Loading...
Header Logo
Keywords
Last Name
Institution

Connection

Search Results to Jane B Lian PhD

This is a "connection" page, showing the details of why an item matched the keywords from your search.

                     
                     

One or more keywords matched the following properties of Lian, Jane

PropertyValue
overview

Academic Background

Associate Professor of Biological Chemistry, Harvard Medical School, Boston

Senior Research Associate, Department of Orthopaedic Surgery, Children's Hospital Medical Center, Boston

Post-doctoral training: National Institutes of Health; Harvard Medical School, Children's Hospital.

Ph.D., Boston University School of Medicine

Molecular Mechanisms Regulating Skeletal Development and Metastasis of Cancer Cells to Bone

Bone tissue functions as a mechanically responsive structural component of the body and as a major organ essential for maintaining calcium and phosphate homeostasis. The skeleton is the target of numerous human genetic disorders and recently mouse models have identified new regulatory pathways that affect the skeleton. As a normal process of aging and hormonal changes after the menopause, skeletal mass can decrease by as much as 30% leading to bone fracture and compromised quality of life in the elderly population. Historically the laboratory has addressed molecular mechanisms regulating formation and mineralization of bone by osteoblasts and turnover of bone tissue by osteoclasts, the bone resorbing cells. We are defining the key regulatory events for the progressive differentiation of osteoprogenitor stem cells to osteogenic cells by identifying transcription factor complexes that control expression of tissue-specific genes. Our studies are showing that early events of skeletal development are recapitulated in the adult skeleton for the normal maintenance of bone mass.

The skeleton is also a target of metastatic cancers and recent studies from our laboratory and others are demonstrating that signaling pathways which mediate responsiveness of the bone forming and bone resorbing cells of the skeleton for organogenesis are also pathways that are activated in cancer cells which metastasize to bone.

Areas of current investigation for graduate and MD/PhD students and postdoctoral fellows include:

1.   Combinatorial Control Mechanisms for Skeletal Development

This laboratory is defining a regulatory network of developmental factors with a focus on three major pathways critical for skeletal pattern formation, early embryonic bone development, and osteoblast differentiation:  the bone morphogenetic protein (BMP) family, the Wnt signaling pathway and Hox homeodomain factors.  The integration of these signaling pathways for tissue-specific gene expression is being characterized for two genes, the transcription factor Runx2/Cbfa1 established as essential for skeletal development and a bone-specific Runx2 target gene, osteocalcin, that represents one of the major non-collagenous bone matrix proteins (Choi et al., 2001; Lengner et al., 2002).  The Runx2/Cbfa transcription factor is essential for osteogenic differentiation and functions as a master regulatory gene through multiple properties (Fig. 1). Runx2 can change the phenotype of a cell, e.g., from a non-osseous adipocyte to an osteoblast, as well as control recruitment of stem cells into the chondrogenic and osteogenic phenotype (Lengner et al., 2005).  Characterizing the functional activities of this protein has provided new paradigms for understanding gene regulation.  First, Runx2 expression is regulated in stem cells by early development cues including Hox genes, BMP/TGFb, and Wnt proteins (Balint et al., 2003, Zaidi et al., 2002; Gaur et al., 2005, 2006; Bodine et al., 2004).  Secondly, this transcription factor is a scaffolding protein that is targeted to specific subnuclear domains for the assembly of multimeric complexes on target genes (reviewed in Lian et al., 2004).  Third, Runx2 recruits chromatin remodeling proteins and assembles complexes at Runx2 regulatory elements in genes to either activate or repress gene transcription (Javed et al., 1999; Young et al., 2005).  By mutational analyses of different protein interacting domains of Runx2, we are identifying the coregulatory proteins essential for osteoblast differentiation in vitro and for skeletal development in vivo (Zaidi et al., 2004; Afzal et al., 2005). Experimental approaches include cell culture models for chondrocyte and osteoblast differentiation using human embryonic and mesenchymal stem cells, gene regulation studies and characterization of mouse phenotypes. 

2.  Tissue Specific Gene Regulatory Mechanisms

The osteocalcin gene encodes a calcium binding ECM protein that is developmentally regulated during bone formation by a plethora of hormones, growth factors, and cell signaling proteins involved in calcium homeostasis and bone remodeling. The integration of independent signals must converge on the promoter to account for complex physiologic control of the gene during bone formation and turnover. Thus, the promoter of the osteocalcin gene provides a molecular blueprint for understanding developmental and hormonal regulation of gene expression required for bone formation (Hassan et al., 2004; Javed et al., 1999; Gutierrez et al., 2004). Identification of tissue-specific regulatory sequences and their cognate binding factors have allowed us to develop strategies for cell based therapy to target therapeutic genes specifically to bone. Examples of osteocalcin tissue-specific regulatory elements undergoing characterization include the steroid response elements, runt homology (Runx/Cbfa), homeodomain (HD), Hox, C/EBP, ATF and AP-1 protein binding sites.  The HD proteins (Msx, Dlx) and Hoxa10 regulate osteocalcin at different stages of cell differentiation (Fig. 2). Transcriptional control of the gene is evaluated at multiple levels including chromatin modifications and recruitment of transcription factors and their coregulatory proteins by chromatin immunoprecipitation studies.  Enforced expression and siRNA knockdown studies of regulatory factors strategies are used to establish their function in vitro and in vivo using transgenic mouse models. Such analyses have revealed new insights into understanding the complex interplay of physiologic mediators of tissue differentiation. This program of research provides a student with fundamental techniques requisite for characterizing gene regulation and expression in a biological context.

