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Stephen J Doxsey PhD

TitleProfessor
InstitutionUniversity of Massachusetts Medical School
DepartmentProgram in Molecular Medicine
AddressUniversity of Massachusetts Medical School
373 Plantation Street, Two Biotech, Suite 210
Worcester MA 01605
Phone508-856-1613
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionUMMS - School of Medicine
    DepartmentProgram in Molecular Medicine

    InstitutionUMMS - School of Medicine
    DepartmentRadiology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentCancer Biology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentCell Biology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentTranslational Science


    Collapse Biography 
    Collapse education and training
    University of Connecticut, Storrs, CT, United StatesBABiology
    Yale University, New Haven, CT, United StatesPHDCell Biology

    Collapse Overview 
    Collapse overview

    Academic Background

    Stephen Doxsey received his BS from the University of Connecticut in 1977 and his PhD in Cell Biology from Yale University in 1987. He was awarded Anna Fuller and Damon Runyon Fellowships during his postdoctoral training in Dr. Marc Kirschner's laboratory at the University of California at San Francisco. Dr. Doxsey joined the faculty at the University of Massachusetts Medical School in the Program in Molecular Medicine in 1993.

    Regulation of Mitotic Spindle Assembly

    Steve DoxseyMicrotubules are required for such fundamental cellular processes as cell division, embryogenesis, cell motility and organelle translocation. In all animal cells, the microtubule cytoskeleton arises from the centrosome, an organelle with the ability to nucleate microtubules.

    This laboratory is interested in understanding how the centrosome organizes microtubule arrays such as the mitotic spindle. We have cloned and characterized pericentrin, a highly conserved centrosome protein essential for cell division and microtubule organization. Pericentrin forms a large complex with gamma tubulin and other proteins involved in microtubule nucleation. Expression of mutated forms of pericentrin in cells induces the formation of 'ectopic centrosomes' that nucleate microtubules. Current work involves expression of chimeras of pericentrin and green fluorescent protein to monitor centrosome dynamics and function in living cells. In collaboration with the Biomedical Imaging Group, who developed new superresolution immunofluorescence technology, we discovered that centrosomes are comprised of a novel lattice structure (see Figure A). The mechanism and regulation of centrosome assembly is being investigated using in vitro reconstitution systems and in vivo systems developed in our laboratory. Centrosome assembly in mammalian cells is microtubule-dependent and mediated by the molecular motor, dynein. We are characterizing other highly conserved, novel centrosome proteins identified with serum from patients with the autoimmune disease, scleroderma. Finally, we recently discovered that pericentrin is overexpressed in tumors and that tumor cells have abnormal centrosomes, suggesting a possible role of pericentrin in tumorigenesis.

    Figure

    Research Figure A.


    A. Immunofluorescence image of microtubules (red) grown from a centrosome (yellow). The centrosome was stained with antibodies to pericentrin, a highly conserved and essential centrosome protein. The novel centrosome lattice revealed in this image was discovered using imaging technology recently developed by Dr. Fred Fay and coworkers here at UMass.



    Collapse Rotation Projects

    Potential Rotation Projects:

    Project #1: Centrosomes, chromosome segregation and cancer. We have shown that the assembly of centrosomes is essential for spindle function (Zimmerman et al., 1999, 2000; Doxsey, 2001). We recently discovered that centrosomes assemble by an unexpected mechanism involving the molecular motor cytoplasmic dynein (Young et al, 2000; Tynan et al., 2000). This mechanism is mediated by a direct interaction between the essential centrosome protein pericentrin (Doxsey et al., 1994; Dictenberg et al., 1998) and the dynein light intermediate chain (Purohit et al., 1999; Tynana et al., 2000). Moreover, pericentrin interacts with many proteins including the g tubulin complex, which nucleates microtubules (Dictenberg et al., 1998; Diviani et al., 2000). Centrosome assembly is disrupted in human cancer--nearly all malignant tumors have aberrant centrosomes (Pihan et al., 1998, Doxsey, 1999). To our surprise, most tumor cells overexpress pericentrin and artificial overexpression of pericentrin in normal cells induces a cancer-like phenotype (Purohit et al., 1999; Pihan et al., 2000, 2001). Projects:

    1. Determine how centrosome assembly is regulated.
    2. Determine the role of the pericentrin-dynein interaction in spindle organization.
    3. Test how increased pericentrin levels and centrosome defects contribute to cancer.
    4. Identify novel pericentrin-binding proteins.
    5. Determine how pericentrin levels and functions are regulated.
    6. Determine how pericentrin binds and transports g tubulin complexes to centrosomes.

    Project #2: Mechanism of autoantibody production in autoimmunity. Centrosomes are targeted by autoantibodies in the human autoimmune disease scleroderma (Gavanescu et al., 1999), although the mechanism is unknown. We recently discovered that centrosome autoantibodies can be induced an infectious agent (Gavanescu et al., in prep). PCR analysis demonstrated ectopic localization of this organism to skin lesions of scleroderma patients but not controls. These results suggest that this agent contributes to autoantibody development, disease pathogenesis and immune dysfunction in scleroderma patients. Projects:

    1. Determine the mechanism of autoantibody production in mice mutant for immune components.
    2. Test whether centrosome autoreactivity precedes other autoantibodies (anti-nuclear).
    3. Determine if this agent is present in other autoimmune diseases.
    4. Determine if the pathological symptoms of scleroderma are alleviated following treatment of the agent.



