Sign in to edit your profile (add interests, mentoring, photo, etc.)
    Keywords
    Last Name
    Institution

    William E Theurkauf PhD

    TitleProfessor
    InstitutionUniversity of Massachusetts Medical School
    DepartmentProgram in Molecular Medicine
    AddressUniversity of Massachusetts Medical School
    377 Plantation Street
    Worcester MA 01605
    Phone508-856-4900
      Other Positions
      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentBiochemistry and Molecular Pharmacology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentBioinformatics and Computational Biology

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentInterdisciplinary Graduate Program

      InstitutionUMMS - Graduate School of Biomedical Sciences
      DepartmentMD/PhD Program

      InstitutionUMMS - Programs, Centers and Institutes
      DepartmentBiochemistry and Molecular Pharmacology

      InstitutionUMMS - Programs, Centers and Institutes
      DepartmentBioinformatics and Integrative Biology

      InstitutionUMMS - Programs, Centers and Institutes
      DepartmentProgram in Cell Dynamics

      InstitutionUMMS - Programs, Centers and Institutes
      DepartmentRNA Therapeutics Institute

        Overview 
        Narrative

        Academic Background

        Bill Theurkauf received his BA from Brandeis University in 1980, and his PhD in Biochemistry from Brandeis in 1988. From 1988 to 1993 he was a postdoctoral fellow in the Department of Biochemistry and Biophysics at UCSF, where he was supported by fellowships from the Damon Runyon-Walter Winchell Cancer Research Fund and NIH. From 1993 to 1998, he was a member of the faculty of the Department of Biochemistry and Cell Biology at the State University of New York at Stony Brook. In September 1998, Dr. Theurkauf joined the Program in Molecular Medicine at University of Massachusetts Medical Center as an associate professor. He is currently a professor in the Program in Molecular Medicine and Director of the Program in Cell and Developmental Dynamics

        RESEARCH INTERESTS

        The germline transmits the genetic instructions that perpetuate species, which presents unique pressures on genome maintenance systems. We’re interested in the mechanisms that maintain the integrity of the “immortal” genome during germline development, and in the developmental consequences of defects in these mechanisms.

        piRNA PRODUCTION AND FUNCTION

        Transposons and transposon fragments represent approximately half the human genome. Mobilization of these elements can lead to genetic instability and disease, but may also drive evolution and generate diversity during neurogenesis. In bilateral animals, Piwi-interacting RNAs (piRNAs) silence transposons during germline development and have a critical role in maintaining the integrity of the inherited genome. Primary piRNAs bind to PIWI clade Argonaute proteins and mediate transposon silencing. These small silencing RNAs are generated from long precursors encoded by heterochromatic clusters. Most of the piRNA processing machinery, by contrast, localizes to the perinuclear nuage. We would like to understand 1) the genetic and epigenetic mechanisms that specify clusters; 2) How transcripts from the heterochromatic piRNA clusters are directed to the biogenesis machinery/nuage, and 3) how piRNAs suppress transposition.

        Related publications:

        Klattenhoff, C. Bratu, D, P., McGinnis-Schultz, N., Koppetsch, B. S. , Cook, H. A., and Theurkauf, W. E. (2007). Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. Developmental Cell 12, 45-56.

        Li, C., Vagin, V. V., Lee, S., Xu, J., Ma, , Xi, H, Seitz, H., Horwich, M. D., Syrzycka, M., Honda, B. M., Kittler, E. L. W., Zapp, M. L., Klattenhoff, C., Schulz, N., Theurkauf, W. E., Weng, Z. and P. D. Zamore (2009). In the absence of Argonaute3, Aubergine-bound piRNAs collapse, but Piwi-bound piRNAs persist. Cell 137, 509-521.

        Klattenhoff, C., Xi, H, Li, C, Lee, S., Xu, J., Khurana, J.S., Schultz, N., Koppetsch, B. S., Nowosielska, A., Seitz, H., Zamore, P.D., Weng. Z. and William E. Theurkauf (2009). The Drosophila HP1 homologue Rhino is required for transposon silencing and piRNA production by dual strand clusters. Cell 138, 1137-1149. PMID: 19732946.