3.  Cancer Cell Biology in the Bone Microenvironment

The end stage of breast and prostate cancer is metastasis to bone, with very poor prognosis with nearly 70% mortality within a year.  Cancer cells cause destruction of the bone, resulting in fractures and severe pain.  Understanding the mechanisms which induce metastasis of the primary cancer cell to the bone environment needs to be addressed.  We have identified high expression levels of the Runx2 transcription factor in metastatic breast and prostate cancer cell lines.  Runx target genes in the cancer cell include the entire class of matrix metalloproteinases characterized for their role in tissue invasion, the vascular endothelial growth factor, a potent angiogenic factor involved as a primary event in tumor growth and several cell growth and osteoblastic genes expressed in the bone environment that allow for tumor growth (Fig. 3) (Pratap et al., 2005).  The cancer cell responds to TGFb and BMP growth factors in the bone extracellular matrix and stimulates bone resorbing cells.  In recent studies, we have shown metastatic cancer cell lines in which Runx2 activity has been blocked through genetic mutations, that the osteolytic disease of breast cancer cells can be prevented in the mouse (Barnes et al., 2004; Javed et al., 2005). The presence of mutant Runx2 protein in metastatic cells inhibits cell invasion (in vitro assays) and genes associated with tumor growth (Fig. 3).  We are now turning our attention to mechanisms responsible for activation of Runx2 in the primary tumor that would lead to the metastatic event in vivo.  Experimental approaches include generation of human cancer cell lines with mutants of Runx2, assessing tumor growth by in vivo imaging of tumors in breast, prostate and bone tissues, and examining gene expression profiles of the tumors are assayed.

4.  Gene Therapy and Tissue Engineering for Skeletal Diseases

Genetic disorders of the skeleton require expression of the normal proteins, specifically in bone cells.   Here the osteocalcin gene promoter provides the appropriate method for targeting expressed genes to mature osteoblasts.  Using OC-EGFP mice, we are characterizing a population of mesenchymal stem cells from these mice that differentiate into bone cells expressing GFP in donor mice.  Numerous donor mice carrying mutated genes that mimic human genetic disorders are available for addressing the critical number of mesenchymal stem cells differentiated to osteoblasts that can provide a sufficient level of normal protein to correct the skeletal disorder.  A second example where tissue engineering is being developed relates to the problem of non-union fractures where scar tissue rather than new bone formation occurs.  By using autologous cells expressing osteogenic factors, bone formation can be induced.  Lastly, we are culturing human embryonic stem cells and promoting their differentiation to chondrogenic and osteogenic lineages and testing these in mouse models.  These powerful properties are being exploited for treatment of skeletal diseases that require rebuilding of skeletal tissue by promoting lineage allocation of stem cells or marrow progenitor cells. 

Figures

 Figure 1

 

  Lian Figure 1

 Figure 2

 

  Lian Figure 2
  

 Figure 3

 

  Lian Figure 3
  

 


One or more keywords matched the following items that are connected to Lian, Jane

