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Dannel McCollum PhD

InstitutionUMass Chan Medical School
DepartmentBiochemistry and Molecular Biotechnology
AddressUMass Chan Medical School
364 Plantation Street
Worcester MA 01605
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentImmunology and Microbiology Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentPostbaccalaureate Research Education Program

    Collapse Biography 
    Collapse education and training
    University of Illinois, Chicago, Chicago, IL, United StatesBA
    University of Illinois, Urbana-Champaign, Champaign, IL, United StatesBSBiochemistry
    University of California, San Diego, San Diego, CA, United StatesPHDBiology

    Collapse Overview 
    Collapse overview

    Role of mechanosensors and Hippo pathway signaling in controlling cell fate decisions

    The main interest of my lab is in understanding how the Hippo signaling pathway controls cellular responses to mechanical forces. The Hippo pathway is a protein kinase-mediated network that regulates cell proliferation, contact inhibition, apoptosis, stem cell maintenance and differentiation, and tissue regeneration. Misregulation of this pathway has deleterious effects on cells and organisms and can lead to cancer.  In the canonical pathway, MST1/2 kinases activate the LATS1/2 kinases, which in turn phosphorylate and inhibit the transcriptional co-activator YAP by causing its translocation from the nucleus to the cytoplasm. In the nucleus, YAP promotes cell survival and proliferation. YAP nuclear localization is regulated by diverse stimuli that affect the mechanical environment of the cell such as cell crowding, substrate stiffness, tension, and cell detachment. The ability to sense these types of changes to the mechanical environment is crucial for regulation of stem cell niches, wound healing, and contact inhibition of growth.

    The Hippo pathway is known to monitor the mechanical environment both indirectly through the effects of various stimuli on the actin cytoskeleton, and more directly by sensing tension across tissues. My lab’s focus has been on determining how F-actin levels and tension at cell-cell junctions are sensed, and how these signals are transduced to control Hippo signaling. We identified the angiomotin proteins as candidate Hippo pathway sensors for F-actin levels. Our studies have also uncovered new Hippo pathway regulators at cell-cell junctions that may directly sense tension across tissues. Current research in the lab is focusing on understanding the molecular mechanisms underlying F-actin and tension sensing, and how signals from separate sensors are integrated to generate cellular responses to mechanical stimuli.

    Collapse Rotation Projects

    Rotation Projects

    Analog sensitive LATS kinase:  The goal of this project is to use CRISPR based genome modification to make mutations in the endogenous LATS kinase that cause it to be inhibited by a synthetic ATP analog. This will allow LATS kinase activity to be turned on and off using the ATP analog. The analog sensitive cell line will be a valuable tool for studying Hippo pathway responses to various stimuli and will be used to test whether dephosphorylation of LATS substrates is an important regulatory mechanism. This project will also set the stage for future experiments to create mice that have analog sensitive versions of LATS. These mice will be used to test whether LATS inhibition is a viable treatment for regenerative therapies for the heart, gut, pancreas and other organs.

    TRIP6 and Angiomotin interacting proteins: We know that cells monitor their mechanical environment by sensing F-actin levels and by measuring tension at cell-cell junctions. We have identified angiomotins as sensors for F-actin and the TRIP6 protein as part of a tension sensor at cell-cell junctions. However, to understand how these sensors work, we need know the network of proteins that they each interact with, and how those networks change in response to mechanical perturbations. To do this we will fuse each sensor to the promiscuous biotin ligase protein BirA, and express the fusion proteins in cells under various conditions. The biotin ligase will label proteins in close proximity with biotin. Labeled proteins will be purified and analyzed by mass spectrometry, and the spectrum of interacting partners under different conditions will be compared.

    Collapse Post Docs

    A postdoctoral position is available to study in this laboratory. Contact Dr. McCollum for additional details.

