cytokinesis. Mol Biol Cell. 2013 Dec; 24(23):3603-19. PMID: 24088567.
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    23. Jones KA, Kim PD, Patel BB, Kelsen SG, Braverman A, Swinton DJ, Gafken PR, Jones LA, Lane WS, Neveu JM, Leung HC, Shaffer SA, Leszyk JD, Stanley BA, Fox TE, Stanley A, Hall MJ, Hampel H, South CD, de la Chapelle A, Burt RW, Jones DA, Kopelovich L, Yeung AT. Immunodepletion plasma proteomics by tripleTOF 5600 and Orbitrap elite/LTQ-Orbitrap Velos/Q exactive mass spectrometers. J Proteome Res. 2013 Oct 04; 12(10):4351-65. PMID: 24004147.
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    24. Kiso WK, Selvaraj V, Nagashima J, Asano A, Brown JL, Schmitt DL, Leszyk J, Travis AJ, Pukazhenthi BS. Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality. PLoS One. 2013; 8(8):e71033. PMID: 23976974.
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    25. Singh G, Kucukural A, Cenik C, Leszyk JD, Shaffer SA, Weng Z, Moore MJ. The Cellular EJC Interactome Reveals Higher-Order mRNP Structure and an EJC-SR Protein Nexus. Cell. 2012 Nov 09; 151(4):915-916. PMID: 30360293.
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    26. Singh G, Kucukural A, Cenik C, Leszyk JD, Shaffer SA, Weng Z, Moore MJ. The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus. Cell. 2012 Nov 9; 151(4):750-64. PMID: 23084401.
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    27. Li Y, Powell DA, Shaffer SA, Rasko DA, Pelletier MR, Leszyk JD, Scott AJ, Masoudi A, Goodlett DR, Wang X, Raetz CR, Ernst RK. LPS remodeling is an evolved survival strategy for bacteria. Proc Natl Acad Sci U S A. 2012 May 29; 109(22):8716-21. PMID: 22586119.
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    28. Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y, Leszyk J, Zhang B, Budnik V. Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. J Biol Chem. 2012 May 11; 287(20):16820-34. PMID: 22437826.
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    29. Kruger AJ, Yang C, Tam SW, Hinerfeld D, Evans JE, Green KM, Leszyk J, Yang K, Guberski DL, Mordes JP, Greiner DL, Rossini AA, Bortell R. Haptoglobin as an early serum biomarker of virus-induced autoimmune type 1 diabetes in biobreeding diabetes resistant and LEW1.WR1 rats. Exp Biol Med (Maywood). 2010 Nov; 235(11):1328-37. PMID: 20975081.
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    30. Leszyk JD. Evaluation of the new MALDI matrix 4-chloro-alpha-cyanocinnamic acid. J Biomol Tech. 2010 Jul; 21(2):81-91. PMID: 20592871.
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    31. Romero R, Kusanovic JP, Gotsch F, Erez O, Vaisbuch E, Mazaki-Tovi S, Moser A, Tam S, Leszyk J, Master SR, Juhasz P, Pacora P, Ogge G, Gomez R, Yoon BH, Yeo L, Hassan SS, Rogers WT. Isobaric labeling and tandem mass spectrometry: a novel approach for profiling and quantifying proteins differentially expressed in amniotic fluid in preterm labor with and without intra-amniotic infection/inflammation. J Matern Fetal Neonatal Med. 2010 Apr; 23(4):261-80. PMID: 19670042.
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    32. Zhou QL, Jiang ZY, Holik J, Chawla A, Hagan GN, Leszyk J, Czech MP. Akt substrate TBC1D1 regulates GLUT1 expression through the mTOR pathway in 3T3-L1 adipocytes. Biochem J. 2008 May 1; 411(3):647-55. PMID: 18215134.
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    33. Davis MA, Hinerfeld D, Joseph S, Hui YH, Huang NH, Leszyk J, Rutherford-Bethard J, Tam SW. Proteomic analysis of rat liver phosphoproteins after treatment with protein kinase inhibitor H89 (N-(2-[p-bromocinnamylamino-]ethyl)-5-isoquinolinesulfonamide). J Pharmacol Exp Ther. 2006 Aug; 318(2):589-95. PMID: 16687476.