Item TypeName
Academic Article CCAAT/enhancer-binding proteins (C/EBP) beta and delta activate osteocalcin gene transcription and synergize with Runx2 at the C/EBP element to regulate bone-specific expression.
Academic Article Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites.
Academic Article Identification of novel protein/DNA interactions within the promoter of the bone-related transcription factor Runx2/Cbfa1.
Academic Article Transcription factors RUNX1/AML1 and RUNX2/Cbfa1 dynamically associate with stationary subnuclear domains.
Academic Article Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression.
Academic Article Phenotype discovery by gene expression profiling: mapping of biological processes linked to BMP-2-mediated osteoblast differentiation.
Academic Article Intranuclear organization of RUNX transcriptional regulatory machinery in biological control of skeletogenesis and cancer.
Academic Article Nuclear microenvironments support physiological control of gene expression.
Academic Article Cell growth regulatory role of Runx2 during proliferative expansion of preosteoblasts.
Academic Article Functional architecture of the nucleus: organizing the regulatory machinery for gene expression, replication and repair.
Academic Article Differential gene expression analysis using paraffin-embedded tissues after laser microdissection.
Academic Article Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription.
Academic Article Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression.
Academic Article Primary mouse embryonic fibroblasts: a model of mesenchymal cartilage formation.
Academic Article In situ immunofluorescence analysis: immunofluorescence microscopy.
Academic Article In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling.
Academic Article Chromatin immunoprecipitation.
Academic Article Intranuclear trafficking: organization and assembly of regulatory machinery for combinatorial biological control.
Academic Article The vitamin D response element in the distal osteocalcin promoter contributes to chromatin organization of the proximal regulatory domain.
Academic Article Overlapping expression of Runx1(Cbfa2) and Runx2(Cbfa1) transcription factors supports cooperative induction of skeletal development.
Academic Article Smad function and intranuclear targeting share a Runx2 motif required for osteogenic lineage induction and BMP2 responsive transcription.
Academic Article Impaired intranuclear trafficking of Runx2 (AML3/CBFA1) transcription factors in breast cancer cells inhibits osteolysis in vivo.
Academic Article Nkx3.2-mediated repression of Runx2 promotes chondrogenic differentiation.
Academic Article Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype.
Academic Article Brg1, the ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for myeloid differentiation to granulocytes.
Academic Article Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression.
Academic Article Microtubule-dependent nuclear-cytoplasmic shuttling of Runx2.
Academic Article Combinatorial organization of the transcriptional regulatory machinery in biological control and cancer.
Academic Article Subnuclear targeting of Runx1 is required for synergistic activation of the myeloid specific M-CSF receptor promoter by PU.1.
Academic Article Lymphocyte enhancer-binding factor 1 (Lef1) inhibits terminal differentiation of osteoblasts.
Academic Article Secreted frizzled related protein 1 regulates Wnt signaling for BMP2 induced chondrocyte differentiation.
Academic Article The classic receptor for 1alpha,25-dihydroxy vitamin D3 is required for non-genomic actions of 1alpha,25-dihydroxy vitamin D3 in osteosarcoma cells.
Academic Article Alterations in intranuclear localization of Runx2 affect biological activity.
Academic Article An architectural perspective of cell-cycle control at the G1/S phase cell-cycle transition.
Academic Article Networks and hubs for the transcriptional control of osteoblastogenesis.
Academic Article Mitotic control of RUNX2 phosphorylation by both CDK1/cyclin B kinase and PP1/PP2A phosphatase in osteoblastic cells.
Academic Article Temporal evolution of gene expression in rat carotid artery following balloon angioplasty.
Academic Article Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2.
Academic Article Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter.
Academic Article HOXA10 controls osteoblastogenesis by directly activating bone regulatory and phenotypic genes.
Academic Article Mitotic retention of gene expression patterns by the cell fate-determining transcription factor Runx2.
Academic Article Organization of transcriptional regulatory machinery in nuclear microenvironments: implications for biological control and cancer.
Academic Article Cooperation between p27 and p107 during endochondral ossification suggests a genetic pathway controlled by p27 and p130.
Academic Article Cell cycle dependent phosphorylation and subnuclear organization of the histone gene regulator p220(NPAT) in human embryonic stem cells.
Academic Article Nuclear microenvironments in biological control and cancer.
Academic Article Nucleosome organization and targeting of SWI/SNF chromatin-remodeling complexes: contributions of the DNA sequence.
Academic Article Ajulemic acid, a nonpsychoactive cannabinoid acid, suppresses osteoclastogenesis in mononuclear precursor cells and induces apoptosis in mature osteoclast-like cells.
Academic Article Cell cycle related modulations in Runx2 protein levels are independent of lymphocyte enhancer-binding factor 1 (Lef1) in proliferating osteoblasts.
