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Mello, Craig
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Rotation Projects: Potential Rotation Projects: There are numerous potential rotation project available in the Mello lab: Projects include: Investigation of RNAi and related mechanisms, including Piwi Argonaute pathways and their affects on fertility and epigenetic inheritance; Investigation of germline determination and cell-fate specification during embryogenesis; the exploration of CRISPR mediated genome editing tools for C. elegans and other organisms; and finally the use of CRISPR to generate C. elegans models for human genetic disease.
preferred title: Distinguished Professor
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Akalin, Ali
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Rando, Oliver
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Post Docs: For more information about the Postdoctoral position in Dr. Rando’s lab please use the link provided. Post doc position
Rotation Projects: Rotation projects are available to study chromatin structure and function. Project 1. Measure histone dynamics in yeast. Histone dynamics play major roles in chromatin metabolism and in the inheritance of chromatin states. To measure histone replacement rates in yeast, we have created yeast strains carrying inducible epitope-tagged histones H3 and H2A. Using microarrays, we will measure histone replacement rates in a variety of mutant yeast to ascertain the role of various chromatin remodeling factors in histone exchange reactions. Project 2. Characterize mechanisms for epigenetic inheritance in yeast. We have identified a variety of genes whose expression state in yeast is epigenetically heritable. We will screen the yeast genome for genes that affect the inheritance of these expression patterns. Project 3. Measure histone dynamics in embryonic stem cells. Embryonic stem cells are pluripotent cells that can give rise to every type of cell in the adult animal. The role of chromatin structure in maintaining this pluripotent state is poorly understood, but it appears that stem cell chromatin is exceptionally labile relative to that in differentiated cells. We will create stem cell lines with inducible tagged histones to measure histone replacement rates across the mouse genome.
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Weng, Zhiping
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Post Docs: The project aims to develop computational algorithms and software programs for analyzing high-throughput sequence data such as ChIP-Seq RNA-Seq small RNA sequencing data. PhD required. A strong background in computational biology especially experience with analyzing high-throughput sequencing data is highly desirable. Excellent communication and writing skills and the ability to work with experimental and computational collaborators are essential. To apply please forward CV cover letter and three references to: Zhiping.Weng@umassmed.edu
Rotation Projects: Please contact Dr. Weng for information on rotations that are available in her lab.
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Ambros, Victor
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Madoff, Lawrence
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Shaffer, Scott
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He, Feng
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Summary: Focus: Post-transcriptional gene regulation in eukaryotes
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Mandon, Elisabet
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Post Docs: <p >A postdoctoral research position is available to investigate Amyotrophic LateralSclerosis (ALS) mitochondrial dysfunction. ALS causes motor neurondegeneration, then paralysis and death. An early target in ALS is themitochondrion, whose dysfunction enhances ALS progression. The most commonALS-causing mutations are found in the copper-zinc superoxide dismutase (SOD1)gene. Most wild type (WT) SOD1 protein (SOD1WT) resides in the cytosol and only1% is targeted to the inner mitochondrial space (IMS). In contrast, mutant SOD1protein (SOD1mut) accumulates in the mitochondrial outer membrane and in thematrix, and subsequently impairs mitochondrial function by a mechanism that ispoorly understood. Abnormal SOD1mut accumulation leads to mitochondrialdysfunction found in the early stage of presymptomatic ALS mice, before theonset of motor neuron degeneration. Thus, it is critical to determine the firstevent induced by SOD1mut and devise a strategy to stop or delay it beforemitochondrial damage becomes irreversible. The objective of future studies willbe to determine which step/steps are affected by the SOD1 mutation byidentifying the sequence of events involved in SOD1mut and SOD1WT mitochondrialtargeting, import and IMS-sorting. This project will provide important basicinformation on the mechanisms underlying mitochondrial dysfunction in ALS andwill identify possible targets for future therapies. <p > Applicants must have (or expect toobtain shortly) a Ph.D. in Biochemistry, Cell Biology, Molecular Biology or arelated field and be strongly motivated to conduct research in an excitingresearch environment. <p >
Summary: Focus: Mitochondria and the endoplasmic reticulum
preferred title: Associate Professor

