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Academic Background

Education:

  • B.A., Queens College, 1983
  • M.D., Eastern Virginia Medical School, 1986

Post-Graduate Training:

  • Internal Medicine - Washington Hospital Center, 1986-89
  • Infectious Diseases - University of Massachusetts Medical Center, 1989-92
Photo: Sharone Green, MD

Research Interests

  • Immunopathogenesis of viral infections
  • Human T cell responses to flavivirus infections including dengue and West Nile virus
  • Human immune responses to novel viral vaccines

My laboratory studies T lymphocyte responses to viral infections and their role in protection as well as immunopathogenesis of disease. The study of T cell responses to natural viral infections can be utilized towards the development and testing of viral vaccines. Dengue virus, yellow fever virus, West Nile virus and Japanese encephalitis viruses are the current model systems under study.

Dengue viruses

Dengue viruses, of which there are four serotypes, are transmitted from human-to-human by mosquitoes in tropical and subtropical regions of the world. In some individuals, dengue virus infections manifest as dengue hemorrhagic fever (DHF), which is characterized by plasma leakage, and can lead to life-threatening shock. Our laboratory hypothesizes that DHF is an example of immunopathology caused by the ‘over’ responses of memory T cells. The role of other arms of the adaptive immune system in regulating disease severity, such as pre-existing antibodies that mediate neutralization, enhancement and antibody-dependent cell cytotoxicity (ADCC) are also under investigation. As part of an ongoing multi-center international NIH-funded Program Project, I spent over 4 years in Thailand trying to elucidate the role of immune activation in the development of plasma leakage in children with DHF. These field studies are currently ongoing and valuble samples from these children are utilized in our laboratory to address these immune mechanisms in disease pathogenesis.

West Nile virus

West Nile virus (WNV), another mosquito-borne virus which is rapidly emerging in the United States, often produces subclinical infection or an acute viral syndrome, West Nile fever. Ina minority of infected people the infectioncauses neurological illness -l WNV encephalitis. CD8 + T cells can be demonstrated in the brains of individuals who have succumbed to WNV encephalitis.T cells likely play a role in protecting the host from neuroinvasion and once neuroinvasion has occurred, T cells may also play a role in disease pathogenesis. Studies are underway for detailed analysis of memory T cell responses in humans infected by WNV, including CTL responses and interferon gamma production by WNV-specific T cells using IFN-gamma ELISPOT and intracellular cytokine staining assays. In addition, we have established a murine model of WNV encephalitis to study the role of flavivirus cross-reactive T cells in protection from WNV illness as well as their role in disease pathogenesis.

Heterologous immunity to flaviviruses

Flaviviruses co-circulate in many parts of the world. Due to relatively conserved genomic sequences, the effect of pre-existing immunity to one flavivirus on host immune responses to a secondary flavivirus can vary. In the case of dengue viruses, secondary infection may lead to an increase in disease severity. Some human epidemiologic and animal model studies suggest that other combinations of flaviviruses may lead to protection from disease. My laboratory is investigating the role of pre-existent flavivirus cross-reactive T cell and antibody responses on epitope hierarchy, disease outcome and viral burden in murine models, including human HLA transgenic mice.

Novel vaccines

Novel vaccines, such as DNA vaccines and chimeric virus vaccines, may induce antibody and/or T cell memory in immunized individuals. My laboratory is actively involved in evaluating T cell responses to such novel vaccines in the hope of developing much needed first and second generation vaccines for the US and the developing world.

Rotation Projects

Sharone Green, M.D.

Academic Role: Associate Professor

Faculty Appointment(s) In:
Medicine

Other Affiliation(s):
Center for Infectious Disease and Vaccine Research

Potential Rotation Projects

  • Flavivirus-specific T lymphocytes -characterization of T lymphocytes (human or murine) with novel specificities for epitopes ofdengue, yellow fever, Japanese encephalitis or West Nile viruses; functional characterization of T cell effector responses; quantitation of epitope-specific CD4 and/or CD8 T cells
One or more keywords matched the following items that are connected to Green, Sharone
Item TypeName
Academic Article Early clinical and laboratory indicators of acute dengue illness.
Academic Article Dengue in the early febrile phase: viremia and antibody responses.
Academic Article Early CD69 expression on peripheral blood lymphocytes from children with dengue hemorrhagic fever.
Academic Article Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity.
Academic Article Immunopathological mechanisms in dengue and dengue hemorrhagic fever.
Academic Article Natural history of plasma leakage in dengue hemorrhagic fever: a serial ultrasonographic study.
Academic Article Cytokine gene expression and protein production in peripheral blood mononuclear cells of children with acute dengue virus infections.
Academic Article Immunopathologic mechanisms of dengue hemorrhagic fever and dengue shock syndrome.
Academic Article A live, attenuated recombinant West Nile virus vaccine.
Academic Article Virus-induced decline in soluble vascular endothelial growth receptor 2 is associated with plasma leakage in dengue hemorrhagic Fever.
Academic Article Dengue hemorrhagic fever: the sensitivity and specificity of the world health organization definition for identification of severe cases of dengue in Thailand, 1994-2005.
Academic Article Serotype-specific differences in the risk of dengue hemorrhagic fever: an analysis of data collected in Bangkok, Thailand from 1994 to 2006.
Academic Article Persistence of virus-specific immune responses in the central nervous system of mice after West Nile virus infection.
Academic Article Host genetic risk factors for West Nile virus infection and disease progression.
Academic Article CD8+ T cells use TRAIL to restrict West Nile virus pathogenesis by controlling infection in neurons.
Concept Fever
Concept Yellow fever virus
Concept West Nile Fever
Academic Article Characteristics of mild dengue virus infection in Thai children.
Academic Article Preliminary evaluation of near infrared spectroscopy as a method to detect plasma leakage in children with dengue hemorrhagic fever.
Search Criteria
  • Fever