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    Martin G Marinus PHD

    TitleProfessor Emeritus
    InstitutionUniversity of Massachusetts Medical School
    DepartmentBiochemistry and Molecular Pharmacology
    AddressUniversity of Massachusetts Medical School
    364 Plantation Street, LRB
    Worcester MA 01605
    Phone508-856-3330
      Other Positions
      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentBiochemistry and Molecular Pharmacology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentInterdisciplinary Graduate Program

      InstitutionUMMS - Programs, Centers and Institutes
      DepartmentBacterial Genetics and Pathogenesis

        Overview 
        Narrative

        Academic Background

        B.Sc.(Hons.), 1965, PhD, Otago, New Zealand, 1968
        American Cancer Society Faculty Research Award, 1976-1981
        Visiting Professor, University of Sussex, UK 1980-1981
        Erskine Fellow, University of Canterbury, NZ 1991
        Visiting Professor, University of Goettingen, Germany 1996-1997
        Visiting Professor, Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette 2006

        http://www.drug-resistance.net/

        Photo: Martin G. Marinus Biology of Enterohemorrhagic Escherichia coli O157:H7 and Enterovirus 933W.
          Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is among the leading causes of food- and water-borne illnesses affecting humans in the U.S., Europe, and Japan. Although most EHEC infections resolve spontaneously after 5-10 days of abdominal cramping and bloody diarrhea, approximately 2-7% of cases progress to the potentially fatal hemolytic uremic syndrome due, in part, to the production of cytotoxic Shiga toxins which are capable of promoting kidney failure.
         We found that resistance to certain antibiotics in E. coli O157:H7 occurs at a higher frequency than in E. coli K-12. Whole genome sequencing is being used to find the mutations responsible.
         In EHEC strain EDL933, the genes (stx2AB) encoding Shiga toxin are located on prophage 933W in the late region between genes Q and S and are transcribed with the late region genes from promoter PR’. Previous work showed that the ends of the genome in a free phage population could not be sequenced suggesting that they are terminally redundant. If so, then 933W should be able to carry out transduction of host genes from one bacterium to another. We showed that, indeed, this is the case thereby providing another way to transfer genetic material among EHEC strains.

         

         

        Formation of F-actin in HeLa cells by Escherichia coli 0157:H7. A HeLa cell infected with dam mutant bacteria of E. coli 0157:H7 is shown stained with DAPI (bacteria), filamentous actin stain (pedestals) and a merged image..

         

        Resources

         

        Oligonucleotide array transcription data

        Working with dam and dcm strains

        Bacterial Strains and Plasmids - no longer available

        Owing to circumstances beyond my control, bacterial strains and plasmids are no longer available. Please contact the E. coli Genetic Stock Center which may be able to help you with bacterial strains. Addgene may be able to provide plasmids.

         

        Microarray Analysis

        Microarray results show that global gene expression in a seqA mutant strain (left panel) is the same as that in a wildtype strain overproducing the Dam methyltransferase (center panel) but that neither is similar to that for the dam mutant (right panel).

         