Academic Article Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential.
Academic Article Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal.
Academic Article Vitamin D control of gene expression: temporal and spatial parameters for organization of the regulatory machinery.
Academic Article Phenotypic transcription factors epigenetically mediate cell growth control.
Academic Article Chromatin immunoprecipitation assays: application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells.
Academic Article Molecular consequences of a frameshifted DLX3 mutant leading to Tricho-Dento-Osseous syndrome.
Academic Article Expression of secreted frizzled related protein 1, a Wnt antagonist, in brain, kidney, and skeleton is dispensable for normal embryonic development.
Academic Article Elastomeric high-mineral content hydrogel-hydroxyapatite composites for orthopedic applications.
Academic Article Runx2 regulates G protein-coupled signaling pathways to control growth of osteoblast progenitors.
Academic Article Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain.
Academic Article Intricate gene regulatory networks of helix-loop-helix (HLH) proteins support regulation of bone-tissue related genes during osteoblast differentiation.
Academic Article Molecular switches involving homeodomain proteins, HOXA10 and RUNX2 regulate osteoblastogenesis.
Academic Article New insights in bone biology: unmasking skeletal effects of the extracellular calcium-sensing receptor.
Academic Article A microRNA signature for a BMP2-induced osteoblast lineage commitment program.
Academic Article Runx2 transcriptional activation of Indian Hedgehog and a downstream bone metastatic pathway in breast cancer cells.
Academic Article The histone gene cell cycle regulator HiNF-P is a unique zinc finger transcription factor with a novel conserved auxiliary DNA-binding motif.
Academic Article Altered chromatin modifications in AML1/RUNX1 breakpoint regions involved in (8;21) translocation.
Academic Article Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes.
Academic Article Co-stimulation of the bone-related Runx2 P1 promoter in mesenchymal cells by SP1 and ETS transcription factors at polymorphic purine-rich DNA sequences (Y-repeats).
Academic Article A Runx2 threshold for the cleidocranial dysplasia phenotype.
Academic Article The osteogenic transcription factor Runx2 regulates components of the fibroblast growth factor/proteoglycan signaling axis in osteoblasts.
Academic Article The subnuclear organization of histone gene regulatory proteins and 3' end processing factors of normal somatic and embryonic stem cells is compromised in selected human cancer cell types.
Academic Article Secreted frizzled related protein 1 is a target to improve fracture healing.
Academic Article Biological functions of miR-29b contribute to positive regulation of osteoblast differentiation.
Academic Article The osteogenic transcription factor runx2 controls genes involved in sterol/steroid metabolism, including CYP11A1 in osteoblasts.
Academic Article Survival responses of human embryonic stem cells to DNA damage.
Academic Article Prostate cancer regulatory networks.
Academic Article The Notch-responsive transcription factor Hes-1 attenuates osteocalcin promoter activity in osteoblastic cells.
Academic Article Ectopic runx2 expression in mammary epithelial cells disrupts formation of normal acini structure: implications for breast cancer progression.
Academic Article Human embryonic stem cells are pre-mitotically committed to self-renewal and acquire a lengthened G1 phase upon lineage programming.
Academic Article Cyclin D2 and the CDK substrate p220(NPAT) are required for self-renewal of human embryonic stem cells.
Academic Article Transcription factor-mediated epigenetic regulation of cell growth and phenotype for biological control and cancer.
Academic Article Expression of the osteoblast differentiation factor RUNX2 (Cbfa1/AML3/Pebp2alpha A) is inhibited by tumor necrosis factor-alpha.
Academic Article Definitive hematopoiesis requires Runx1 C-terminal-mediated subnuclear targeting and transactivation.
Academic Article The cancer-related Runx2 protein enhances cell growth and responses to androgen and TGFbeta in prostate cancer cells.
Academic Article Transcriptional corepressor TLE1 functions with Runx2 in epigenetic repression of ribosomal RNA genes.
Academic Article A central dinucleotide within vitamin D response elements modulates DNA binding and transactivation by the vitamin D receptor in cellular response to natural and synthetic ligands.
Academic Article Genetic ablation of the CDP/Cux protein C terminus results in hair cycle defects and reduced male fertility.
Academic Article The gene for aromatase, a rate-limiting enzyme for local estrogen biosynthesis, is a downstream target gene of Runx2 in skeletal tissues.
Academic Article The human SWI/SNF complex associates with RUNX1 to control transcription of hematopoietic target genes.
Academic Article Frequent attenuation of the WWOX tumor suppressor in osteosarcoma is associated with increased tumorigenicity and aberrant RUNX2 expression.
Academic Article Reduced CpG methylation is associated with transcriptional activation of the bone-specific rat osteocalcin gene in osteoblasts.
Academic Article Histone acetylation in vivo at the osteocalcin locus is functionally linked to vitamin D-dependent, bone tissue-specific transcription.
Academic Article 1,25-(OH)2-vitamin D3 suppresses the bone-related Runx2/Cbfa1 gene promoter.
