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Structure and Function of the Ribosome

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Dr.  Andrei Korostelev, Ph.D.

Ribosomes, the ancient and universal cellular machines, are responsible for decoding genetic information and synthesizing proteins in all living organisms. A eubacterial ribosome has a molecular weight of approximately 2.5 MDa and consists of about 55 proteins and 3 large RNA molecules. So far, the ribosome is the largest asymmetric macromolecule amenable to crystallographic analyses.

The multi-step process of translation is not fully understood: only recently, structural details of some steps of translation started to emerge.

In our lab, we aim at a full structural description of the highly dynamic process of translation. We use X-ray crystallography to obtain snapshots of different functional states of the ribosome and biochemistry to test the hypotheses concerning the mechanisms and dynamics of the ribosome and translation factors. These studies are designed not only to expand our fundamental knowledge of this molecular machine but also to aid in the development of new drugs that target ribosomes.

Translation termination on the 70S ribosome

Translation termination on the 70S ribosome.Release factor RF2 (yellow) is bound to the ribosome in response to a stop codon encoded in a messenger RNA (green) located on the small 30S ribosomal subunit (cyan and blue). RF2 is positioned to catalyze the hydrolysis of a peptidyl-tRNA (green and orange) on the large 50S subunit (grey, purple and magenta). An E-site-bound tRNA is in red.

This structural model was rendered in Pymol and is based on the X-ray structures of 70S translation termination complexes (Korostelev et al., 2008; Laurberg et al., 2008) and a 70S-tRNA complex (Jenner et al., 2010).

Rotation Projects

Rotation projects

We apply X-ray crystallography and biochemical methods to understand the mechanisms employed by the ribosome. Potential rotation projects are 1) to understand various aspects of translation via mutagenesis/biochemical assays, 2) to crystallize and work on determining the structures of translation factors and functional ribosome complexes, and 3) to improve computational methods used to determine crystal structures of macromolecules.

Feel free to contact the lab for a more detailed discussion.

One or more keywords matched the following items that are connected to Korostelev, Andrei
Item TypeName
Academic Article Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
Academic Article The ribosome in focus: new structures bring new insights.
Academic Article Interactions and dynamics of the Shine Dalgarno helix in the 70S ribosome.
Academic Article Structural basis for translation termination on the 70S ribosome.
Academic Article Structural dynamics of the ribosome.
Academic Article Crystal structure of a translation termination complex formed with release factor RF2.
Academic Article Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome.
Academic Article Crystal structure of the 70S ribosome bound with the Q253P mutant form of release factor RF2.
Academic Article Structure of the ribosome with elongation factor G trapped in the pretranslocation state.
Academic Article Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements.
Academic Article Analysis of structural dynamics in the ribosome by TLS crystallographic refinement.
Academic Article Multistart simulated annealing refinement of the crystal structure of the 70S ribosome.
Academic Article Structural aspects of translation termination on the ribosome.
Academic Article Crystal structures of 70S ribosomes bound to release factors RF1, RF2 and RF3.
Academic Article Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome.
Concept Ribosome Subunits
Concept Ribosome Subunits, Large, Bacterial
Concept Ribosome Subunits, Small
Concept Ribosome Subunits, Small, Eukaryotic
Concept Ribosome Subunits, Small, Bacterial
Concept Ribosome Subunits, Large
Concept Ribosomes
Academic Article Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center.
Academic Article Structures of yeast 80S ribosome-tRNA complexes in the rotated and nonrotated conformations.
Academic Article A deeper look into translation initiation.
Academic Article Initiation of translation in bacteria by a structured eukaryotic IRES RNA.
Academic Article Ribosome Structure Reveals Preservation of Active Sites in the Presence of a P-Site Wobble Mismatch.
Academic Article MMB-GUI: a fast morphing method demonstrates a possible ribosomal tRNA translocation trajectory.
Academic Article Structural Basis for Translation Termination on a Pseudouridylated Stop Codon.
Academic Article Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.
Academic Article Ribosome•RelA structures reveal the mechanism of stringent response activation.
Academic Article Mechanism of ribosome rescue by ArfA and RF2.
Academic Article Ensemble cryo-EM elucidates the mechanism of translation fidelity.
Academic Article Conformational Control of Translation Termination on the 70S Ribosome.
Academic Article Structural dynamics of protein S1 on the 70S ribosome visualized by ensemble cryo-EM.
Academic Article Mechanism of Inhibition of Translation Termination by Blasticidin S.
Academic Article Mechanism of premature translation termination on a sense codon.
Academic Article Structure of RNA polymerase bound to ribosomal 30S subunit.
Academic Article The Genetic Landscape of Diamond-Blackfan Anemia.
Academic Article Extensive ribosome and RF2 rearrangements during translation termination.
Academic Article mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding.
Academic Article Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.
Academic Article Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.
Academic Article Termi-Luc: a versatile assay to monitor full-protein release from ribosomes.
Academic Article ArfB can displace mRNA to rescue stalled ribosomes.
Academic Article Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.
Academic Article Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation.
Academic Article Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP.
Concept Internal Ribosome Entry Sites
Academic Article The Structural Dynamics of Translation.
Academic Article Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
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