        Khurana, J. S., Xu. J., Weng, Z. and W. E. Theurkauf (2010). Distinct functions for the Drosophila piRNA pathway in genome maintenance and telomere protection. PLoS Genetics 6, e1001246.

        TRANSPOSON CONTROL AND GENOME EVOLUTION

        The piRNA pathway represents an adaptive immune system that controls the activity of mobile genetic elements. This rapidly evolving genome pathogens can arise from infectious viruses and spread through both interbreeding and poorly understood horizontal transfer mechanisms. We have recently found that introduction of P element transposons activates a broad spectrum of resident transposon families, and that silencing of the invading P element and resident elements is linked to generation of new transposon insertions in piRNA clusters that are transmitted through the germline with high fidelity. These findings indicate that adaptation to transposon invasion triggers significant structural changes in genome architecture that appear to genetically enhance silencing capacity. Ongoing studies are directed at understanding how invasion of a single transposon activates resident elements, and the role of this process in chromosome evolution.

        Related publication:

        Khurana, J. S., Wang, J., Xu, J., Koppetsch, B., Thomson, T., Nowosielska, A., Li., C., Zamore, P. D., Weng, Z., and W. E. Theurkauf (2011). Adaptation to P element transposon invasion in Drosophila melanogaster. Cell 147, 1551-1563.

        DNA DAMAGE CONTROL OF DEVELOPMENTAL PROGRESSION

        DNA damage checkpoint pathways have well-established roles in control of cell division and maintenance of genome integrity. Recent studies from a number of laboratories indicate that complex developmental processes are also regulated in response to DNA damage. In Drosophila, the axes of the embryo are specified through asymmetric localization of morphogenetic RNAs in the developing oocyte. During early embryogenesis, the maternally supplied RNAs that drive initial development are degraded and the genome of the zygotic is transcriptionally activated at the maternal-zygotic transition (MZT), which represents a switch in genetic control of development from the mother to the zygote. Axis specification and the MZT are controlled by DNA damage signaling through Chk2 kinase, which functions as a tumor suppressor in humans. We would like to understand how Chk2 governs these key developmental processes.

        Related publications:

        Klattenhoff, C. Bratu, D, P., McGinnis-Schultz, N., Koppetsch, B. S. , Cook, H. A., and Theurkauf, W. E. (2007). Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. Developmental Cell 12, 45-56.

        Benoit, B., He, C. H., Zhang, F., Votruba, S. M., Tadros, W., Weswood, J. T., Smibert, C. A., Lipshitz, H. D., and W. E. Theurkauf (2009). An essential role for the RNA-binding protein SMAUG at the Drosophila maternal-to-zygotic transition. Development 136, 923-932.

        Rotation Projects

        Rotation Projects

        Proper control of cell division and accurate chromosome segregation are fundamental to cell function and normal development. Chromosome segregation errors lead to birth defects, and abnormal cell division control is associated with essentially all cancers. A major aim of research in the laboratory is to understand cell cycle control and chromosome segregation mechanisms. We use a combination of classical and molecular genetics, high-resolution in vivo imaging, and biochemical techniques to define pathways that control the cell cycle and chromosome segregation in response to environmental insult (DNA damaging agents) and developmental queues. Rotation projects focus on the role of cell cycle checkpoint and tumor suppressor pathways during the earliest stages of embryogenesis, and cell cycle control of actin and microtubule reorganization during mitosis. Through these projects, students gain exposure of the art in vivo imaging and genetic and molecular manipulations of gene function to define pathways controlling cell division and chromosome segregation.

        Embryonic Patterning

        Essentially all cells are asymmetric, with structurally distinct surfaces and polarized internal organization. This asymmetry is essential to the specialized functions cells serve within complex multi-cellular organisms . A second area of interest focuses on the mechanisms that establish cellular asymmetry. In Drosophila, the embryonic axes are specified during oogenesis through the asymmetric localization of key morphogenetic molecules within the developing oocyte. We use axis specification in the fly as a model for the processes that establish cellular asymmetry. An intact microtubule network is essential to axis specification in the fly oocyte and to polarization of somatic. We hope to define the molecular functions for microtubules in establishing cellular asymmetry. We are currently using in vivo imaging techniques to directly characterize the microtubule dependent mRNA transport processes that differentiate the anterior and posterior poles of the developing oocyte. In addition, classical genetic and biochemical techniques are used to identify the microtubule motors and associated proteins that mediate mRNA movements to the oocyte poles.