Academic Article Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets.
Academic Article Preclinical characterization of mitochondria-targeted small molecule hsp90 inhibitors, gamitrinibs, in advanced prostate cancer.
Academic Article Live cell imaging of the cancer-related transcription factor RUNX2 during mitotic progression.
Academic Article A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program.
Academic Article Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility.
Academic Article Architectural genetic and epigenetic control of regulatory networks: compartmentalizing machinery for transcription and chromatin remodeling in nuclear microenvironments.
Academic Article Effects of miR-335-5p in modulating osteogenic differentiation by specifically downregulating Wnt antagonist DKK1.
Academic Article TIEG1/KLF10 modulates Runx2 expression and activity in osteoblasts.
Academic Article Epigenetic regulation of early osteogenesis and mineralized tissue formation by a HOXA10-PBX1-associated complex.
Academic Article Runx2 protein expression utilizes the Runx2 P1 promoter to establish osteoprogenitor cell number for normal bone formation.
Academic Article Forum on aging and skeletal health: summary of the proceedings of an ASBMR workshop.
Academic Article MicroRNA control of bone formation and homeostasis.
Academic Article A RUNX2-HDAC1 co-repressor complex regulates rRNA gene expression by modulating UBF acetylation.
Academic Article Control of mesenchymal lineage progression by microRNAs targeting skeletal gene regulators Trps1 and Runx2.
Academic Article The abbreviated pluripotent cell cycle.
Academic Article Genomic occupancy of HLH, AP1 and Runx2 motifs within a nuclease sensitive site of the Runx2 gene.
Academic Article miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells.
Academic Article Runx2 (Cbfa1, AML-3) interacts with histone deacetylase 6 and represses the p21(CIP1/WAF1) promoter.
Academic Article Multiple subnuclear targeting signals of the leukemia-related AML1/ETO and ETO repressor proteins.
Academic Article The core-binding factor beta subunit is required for bone formation and hematopoietic maturation.
Academic Article Cbfbeta interacts with Runx2 and has a critical role in bone development.
Academic Article Transcriptional induction of the osteocalcin gene during osteoblast differentiation involves acetylation of histones h3 and h4.
Academic Article Pin1-mediated Runx2 modification is critical for skeletal development.
Academic Article Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the vitamin D3 receptor but not p300 intrinsic histone acetyltransferase activity.
Academic Article Osteoblast-related transcription factors Runx2 (Cbfa1/AML3) and MSX2 mediate the expression of bone sialoprotein in human metastatic breast cancer cells.
Academic Article Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo.
Academic Article Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing.
Academic Article Ajulemic acid, a nonpsychoactive cannabinoid acid, induces apoptosis in human T lymphocytes.
Academic Article Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells.
Academic Article Nuclear microenvironments support assembly and organization of the transcriptional regulatory machinery for cell proliferation and differentiation.
Academic Article Regulatory controls for osteoblast growth and differentiation: role of Runx/Cbfa/AML factors.
Academic Article The Runx2 transcription factor plays a key role in the 1alpha,25-dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells.
Academic Article Fidelity of Runx2 activity in breast cancer cells is required for the generation of metastases-associated osteolytic disease.
Academic Article Protein-deoxyribonucleic acid interactions linked to gene expression: DNase I digestion.
Academic Article Quantitative signature for architectural organization of regulatory factors using intranuclear informatics.
Academic Article Bone-specific transcription factor Runx2 interacts with the 1alpha,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells.
Academic Article Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene.
Academic Article SWI/SNF chromatin remodeling complex is obligatory for BMP2-induced, Runx2-dependent skeletal gene expression that controls osteoblast differentiation.
Academic Article The tyrosine phosphatase, OST-PTP, is expressed in mesenchymal progenitor cells early during skeletogenesis in the mouse.
Academic Article The dynamic organization of gene-regulatory machinery in nuclear microenvironments.
Academic Article The bone-specific expression of Runx2 oscillates during the cell cycle to support a G1-related antiproliferative function in osteoblasts.
Academic Article Organization of transcriptional regulatory machinery in osteoclast nuclei: compartmentalization of Runx1.
Academic Article The Wnt antagonist secreted frizzled-related protein-1 controls osteoblast and osteocyte apoptosis.
Academic Article The Runx2 osteogenic transcription factor regulates matrix metalloproteinase 9 in bone metastatic cancer cells and controls cell invasion.
Academic Article Four novel RUNX2 mutations including a splice donor site result in the cleidocranial dysplasia phenotype.
Academic Article The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells.
Academic Article Osteoblast differentiation and skeletal development are regulated by Mdm2-p53 signaling.
Academic Article Reconstitution of Runx2/Cbfa1-null cells identifies a requirement for BMP2 signaling through a Runx2 functional domain during osteoblast differentiation.