Lien, Egil
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Czech, Michael
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Endowed Chair Honorary Title: Isadore and Fannie Foxman Chair in Medical Research
Post Docs: A postdoctoral position is available to study in this laboratory. Contact Dr. Czech for additional details.
Rotation Projects: Mentoring……………………………………………………………………………………………………… The Czech laboratory is active in mentoring graduate students and postdoctoral fellows. Many former student and postdoctoral trainees of the Czech laboratory are now internationally recognized professors at major universities and medical schools. These former lab members include: Paul Pilch, Professor of Biochemistry, Boston University School of Medicine Jeffrey Pessin, Professor and Director of the Diabetes Center, Einstein College of Medicine Joan Massague, Director, Sloan Kettering Institute Roger Davis, HHMI investigator and Professor of Molecular Medicine, UMASS Medical School Silvia Corvera, Professor of Molecular Medicine, Co-Director of the MD/PhD Program, UMASS Medical School Carla Greenbaum, Vice Chair and Director of Clinical Research, Benaroya Research Institute Jes Klarlund, Professor of Ophthalmology, University of Pittsburgh Medical Center Rob Lewis, Professor of Biochemistry and Molecular Biology, Eppley Cancer Institute, University of Nebraska Medical Center Richard MacDonald, Professor of Biochemistry and Molecular Biology, Eppley Cancer Institute, University of Nebraska Medical Center Assia Shisheva, Professor of Physiology, Wayne State School of Medicine. Mark Sleeman, Professor of Physiology, Monash University, Australia John Harris, Associate Professor of Medicine, UMASS Medical School Zhen Jiang, Associate Professor of Pharmacology and Medicine, Boston University Vishu Puri, Professor of Biomedical Sciences, Ohio University Olga Gupta, Assistant Professor of Pediatrics and Medicine, University of Texas Southwestern Medical Center Tim Fitzgibbons, Assistant Professor of Medicine, UMASS Medical School Myriam Aouadi, Assistant Professor, Integrated Cardio Metabolic Centre, Karolinska Institute Adilson Guilherme, Associate Professor, University of Massachusetts Medical School Our laboratory currently offers graduate student Rotation Projects (examples below and others available) and Thesis Research …
preferred title: Professor Emeritus

Munson, Mary
first name: Mary
last name: Munson
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Rotation Projects: Potential Rotation Projects <p >Research in the Munson lab is focused on biochemical/biophysical and cell biological characterization of proteins in the exocyst complex. Potential rotation projects include the following: Cloning of various exocyst protein domains and point mutations. These will be expressed in E. coli for biochemical/structural studies, and their functions tested in yeast. Protein expression and purification. Develop purification strategies for several exocyst proteins and their domains, using chromatography methods such as ion exchange and gel filtration (Fig. 2). Characterization of the purified exocyst proteins. Protein structure, stability, oligomerization state and protein:protein interactions will be monitored by such techniques as circular dichroism, analytical ultracentrifugation and gel filtration (Fig. 3). Crystallography. We have determined the structure of the C-terminal domain of Sec6p (Fig. 4). Crystallization trials ofotherexocyst proteins and their domains are in progress. Design and test functional exocyst mutants in yeast. Mutants will be characterized using a variety of biochemical, cell biological, and microscopic techniques. Identify novel regulators of yeast exocytosis using a genetic screen. Mutants created in these screens are currently being tested and identified (Fig. 5). Their role in exocytosis will be explored <p >
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Rhind, Nicholas
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Rotation Projects: Potential Rotation Projects 1) Coordination of replication and recombination by the S-phase DNA damage checkpoint The replication checkpoint coordinates replication and recombinational repair to allow for the replication of damaged templates, however the mechanism by which the checkpoint regulates recombination is unknown. We have identified a number of potential checkpoint-kinase targets by phosphoproteomics. One possible rotation project would be to create site-directed mutations that would prevent kinase regulation of one or more of the targets. These mutations could be used to validate the role of the target in the checkpoint and to test the importance of checkpoint regulation in S-phase DNA repair. 2) Regulation of replication timing Heterochromatin regulates the timing of replication origin firing, however the mechanism by which it affects origin function is unknown. We have developed a model of how origin timing is regulated in non-heterochromatic chromatin; now we want to test how heterochromatin affects this mechanism. In particular, we want to manipulate the heterochromatic context at specific loci, measure the changes in origin timing and investigate the molecular mechanisms responsible for the change. One possible rotation project would be to establish heterochromatin at a well-characterized origin, measure origin timing, which we presume will be delayed, and then test at which step origin firing is delayed. <p >
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Strambio De Castillia, Caterina
first name: Caterina
last name: Strambio De Castillia
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Summary: Dr. Strambio-De-Castillia (ORCID: https://orcid.org/0000-0002-1069-1816) is an internationally recognized cell biologist and imaging scientist who specializes in developing easy-to-use and openly accessible strategies for image data management and sharing to promote scientific discovery in health and disease.
preferred title: Assistant Professor
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Golenbock, Douglas
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Haran, John
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Rotation Projects: Rotation projects are available related to microbiota-gut brain axis and antibiotic usage and the sequelae of antibiotic use in hospitalized patients which include Clostridium difficile and multi-drug resistant organism infection and colonization.
preferred title: Professor

