Heinrich Gottlinger MD, PhD
|Institution||University of Massachusetts Medical School|
|Department||Molecular, Cell and Cancer Biology|
|Address||University of Massachusetts Medical School|
364 Plantation Street, LRB-526
Worcester MA 01605
|Institution||UMMS - School of Medicine|
|Department||Biochemistry and Molecular Pharmacology|
|Institution||UMMS - School of Medicine|
|Department||Program in Molecular Medicine|
|Institution||UMMS - Programs, Centers and Institutes|
|Department||Center for AIDS Research|
Heinrich Gottlinger received his M.D. from the University of Munich in 1983. From 1984 to 1989 he was a post-doctoral fellow, first at the Institute of Immunology, University of Munich, and later at the Dana-Farber Cancer Institute in Boston, where his work was funded by a stipend from the German Research Council. He became a faculty member in the Department of Pathology at Harvard in 1991, where he remained until he joined the Program in Gene Function and Expression at the University of Massachusetts Medical School in September of 2004. Dr. Gottlinger is the recipient of a National Institutes of Health Merit Award.
Molecular Biology of HIV-1
The overall goal of the lab is to understand at the molecular level how an infectious HIV-1 virus particle is formed and what cellular proteins are involved.
HIV-1 particle assembly and budding are driven by the viral Gag protein, which contains specific domains that are absolutely required for the virus to detach from the host cell. Towards the goal of understanding the function of these “late assembly” domains, we recently identified AIP1 as a cellular binding partner involved in HIV-1 budding. AIP1 is a component of the cellular class E vacuolar protein sorting (Vps) machinery, which normally functions in an endosomal budding pathway that is conserved from yeast to man.
Our results indicate that AIP1 serves to link the late assembly domains of HIV-1 and other lentiviruses to a large endosomal sorting complex called ESCRT-III. Remarkably, the release of HIV-1 and various other enveloped viruses is completely blocked in the presence of defective ESCRT-III components. We are currently using a combination of siRNA knockdown and mutagenesis techniques to determine how the assembly of the ESCRT-III complex is regulated, and how AIP1 and ESCRT-III facilitate virus budding. Additionally, we use HIV as a model system to elucidate how the human class E Vps machinery functions in protein sorting and vesicular transport.
We also study the role of Nef, a virulence factor of HIV-1 that is crucial for rapid progression to AIDS. Nef increases the intrinsic infectivity of HIV-1 progeny virions, but the mechanism is not understood. Our recent results show that Nef binds to a key regulator of endocytosis, which is required for the ability of Nef to increase viral infectivity. Since our results suggest that Nef down-modulates a cellular factor that restricts HIV-1 infectivity, we have used a high-resolution proteomics approach to compare the global protein content of wild type and mutant HIV-1 virions. This approach has yielded a number of cellular proteins that are selectively incorporated into HIV-1 particles in a manner that depends on a specific viral structural or regulatory protein.
The viral binding sites for these cellular proteins are currently being mapped, and the results will be used to construct and analyze HIV-1 mutants that lack these sites. To complement and validate these virological studies, we will use knockdown approaches to directly examine the relevance of specific host factors for the HIV-1 life cycle. We expect that these studies will provide insights not only into the function of Nef, but also into the mechanism of virus release, virus spreading via cell-to-cell transmission, and the unique ability of lentiviruses such as HIV-1 to infect non-dividing, terminally differentiated cells.
Potential Rotation Projects
1.The identification of novel binding partners for cellular proteins required for HIV-1 budding using a double tag immunoaffinity purification approach.
2.The characterization of mutant ESCRT-III components that block HIV-1 budding with regard to subcellular localization and their ability to interact with certain phosphoinositol lipids and with other proteins involved in late endosomal sorting.
3.The mapping of binding sites for cellular proteins that we have identified as specific components of HIV-1 using GST pulldown, yeast two-hybrid, and co-immunoprecipitation approaches.
4.The generation and functional characterization of mutant viral Gag proteins that fail to interact with specific cellular partners.
A postdoctoral position is available to study in this laboratory. Contact Dr. Gottlinger for additional details.
For assistance with using Profiles, please refer to the online tutorials
or contact UMMS Help Desk
or call 508-856-8643.
Click the "See All" links for more information and interactive visualizations!
People who are also in this person's primary department.