        Post Docs

        A postdoctoral position is available to study in this laboratory. Contact Dr. Theurkauf for additional details.

        Bibliographic 
        selected publications
        List All   |   Timeline
        1. Zhang Z, Wang J, Schultz N, Zhang F, Parhad SS, Tu S, Vreven T, Zamore PD, Weng Z, Theurkauf WE. The HP1 Homolog Rhino Anchors a Nuclear Complex that Suppresses piRNA Precursor Splicing. Cell. 2014 Jun 5; 157(6):1353-63.
          View in: PubMed
        2. Zhuang J, Wang J, Theurkauf W, Weng Z. TEMP: a computational method for analyzing transposable element polymorphism in populations. Nucleic Acids Res. 2014 Jul 1; 42(11):6826-38.
          View in: PubMed
        3. Zhang Z, Koppetsch BS, Wang J, Tipping C, Weng Z, Theurkauf WE, Zamore PD. Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin. EMBO J. 2014 Mar 18; 33(6):536-9.
          View in: PubMed
        4. Perrat PN, DasGupta S, Wang J, Theurkauf W, Weng Z, Rosbash M, Waddell S. Transposition-driven genomic heterogeneity in the Drosophila brain. Science. 2013 Apr 5; 340(6128):91-5.
          View in: PubMed
        5. Simkin A, Wong A, Poh YP, Theurkauf WE, Jensen JD. Recurrent and recent selective sweeps in the piRNA pathway. Evolution. 2013 Apr; 67(4):1081-90.
          View in: PubMed
        6. Zhang Z, Theurkauf WE, Weng Z, Zamore PD. Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection. Silence. 2012; 3(1):9.
          View in: PubMed
        7. Zhang F, Wang J, Xu J, Zhang Z, Koppetsch BS, Schultz N, Vreven T, Meignin C, Davis I, Zamore PD, Weng Z, Theurkauf WE. UAP56 couples piRNA clusters to the perinuclear transposon silencing machinery. Cell. 2012 Nov 9; 151(4):871-84.
          View in: PubMed
        8. Khurana JS, Wang J, Xu J, Koppetsch BS, Thomson TC, Nowosielska A, Li C, Zamore PD, Weng Z, Theurkauf WE. Adaptation to P element transposon invasion in Drosophila melanogaster. Cell. 2011 Dec 23; 147(7):1551-63.
          View in: PubMed
        9. Zhang Z, Xu J, Koppetsch BS, Wang J, Tipping C, Ma S, Weng Z, Theurkauf WE, Zamore PD. Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains. Mol Cell. 2011 Nov 18; 44(4):572-84.
          View in: PubMed
        10. Khurana JS, Xu J, Weng Z, Theurkauf WE. Distinct functions for the Drosophila piRNA pathway in genome maintenance and telomere protection. PLoS Genet. 2010; 6(12):e1001246.
          View in: PubMed
        11. Khurana JS, Theurkauf W. piRNAs, transposon silencing, and Drosophila germline development. J Cell Biol. 2010 Nov 29; 191(5):905-13.
          View in: PubMed
        12. Theurkauf W. William Theurkauf. Curr Biol. 2010 May 11; 20(9):R389-90.
          View in: PubMed
        13. Varmark H, Kwak S, Theurkauf WE. A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells. Cell Cycle. 2010 Jan 15; 9(2):312-20.
          View in: PubMed
        14. Varmark H, Sparks CA, Nordberg JJ, Koppetsch BS, Theurkauf WE. DNA damage-induced cell death is enhanced by progression through mitosis. Cell Cycle. 2009 Sep 15; 8(18):2951-63.
          View in: PubMed
        15. Klattenhoff C, Xi H, Li C, Lee S, Xu J, Khurana JS, Zhang F, Schultz N, Koppetsch BS, Nowosielska A, Seitz H, Zamore PD, Weng Z, Theurkauf WE. The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters. Cell. 2009 Sep 18; 138(6):1137-49.
          View in: PubMed
        16. Li C, Vagin VV, Lee S, Xu J, Ma S, Xi H, Seitz H, Horwich MD, Syrzycka M, Honda BM, Kittler EL, Zapp ML, Klattenhoff C, Schulz N, Theurkauf WE, Weng Z, Zamore PD. Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell. 2009 May 1; 137(3):509-21.
          View in: PubMed
