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Singer, Joshua

One or more keywords matched the following properties of Singer, Joshua

Property	Value
overview	Academic Background 1966, S.B., S.M., E.E., Massachusetts Institute of Technology 1970, Ph.D., Harvard University Ion channel behavior and cellular physiological and biochemical processes The aim of our research is to understand the molecular mechanismsinvolved in membrane electrical events and the relationship of theseevents to biochemical and physiological processes. Studies are carriedout using mammalian and amphibian single smooth muscle cells and othercell types as well as various expression systems including Xenopusoocytes. We are using voltage-clamp techniques for measuring macroscopicmembrane currents, the patch-clamp technique for measuring currentspassing through single ion channels, Ca²⁺imaging techniques for examining the temporal and spatial distribution ofcellular Ca²⁺, and molecular biologicaltechniques for studying cloned channels, identifying new channels, anddetermining various functional domains of channel proteins. We have beenexamining the voltage, chemical, mechanical, and ionic control of membranechannels; the mechanism of action of neurotransmitters, peptides, drugsand other agents; and the role of these in cellular function. Specifically, we have recently been interested in the direct effects offatty acids, sphingosine, steroids, and other biologically active lipidson smooth muscle and neuron ion channel activity; the role ofmechanically-gated ion channels in the control of stretch-inducedcontraction of smooth muscle; various Ca²⁺permeable nonselective cation channels activated by caffeine, NMDA andATP; and the relationship between ion channel gating and changes in thelocal concentration of cellular Ca²⁺.

Property

Value

overview

Academic Background

1966, S.B., S.M., E.E., Massachusetts Institute of Technology
1970, Ph.D., Harvard University

Ion channel behavior and cellular physiological and biochemical processes

The aim of our research is to understand the molecular mechanismsinvolved in membrane electrical events and the relationship of theseevents to biochemical and physiological processes. Studies are carriedout using mammalian and amphibian single smooth muscle cells and othercell types as well as various expression systems including Xenopusoocytes. We are using voltage-clamp techniques for measuring macroscopicmembrane currents, the patch-clamp technique for measuring currentspassing through single ion channels, Ca²⁺imaging techniques for examining the temporal and spatial distribution ofcellular Ca²⁺, and molecular biologicaltechniques for studying cloned channels, identifying new channels, anddetermining various functional domains of channel proteins. We have beenexamining the voltage, chemical, mechanical, and ionic control of membranechannels; the mechanism of action of neurotransmitters, peptides, drugsand other agents; and the role of these in cellular function. Specifically, we have recently been interested in the direct effects offatty acids, sphingosine, steroids, and other biologically active lipidson smooth muscle and neuron ion channel activity; the role ofmechanically-gated ion channels in the control of stretch-inducedcontraction of smooth muscle; various Ca²⁺permeable nonselective cation channels activated by caffeine, NMDA andATP; and the relationship between ion channel gating and changes in thelocal concentration of cellular Ca²⁺.

One or more keywords matched the following items that are connected to Singer, Joshua

Item Type	Name
Academic Article	Imaging Ca(2+) entering the cytoplasm through a single opening of a plasma membrane cation channel.
Academic Article	P2X7 purinoceptor expression in Xenopus oocytes is not sufficient to produce a pore-forming P2Z-like phenotype.
Academic Article	Direct regulation of ion channels by fatty acids.
Academic Article	Multiple types of Ca2+ channels in visceral smooth muscle cells.
Academic Article	Both membrane stretch and fatty acids directly activate large conductance Ca(2+)-activated K+ channels in vascular smooth muscle cells.
Academic Article	Multiple pathways responsible for the stretch-induced increase in Ca2+ concentration in toad stomach smooth muscle cells.
Academic Article	Coupling of a P2Z-like purinoceptor to a fatty acid-activated K(+) channel in toad gastric smooth muscle cells.
Academic Article	Cholinergic agonists suppress a potassium current in freshly dissociated smooth muscle cells of the toad.
Academic Article	Large-conductance Ca2+-activated K+ channels in freshly dissociated smooth muscle cells.
Academic Article	Identification and characterization of major ionic currents in isolated smooth muscle cells using the voltage-clamp technique.
Academic Article	Large conductance ca-activated k channels in smooth muscle cell membrane: reduction in unitary currents due to internal na ions.
Academic Article	Neurotransmitter regulation of ionic channels in freshly dissociated smooth muscle cells.
Academic Article	Stretch-activated ion channels in smooth muscle: a mechanism for the initiation of stretch-induced contraction.
Academic Article	Hyperpolarization-activated cationic channels in smooth muscle cells are stretch sensitive.
Academic Article	Modulation of BK(Ca) channel activity by fatty acids: structural requirements and mechanism of action.
Academic Article	Site of action of fatty acids and other charged lipids on BKCa channels from arterial smooth muscle cells.
Academic Article	Stretch activation of a toad smooth muscle K+ channel may be mediated by fatty acids.
Academic Article	Membrane currents and cholinergic regulation of K+ current in esophageal smooth muscle cells.
Academic Article	Dual regulation of M current in gastric smooth muscle cells: beta-adrenergic-muscarinic antagonism.
Academic Article	Voltage, ligand, and mechanically gated channels in freshly dissociated single smooth muscle cells.
Academic Article	Characterization of calcium-activated potassium channels in single smooth muscle cells using the patch-clamp technique.
Academic Article	Acetylcholine increases voltage-activated Ca2+ current in freshly dissociated smooth muscle cells.
Academic Article	Regulation of one type of Ca2+ current in smooth muscle cells by diacylglycerol and acetylcholine.
Academic Article	Arachidonic acid and other fatty acids directly activate potassium channels in smooth muscle cells.
Academic Article	Ca++-activated K+ channels in vertebrate smooth muscle cells.
Academic Article	Stretch-inactivated cationic channels in single smooth muscle cells.
Academic Article	Aluminofluoride activates hyperpolarization- and stretch-activated cationic channels in single smooth muscle cells.
Academic Article	Direct effects of fatty acids and other charged lipids on ion channel activity in smooth muscle cells.
Academic Article	Caffeine activates a Ca(2+)-permeable, nonselective cation channel in smooth muscle cells.
Academic Article	Simultaneous measurement of Ca2+ release and influx into smooth muscle cells in response to caffeine. A novel approach for calculating the fraction of current carried by calcium.
Academic Article	Membrane stretch directly activates large conductance Ca(2+)-activated K+ channels in mesenteric artery smooth muscle cells.
Academic Article	Structural requirements for charged lipid molecules to directly increase or suppress K+ channel activity in smooth muscle cells. Effects of fatty acids, lysophosphatidate, acyl coenzyme A and sphingosine.
Concept	Ion Channels
Concept	Large-Conductance Calcium-Activated Potassium Channels
Concept	Calcium Channels
Concept	Potassium Channels, Calcium-Activated
Concept	Potassium Channels

Search Criteria

Hyperpolarization Activated Cyclic Nucleotide Gated Channels