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    34. Pantua H, McGinnes LW, Leszyk J, Morrison TG. Characterization of an alternate form of Newcastle disease virus fusion protein. J Virol. 2005 Sep; 79(18):11660-70. PMID: 16140743.
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    35. Pazour GJ, Agrin N, Leszyk J, Witman GB. Proteomic analysis of a eukaryotic cilium. J Cell Biol. 2005 Jul 4; 170(1):103-13. PMID: 15998802.
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    36. Jiang ZY, Zhou QL, Holik J, Patel S, Leszyk J, Coleman K, Chouinard M, Czech MP. Identification of WNK1 as a substrate of Akt/protein kinase B and a negative regulator of insulin-stimulated mitogenesis in 3T3-L1 cells. J Biol Chem. 2005 Jun 3; 280(22):21622-8. PMID: 15799971.
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    37. Wilson-Fritch L, Nicoloro S, Chouinard M, Lazar MA, Chui PC, Leszyk J, Straubhaar J, Czech MP, Corvera S. Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone. J Clin Invest. 2004 Nov; 114(9):1281-9. PMID: 15520860.
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    38. Blüher M, Wilson-Fritch L, Leszyk J, Laustsen PG, Corvera S, Kahn CR. Role of insulin action and cell size on protein expression patterns in adipocytes. J Biol Chem. 2004 Jul 23; 279(30):31902-9. PMID: 15131120.
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    39. Guilherme A, Soriano NA, Bose S, Holik J, Bose A, Pomerleau DP, Furcinitti P, Leszyk J, Corvera S, Czech MP. EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton. J Biol Chem. 2004 Mar 12; 279(11):10593-605. PMID: 14676205.
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    40. Semiz S, Park JG, Nicoloro SM, Furcinitti P, Zhang C, Chawla A, Leszyk J, Czech MP. Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules. EMBO J. 2003 May 15; 22(10):2387-99. PMID: 12743033.
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    41. Wilson-Fritch L, Burkart A, Bell G, Mendelson K, Leszyk J, Nicoloro S, Czech M, Corvera S. Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone. Mol Cell Biol. 2003 Feb; 23(3):1085-94. PMID: 12529412.
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    42. Nebl T, Pestonjamasp KN, Leszyk JD, Crowley JL, Oh SW, Luna EJ. Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes. J Biol Chem. 2002 Nov 8; 277(45):43399-409. PMID: 12202484.
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    43. Luo Y, Leszyk J, Li B, Li Z, Gergely J, Tao T. Troponin-I interacts with the Met47 region of skeletal muscle actin. Implications for the mechanism of thin filament regulation by calcium. J Mol Biol. 2002 Feb 22; 316(3):429-34. PMID: 11866508.
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    44. Park JG, Bose A, Leszyk J, Czech MP. PYK2 as a mediator of endothelin-1/G alpha 11 signaling to GLUT4 glucose transporters. J Biol Chem. 2001 Dec 21; 276(51):47751-4. PMID: 11602570.
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    45. Vaughan PS, Leszyk JD, Vaughan KT. Cytoplasmic dynein intermediate chain phosphorylation regulates binding to dynactin. J Biol Chem. 2001 Jul 13; 276(28):26171-9. PMID: 11340075.
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    46. Holaska JM, Black BE, Love DC, Hanover JA, Leszyk J, Paschal BM. Calreticulin Is a receptor for nuclear export. J Cell Biol. 2001 Jan 8; 152(1):127-40. PMID: 11149926.
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    47. Luo Y, Leszyk J, Li B, Gergely J, Tao T. Proximity relationships between residue 6 of troponin I and residues in troponin C: further evidence for extended conformation of troponin C in the troponin complex. Biochemistry. 2000 Dec 19; 39(50):15306-15. PMID: 11112516.