        17. Benoit B, He CH, Zhang F, Votruba SM, Tadros W, Westwood JT, Smibert CA, Lipshitz HD, Theurkauf WE. An essential role for the RNA-binding protein Smaug during the Drosophila maternal-to-zygotic transition. Development. 2009 Mar; 136(6):923-32.
          View in: PubMed
        18. Blumenstiel JP, Fu R, Theurkauf WE, Hawley RS. Components of the RNAi machinery that mediate long-distance chromosomal associations are dispensable for meiotic and early somatic homolog pairing in Drosophila melanogaster. Genetics. 2008 Nov; 180(3):1355-65.
          View in: PubMed
        19. Klattenhoff C, Theurkauf W. Biogenesis and germline functions of piRNAs. Development. 2008 Jan; 135(1):3-9.
          View in: PubMed
        20. Takada S, Kwak S, Koppetsch BS, Theurkauf WE. grp (chk1) replication-checkpoint mutations and DNA damage trigger a Chk2-dependent block at the Drosophila midblastula transition. Development. 2007 May; 134(9):1737-44.
          View in: PubMed
        21. Klattenhoff C, Bratu DP, McGinnis-Schultz N, Koppetsch BS, Cook HA, Theurkauf WE. Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. Dev Cell. 2007 Jan; 12(1):45-55.
          View in: PubMed
        22. Theurkauf WE, Klattenhoff C, Bratu DP, McGinnis-Schultz N, Koppetsch BS, Cook HA. rasiRNAs, DNA damage, and embryonic axis specification. Cold Spring Harb Symp Quant Biol. 2006; 71:171-80.
          View in: PubMed
        23. Serbus LR, Cha BJ, Theurkauf WE, Saxton WM. Dynein and the actin cytoskeleton control kinesin-driven cytoplasmic streaming in Drosophila oocytes. Development. 2005 Aug; 132(16):3743-52.
          View in: PubMed
        24. Förstemann K, Tomari Y, Du T, Vagin VV, Denli AM, Bratu DP, Klattenhoff C, Theurkauf WE, Zamore PD. Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. PLoS Biol. 2005 Jul; 3(7):e236.
          View in: PubMed
        25. Doxsey S, McCollum D, Theurkauf W. Centrosomes in cellular regulation. Annu Rev Cell Dev Biol. 2005; 21:411-34.
          View in: PubMed
        26. Cook HA, Koppetsch BS, Wu J, Theurkauf WE. The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification. Cell. 2004 Mar 19; 116(6):817-29.
          View in: PubMed
        27. Tomari Y, Du T, Haley B, Schwarz DS, Bennett R, Cook HA, Koppetsch BS, Theurkauf WE, Zamore PD. RISC assembly defects in the Drosophila RNAi mutant armitage. Cell. 2004 Mar 19; 116(6):831-41.
          View in: PubMed
        28. Takada S, Kelkar A, Theurkauf WE. Drosophila checkpoint kinase 2 couples centrosome function and spindle assembly to genomic integrity. Cell. 2003 Apr 4; 113(1):87-99.
          View in: PubMed
        29. Arn EA, Cha BJ, Theurkauf WE, Macdonald PM. Recognition of a bicoid mRNA localization signal by a protein complex containing Swallow, Nod, and RNA binding proteins. Dev Cell. 2003 Jan; 4(1):41-51.
          View in: PubMed
        30. Cha BJ, Serbus LR, Koppetsch BS, Theurkauf WE. Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posterior. Nat Cell Biol. 2002 Aug; 4(8):592-8.
          View in: PubMed
        31. Stevenson V, Hudson A, Cooley L, Theurkauf WE. Arp2/3-dependent pseudocleavage [correction of psuedocleavage] furrow assembly in syncytial Drosophila embryos. Curr Biol. 2002 Apr 30; 12(9):705-11.
          View in: PubMed
        32. Cha BJ, Koppetsch BS, Theurkauf WE. In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway. Cell. 2001 Jul 13; 106(1):35-46.
          View in: PubMed
        33. Richter JD, Theurkauf WE. Development. The message is in the translation. Science. 2001 Jul 6; 293(5527):60-2.
          View in: PubMed
        34. Theurkauf WE. TACCing down the spindle poles. Nat Cell Biol. 2001 Jul; 3(7):E159-61.
          View in: PubMed