Gierasch, Lila
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Schwartz, Lawrence
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Rotello, Vincent
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Maroney, Michael
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McClements, David
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Blanchard, Jeffrey
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Riley, Margaret
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Rich, Stephen
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Xiao, Hang
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Chien, Peter
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Ma, Li-Jun
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Rogaev, Evgeny
first name: Evgeny
last name: Rogaev
full name: Evgeny I Rogaev PhD
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Person ID: 39
person in primary position: Professor
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Post Docs: A postdoctoral position is available to study in this laboratory. Contact Dr. Rogaev for additional details.
preferred title: Professor

Baker, Richard
first name: Richard
last name: Baker
full name: Richard E Baker PhD
has faculty rank: Associate Professor
latitude: 42.27525000000000
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Person ID: 486
person in primary position: Associate Professor
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preferred title: Associate Professor

Zitzewitz, Jill
first name: Jill
last name: Zitzewitz
full name: Jill A Zitzewitz PhD
has faculty rank: Associate Professor
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person in primary position: Associate Professor
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preferred title: Associate Professor

McCollum, Dannel
first name: Dannel
last name: McCollum
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Person ID: 561
person in primary position: Professor
type: Agent
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Post Docs: A postdoctoral position is available to study in this laboratory. Contact Dr. McCollum for additional details.
Rotation Projects: Rotation Projects Analog sensitive LATS kinase: The goal of this project is to use CRISPR based genome modification to make mutations in the endogenous LATS kinase that cause it to be inhibited by a synthetic ATP analog. This will allow LATS kinase activity to be turned on and off using the ATP analog. The analog sensitive cell line will be a valuable tool for studying Hippo pathway responses to various stimuli and will be used to test whether dephosphorylation of LATS substrates is an important regulatory mechanism. This project will also set the stage for future experiments to create mice that have analog sensitive versions of LATS. These mice will be used to test whether LATS inhibition is a viable treatment for regenerative therapies for the heart, gut, pancreas and other organs. TRIP6 and Angiomotin interacting proteins: We know that cells monitor their mechanical environment by sensing F-actin levels and by measuring tension at cell-cell junctions. We have identified angiomotins as sensors for F-actin and the TRIP6 protein as part of a tension sensor at cell-cell junctions. However, to understand how these sensors work, we need know the network of proteins that they each interact with, and how those networks change in response to mechanical perturbations. To do this we will fuse each sensor to the promiscuous biotin ligase protein BirA, and express the fusion proteins in cells under various conditions. The biotin ligase will label proteins in close proximity with biotin. Labeled proteins will be purified and analyzed by mass spectrometry, and the spectrum of interacting partners under different conditions will be compared.
preferred title: Professor