Academic Article Self-renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase.
Academic Article BMP2 commitment to the osteogenic lineage involves activation of Runx2 by DLX3 and a homeodomain transcriptional network.
Academic Article Establishment of histone gene regulation and cell cycle checkpoint control in human embryonic stem cells.
Academic Article Crystallization and preliminary X-ray analysis of a domain in the Runx2 transcription factor that interacts with the 1alpha,25 dihydroxy vitamin D3 receptor.
Academic Article An architectural perspective of vitamin D responsiveness.
Academic Article 1alpha,25-dihydroxy vitamin D3-enhanced expression of the osteocalcin gene involves increased promoter occupancy of basal transcription regulators and gradual recruitment of the 1alpha,25-dihydroxy vitamin D3 receptor-SRC-1 coactivator complex.
Academic Article Pharmacologic targeting of a stem/progenitor population in vivo is associated with enhanced bone regeneration in mice.
Academic Article In situ nuclear organization of regulatory machinery.
Academic Article The WWOX tumor suppressor is essential for postnatal survival and normal bone metabolism.
Academic Article Genetic and epigenetic regulation in nuclear microenvironments for biological control in cancer.
Academic Article Specific residues of RUNX2 are obligatory for formation of BMP2-induced RUNX2-SMAD complex to promote osteoblast differentiation.
Academic Article Staged assembly of histone gene expression machinery at subnuclear foci in the abbreviated cell cycle of human embryonic stem cells.
Academic Article The leukemogenic t(8;21) fusion protein AML1-ETO controls rRNA genes and associates with nucleolar-organizing regions at mitotic chromosomes.
Academic Article Transcription-factor-mediated epigenetic control of cell fate and lineage commitment.
Academic Article Organization, integration, and assembly of genetic and epigenetic regulatory machinery in nuclear microenvironments: implications for biological control in cancer.
Academic Article Sustained and localized in vitro release of BMP-2/7, RANKL, and tetracycline from FlexBone, an elastomeric osteoconductive bone substitute.
Academic Article Enhanced fracture repair by leukotriene antagonism is characterized by increased chondrocyte proliferation and early bone formation: a novel role of the cysteinyl LT-1 receptor.
Academic Article SWI/SNF-independent nuclease hypersensitivity and an increased level of histone acetylation at the P1 promoter accompany active transcription of the bone master gene Runx2.
Academic Article The histone gene activator HINFP is a nonredundant cyclin E/CDK2 effector during early embryonic cell cycles.
Academic Article Impaired cell cycle regulation of the osteoblast-related heterodimeric transcription factor Runx2-Cbfbeta in osteosarcoma cells.
Academic Article Altered Runx1 subnuclear targeting enhances myeloid cell proliferation and blocks differentiation by activating a miR-24/MKP-7/MAPK network.
Academic Article 1alpha,25-dihydroxy vitamin D(3) induces nuclear matrix association of the 1alpha,25-dihydroxy vitamin D(3) receptor in osteoblasts independently of its ability to bind DNA.
Academic Article Dicer inactivation in osteoprogenitor cells compromises fetal survival and bone formation, while excision in differentiated osteoblasts increases bone mass in the adult mouse.
Academic Article Recruitment and subnuclear distribution of the regulatory machinery during 1alpha,25-dihydroxy vitamin D3-mediated transcriptional upregulation in osteoblasts.
Academic Article The cleidocranial dysplasia-related R131G mutation in the Runt-related transcription factor RUNX2 disrupts binding to DNA but not CBF-beta.
Academic Article Pbx1 represses osteoblastogenesis by blocking Hoxa10-mediated recruitment of chromatin remodeling factors.
Academic Article Metastatic bone disease: role of transcription factors and future targets.
Academic Article Mitotic bookmarking of genes: a novel dimension to epigenetic control.
Academic Article Subnuclear localization and intranuclear trafficking of transcription factors.
Academic Article Architectural epigenetics: mitotic retention of mammalian transcriptional regulatory information.
Academic Article A proteasome inhibitor, bortezomib, inhibits breast cancer growth and reduces osteolysis by downregulating metastatic genes.
Academic Article Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells.
Academic Article The histone deacetylase inhibitor, vorinostat, reduces tumor growth at the metastatic bone site and associated osteolysis, but promotes normal bone loss.
Academic Article An architectural genetic and epigenetic perspective.
Academic Article Suberoylanilide hydroxamic acid (SAHA; vorinostat) causes bone loss by inhibiting immature osteoblasts.
Academic Article C/EBPß binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells.
Academic Article Functional coupling of transcription factor HiNF-P and histone H4 gene expression during pre- and post-natal mouse development.
Academic Article A program of microRNAs controls osteogenic lineage progression by targeting transcription factor Runx2.
Academic Article Genomic promoter occupancy of runt-related transcription factor RUNX2 in Osteosarcoma cells identifies genes involved in cell adhesion and motility.
Academic Article miRNA signatures associate with pathogenesis and progression of osteosarcoma.
Academic Article Neural crest deletion of Dlx3 leads to major dentin defects through down-regulation of Dspp.
Academic Article The architectural organization of human stem cell cycle regulatory machinery.