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Ray S, DeSilva C, Dasgupta I, Mana-Capelli S, Cruz-Calderon N, McCollum D. The ability of the LIMD1 and TRIP6 LIM domains to bind strained f-actin is critical for their tension dependent localization to adherens junctions and association with the Hippo pathway kinase LATS1. Cytoskeleton (Hoboken). 2024 Mar 01. PMID: 38426816.
    2. Jebeli M, Lopez SK, Goldblatt ZE, McCollum D, Mana-Capelli S, Wen Q, Billiar K. Multicellular aligned bands disrupt global collective cell behavior. Acta Biomater. 2023 06; 163:117-130. PMID: 36306982.
      Citations: 2     Fields:    Translation:Humans
    3. Wang X, Zheng F, Yi YY, Wang GY, Hong LX, McCollum D, Fu C, Wang Y, Jin QW. Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress. Nat Commun. 2022 09 22; 13(1):5565. PMID: 36138017.
      Citations:    Fields:    Translation:AnimalsCells
    4. Goldblatt ZE, Ashouri Choshali H, Cirka HA, Liang V, Wen Q, McCollum D, Rahbar N, Billiar KL. Heterogeneity Profoundly Alters Emergent Stress Fields in Constrained Multicellular Systems. Biophys J. 2020 01 07; 118(1):15-25. PMID: 31812354.
      Citations: 4     Fields:    Translation:Cells
    5. Dasgupta I, McCollum D. Control of cellular responses to mechanical cues through YAP/TAZ regulation. J Biol Chem. 2019 11 15; 294(46):17693-17706. PMID: 31594864.
      Citations: 100     Fields:    Translation:HumansAnimalsCells
    6. Mana-Capelli S, McCollum D. Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling. J Biol Chem. 2018 11 23; 293(47):18230-18241. PMID: 30266805.
      Citations: 38     Fields:    Translation:HumansCells
    7. Dutta S, Mana-Capelli S, Paramasivam M, Dasgupta I, Cirka H, Billiar K, McCollum D. TRIP6 inhibits Hippo signaling in response to tension at adherens junctions. EMBO Rep. 2018 02; 19(2):337-350. PMID: 29222344.
      Citations: 29     Fields:    Translation:HumansCells
    8. Li Q, Li S, Mana-Capelli S, Roth Flach RJ, Danai LV, Amcheslavsky A, Nie Y, Kaneko S, Yao X, Chen X, Cotton JL, Mao J, McCollum D, Jiang J, Czech MP, Xu L, Ip YT. The conserved misshapen-warts-Yorkie pathway acts in enteroblasts to regulate intestinal stem cells in Drosophila. Dev Cell. 2014 Nov 10; 31(3):291-304. PMID: 25453828.
      Citations: 89     Fields:    Translation:AnimalsCells
    9. Gupta S, Govindaraghavan M, McCollum D. Cross talk between NDR kinase pathways coordinates cytokinesis with cell separation in Schizosaccharomyces pombe. Eukaryot Cell. 2014 Aug; 13(8):1104-12. PMID: 24972934.
      Citations: 10     Fields:    Translation:AnimalsCells
    10. Mana-Capelli S, Paramasivam M, Dutta S, McCollum D. Angiomotins link F-actin architecture to Hippo pathway signaling. Mol Biol Cell. 2014 May; 25(10):1676-85. PMID: 24648494.
      Citations: 114     Fields:    Translation:HumansCells
    11. Ma H, McLean JR, Gould KL, McCollum D. An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells. Methods Mol Biol. 2014; 1177:175-91. PMID: 24943323.
      Citations: 1     Fields:    Translation:HumansAnimals
    12. Bajpai A, Feoktistova A, Chen JS, McCollum D, Sato M, Carazo-Salas RE, Gould KL, Csik?sz-Nagy A. Dynamics of SIN asymmetry establishment. PLoS Comput Biol. 2013; 9(7):e1003147. PMID: 23874188.
      Citations: 7     Fields:    Translation:AnimalsCells