Wiss, Karen
first name: Karen
last name: Wiss
full name: Karen Wiss MD
has faculty rank: Professor Emeritus
latitude: 42.28554670000000
longitude: -71.79046720000000
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Person ID: 599
person in primary position: Professor Emeritus
type: Agent
type: Person
Summary: Interests: Pediatric dermatology, epidermolysis bullosa, genodermatoses, midline developmental defects, childhood exanthems, medical education, pediatric laser
preferred title: Professor Emeritus

Peterson, Craig
first name: Craig
last name: Peterson
full name: Craig L Peterson PhD
has faculty rank: Professor
latitude: 42.27913100000000
longitude: -71.76550320000000
Person ID: 633
person in primary position: Professor
type: Agent
type: Person
Endowed Chair Honorary Title: Elisabeth Chair I
preferred title: Professor

Ruiz De Luzuriaga, Katherine
first name: Katherine
last name: Ruiz De Luzuriaga
full name: Katherine Ruiz De Luzuriaga MD
has faculty rank: Professor
latitude: 42.27913100000000
longitude: -71.76550320000000
Person ID: 651
person in primary position: Professor
type: Agent
type: Person
Endowed Chair Honorary Title: UMass Memorial Health Care Chair in Biomedical Research
Rotation Projects: Potential Rotation Projects: Contact Dr. Luzuriaga for project details.
preferred title: Professor

Pryciak, Peter
first name: Peter
last name: Pryciak
full name: Peter M Pryciak PhD
has faculty rank: Associate Professor
latitude: 42.27525000000000
longitude: -71.76233800000000
Person ID: 654
person in primary position: Associate Professor
type: Agent
type: Person
Rotation Projects: Rotation Projects Project #1: Signaling in the MAP kinase cascade. Membranerecruitment ofthe kinase cascade "scaffold" protein, Ste5, initiates signaling. But our recent work suggests that membrane localization alsoboosts signaling efficiency through the kinase cascade, and similar findings have been reported in a mammalian pathway. Yet the mechanism explaining this effect is unknown.This project would use molecular genetic approaches to probe thisphenomenon. Project #2: Membrane targeting domains. Our recent work identified a small membrane-binding domain that guides Ste5 to the plasma membrane, but it does so only acting in conjunction with protein-protein interactions. Relatedly, we have recently found similar domains, and their cooperative relationship with protein-protein interactions, in several other plasma membrane-localized signaling proteins. We suspect this may be a very common arrangement, and that multiple weak interactions are advantageous to ensuring dynamic localization that constantly probes the availability of membrane-localized binding partners. This project would involve conducting a small-scale survey of polarized proteins in yeast, searching for small membrane-binding domains that are critical for their normal localization and function.
preferred title: Associate Professor

Gilmore, James Reid
first name: James Reid
last name: Gilmore
full name: James Reid Gilmore PhD
has faculty rank: Professor Emeritus
latitude: 42.27985300000000
longitude: -71.79190270000000
Person ID: 669
person in primary position: Professor Emeritus
type: Agent
type: Person
Rotation Projects: Rotation Project Background
Rotation Projects: In yeast cells, protein translocation across the endoplasmic reticulum can occur by cotranslational or posttranslational pathways. The objective of this project is to develop a rapid method to inactivate the posttranslational pathway in vivo in yeast cells by appending the CMV-ribosome stalling sequence to the C-terminus of a typical posttranslational translocation substrate (carboxypeptidase Y). Complexes between the posttranslational translocation channel (SEC complex) and the stalled ribosomes will be purified for structural and functional analysis.
preferred title: Professor Emeritus

Watanabe, Shinya
first name: Shinya
last name: Watanabe
full name: Shinya Watanabe PhD
has faculty rank: Assistant Professor
latitude: 42.27932700000000
longitude: -71.76549170000000
Person ID: 700
person in primary position: Assistant Professor
type: Agent
type: Person
Post Docs: Postdoc position is available. Please contact us.
preferred title: Assistant Professor