Academic Article Runx2 mediates epigenetic silencing of the bone morphogenetic protein-3B (BMP-3B/GDF10) in lung cancer cells.
Academic Article Epigenetic mechanisms in leukemia.
Academic Article In vivo impact of Dlx3 conditional inactivation in neural crest-derived craniofacial bones.
Academic Article Conditional inactivation of the mouse Wwox tumor suppressor gene recapitulates the null phenotype.
Academic Article Runx2 protein represses Axin2 expression in osteoblasts and is required for craniosynostosis in Axin2-deficient mice.
Academic Article Oncogenic cooperation between PI3K/Akt signaling and transcription factor Runx2 promotes the invasive properties of metastatic breast cancer cells.
Academic Article Targeting deregulated epigenetic control in cancer.
Academic Article MicroRNA-34c inversely couples the biological functions of the runt-related transcription factor RUNX2 and the tumor suppressor p53 in osteosarcoma.
Concept Epithelial Cells
Concept Cell Lineage
Concept Cell Physiological Phenomena
Concept Hela Cells
Concept Cell Nucleus
Concept Cell Division
Concept COS Cells
Concept Cell Communication
Concept Cell Survival
Concept Cell Adhesion
Concept NIH 3T3 Cells
Concept Jurkat Cells
Concept Cell Cycle Proteins
Concept Mesenchymal Stem Cell Transplantation
Concept CHO Cells
Concept 3T3 Cells
Concept Tumor Cells, Cultured
Concept Multipotent Stem Cells
Concept Cell Differentiation
Concept Cell Line, Transformed
Concept Bone Marrow Cells
Concept Cell Fractionation
Concept Cell Polarity
Concept Cell Movement
Concept Cells, Cultured
Concept Stem Cells
Concept Rod Cell Outer Segment
Concept Cell Proliferation
Concept Hematopoietic Stem Cells
Concept Cell Nucleolus
Concept Active Transport, Cell Nucleus
Concept Cell Cycle
Concept Cell Aging
Concept HL-60 Cells
Concept Cell Culture Techniques
Concept Eukaryotic Cells
Concept Myeloid Progenitor Cells
Concept Cell Transformation, Neoplastic
Concept Induced Pluripotent Stem Cells
Concept Stromal Cells
Concept Embryonic Stem Cells
Concept Cell Membrane Permeability
Concept Cell Growth Processes
Concept Cell Line
Concept Cell Compartmentation
Concept Pluripotent Stem Cells
Concept Cell Shape
Concept Myeloid Cells
Concept Cell Line, Tumor
Concept Cell Count
Academic Article Genomic occupancy of Runx2 with global expression profiling identifies a novel dimension to control of osteoblastogenesis.
Academic Article A germline point mutation in Runx1 uncouples its role in definitive hematopoiesis from differentiation.
Academic Article The dynamic architectural and epigenetic nuclear landscape: developing the genomic almanac of biology and disease.
Academic Article Standardizing analysis of circulating microRNA: clinical and biological relevance.
Academic Article Integrin avß6 promotes an osteolytic program in cancer cells by upregulating MMP2.
Academic Article Bookmarking target genes in mitosis: a shared epigenetic trait of phenotypic transcription factors and oncogenes?
Academic Article Epigenetic landscape during osteoblastogenesis defines a differentiation-dependent Runx2 promoter region.
Academic Article A functional N-terminal domain in C/EBPß-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.
Academic Article Core binding factor ß (CBFß) is retained in the midbody during cytokinesis.
Academic Article hsa-mir-30c promotes the invasive phenotype of metastatic breast cancer cells by targeting NOV/CCN3.
Academic Article The bone-specific Runx2-P1 promoter displays conserved three-dimensional chromatin structure with the syntenic Supt3h promoter.
Academic Article Disruption of crosstalk between mesenchymal stromal and tumor cells in bone marrow as a therapeutic target to prevent metastatic bone disease.
Academic Article Subnuclear domain proteins in cancer cells support the functions of RUNX2 in the DNA damage response.
Academic Article Fidelity of histone gene regulation is obligatory for genome replication and stability.
Academic Article microRNA-mediated survivin control of pluripotency.
Academic Article Runx2-Smad signaling impacts the progression of tumor-induced bone disease.
Academic Article CBFß and the leukemogenic fusion protein CBFß-SMMHC associate with mitotic chromosomes to epigenetically regulate ribosomal genes.
Academic Article Could lncRNAs be the missing links in control of mesenchymal stem cell differentiation?
Academic Article Core binding factor ß of osteoblasts maintains cortical bone mass via stabilization of Runx2 in mice.
Academic Article Targeting of Runx2 by miR-135 and miR-203 Impairs Progression of Breast Cancer and Metastatic Bone Disease.
Academic Article Genome-wide co-occupancy of AML1-ETO and N-CoR defines the t(8;21) AML signature in leukemic cells.
Academic Article Runx1 is associated with breast cancer progression in MMTV-PyMT transgenic mice and its depletion in vitro inhibits migration and invasion.
Academic Article Expression of the IL-11 Gene in Metastatic Cells Is Supported by Runx2-Smad and Runx2-cJun Complexes Induced by TGFß1.
Academic Article p53 checkpoint ablation exacerbates the phenotype of Hinfp dependent histone H4 deficiency.
Academic Article The SWI/SNF ATPases Are Required for Triple Negative Breast Cancer Cell Proliferation.
Academic Article Runx1 Activities in Superficial Zone Chondrocytes, Osteoarthritic Chondrocyte Clones and Response to Mechanical Loading.
Academic Article C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization.
Academic Article Chromatin interaction analysis reveals changes in small chromosome and telomere clustering between epithelial and breast cancer cells.
