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Laboratory rotations are available to study p53 signaling pathway. Several projects are available, including studies of gene expression, the cell cycle, apoptosis, and tumor suppression. Targeted gene disruption approaches in mice combined with biochemical and molecular biology studies will be examined. Examples of potential rotation projects include the following:
1. Generate a triple mutant in p53 amino-terminal phosphorylation sites. The rotation would involve learning molecular biology techniques such as recombinant DNA purification, analysis, mutation, and subcloning.
2. Create cells from various genetically modified mice and analyze p53 function. The rotation would involve learning protein expression and purification techniques such as Western Blotting, protein purification, and FACS analysis.
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