Dekker, Job
first name: Job
last name: Dekker
full name: Job Dekker PhD
has faculty rank: Professor
latitude: 42.27587600000000
longitude: -71.76211600000000
Person ID: 759
person in primary position: Professor
type: Agent
type: Person
Endowed Chair Honorary Title: Joseph J. Byrne Chair in Biomedical Research
Rotation Projects: ? Rotation Projects Multiple rotation projects are available in several areas related to long-range gene regulation and higher order chromosome organization. Projects include thorough analyses of the biochemical mechanisms of long-range gene regulation of specific disease-related genes, as well as large-scale analyses of chromosome organization using high-throughput genomics technologies (5C and Hi-C). The lab employs a wide range of experimental approaches, including cell culture, protein biochemistry, microscopy, genomics as well computational approaches such as bioinformatics and modeling. Specific rotation projects are determined dependent on the interest of the student.
preferred title: Professor
webpage: https://profiles.umassmed.edu/profile/28866525
webpage: https://profiles.umassmed.edu/profile/28866572

Sassetti, Christopher
first name: Christopher
last name: Sassetti
full name: Christopher M Sassetti PhD
has faculty rank: Professor
latitude: 42.27587600000000
longitude: -71.76211600000000
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Person ID: 878
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Rotation Projects: Rotation Projects <p >We have identified a number of bacterial pathways that are required for bacterial viability or virulence. We are particularly interested in: Phosphosignaling systems that regulate cell wall synthesis Metabolic regulatory mechanisms that trigger the “dormancy” response observed during infection. <p >A variety of genetic, biochemical, and informatic methods will be used to define the functions of these genes and the role played by each during infection. <p >
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Wang, Yong-Xu
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Kaufman, Paul
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Ostroff, Gary
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Bolon, Daniel
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Rotation Projects: Potential Rotation Projects <p >Our laboratory combines genetic and biochemical approaches to investigate the molecular underpinnings of adaptation. Potential rotation projects are available in two general (and partially overlapping) areas: exploring fitness landscapes through systematic approaches and investigating the molecular mechanism of the Hsp90 chaperone in signal transduction. Please contact the lab to discuss specific rotation projects in more detail.
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Ram, Sanjay
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Artenstein, Andrew
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Research
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UMass Chan – Baystate Regional Campus
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Haynes, Cole
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Dermatology
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E&R Isenberg Prof Intg Env Sci
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Professor
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Fergus Clydesdale Endowed Chair
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Associate Professor
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Professor
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Assistant Professor
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Ito, Christopher
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ADJUNCT ASSISTANT PROFESSOR
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Kelleher, Daniel
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Robertson, David
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Population and Quantitative Health Sciences
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Bucci, Vanni
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Biochemistry and Molecular Biotechnology
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Medicine at UMass Chan - Baystate
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Pediatrics at UMass Chan - Baystate
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Professor Emeritus
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Assistant Professor
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Professor
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Assistant Professor
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Assistant Professor
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Professor
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Associate Professor
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Professor
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Mortzfeld, Benedikt
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Professor
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Professor
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Associate Professor
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Associate Professor
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Genomics and Computational Biology
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Restrepo, Angela
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Medicine at UMass Chan - Lahey
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Infectious Diseases
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Translational Anatomy
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Professor
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Professor
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Professor
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Professor Emeritus
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Professor
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Professor
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Professor
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Professor
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Professor
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Professor
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Chair and Professor
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Professor Emeritus
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Professor
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Microbiology
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Associate Professor
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Associate Professor
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Professor
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Professor
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Professor
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Professor
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Professor
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Professor
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Gordan, Raluca
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Psychiatry and Behavioral Sciences
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ProfilesRNS Plugin Searchable Data: FocalPlane features... data managent webinar Presentation at the BINA 2024 Community Congress FoundingGIDE: BioImage DMS for Everyone
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ProfilesRNS Plugin Searchable Data: FocalPlane features... data managent webinar FoundingGIDE: BioImage DMS for Everyone Presentation at the BINA 2024 Community Congress OME 2025 | Session 1 - Pre-publication BioImage DMS
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Associate Professor
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Distinguished Professor
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Professor
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Professor
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Carneiro, Herman
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Assistant Professor
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