        35. Stevenson VA, Kramer J, Kuhn J, Theurkauf WE. Centrosomes and the Scrambled protein coordinate microtubule-independent actin reorganization. Nat Cell Biol. 2001 Jan; 3(1):68-75.
          View in: PubMed
        36. Guilherme A, Emoto M, Buxton JM, Bose S, Sabini R, Theurkauf WE, Leszyk J, Czech MP. Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes. J Biol Chem. 2000 Dec 8; 275(49):38151-9.
          View in: PubMed
        37. Groisman I, Huang YS, Mendez R, Cao Q, Theurkauf W, Richter JD. CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division. Cell. 2000 Oct 27; 103(3):435-47.
          View in: PubMed
        38. Stevenson VA, Theurkauf WE. Actin cytoskeleton: putting a CAP on actin polymerization. Curr Biol. 2000 Oct 5; 10(19):R695-7.
          View in: PubMed
        39. Sibon OC, Kelkar A, Lemstra W, Theurkauf WE. DNA-replication/DNA-damage-dependent centrosome inactivation in Drosophila embryos. Nat Cell Biol. 2000 Feb; 2(2):90-5.
          View in: PubMed
        40. Sibon OC, Laurençon A, Hawley R, Theurkauf WE. The Drosophila ATM homologue Mei-41 has an essential checkpoint function at the midblastula transition. Curr Biol. 1999 Mar 25; 9(6):302-12.
          View in: PubMed
        41. Theurkauf WE, Heck MM. Identification and characterization of mitotic mutations in Drosophila. Methods Cell Biol. 1999; 61:317-46.
          View in: PubMed
        42. Theurkauf WE, Hazelrigg TI. In vivo analyses of cytoplasmic transport and cytoskeletal organization during Drosophila oogenesis: characterization of a multi-step anterior localization pathway. Development. 1998 Sep; 125(18):3655-66.
          View in: PubMed
        43. Theurkauf WE. Oocyte differentiation: a motor makes a difference. Curr Biol. 1997 Sep 1; 7(9):R548-51.
          View in: PubMed
        44. Sibon OC, Stevenson VA, Theurkauf WE. DNA-replication checkpoint control at the Drosophila midblastula transition. Nature. 1997 Jul 3; 388(6637):93-7.
          View in: PubMed
        45. Matthies HJ, McDonald HB, Goldstein LS, Theurkauf WE. Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein. J Cell Biol. 1996 Jul; 134(2):455-64.
          View in: PubMed
        46. Lieberfarb ME, Chu T, Wreden C, Theurkauf W, Gergen JP, Strickland S. Mutations that perturb poly(A)-dependent maternal mRNA activation block the initiation of development. Development. 1996 Feb; 122(2):579-88.
          View in: PubMed
        47. Sullivan W, Theurkauf WE. The cytoskeleton and morphogenesis of the early Drosophila embryo. Curr Opin Cell Biol. 1995 Feb; 7(1):18-22.
          View in: PubMed
        48. Cooley L, Theurkauf WE. Cytoskeletal functions during Drosophila oogenesis. Science. 1994 Oct 28; 266(5185):590-6.
          View in: PubMed
        49. Theurkauf WE. Microtubules and cytoplasm organization during Drosophila oogenesis. Dev Biol. 1994 Oct; 165(2):352-60.
          View in: PubMed
        50. Theurkauf WE. Premature microtubule-dependent cytoplasmic streaming in cappuccino and spire mutant oocytes. Science. 1994 Sep 30; 265(5181):2093-6.
          View in: PubMed
        51. Theurkauf WE. Actin cytoskeleton. Through the bottleneck. Curr Biol. 1994 Jan 1; 4(1):76-8.
          View in: PubMed
        52. Theurkauf WE. Immunofluorescence analysis of the cytoskeleton during oogenesis and early embryogenesis. Methods Cell Biol. 1994; 44:489-505.
          View in: PubMed
        53. Hawley RS, Theurkauf WE. Requiem for distributive segregation: achiasmate segregation in Drosophila females. Trends Genet. 1993 Sep; 9(9):310-7.
          View in: PubMed
        54. Theurkauf WE, Alberts BM, Jan YN, Jongens TA. A central role for microtubules in the differentiation of Drosophila oocytes. Development. 1993 Aug; 118(4):1169-80.