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    48. Guilherme A, Emoto M, Buxton JM, Bose S, Sabini R, Theurkauf WE, Leszyk J, Czech MP. Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes. J Biol Chem. 2000 Dec 8; 275(49):38151-9. PMID: 10950952.
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    49. Baran DT, Quail JM, Ray R, Leszyk J, Honeyman T. Annexin II is the membrane receptor that mediates the rapid actions of 1alpha,25-dihydroxyvitamin D(3). J Cell Biochem. 2000 Apr; 78(1):34-46. PMID: 10797564.
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    50. Luo Y, Leszyk J, Qian Y, Gergely J, Tao T. Residues 48 and 82 at the N-terminal hydrophobic pocket of rabbit skeletal muscle troponin-C photo-cross-link to Met121 of troponin-I. Biochemistry. 1999 May 18; 38(20):6678-88. PMID: 10350487.
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    51. Szymanska G, Leszyk JD, O'Connor CM. Carboxyl methylation of deamidated calmodulin increases its stability in Xenopus oocyte cytoplasm. Implications for protein repair. J Biol Chem. 1998 Oct 23; 273(43):28516-23. PMID: 9774482.
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    52. Choi JY, van Wijnen AJ, Aslam F, Leszyk JD, Stein JL, Stein GS, Lian JB, Penman S. Developmental association of the beta-galactoside-binding protein galectin-1 with the nuclear matrix of rat calvarial osteoblasts. J Cell Sci. 1998 Oct; 111 ( Pt 20):3035-43. PMID: 9739077.
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    53. San Agustin JT, Leszyk JD, Nuwaysir LM, Witman GB. The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence. J Biol Chem. 1998 Sep 18; 273(38):24874-83. PMID: 9733793.
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    54. Leszyk J, Tao T, Nuwaysir LM, Gergely J. Identification of the photocrosslinking sites in troponin-I with 4-maleimidobenzophenone labelled mutant troponin-Cs having single cysteines at positions 158 and 21. J Muscle Res Cell Motil. 1998 Jun; 19(5):479-90. PMID: 9682135.
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    55. Ross DD, Gao Y, Yang W, Leszyk J, Shively J, Doyle LA. The 95-kilodalton membrane glycoprotein overexpressed in novel multidrug-resistant breast cancer cells is NCA, the nonspecific cross-reacting antigen of carcinoembryonic antigen. Cancer Res. 1997 Dec 15; 57(24):5460-4. PMID: 9407950.
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    56. Kashina AS, Scholey JM, Leszyk JD, Saxton WM. An essential bipolar mitotic motor. Nature. 1996 Nov 21; 384(6606):225. PMID: 8918872.
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    57. Deyst KA, Bowe MA, Leszyk JD, Fallon JR. The alpha-dystroglycan-beta-dystroglycan complex. Membrane organization and relationship to an agrin receptor. J Biol Chem. 1995 Oct 27; 270(43):25956-9. PMID: 7592785.
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    58. Vater CA, Bartle LM, Leszyk JD, Lambert JM, Goldmacher VS. Ricin A chain can be chemically cross-linked to the mammalian ribosomal proteins L9 and L10e. J Biol Chem. 1995 May 26; 270(21):12933-40. PMID: 7759553.
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    59. Biswas EE, Chen PH, Leszyk J, Biswas SB. Biochemical and genetic characterization of a replication protein A dependent DNA helicase from the yeast, Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1995 Jan 26; 206(3):850-6. PMID: 7832796.
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    60. Sewell TJ, Lam E, Martin MM, Leszyk J, Weidner J, Calaycay J, Griffin P, Williams H, Hung S, Cryan J, et al. Inhibition of calcineurin by a novel FK-506-binding protein. J Biol Chem. 1994 Aug 19; 269(33):21094-102. PMID: 7520438.
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    61. Cao QP, Pitt S, Leszyk J, Baril EF. DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen. Biochemistry. 1994 Jul 19; 33(28):8548-57. PMID: 8031790.
