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Post Docs: Postdoctoral Fellow Applications are being accepted for one position. A position is available to develop Cys2His2 zinc finger proteins (ZFPs) for the treatment of monogenic disorders. This project will focus on the creation of ZFPs with novel DNA-binding specificity to recognize sequence elements within genomic loci of therapeutic interest. In collaboration with other laboratories, we will evaluate the activity of these ZFP-based gene therapy reagents in vitro and in vivo in relevant disease models. Applicants will also have the opportunity to learn to apply Cas9 and Cas12a editing systems in vivo.
Rotation Projects: Potential Rotation Projects Improving the Precision of CRISPR/Cas9 nucleases for gene therapy applications: We are working to improve the precision of the CRISPR/Cas9 system to generate nucleases that will cleave at only a single site in the genome. These engineering efforts focus on increasing the DNA-editing precision of Cas9 and using protein engineering to introduce new properties into the nuclease. Much of this work is now transitioning to the use of Cas9 (and Cas12a) protein-RNA complexes. These modified nucleases will then be applied to patient derived cell-culture systems for the targeted repair or inactivation of disease-causing alleles. With the eventual goal of creating therapeutics for Sickle Cell Disease, beta-Thalassemia, HIV, Limb Girdle Muscular Dystrophy, Hermansky Pudlak Syndrome and other monogenic disorders. ex vivo genome editing in CD34+ HSPCs: We are developing improved Cas9 proteins for delivery ex vivo into CD34+ HSPCs for therapeutic application to sickle cell disease and beta-thalassemia. The goal is to modify the hematopoietic stem cells of a patient to complement the loss of function of the beta-globin gene and then return these cells to the patient through an autologous transplant. Development of Cys2His2 Zinc fingers proteins (ZFPs) as targeted therapeutics: We are developing artificial ZFPs for the regulation of target genes to change their gene expression profiles for therapeutic applications.
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Gounis, Matthew
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Politi, Letterio
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Post Docs: Jungmin Kim
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Lindenauer, Peter
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Hemond, Christopher
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Rotation Projects: There is a current project opening for using deep learning to train and ultimately perform automatic segmentation for multiple brain structures of interest in Multiple Sclerosis. Helpful background would include some proficiency in shell and Python. Please email me if interested.
Summary: Dr. Hemond is a board-certified neurologist with clinical subspecialty training in neuroimmunology and multiple sclerosis. His research focuses on the development and validation of MRI-based biomarkers to improve (1) early identification and (2) pathophysiological understanding of the neurodegenerative processes underlying MS.
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Garwood, Elisabeth
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Usmani, Saad
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Erbay, Sami
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Shazeeb, Mohammed
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Bloch, Boris
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Medicine at UMass Chan - Baystate
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Saranathan, Manojkumar
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Vachha, Behroze
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Driban, Jeffrey
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Walkey, Allan
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Medicine at UMass Chan - Lahey
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Surgery at UMass Chan - Lahey
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https://profiles.umassmed.edu/profile/40402890
ProfilesRNS Plugin Searchable Data: Preventing Osteoarthritis After an ACL Injury
ProfilesRNS Plugin Searchable Data: Preventing Osteoarthritis After an ACL Injury Playing the Long Game: Consensus for Preventing OA
ProfilesRNS Plugin Searchable Data: Preventing Osteoarthritis After an ACL Injury Playing the Long Game: Consensus for Preventing OA Characteristics of Accelerated Hand Osteoarthritis

Radiology at UMass Chan - Lahey
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Health System Science
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Rassi, Jonathan
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