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Rotation Projects: Background for Student Rotation Projects <p >See Politz, Hogan and Pederson (2009) and Politz et al. (2005) in the above list. The novel microRNA work may be the most suitable for student rotations. Potential Rotation Projects <p >Are the nucleolar microRNAs pri-, pre- or mature? <p >Are they complexed with other nucleolar RNAs? <p >Do the cell cycle related proteins in the nucleolus exist as heterotypic complexes? <p >Does the nucleolus of human embryonic stem cells also share these features (which we have so far looked for, and found, only in transformed human cell lines)? <p >Can vanadium tagging be used to detect specific proteins by electron microscopy, perhaps exceeding the limitations of immuno-EM?
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Post Docs: Postdoctoral Fellow Applications are being accepted for one position. A position is available to develop Cys2His2 zinc finger proteins (ZFPs) for the treatment of monogenic disorders. This project will focus on the creation of ZFPs with novel DNA-binding specificity to recognize sequence elements within genomic loci of therapeutic interest. In collaboration with other laboratories, we will evaluate the activity of these ZFP-based gene therapy reagents in vitro and in vivo in relevant disease models. Applicants will also have the opportunity to learn to apply Cas9 and Cas12a editing systems in vivo.
Rotation Projects: Potential Rotation Projects Improving the Precision of CRISPR/Cas9 nucleases for gene therapy applications: We are working to improve the precision of the CRISPR/Cas9 system to generate nucleases that will cleave at only a single site in the genome. These engineering efforts focus on increasing the DNA-editing precision of Cas9 and using protein engineering to introduce new properties into the nuclease. Much of this work is now transitioning to the use of Cas9 (and Cas12a) protein-RNA complexes. These modified nucleases will then be applied to patient derived cell-culture systems for the targeted repair or inactivation of disease-causing alleles. With the eventual goal of creating therapeutics for Sickle Cell Disease, beta-Thalassemia, HIV, Limb Girdle Muscular Dystrophy, Hermansky Pudlak Syndrome and other monogenic disorders. ex vivo genome editing in CD34+ HSPCs: We are developing improved Cas9 proteins for delivery ex vivo into CD34+ HSPCs for therapeutic application to sickle cell disease and beta-thalassemia. The goal is to modify the hematopoietic stem cells of a patient to complement the loss of function of the beta-globin gene and then return these cells to the patient through an autologous transplant. Development of Cys2His2 Zinc fingers proteins (ZFPs) as targeted therapeutics: We are developing artificial ZFPs for the regulation of target genes to change their gene expression profiles for therapeutic applications.
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Ayers, David
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Lines, Lisa
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Vitello-Cicciu, Joan
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Kennedy, Daniel
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Driban, Jeffrey
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Moffatt-Bruce, Susan
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Mosenthal, Anne
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Medicine at UMass Chan - Lahey
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Surgery at UMass Chan - Lahey
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Chair and Professor
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Professor
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Professor
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https://profiles.umassmed.edu/profile/40402890
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Distinguished Professor and Chair Emeritus
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Breast Imaging
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Health System Science
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Pulmonary Medicine
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Professor
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Associate Professor
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Professor
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Professor
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Professor
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Professor
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Professor
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Assistant Professor
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Professor
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Professor
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Professor
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Chair and Professor
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Associate Professor
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Finlay, Andrea
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Geissler, Kimberley
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Ramsey, David
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Associate Professor
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Associate Professor
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Professor
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Tan Chingfen Graduate School of Nursing
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Professor
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Professor
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Gazarian, Priscilla
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Summary: Priscilla Gazarian, PhD CNS RN, is Professor of Nursing and Associate Dean for Academic Affairs in the Tan Chingfen Graduate School of Nursing.
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Professor
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Martinez, Fernando
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Endowed Chair Honorary Title: Joseph D. Early Chair in Biomedical Research
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Professor
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Professor Emeritus
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Mourtzinos, Arthur
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Person ID: 10247997
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Associate Professor
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Associate Professor
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Chair and Professor
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Abelson, Jonathan
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Person ID: 10248179
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Assistant Professor
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Associate Professor
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HR job title: Associate Professor
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Professor
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Professor Emeritus
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Assistant Professor
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Associate Professor
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Chair And Professor
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Professor Emeritus
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Psychiatry and Behavioral Sciences
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Professor
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Professor
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Associate Professor
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Adjunct Professor
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Professor
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Professor
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HR job title: Professor
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Adjunct Professor
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Professor
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Assistant Professor
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HR job title: Assistant Professor
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Professor Emeritus
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HR job title: Professor Emeritus
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https://profiles.umassmed.edu/profile/88604742
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Associate Professor
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HR job title: Associate Professor
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https://profiles.umassmed.edu/profile/96804229
number of subject areas: 4
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Viveiros, Jennifer
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Person ID: 10248660
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Assistant Professor
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