nef Gene Products, Human Immunodeficiency Virus
Rotation Projects Rotation projects are available in support of our research. See the research description section of this page.
David J. Freelander Memorial Professor in AIDS Research
Worcester Foundation for Biomedical Research Chair III
Please email Kate.Fitzgerald@umassmed.edu if you are interested in rotations.
Arthur F. and Helen P. Koskinas Professor in Biochemistry and Molecular Biotechnology
UMass Memorial Health Care Chair in Biomedical Research
Potential Rotation Projects: Contact Dr. Luzuriaga for project details.
A postdoctoral position is available to study in this laboratory. Contact Dr. Gottlinger for additional details.
Potential Rotation Projects 1.The identification of novel binding partners for cellular proteins required for HIV-1 budding using a double tag immunoaffinity purification approach. 2.The characterization of mutant ESCRT-III components that block HIV-1 budding with regard to subcellular localization and their ability to interact with certain phosphoinositol lipids and with other proteins involved in late endosomal sorting. 3.The mapping of binding sites for cellular proteins that we have identified as specific components of HIV-1 using GST pulldown, yeast two-hybrid, and co-immunoprecipitation approaches. 4.The generation and functional characterization of mutant viral Gag proteins that fail to interact with specific cellular partners.
Usami Y, Wu Y, G?ttlinger HG. SERINC3 and SERINC5 restrict HIV-1 infectivity and are counteracted by Nef. Nature. 2015 Oct 08; 526(7572):218-23.
SERINC3 and SERINC5 restrict HIV-1 infectivity and are counteracted by Nef.
Fudan University Shanghai Medical College
Fudan University Shanghai Medical College,
Fudan University Shanghai Medical College, Immunology
Pasteur Institute, Biology & Biotechnology
Dai W, Usami Y, Wu Y, G?ttlinger H. A Long Cytoplasmic Loop Governs the Sensitivity of the Anti-viral Host Protein SERINC5 to HIV-1 Nef. Cell Rep. 2018 01 23; 22(4):869-875.
A Long Cytoplasmic Loop Governs the Sensitivity of the Anti-viral Host Protein SERINC5 to HIV-1 Nef.
Wu Y, Olety B, Weiss ER, Popova E, Yamanaka H, G?ttlinger H
Wu Y, Olety B, Weiss ER, Popova E, Yamanaka H, G?ttlinger H. Potent Enhancement of HIV-1 Replication by Nef in the Absence of SERINC3 and SERINC5. mBio. 2019 06 11; 10(3).
Potent Enhancement of HIV-1 Replication by Nef in the Absence of SERINC3 and SERINC5.
Popov S, Popova E, Inoue M, Wu Y, G?ttlinger H
Proceedings of the National Academy of Sciences of the United States of America
Popov S, Popova E, Inoue M, Wu Y, G?ttlinger H. HIV-1 gag recruits PACSIN2 to promote virus spreading. Proc Natl Acad Sci U S A. 2018 07 03; 115(27):7093-7098.
HIV-1 gag recruits PACSIN2 to promote virus spreading.
Kaushik R, Zhu X, Stranska R, Wu Y, Stevenson M
Kaushik R, Zhu X, Stranska R, Wu Y, Stevenson M. A cellular restriction dictates the permissivity of nondividing monocytes/macrophages to lentivirus and gammaretrovirus infection. Cell Host Microbe. 2009 Jul 23; 6(1):68-80.
A cellular restriction dictates the permissivity of nondividing monocytes/macrophages to lentivirus and gammaretrovirus infection.
Sharova N, Wu Y, Zhu X, Stranska R, Kaushik R, Sharkey M, Stevenson M
Sharova N, Wu Y, Zhu X, Stranska R, Kaushik R, Sharkey M, Stevenson M. Primate lentiviral Vpx commandeers DDB1 to counteract a macrophage restriction. PLoS Pathog. 2008 May 02; 4(5):e1000057.
Primate lentiviral Vpx commandeers DDB1 to counteract a macrophage restriction.
Biochemistry and Molecular Biotechnology
Olety B, Peters P, Wu Y, Usami Y, G?ttlinger H
Olety B, Peters P, Wu Y, Usami Y, G?ttlinger H. HIV-1 propagation is highly dependent on basal levels of the restriction factor BST2. Sci Adv. 2021 Oct 29; 7(44):eabj7398.
HIV-1 propagation is highly dependent on basal levels of the restriction factor BST2.
Olety B, Usami Y, Wu Y, Peters P, G?ttlinger H
Olety B, Usami Y, Wu Y, Peters P, G?ttlinger H. AP-2 Adaptor Complex-Dependent Enhancement of HIV-1 Replication by Nef in the Absence of the Nef/AP-2 Targets SERINC5 and CD4. mBio. 2023 02 28; 14(1):e0338222.
AP-2 Adaptor Complex-Dependent Enhancement of HIV-1 Replication by Nef in the Absence of the Nef/AP-2 Targets SERINC5 and CD4.
Olety B, Usami Y, Peters P, Wu Y, G?ttlinger H
Olety B, Usami Y, Peters P, Wu Y, G?ttlinger H. The ectodomain sheddase ADAM10 restricts HIV-1 propagation and is counteracted by Nef. Sci Adv. 2025 Apr 18; 11(16):eadt1836.
The ectodomain sheddase ADAM10 restricts HIV-1 propagation and is counteracted by Nef.