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One or more keywords matched the following properties of Florman, Harvey

Academic Background

1972 B.A., Rutgers College
1982 Ph.D., University of Pennsylvania

Mechanisms of Fertilization

Fertilization is the process by which sperm and egg fuse and the program of development is initiated. This is controlled in mammals at two steps: 1) sperm first undergo “capacitation”, a physiological reprogramming that occurs within the female reproductive tract and that is essential for the expression of fertility; and 2) capacitated sperm interact with the egg. Our work has focused on the molecular and cellular mechanisms that control these two events. To this end, we apply a range of biophysical (single cell fluorescence imaging, patch clamp electrophysiology), biochemical and molecular techniques.

Studies of capacitation address the control of sperm signal transduction pathways that lead to fertility. Ion fluxes are important regulators of this process and we have identified key roles of sperm membrane potential and pH. In addition, we recently recognized a role of polycystic kidney disease proteins in capacitation. Ongoing and future studies address the mechanisms by which factors from the female reproductive tract regulate these and other pathways, leading to sperm fertility.

Sperm-egg interaction begins when sperm contact the egg extracellular matrix (the zona pellucida). ZP3, a protein component of the zona pellucida, activates sperm and triggers a secretory event known as the acrosome reaction. This is an obligatory event in gamete interaction that must be completed if sperm are to fuse with eggs, and disruption of the zona pellucida-evoked acrosome reaction is linked to several types of male infertility. We have worked on the signal transduction cascade that is regulated by ZP3. Specifically, we have focused on the intersecting roles of Ca2+ and Ca2+ channels, of phosphatidylinositol pathways, and protein kinases in the control of the acrosome reaction.

One or more keywords matched the following items that are connected to Florman, Harvey
Item TypeName
Academic Article ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization.
Academic Article Activation of mouse sperm T-type Ca2+ channels by adhesion to the egg zona pellucida.
Academic Article Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction.
Academic Article Trp2 regulates entry of Ca2+ into mouse sperm triggered by egg ZP3.
Academic Article Activation of voltage-dependent calcium channels of mammalian sperm is required for zona pellucida-induced acrosomal exocytosis.
Academic Article A sustained increase in intracellular Ca(2+) is required for the acrosome reaction in sea urchin sperm.
Academic Article Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation.
Academic Article A perspective on the control of mammalian fertilization by egg-activated ion channels in sperm: a tale of two channels.
Academic Article Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation.
Academic Article Enkurin is a novel calmodulin and TRPC channel binding protein in sperm.
Academic Article Functional characterization of PKDREJ, a male germ cell-restricted polycystin.
Academic Article Calmodulin antagonists inhibit T-type Ca(2+) currents in mouse spermatogenic cells and the zona pellucida-induced sperm acrosome reaction.
Academic Article Coup d'oeil of sperm.
Academic Article How to attract a sperm.
Academic Article Sequential focal and global elevations of sperm intracellular Ca2+ are initiated by the zona pellucida during acrosomal exocytosis.
Academic Article A polycystin-1 controls postcopulatory reproductive selection in mice.
Academic Article Regulating the acrosome reaction.
Concept TRPC Cation Channels
Concept Ion Channels
Concept Calcium Channels
Concept TRPP Cation Channels
Concept G Protein-Coupled Inwardly-Rectifying Potassium Channels
Concept Transient Receptor Potential Channels
Academic Article Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways.
Search Criteria
  • Hyperpolarization Activated Cyclic Nucleotide Gated Channels