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    62. Bowe MA, Deyst KA, Leszyk JD, Fallon JR. Identification and purification of an agrin receptor from Torpedo postsynaptic membranes: a heteromeric complex related to the dystroglycans. Neuron. 1994 May; 12(5):1173-80. PMID: 8185951.
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    63. Vissavajjhala P, Leszyk JD, Lin-Goerke J, Ross AH. Structural domains of the extracellular domain of human nerve growth factor receptor detected by partial proteolysis. Arch Biochem Biophys. 1992 Apr; 294(1):244-52. PMID: 1372492.
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    64. Barouch WW, Breese KE, Davidoff SA, Leszyk J, Szent-Györgyi AG, Theibert JL, Collins JH. Amino acid sequences of myosin essential and regulatory light chains from two clam species: comparison with other molluscan myosin light chains. J Muscle Res Cell Motil. 1991 Aug; 12(4):321-32. PMID: 1939600.
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    65. Collins JH, Leszyk J, Mornet D, Audemard E. Turkey gizzard caldesmon: complete sequence of a C-terminal thrombic fragment that binds actin, tropomyosin and calmodulin. Protein Seq Data Anal. 1991 Jul; 4(1):29-32. PMID: 1924267.
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    66. Leszyk J, Grabarek Z, Gergely J, Collins JH. Characterization of zero-length cross-links between rabbit skeletal muscle troponin C and troponin I: evidence for direct interaction between the inhibitory region of troponin I and the NH2-terminal, regulatory domain of troponin C. Biochemistry. 1990 Jan 9; 29(1):299-304. PMID: 2108719.
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    67. Leszyk J, Mornet D, Audemard E, Collins JH. Caldesmon structure and function: sequence analysis of a 35 kilodalton actin- and calmodulin-binding fragment from the C-terminus of the turkey gizzard protein. Biochem Biophys Res Commun. 1989 May 15; 160(3):1371-8. PMID: 2730648.
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    68. Leszyk J, Mornet D, Audemard E, Collins JH. Amino acid sequence of a 15 kilodalton actin-binding fragment of turkey gizzard caldesmon: similarity with dystrophin, tropomyosin and the tropomyosin-binding region of troponin T. Biochem Biophys Res Commun. 1989 Apr 14; 160(1):210-6. PMID: 2653315.
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    69. Leszyk J, Collins JH, Leavis PC, Tao T. Cross-linking of rabbit skeletal muscle troponin subunits: labeling of cysteine-98 of troponin C with 4-maleimidobenzophenone and analysis of products formed in the binary complex with troponin T and the ternary complex with troponins I and T. Biochemistry. 1988 Sep 6; 27(18):6983-7. PMID: 3196696.
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    70. Leszyk J, Dumaswala R, Potter JD, Collins JH. Amino acid sequence of bovine cardiac troponin I. Biochemistry. 1988 Apr 19; 27(8):2821-7. PMID: 3042023.
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    71. Collins JH, Leszyk J. The "gamma subunit" of Na,K-ATPase: a small, amphiphilic protein with a unique amino acid sequence. Biochemistry. 1987 Dec 29; 26(26):8665-8. PMID: 2831947.
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    72. Leszyk J, Dumaswala R, Potter JD, Gusev NB, Verin AD, Tobacman LS, Collins JH. Bovine cardiac troponin T: amino acid sequences of the two isoforms. Biochemistry. 1987 Nov 3; 26(22):7035-42. PMID: 3122824.
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    73. Leszyk J, Collins JH, Leavis PC, Tao T. Cross-linking of rabbit skeletal muscle troponin with the photoactive reagent 4-maleimidobenzophenone: identification of residues in troponin I that are close to cysteine-98 of troponin C. Biochemistry. 1987 Nov 3; 26(22):7042-7. PMID: 3427058.
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    74. Collins JH, Jakes R, Kendrick-Jones J, Leszyk J, Barouch W, Theibert JL, Spiegel J, Szent-Györgyi AG. Amino acid sequence of myosin essential light chain from the scallop Aquipecten irradians. Biochemistry. 1986 Nov 18; 25(23):7651-6. PMID: 3801438.
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