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Search Results to Harvey M Florman PhD

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One or more keywords matched the following properties of Florman, Harvey

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Academic Background

1972 B.A., Rutgers College
1982 Ph.D., University of Pennsylvania

Mechanisms of Fertilization

Fertilization is the process by which sperm and egg fuse and the program of development is initiated. This is controlled in mammals at two steps: 1) sperm first undergo “capacitation”, a physiological reprogramming that occurs within the female reproductive tract and that is essential for the expression of fertility; and 2) capacitated sperm interact with the egg. Our work has focused on the molecular and cellular mechanisms that control these two events. To this end, we apply a range of biophysical (single cell fluorescence imaging, patch clamp electrophysiology), biochemical and molecular techniques.

Studies of capacitation address the control of sperm signal transduction pathways that lead to fertility. Ion fluxes are important regulators of this process and we have identified key roles of sperm membrane potential and pH. In addition, we recently recognized a role of polycystic kidney disease proteins in capacitation. Ongoing and future studies address the mechanisms by which factors from the female reproductive tract regulate these and other pathways, leading to sperm fertility.

Sperm-egg interaction begins when sperm contact the egg extracellular matrix (the zona pellucida). ZP3, a protein component of the zona pellucida, activates sperm and triggers a secretory event known as the acrosome reaction. This is an obligatory event in gamete interaction that must be completed if sperm are to fuse with eggs, and disruption of the zona pellucida-evoked acrosome reaction is linked to several types of male infertility. We have worked on the signal transduction cascade that is regulated by ZP3. Specifically, we have focused on the intersecting roles of Ca2+ and Ca2+ channels, of phosphatidylinositol pathways, and protein kinases in the control of the acrosome reaction.


One or more keywords matched the following items that are connected to Florman, Harvey

Item TypeName
Academic Article ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization.
Academic Article pH regulation in mouse sperm: identification of Na(+)-, Cl(-)-, and HCO3(-)-dependent and arylaminobenzoate-dependent regulatory mechanisms and characterization of their roles in sperm capacitation.
Academic Article Activation of mouse sperm T-type Ca2+ channels by adhesion to the egg zona pellucida.
Academic Article Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction.
Academic Article Trp2 regulates entry of Ca2+ into mouse sperm triggered by egg ZP3.
Academic Article Activation of voltage-dependent calcium channels of mammalian sperm is required for zona pellucida-induced acrosomal exocytosis.
Academic Article A sustained increase in intracellular Ca(2+) is required for the acrosome reaction in sea urchin sperm.
Academic Article A perspective on the control of mammalian fertilization by egg-activated ion channels in sperm: a tale of two channels.
Academic Article The state of the union: the cell biology of fertilization.
Academic Article In the beginning: lessons from fertilization in mice and worms.
Academic Article Of fertility, cystic fibrosis and the bicarbonate ion.
Academic Article Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation.
Academic Article Enkurin is a novel calmodulin and TRPC channel binding protein in sperm.
Academic Article Calmodulin antagonists inhibit T-type Ca(2+) currents in mouse spermatogenic cells and the zona pellucida-induced sperm acrosome reaction.
Academic Article Shedding light on sperm pHertility.
Academic Article Coup d'oeil of sperm.
Academic Article How to attract a sperm.
Academic Article The regulation of acrosomal exocytosis. I. Sperm capacitation is required for the induction of acrosome reactions by the bovine zona pellucida in vitro.
Academic Article Nature of the mouse egg's receptor for sperm.
Academic Article Inhibition of in vitro fertilization of mouse eggs: 3-quinuclidinyl benzilate specifically blocks penetration of zonae pellucidae by mouse spermatozoa.
Academic Article Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion.
Academic Article Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization on capacitation-associated protein tyrosine phosphorylation.
Academic Article Sequential focal and global elevations of sperm intracellular Ca2+ are initiated by the zona pellucida during acrosomal exocytosis.
Academic Article Phosphoinositide-dependent pathways in mouse sperm are regulated by egg ZP3 and drive the acrosome reaction.
Academic Article A polycystin-1 controls postcopulatory reproductive selection in mice.
Academic Article Regulating the acrosome reaction.
Academic Article An adhesion-associated agonist from the zona pellucida activates G protein-promoted elevations of internal Ca2+ and pH that mediate mammalian sperm acrosomal exocytosis.
Academic Article Mechanisms of sperm-egg interactions: between sugars and broken bonds.
Academic Article O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity.
Academic Article The mouse egg's receptor for sperm: what is it and how does it work?
Academic Article Regulation of acrosomal exocytosis. II. The zona pellucida-induced acrosome reaction of bovine spermatozoa is controlled by extrinsic positive regulatory elements.
Academic Article Enzymatic dissection of the functions of the mouse egg's receptor for sperm.
Academic Article Mouse gamete interactions: the zona pellucida is the site of the acrosome reaction leading to fertilization in vitro.
Academic Article Caltrin, the calcium transport regulatory peptide of spermatozoa, modulates acrosomal exocytosis in response to the egg's zona pellucida.
Concept Sperm Motility
Concept Spermatozoa
Concept Sperm-Ovum Interactions
Concept Sperm Capacitation
Academic Article The flagellar protein Enkurin is required for mouse sperm motility and for transport through the female reproductive tract.

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