        Bibliographic 
        selected publications
        List All   |   Timeline
        1. Marinus MG, Løbner-Olesen A. DNA Methylation. Ecosal Plus. 2014 Jun 6; 2014.
          View in: PubMed
        2. Carone BR, Xu T, Murphy KC, Marinus MG. High incidence of multiple antibiotic resistant cells in cultures of in enterohemorrhagic Escherichia coli O157:H7. Mutat Res. 2014 Jan; 759:1-8.
          View in: PubMed
        3. Groothuizen FS, Fish A, Petoukhov MV, Reumer A, Manelyte L, Winterwerp HH, Marinus MG, Lebbink JH, Svergun DI, Friedhoff P, Sixma TK. Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res. 2013 Sep; 41(17):8166-81.
          View in: PubMed
        4. Jakobsen H, Bojer MS, Marinus MG, Xu T, Struve C, Krogfelt KA, Løbner-Olesen A. The alkaloid compound harmane increases the lifespan of Caenorhabditis elegans during bacterial infection, by modulating the nematode's innate immune response. PLoS One. 2013; 8(3):e60519.
          View in: PubMed
        5. Marinus MG, Poteete AR. High efficiency generalized transduction in Escherichia coli O157:H7. F1000Res. 2013; 2.
          View in: PubMed
        6. Marinus, M.G. DNA Mismatch Repair. EcoSal-Escherichia coli and Salmonella: cellular and molecular biology. A. Böck, R. Curtiss III, J. B. Kaper, P. D. Karp, F. C. Neidhardt, T. Nyström, J. M. Slauch, C. L. Squires, and D. Ussery (ed.). 2012.
        7. Marinus MG. DNA methylation and mutator genes in Escherichia coli K-12. Mutat Res. 2010 Oct; 705(2):71-6.
          View in: PubMed
        8. Marinus MG, Casadesus J. Roles of DNA adenine methylation in host-pathogen interactions: mismatch repair, transcriptional regulation, and more. FEMS Microbiol Rev. 2009 May; 33(3):488-503.
          View in: PubMed
        9. Løbner-Olesen A, Slominska-Wojewodzka M, Hansen FG, Marinus MG. DnaC inactivation in Escherichia coli K-12 induces the SOS response and expression of nucleotide biosynthesis genes. PLoS One. 2008; 3(8):e2984.
          View in: PubMed
        10. Broadbent SE, Balbontin R, Casadesus J, Marinus MG, van der Woude M. YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica. J Bacteriol. 2007 Jun; 189(11):4325-7.
          View in: PubMed
        11. Riber L, Olsson JA, Jensen RB, Skovgaard O, Dasgupta S, Marinus MG, Løbner-Olesen A. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes Dev. 2006 Aug 1; 20(15):2121-34.
          View in: PubMed
        12. López de Saro FJ, Marinus MG, Modrich P, O'Donnell M. The beta sliding clamp binds to multiple sites within MutL and MutS. J Biol Chem. 2006 May 19; 281(20):14340-9.
          View in: PubMed
        13. Robbins-Manke JL, Zdraveski ZZ, Marinus M, Essigmann JM. Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli. J Bacteriol. 2005 Oct; 187(20):7027-37.
          View in: PubMed
        14. Løbner-Olesen A, Skovgaard O, Marinus MG. Dam methylation: coordinating cellular processes. Curr Opin Microbiol. 2005 Apr; 8(2):154-60.
          View in: PubMed
        15. Løbner-Olesen A, Marinus MG, Hansen FG. Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis. Proc Natl Acad Sci U S A. 2003 Apr 15; 100(8):4672-7.
          View in: PubMed
        16. Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 1; 31(7):1805-12.
          View in: PubMed
        17. Zdraveski ZZ, Mello JA, Marinus MG, Essigmann JM. Multiple pathways of recombination define cellular responses to cisplatin. Chem Biol. 2000 Jan; 7(1):39-50.
          View in: PubMed
        18. Marinus MG. Recombination is essential for viability of an Escherichia coli dam (DNA adenine methyltransferase) mutant. J Bacteriol. 2000 Jan; 182(2):463-8.
          View in: PubMed
        19. Rasmussen LJ, Løbner-Olesen A, Marinus MG. Growth-rate-dependent transcription initiation from the dam P2 promoter. Gene. 1995 May 19; 157(1-2):213-5.
          View in: PubMed
        20. Carraway M, Marinus MG. Repair of heteroduplex DNA molecules with multibase loops in Escherichia coli. J Bacteriol. 1993 Jul; 175(13):3972-80.
          View in: PubMed
        21. Foster PL, Marinus MG. Levels of epsilon, an essential replication subunit of Escherichia coli DNA polymerase III, are controlled by heat shock proteins. J Bacteriol. 1992 Dec; 174(23):7509-16.
          View in: PubMed
        22. Parker BO, Marinus MG. Repair of DNA heteroduplexes containing small heterologous sequences in Escherichia coli. Proc Natl Acad Sci U S A. 1992 Mar 1; 89(5):1730-4.
          View in: PubMed
        23. Boye E, Marinus MG, Løbner-Olesen A. Quantitation of Dam methyltransferase in Escherichia coli. J Bacteriol. 1992 Mar; 174(5):1682-5.
          View in: PubMed
        24. Løbner-Olesen A, Marinus MG. Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. J Bacteriol. 1992 Jan; 174(2):525-9.
          View in: PubMed
        25. Boe L, Marinus MG. Role of plasmid multimers in mutation to tetracycline resistance. Mol Microbiol. 1991 Oct; 5(10):2541-5.
          View in: PubMed
        26. Barras F, Marinus MG. Arrangement of Dam methylation sites (GATC) in the Escherichia coli chromosome. Nucleic Acids Res. 1988 Oct 25; 16(20):9821-38.
          View in: PubMed
        27. Fram RJ, Cusick PS, Wilson JM, Marinus MG. Mismatch repair of cis-diamminedichloroplatinum(II)-induced DNA damage. Mol Pharmacol. 1985 Jul; 28(1):51-5.
          View in: PubMed
        28. Peterson KR, Wertman KF, Mount DW, Marinus MG. Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon. Mol Gen Genet. 1985; 201(1):14-9.
          View in: PubMed
        29. Marinus MG, Poteete A, Arraj JA. Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12. Gene. 1984 Apr; 28(1):123-5.
          View in: PubMed
        30. Arraj JA, Marinus MG. Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene. J Bacteriol. 1983 Jan; 153(1):562-5.
          View in: PubMed
        31. Karran P, Marinus MG. Mismatch correction at O6-methylguanine residues in E. coli DNA. Nature. 1982 Apr 29; 296(5860):868-9.
          View in: PubMed
        32. McGraw BR, Marinus MG. Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12. Mol Gen Genet. 1980; 178(2):309-15.
          View in: PubMed
        33. Bale A, d'Alarcao M, Marinus MG. Characterization of DNA adenine methylation mutants of Escherichia coli K12. Mutat Res. 1979 Feb; 59(2):157-65.
          View in: PubMed
        34. Marinus MG, Konrad EB. Hyper-recombination in dam mutants of Escherichia coli K-12. Mol Gen Genet. 1976 Dec 22; 149(3):273-7.
          View in: PubMed
        35. Marinus MG, Morris NR. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res. 1975 Apr; 28(1):15-26.
          View in: PubMed
        36. Marinus MG, Morris NR. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12. J Mol Biol. 1974 May 15; 85(2):309-22.
          View in: PubMed
        37. Marinus MG, Adelberg EA. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. J Bacteriol. 1970 Dec; 104(3):1266-72.
          View in: PubMed
        38. Marinus MG, Loutit JS. Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. I. Characterisation and mapping of mutants. Genetics. 1969 Nov; 63(3):547-56.
          View in: PubMed
        39. Loutit JS, Marinus MG. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet Res. 1968 Aug; 12(1):37-44.
          View in: PubMed
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