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Rotation Projects

Rotation Projects

  1. Study the interaction of the ssDNA annealing protein Bet and its cognate exonuclease with dsDNA ends. Compare wild type Bet with one or mutant Bet proteins.  Use both genetic and biochemical studies to answer the question of how this annealase binds to ssDNA and promotes recombination.
  2. Develop recombineering technologies for M. tuberculosis involving the electroporation of oligos containing clinically relevant single nucleotide polymorphisms (SNPs).
  3. Generate a recombineering system for Pseudomonas aeruginosa via the electroporation of PCR substrates into electrocompetent cells containing PA phage Red-like recombination systems. 
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  • Polymorphism Single Nucleotide