Academic Article Non-coding RNAs: Epigenetic regulators of bone development and homeostasis.
Academic Article Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B.
Academic Article Chromosomes at Work: Organization of Chromosome Territories in the Interphase Nucleus.
Academic Article Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation.
Academic Article Epigenetic Modulation in Periodontitis: Interaction of Adiponectin and JMJD3-IRF4 Axis in Macrophages.
Academic Article Expression of Ribosomal RNA and Protein Genes in Human Embryonic Stem Cells Is Associated With the Activating H3K4me3 Histone Mark.
Academic Article Lineage-Specific Early Differentiation of Human Embryonic Stem Cells Requires a G2 Cell Cycle Pause.
Academic Article Oncofetal Epigenetic Bivalency in Breast Cancer Cells: H3K4 and H3K27 Tri-Methylation as a Biomarker for Phenotypic Plasticity.
Academic Article Thyroid Hormone Receptor-ß (TRß) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.
Academic Article MicroRNA-378-mediated suppression of Runx1 alleviates the aggressive phenotype of triple-negative MDA-MB-231 human breast cancer cells.
Academic Article SMARCA4 regulates gene expression and higher-order chromatin structure in proliferating mammary epithelial cells.
Academic Article Dissection of Individual Prostate Lobes in Mouse Models of Prostate Cancer to Obtain High Quality RNA.
Academic Article Runx2/DICER/miRNA Pathway in Regulating Osteogenesis.
Academic Article Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling.
Academic Article Antagonizing miR-218-5p attenuates Wnt signaling and reduces metastatic bone disease of triple negative breast cancer cells.
Academic Article RUNX1 contributes to higher-order chromatin organization and gene regulation in breast cancer cells.
Academic Article The BRG1 chromatin remodeling enzyme links cancer cell metabolism and proliferation.
Academic Article Identifying Nuclear Matrix-Attached DNA Across the Genome.
Academic Article Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells.
Academic Article Chromatin Dynamics Regulate Mesenchymal Stem Cell Lineage Specification and Differentiation to Osteogenesis.
Academic Article Unique Regulatory Mechanisms for the Human Embryonic Stem Cell Cycle.
Academic Article Precocious Phenotypic Transcription-Factor Expression During Early Development.
Academic Article A Perspective on Malignancy in the Marrow.
Academic Article Mitotic Gene Bookmarking: An Epigenetic Mechanism for Coordination of Lineage Commitment, Cell Identity and Cell Growth.
Academic Article Runx1 stabilizes the mammary epithelial cell phenotype and prevents epithelial to mesenchymal transition.
Academic Article Selective expression of long non-coding RNAs in a breast cancer cell progression model.
Academic Article Intranuclear and higher-order chromatin organization of the major histone gene cluster in breast cancer.
Academic Article An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells.
Academic Article Cover Image, Volume 232, Number 12, December 2017.
Academic Article Bivalent Epigenetic Control of Oncofetal Gene Expression in Cancer.
Academic Article Genome-wide DNase hypersensitivity, and occupancy of RUNX2 and CTCF reveal a highly dynamic gene regulome during MC3T3 pre-osteoblast differentiation.
Academic Article Suppression of Breast Cancer Stem Cells and Tumor Growth by the RUNX1 Transcription Factor.
Academic Article Linkages of nuclear architecture to biological and pathological control of gene expression.
Academic Article Loss of RUNX1 is associated with aggressive lung adenocarcinomas.
Academic Article Mll-COMPASS complexes mediate H3K4me3 enrichment and transcription of the osteoblast master gene Runx2/p57 in osteoblasts.
Academic Article Higher order genomic organization and regulatory compartmentalization for cell cycle control at the G1/S-phase transition.
Academic Article High throughput analysis of differential gene expression.
Academic Article Osteocalcin gene promoter: Unlocking the secrets for regulation of osteoblast growth and differentiation.
Academic Article Mitotically-Associated lncRNA (MANCR) Affects Genomic Stability and Cell Division in Aggressive Breast Cancer.
Academic Article Nanoparticle-based targeted cancer strategies for non-invasive prostate cancer intervention.
Academic Article Epithelial-to-mesenchymal transition and cancer stem cells contribute to breast cancer heterogeneity.
Academic Article Ethyl acetate and n-butanol fraction of Cissus quadrangularis promotes the mineralization potential of murine pre-osteoblast cell line MC3T3-E1 (sub-clone 4).
Academic Article tsRNA signatures in cancer.
Academic Article RUNX1-dependent mechanisms in biological control and dysregulation in cancer.
Academic Article Osteogenic potential of hexane and dichloromethane fraction of Cissus quadrangularis on murine preosteoblast cell line MC3T3-E1 (subclone 4).
Academic Article Switches in histone modifications epigenetically control vitamin D3-dependent transcriptional upregulation of the CYP24A1 gene in osteoblastic cells.
Academic Article Ezh2-dependent H3K27me3 modification dynamically regulates vitamin D3-dependent epigenetic control of CYP24A1 gene expression in osteoblastic cells.
Academic Article Identification of tRNA-derived small RNA (tsRNA) responsive to the tumor suppressor, RUNX1, in breast cancer.
Academic Article RUNX1 and RUNX2 transcription factors function in opposing roles to regulate breast cancer stem cells.

Search Criteria
  • Somatostatin Secreting Cells