"Ligase Chain Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
|Ligase Chain Reaction
- Ligase Chain Reaction
- Chain Reaction, Ligase
- Chain Reactions, Ligase
- Ligase Chain Reactions
- Reaction, Ligase Chain
- Reactions, Ligase Chain
Below are MeSH descriptors whose meaning is more general than "Ligase Chain Reaction".
Below are MeSH descriptors whose meaning is more specific than "Ligase Chain Reaction".
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Below are the most recent publications written about "Ligase Chain Reaction" by people in Profiles.
Ong C, Tai W, Sarma A, Opal SM, Artenstein AW, Tripathi A. Ligation with nucleic acid sequence-based amplification. J Mol Diagn. 2012 May-Jun; 14(3):206-13.
Braverman PK, Schwarz DF, Mph M, Deforest A, Hodinka RL, McGowan KL, Mortensen JE. Use of ligase chain reaction for laboratory identification of Chlamydia trachomatis and Neisseria gonorrhoeae in adolescent women. J Pediatr Adolesc Gynecol. 2002 Feb; 15(1):37-41.