The lab is currently looking for motivated postdoctoral scholars with backgrounds in genomics and computational biology. Please email the PI.
Rotation projects are available related to malaria and Burkitt lymphoma. Our projects range from genetic epidemiology to directed genomics studying human, parasite and tumor.
<p >Potential rotation projects My lab primarily investigates Plasmodium falciparum malaria-induced dysregulation of EBV-specific T cell immunity and its role in the etiology of endemic Burkitt lymphoma (eBL), the most prevalent pediatric cancer in Equatorial Africa. Within the context of our ongoing studies of healthy Kenyan children with divergent malaria exposure histories (chronic/repeated infections in a holoendemic area versus low infection rates in a nearby highland, malaria hypoendemic area) and by examining children diagnosed with eBL we have established a repository of samples that can be used to address this question. <p >1. One avenue of exploration involves homeostatic cytokine regulation of memory T cells. IL-15 and/or IL-7 responsiveness of EBV-specific memory T cells may be impaired in children with chronic malaria exposure and in children with endemic Burkitt lymphoma (eBL). We would like to investigate IL-15Ra and IL-7Ra surface expression, and CFSE proliferation of EBV-specific T cells in response to cognate antigen with and without IL-15 or IL-7 stimulation and compared children with divergent malaria exposure histories and children diagnosed with eBL. T cell responsiveness to IL-15 and IL-7 can be measured by STAT5 phosphorylation and the specificity of this effect for EBV-specific T cells can be evaluated by comparing IL-15Ra and IL-7Ra expression and responsiveness on other lymphocyte subsets and CMV-specific T cells. <p >2. Another putative mechanism by which malaria may interfere with the development of immunologic memory and influence EBV-specific T cell immunity is through the Programmed death-1 (PD-1) pathway. PD-1 is an immune inhibitory molecule that negatively regulates activated immune cells upon interacting with its ligands, programmed death ligand-1 (PD-L1) and programmed death ligand-2 (PD-L2) resulting in down-regulation of immune responses. Previous studies in murine and primate viral and parasitic diseases have reported the up-regulation of PD-1 and soluble PD-1 (sPD-1) but no studies have reported the expression of PD-1 in individuals from areas with divergent malaria transmission dynamics or in children presenting with endemic Burkitt lymphoma (eBL). In addition the PD-1 pathway has been suggested to be involved in the evasion of antitumor immunity by the host immune system. Preliminary studies in my lab suggest that PD-1 expression is significantly increased on CD4, CD8 T cells, B cells and NK cells in those residing in high malaria transmission areas compared to age-matched individuals without a history of malaria exposure. Future studies will explore the kinetics of this effect, the impact of antigen dose and how malaria exploits PD-1 mediated regulation to impair the development of immunologic memory. <p >3. Another area of interest is the role of Th17 cells in pediatric immunity. Because of the naturally delayed production of TH1 mediated pro-inflammatory cytokines (i.e. IL-12 and IFN-g) in utero, neonatal immunity is thought to be Ã¢â‚¬ËœsuppressedÃ¢â‚¬â„¢. However, more recent studies have shown that neonatal monocytes and antigen-presenting cells do express pro-inflammatory and regulatory cytokines (i.e. IL-6, IL-10 and IL-23) and at higher levels than adults. Thus TH17 cells appear to be an early, sequential response to pathogens prior to the induction of a TH1 or TH2 response. TH17 cells are also stimulated with IL-21 and IL-22 in an autocrine and paracrine fashion by natural killer cells. Exploring Th17 cell responses within the context of early-age human infections with malaria and EBV would be an exciting new avenue of investigation in my laboratory.
Fogwe L, Ganipisetti VM, Naha K. Brown Tumor From Secondary Hyperparathyroidism Mimicking Metastatic Renal Cell Carcinoma in a Patient With End-Stage Renal Disease. Cureus. 2024 Apr; 16(4):e59376.
Brown Tumor From Secondary Hyperparathyroidism Mimicking Metastatic Renal Cell Carcinoma in a Patient With End-Stage Renal Disease.
Ganipisetti VM, Maringanti BS, Lingas EC, Naha K
Ganipisetti VM, Maringanti BS, Lingas EC, Naha K. Adult Vitamin B12 Deficiency-Associated Pseudo-Thrombotic Microangiopathy: A Systematic Review of Case Reports. Cureus. 2024 Mar; 16(3):e55784.
Adult Vitamin B12 Deficiency-Associated Pseudo-Thrombotic Microangiopathy: A Systematic Review of Case Reports.
Parikh MP, Samo S, Ganipisetti V, Krishnan S, Dhandha M, Yungbluth M, Glaws WR
Parikh MP, Samo S, Ganipisetti V, Krishnan S, Dhandha M, Yungbluth M, Glaws WR. Gastric metastasis of Merkel cell carcinoma, a rare cause of gastrointestinal bleeding: case report and review of the literature. J Gastrointest Oncol. 2014 Aug; 5(4):E68-72.
Gastric metastasis of Merkel cell carcinoma, a rare cause of gastrointestinal bleeding: case report and review of the literature.
