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Joshua Jay Singer PhD

TitleProfessor
InstitutionUniversity of Massachusetts Medical School
DepartmentMicrobiology and Physiological Systems
AddressUniversity of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Phone508-856-6694
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionUMMS - School of Medicine
    DepartmentMicrobiology and Physiological Systems

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentNeuroscience


    Collapse Biography 
    Collapse education and training
    Massachusetts Institute of Technology, Cambridge, MA, United StatesSB SM EEElectrical Engineering
    Harvard University, Cambridge, MA, United StatesPHDPhysiology

    Collapse Overview 
    Collapse overview

    Academic Background

    1966, S.B., S.M., E.E., Massachusetts Institute of Technology
    1970, Ph.D., Harvard University

    Ion channel behavior and cellular physiological and biochemical processes

    The aim of our research is to understand the molecular mechanismsinvolved in membrane electrical events and the relationship of theseevents to biochemical and physiological processes. Studies are carriedout using mammalian and amphibian single smooth muscle cells and othercell types as well as various expression systems including Xenopusoocytes. We are using voltage-clamp techniques for measuring macroscopicmembrane currents, the patch-clamp technique for measuring currentspassing through single ion channels, Ca2+imaging techniques for examining the temporal and spatial distribution ofcellular Ca2+, and molecular biologicaltechniques for studying cloned channels, identifying new channels, anddetermining various functional domains of channel proteins. We have beenexamining the voltage, chemical, mechanical, and ionic control of membranechannels; the mechanism of action of neurotransmitters, peptides, drugsand other agents; and the role of these in cellular function. Specifically, we have recently been interested in the direct effects offatty acids, sphingosine, steroids, and other biologically active lipidson smooth muscle and neuron ion channel activity; the role ofmechanically-gated ion channels in the control of stretch-inducedcontraction of smooth muscle; various Ca2+permeable nonselective cation channels activated by caffeine, NMDA andATP; and the relationship between ion channel gating and changes in thelocal concentration of cellular Ca2+.




    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Zhang YA, Tuft RA, Lifshitz LM, Fogarty KE, Singer JJ, Zou H. Caffeine-activated large-conductance plasma membrane cation channels in cardiac myocytes: characteristics and significance. Am J Physiol Heart Circ Physiol. 2007 Oct; 293(4):H2448-61. PMID: 17483243.
      View in: PubMed
    2. Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. Using total fluorescence increase (signal mass) to determine the Ca2+ current underlying localized Ca2+ events. J Gen Physiol. 2004 Sep; 124(3):259-72. PMID: 15337821.
      View in: PubMed
    3. Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. Imaging calcium entering the cytosol through a single opening of plasma membrane ion channels: SCCaFTs--fundamental calcium events. Cell Calcium. 2004 Jun; 35(6):523-33. PMID: 15110142.
      View in: PubMed
    4. Clarke AL, Petrou S, Walsh JV, Singer JJ. Modulation of BK(Ca) channel activity by fatty acids: structural requirements and mechanism of action. Am J Physiol Cell Physiol. 2002 Nov; 283(5):C1441-53. PMID: 12372805.
      View in: PubMed
    5. Clarke AL, Petrou S, Walsh JV, Singer JJ. Site of action of fatty acids and other charged lipids on BKCa channels from arterial smooth muscle cells. Am J Physiol Cell Physiol. 2003 Mar; 284(3):C607-19. PMID: 12409285.
      View in: PubMed
    6. Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. Visualization of Ca2+ entry through single stretch-activated cation channels. Proc Natl Acad Sci U S A. 2002 Apr 30; 99(9):6404-9. PMID: 11983921.
      View in: PubMed
    7. Dopico AM, Walsh JV, Singer JJ. Natural bile acids and synthetic analogues modulate large conductance Ca2+-activated K+ (BKCa) channel activity in smooth muscle cells. J Gen Physiol. 2002 Mar; 119(3):251-73. PMID: 11865021.
      View in: PubMed
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