          View in: PubMed
        55. Sullivan W, Fogarty P, Theurkauf W. Mutations affecting the cytoskeletal organization of syncytial Drosophila embryos. Development. 1993 Aug; 118(4):1245-54.
          View in: PubMed
        56. Baker J, Theurkauf WE, Schubiger G. Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo. J Cell Biol. 1993 Jul; 122(1):113-21.
          View in: PubMed
        57. McKim KS, Jang JK, Theurkauf WE, Hawley RS. Mechanical basis of meiotic metaphase arrest. Nature. 1993 Mar 25; 362(6418):364-6.
          View in: PubMed
        58. Theurkauf WE. Behavior of structurally divergent alpha-tubulin isotypes during Drosophila embryogenesis: evidence for post-translational regulation of isotype abundance. Dev Biol. 1992 Nov; 154(1):205-17.
          View in: PubMed
        59. Theurkauf WE, Smiley S, Wong ML, Alberts BM. Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport. Development. 1992 Aug; 115(4):923-36.
          View in: PubMed
        60. Theurkauf WE, Hawley RS. Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein. J Cell Biol. 1992 Mar; 116(5):1167-80.
          View in: PubMed
        61. Kellogg DR, Sullivan W, Theurkauf W, Oegema K, Raff JW, Alberts BM. Studies on the centrosome and cytoplasmic organization in the early Drosophila embryo. Cold Spring Harb Symp Quant Biol. 1991; 56:649-62.
          View in: PubMed
        62. Theurkauf WE, Baum H, Bo J, Wensink PC. Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins. Proc Natl Acad Sci U S A. 1986 Nov; 83(22):8477-81.
          View in: PubMed
        63. Vallee RB, Bloom GS, Theurkauf WE. Microtubule-associated proteins: subunits of the cytomatrix. J Cell Biol. 1984 Jul; 99(1 Pt 2):38s-44s.
          View in: PubMed
        64. De Camilli P, Miller PE, Navone F, Theurkauf WE, Vallee RB. Distribution of microtubule-associated protein 2 in the nervous system of the rat studied by immunofluorescence. Neuroscience. 1984 Apr; 11(4):817-46.
          View in: PubMed
        65. Theurkauf WE, Vallee RB. Extensive cAMP-dependent and cAMP-independent phosphorylation of microtubule-associated protein 2. J Biol Chem. 1983 Jun 25; 258(12):7883-6.
          View in: PubMed
        66. Miller P, Walter U, Theurkauf WE, Vallee RB, De Camilli P. Frozen tissue sections as an experimental system to reveal specific binding sites for the regulatory subunit of type II cAMP-dependent protein kinase in neurons. Proc Natl Acad Sci U S A. 1982 Sep; 79(18):5562-6.
          View in: PubMed
        67. Theurkauf WE, Vallee RB. Molecular characterization of the cAMP-dependent protein kinase bound to microtubule-associated protein 2. J Biol Chem. 1982 Mar 25; 257(6):3284-90.
          View in: PubMed
        68. Vallee RB, DiBartolomeis MJ, Theurkauf WE. A protein kinase bound to the projection portion of MAP 2 (microtubule-associated protein 2). J Cell Biol. 1981 Sep; 90(3):568-76.
          View in: PubMed
        For assistance with using Profiles, please refer to the online tutorials or contact UMMS Help Desk or call 508-856-8643.
        William's Networks
        Click the "See All" links for more information and interactive visualizations!
        Concepts
        _
        Co-Authors
        _
        Similar People
        _
        Same Department
        Physical Neighbors
        _

        This is an official Page/Publication of the University of Massachusetts Worcester Campus
        Office of the Vice Provost for Research, 55 Lake Ave North, Worcester, Massachusetts 01655
        Questions or Comments? Email: publicaffairs@umassmed.edu Phone: 508-856-1572