Samo S, Parikh M, Sheth J, Krishnan S, Ganipisetti V
QJM : monthly journal of the Association of Physicians
Samo S, Parikh M, Sheth J, Krishnan S, Ganipisetti V. Treatment of methemoglobinemia resulting in green urine. QJM. 2014 Dec; 107(12):1039.
Treatment of methemoglobinemia resulting in green urine.
Fuest S, Ganipisetti V, Cunningham JM. EBM BLS: Adding tirzepatide to long-acting insulin is superior to adding mealtime insulin for type 2 diabetes mellitus. J Gen Intern Med. 2024 Nov; 39(14):2878-2879.
EBM BLS: Adding tirzepatide to long-acting insulin is superior to adding mealtime insulin for type 2 diabetes mellitus.
Ganipisetti VM, Dudiki N, Athavale A, Bollimunta P, Maringanti BS
Ganipisetti VM, Dudiki N, Athavale A, Bollimunta P, Maringanti BS. Daptomycin-Rifampin-Induced Rhabdomyolysis, Acute Renal Failure, and Hepatic Injury: A Case Report and Literature Review. Cureus. 2023 Mar; 15(3):e36834.
Daptomycin-Rifampin-Induced Rhabdomyolysis, Acute Renal Failure, and Hepatic Injury: A Case Report and Literature Review.
Anil V, Ganipisetti V, Harisinghani K, Kanugula AK
Anil V, Ganipisetti V, Harisinghani K, Kanugula AK. Hairy Cell Leukemia (HCL) Presenting with Joint Swelling: Case Report and Literature Review of a Rare Rheumatological Manifestation of a Hematological Disease. Cureus. 2023 Mar; 15(3):e35912.
Hairy Cell Leukemia (HCL) Presenting with Joint Swelling: Case Report and Literature Review of a Rare Rheumatological Manifestation of a Hematological Disease.
Parikh MP, Krishnan S, Ganipisetti VM, Flores S
Parikh MP, Krishnan S, Ganipisetti VM, Flores S. Classic image: peripheral blood smear in a case of Plasmodium falciparum cerebral malaria. BMJ Case Rep. 2014 Sep 19; 2014.
Classic image: peripheral blood smear in a case of Plasmodium falciparum cerebral malaria.
Ganipisetti VM, Bollimunta P, Maringanti S
Ganipisetti VM, Bollimunta P, Maringanti S. Paxlovid-Induced Symptomatic Bradycardia and Syncope. Cureus. 2023 Jan; 15(1):e33831.
Paxlovid-Induced Symptomatic Bradycardia and Syncope.
Ganipisetti VM, Bollimunta P, Tun NN, Kanugula A, Anil V, Athavale A, Maringanti BS
Ganipisetti VM, Bollimunta P, Tun NN, Kanugula A, Anil V, Athavale A, Maringanti BS. Concomitant Vitamin B1 and Vitamin B12 Deficiency Mimicking Thrombotic Thrombocytopenic Purpura. Cureus. 2023 Jan; 15(1):e34421.
Concomitant Vitamin B1 and Vitamin B12 Deficiency Mimicking Thrombotic Thrombocytopenic Purpura.
Ganipisetti VM, Bollimunta P, Dudiki N. Spontaneous Biloma With a Hepatic Mass. Cureus. 2023 Jun; 15(6):e40249.
Lingas EC, Ganipisetti VM. Adult Hospitalists' Knowledge of Radiation Oncology in an Independent Nonacademic Healthcare System in Albuquerque, New Mexico. Cureus. 2023 Jul; 15(7):e41856.
Adult Hospitalists' Knowledge of Radiation Oncology in an Independent Nonacademic Healthcare System in Albuquerque, New Mexico.
Dudiki N, Ganipisetti VM, Kolli S, Thapa SS
Dudiki N, Ganipisetti VM, Kolli S, Thapa SS. A Large Lung Abscess in an Electronic Cigarette User: To Drain or Not to Drain. Cureus. 2023 Apr; 15(4):e37690.
A Large Lung Abscess in an Electronic Cigarette User: To Drain or Not to Drain.
Ganipisetti VM, Dudiki N, Athavale A. A Diagnostic Quandary of Escherichia Coli Pneumonia: A Case Report and Literature Review. Cureus. 2023 May; 15(5):e39668.
A Diagnostic Quandary of Escherichia Coli Pneumonia: A Case Report and Literature Review.
Maringanti BS, Ganipisetti VM, Jun SS, Flores MI
Maringanti BS, Ganipisetti VM, Jun SS, Flores MI. Tigecycline Tango: A Case of Antibiotic-Induced Pancreatitis. Cureus. 2023 Sep; 15(9):e44538.
Tigecycline Tango: A Case of Antibiotic-Induced Pancreatitis.
Parikh MP, Sherid M, Ganipisetti V, Gopalakrishnan V, Habib M, Tripathi M
Parikh MP, Sherid M, Ganipisetti V, Gopalakrishnan V, Habib M, Tripathi M. Vomiting-induced gastric emphysema and hepatoportal venous gas: a case report and review of the literature. Case Rep Med. 2015; 2015:413230.
Vomiting-induced gastric emphysema and hepatoportal venous gas: a case report and review of the literature.