    13. Cipak L, Gupta S, Rajovic I, Jin QW, Anrather D, Ammerer G, McCollum D, Gregan J. Crosstalk between casein kinase II and Ste20-related kinase Nak1. Cell Cycle. 2013 Mar 15; 12(6):884-8. PMID: 23462181.
      Citations: 5     Fields:    Translation:AnimalsCells
    14. Gupta S, Mana-Capelli S, McLean JR, Chen CT, Ray S, Gould KL, McCollum D. Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Curr Biol. 2013 Feb 18; 23(4):333-8. PMID: 23394829.
      Citations: 26     Fields:    Translation:AnimalsCells
    15. McCollum D. Monopolin. Curr Biol. 2012 Nov 20; 22(22):R937-8. PMID: 23174290.
      Citations: 2     Fields:    Translation:AnimalsCells
    16. Mana-Capelli S, McLean JR, Chen CT, Gould KL, McCollum D. The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning. Mol Biol Cell. 2012 Dec; 23(23):4592-600. PMID: 23087209.
      Citations: 22     Fields:    Translation:AnimalsCells
    17. Wang Y, Li WZ, Johnson AE, Luo ZQ, Sun XL, Feoktistova A, McDonald WH, McLeod I, Yates JR, Gould KL, McCollum D, Jin QW. Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast. Mol Biol Cell. 2012 Sep; 23(17):3348-56. PMID: 22809626.
      Citations: 4     Fields:    Translation:AnimalsCells
    18. Johnson AE, McCollum D, Gould KL. Polar opposites: Fine-tuning cytokinesis through SIN asymmetry. Cytoskeleton (Hoboken). 2012 Oct; 69(10):686-99. PMID: 22786806.
      Citations: 48     Fields:    Translation:AnimalsCells
    19. Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D. A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms. Mol Cell Proteomics. 2012 Aug; 11(8):501-11. PMID: 22474084.
      Citations: 16     Fields:    Translation:HumansAnimalsCells
    20. Rentas S, Saberianfar R, Grewal C, Kanippayoor R, Mishra M, McCollum D, Karagiannis J. The SET domain protein, Set3p, promotes the reliable execution of cytokinesis in Schizosaccharomyces pombe. PLoS One. 2012; 7(2):e31224. PMID: 22347452.
      Citations: 10     Fields:    Translation:Animals
    21. Choi SH, McCollum D. A role for metaphase spindle elongation forces in correction of merotelic kinetochore attachments. Curr Biol. 2012 Feb 07; 22(3):225-30. PMID: 22264609.
      Citations: 17     Fields:    Translation:HumansAnimalsCells
    22. Gupta S, McCollum D. Crosstalk between NDR kinase pathways coordinates cell cycle dependent actin rearrangements. Cell Div. 2011 Nov 11; 6:19. PMID: 22079013.
    23. Paramasivam M, Sarkeshik A, Yates JR, Fernandes MJ, McCollum D. Angiomotin family proteins are novel activators of the LATS2 kinase tumor suppressor. Mol Biol Cell. 2011 Oct; 22(19):3725-33. PMID: 21832154.
      Citations: 114     Fields:    Translation:HumansCells
    24. McCollum D. Prevention of merotelic chromosome attachments by the monopolin complex. Cell Cycle. 2010 Nov 01; 9(21):4258. PMID: 21057196.
      Citations: 1     Fields:    Translation:AnimalsCells
    25. Ray S, Kume K, Gupta S, Ge W, Balasubramanian M, Hirata D, McCollum D. The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe. J Cell Biol. 2010 Sep 06; 190(5):793-805. PMID: 20805322.
      Citations: 25     Fields:    Translation:AnimalsCells
    26. McCollum D. Cytokinesis: ER keeps Mid1 in the middle. Curr Biol. 2010 Jun 08; 20(11):R484-6. PMID: 20541496.
      Citations: 2     Fields:    Translation:AnimalsCells
    27. Garc?a-Cort?s JC, McCollum D. Proper timing of cytokinesis is regulated by Schizosaccharomyces pombe Etd1. J Cell Biol. 2009 Sep 07; 186(5):739-53. PMID: 19736319.
      Citations: 33     Fields:    Translation:AnimalsCells
    28. Choi SH, P?li-Gulli MP, Mcleod I, Sarkeshik A, Yates JR, Simanis V, McCollum D. Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation. Curr Biol. 2009 Jun 23; 19(12):985-95. PMID: 19523829.
      Citations: 12     Fields:    Translation:AnimalsCells
    29. Chen CT, Feoktistova A, Chen JS, Shim YS, Clifford DM, Gould KL, McCollum D. The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1. Curr Biol. 2008 Oct 28; 18(20):1594-9. PMID: 18951025.
      Citations: 52     Fields:    Translation:AnimalsCells
    30. Yan H, Ge W, Chew TG, Chow JY, McCollum D, Neiman AM, Balasubramanian MK. The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast. Mol Biol Cell. 2008 Sep; 19(9):3676-90. PMID: 18562696.
      Citations: 10     Fields:    Translation:AnimalsCells
    31. Clifford DM, Chen CT, Roberts RH, Feoktistova A, Wolfe BA, Chen JS, McCollum D, Gould KL. The role of Cdc14 phosphatases in the control of cell division. Biochem Soc Trans. 2008 Jun; 36(Pt 3):436-8. PMID: 18481975.
      Citations: 17     Fields:    Translation:HumansAnimalsCells
    32. Jin QW, Ray S, Choi SH, McCollum D. The nucleolar Net1/Cfi1-related protein Dnt1 antagonizes the septation initiation network in fission yeast. Mol Biol Cell. 2007 Aug; 18(8):2924-34. PMID: 17538026.
      Citations: 8     Fields:    Translation:AnimalsCells
    33. Chen CT, Peli-Gulli MP, Simanis V, McCollum D. S. pombe FEAR protein orthologs are not required for release of Clp1/Flp1 phosphatase from the nucleolus during mitosis. J Cell Sci. 2006 Nov 01; 119(Pt 21):4462-6. PMID: 17032733.
      Citations: 10     Fields:    Translation:AnimalsCells
    34. Jin QW, Zhou M, Bimbo A, Balasubramanian MK, McCollum D. A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors. Genetics. 2006 Apr; 172(4):2101-12. PMID: 16415366.
      Citations: 26     Fields:    Translation:AnimalsCells
    35. McCollum D. Cytokinesis: breaking the ties that bind. Curr Biol. 2005 Dec 20; 15(24):R998-1000. PMID: 16360680.
      Citations: 2     Fields:    Translation:AnimalsCells
    36. Trautmann S, McCollum D. Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation. Curr Biol. 2005 Aug 09; 15(15):1384-9. PMID: 16085490.
      Citations: 19     Fields:    Translation:AnimalsCells
    37. Doxsey S, McCollum D, Theurkauf W. Centrosomes in cellular regulation. Annu Rev Cell Dev Biol. 2005; 21:411-34. PMID: 16212501.
      Citations: 139     Fields:    Translation:AnimalsCells
    38. McCollum D. Cytokinesis: the central spindle takes center stage. Curr Biol. 2004 Nov 23; 14(22):R953-5. PMID: 15556852.
      Citations: 19     Fields:    Translation:AnimalsCells
    39. Trautmann S, Rajagopalan S, McCollum D. The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase. Dev Cell. 2004 Nov; 7(5):755-62. PMID: 15525536.
      Citations: 27     Fields:    Translation:AnimalsCells
    40. Mishra M, Karagiannis J, Trautmann S, Wang H, McCollum D, Balasubramanian MK. The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe. J Cell Sci. 2004 Aug 01; 117(Pt 17):3897-910. PMID: 15265986.
      Citations: 51     Fields:    Translation:AnimalsCells
    41. Mitra P, Zhang Y, Rameh LE, Ivshina MP, McCollum D, Nunnari JJ, Hendricks GM, Kerr ML, Field SJ, Cantley LC, Ross AH. A novel phosphatidylinositol(3,4,5)P3 pathway in fission yeast. J Cell Biol. 2004 Jul 19; 166(2):205-11. PMID: 15249580.
      Citations: 33     Fields:    Translation:Animals
    42. Hou MC, Guertin DA, McCollum D. Initiation of cytokinesis is controlled through multiple modes of regulation of the Sid2p-Mob1p kinase complex. Mol Cell Biol. 2004 Apr; 24(8):3262-76. PMID: 15060149.
      Citations: 33     Fields:    Translation:AnimalsCells
    43. Jin QW, McCollum D. Scw1p antagonizes the septation initiation network to regulate septum formation and cell separation in the fission yeast Schizosaccharomyces pombe. Eukaryot Cell. 2003 Jun; 2(3):510-20. PMID: 12796296.
      Citations: 11     Fields:    Translation:AnimalsCells
    44. Guertin DA, Venkatram S, Gould KL, McCollum D. Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN). Dev Cell. 2002 Dec; 3(6):779-90. PMID: 12479804.
      Citations: 43     Fields:    Translation:AnimalsCells
    45. Guertin DA, Trautmann S, McCollum D. Cytokinesis in eukaryotes. Microbiol Mol Biol Rev. 2002 Jun; 66(2):155-78. PMID: 12040122.
      Citations: 112     Fields:    Translation:HumansAnimalsCells
    46. Hou MC, McCollum D. Cytokinesis: myosin spots the ring. Curr Biol. 2002 Apr 30; 12(9):R334-6. PMID: 12007437.
      Citations: 2     Fields:    Translation:Animals
    47. Wang H, Tang X, Liu J, Trautmann S, Balasundaram D, McCollum D, Balasubramanian MK. The multiprotein exocyst complex is essential for cell separation in Schizosaccharomyces pombe. Mol Biol Cell. 2002 Feb; 13(2):515-29. PMID: 11854409.
      Citations: 85     Fields:    Translation:AnimalsCells
    48. Trautmann S, Wolfe BA, Jorgensen P, Tyers M, Gould KL, McCollum D. Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression. Curr Biol. 2001 Jun 26; 11(12):931-40. PMID: 11448769.
      Citations: 92     Fields:    Translation:AnimalsCells
    49. McCollum D, Gould KL. Timing is everything: regulation of mitotic exit and cytokinesis by the MEN and SIN. Trends Cell Biol. 2001 Feb; 11(2):89-95. PMID: 11166217.
      Citations: 92     Fields:    Translation:AnimalsCells
    50. Sluder G, McCollum D. Molecular biology. The mad ways of meiosis. Science. 2000 Jul 14; 289(5477):254-5. PMID: 10917849.
      Citations: 4     Fields:    Translation:HumansAnimalsCells
    51. Hou MC, Salek J, McCollum D. Mob1p interacts with the Sid2p kinase and is required for cytokinesis in fission yeast. Curr Biol. 2000 May 18; 10(10):619-22. PMID: 10837231.
      Citations: 50     Fields:    Translation:AnimalsCells
    52. Guertin DA, Chang L, Irshad F, Gould KL, McCollum D. The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast. EMBO J. 2000 Apr 17; 19(8):1803-15. PMID: 10775265.
      Citations: 72     Fields:    Translation:AnimalsCells
    53. Sparks CA, Morphew M, McCollum D. Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis. J Cell Biol. 1999 Aug 23; 146(4):777-90. PMID: 10459013.
      Citations: 71     Fields:    Translation:AnimalsCells
    54. McCollum D, Balasubramanian M, Gould K. Identification of cold-sensitive mutations in the Schizosaccharomyces pombe actin locus. FEBS Lett. 1999 May 28; 451(3):321-6. PMID: 10371213.
      Citations: 7     Fields:    Translation:Animals
    55. B?hler J, Steever AB, Wheatley S, Wang Yl, Pringle JR, Gould KL, McCollum D. Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. J Cell Biol. 1998 Dec 14; 143(6):1603-16. PMID: 9852154.
      Citations: 140     Fields:    Translation:Animals
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