    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Vertii A, Hehnly H, Doxsey S. The Centrosome, a Multitalented Renaissance Organelle. Cold Spring Harb Perspect Biol. 2016 Dec 01; 8(12). PMID: 27908937.
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    2. Vertii A, Ivshina M, Zimmerman W, Hehnly H, Kant S, Doxsey S. The Centrosome Undergoes Plk1-Independent Interphase Maturation during Inflammation and Mediates Cytokine Release. Dev Cell. 2016 May 23; 37(4):377-86. PMID: 27219065.
      View in: PubMed
    3. Hung HF, Hehnly H, Doxsey S. The Mother Centriole Appendage Protein Cenexin Modulates Lumen Formation through Spindle Orientation. Curr Biol. 2016 May 09; 26(9):1248. PMID: 27166686.
      View in: PubMed
    4. Vertii A, Hung HF, Hehnly H, Doxsey S. Human basal body basics. Cilia. 2016; 5:13. PMID: 26981235.
      View in: PubMed
    5. Hung HF, Hehnly H, Doxsey S. The Mother Centriole Appendage Protein Cenexin Modulates Lumen Formation through Spindle Orientation. Curr Biol. 2016 Mar 21; 26(6):793-801. PMID: 26948879.
      View in: PubMed
    6. Cortez BA, Rezende-Teixeira P, Redick S, Doxsey S, Machado-Santelli GM. Multipolar mitosis and aneuploidy after chrysotile treatment: a consequence of abscission failure and cytokinesis regression. Oncotarget. 2016 Feb 23; 7(8):8979-92. PMID: 26788989.
      View in: PubMed
    7. Vertii A, Bright A, Delaval B, Hehnly H, Doxsey S. New frontiers: discovering cilia-independent functions of cilia proteins. EMBO Rep. 2015 Oct; 16(10):1275-87. PMID: 26358956.
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    8. Vertii A, Zimmerman W, Ivshina M, Doxsey S. Centrosome-intrinsic mechanisms modulate centrosome integrity during fever. Mol Biol Cell. 2015 Oct 01; 26(19):3451-63. PMID: 26269579.
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    9. Hung HF, Hehnly H, Doxsey S. Methods to analyze novel liaisons between endosomes and centrosomes. Methods Cell Biol. 2015; 130:47-58. PMID: 26360027.
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    10. Waters AM, Asfahani R, Carroll P, Bicknell L, Lescai F, Bright A, Chanudet E, Brooks A, Christou-Savina S, Osman G, Walsh P, Bacchelli C, Chapgier A, Vernay B, Bader DM, Deshpande C, O' Sullivan M, Ocaka L, Stanescu H, Stewart HS, Hildebrandt F, Otto E, Johnson CA, Szymanska K, Katsanis N, Davis E, Kleta R, Hubank M, Doxsey S, Jackson A, Stupka E, Winey M, Beales PL. The kinetochore protein, CENPF, is mutated in human ciliopathy and microcephaly phenotypes. J Med Genet. 2015 Mar; 52(3):147-56. PMID: 25564561.
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    11. de Souza EE, Hehnly H, Perez AM, Meirelles GV, Smetana JH, Doxsey S, Kobarg J. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle. 2015; 14(4):656-67. PMID: 25664600.
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    12. Ivshina M, Alexandrov IM, Vertii A, Doxsey S, Richter JD. CPEB regulation of TAK1 synthesis mediates cytokine production and the inflammatory immune response. Mol Cell Biol. 2015 Feb; 35(3):610-8. PMID: 25452303.
      View in: PubMed
    13. Chen CT, Hehnly H, Yu Q, Farkas D, Zheng G, Redick SD, Hung HF, Samtani R, Jurczyk A, Akbarian S, Wise C, Jackson A, Bober M, Guo Y, Lo C, Doxsey S. A unique set of centrosome proteins requires pericentrin for spindle-pole localization and spindle orientation. Curr Biol. 2014 Oct 06; 24(19):2327-34. PMID: 25220058.
      View in: PubMed
    14. de Souza EE, Meirelles GV, Godoy BB, Perez AM, Smetana JH, Doxsey SJ, McComb ME, Costello CE, Whelan SA, Kobarg J. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res. 2014 Sep 05; 13(9):4074-90. PMID: 25093993.
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    15. Das S, Hehnly H, Doxsey S. A new role for Rab GTPases during early mitotic stages. Small GTPases. 2014; 5. PMID: 24921241.
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    16. Hehnly H, Doxsey S. Rab11 endosomes contribute to mitotic spindle organization and orientation. Dev Cell. 2014 Mar 10; 28(5):497-507. PMID: 24561039.
      View in: PubMed
    17. Hehnly H, Hung HF, Doxsey S. One among many: ODF2 isoform 9, a.k.a. Cenexin-1, is required for ciliogenesis. Cell Cycle. 2013 Apr 1; 12(7):1021. PMID: 23511168.
      View in: PubMed
    18. Chen CT, Ettinger AW, Huttner WB, Doxsey SJ. Resurrecting remnants: the lives of post-mitotic midbodies. Trends Cell Biol. 2013 Mar; 23(3):118-28. PMID: 23245592.
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    19. Hung HF, Hehnly H, Doxsey S. Multiplying madly: deacetylases take charge of centrosome duplication and amplification. Cell Cycle. 2012 Dec 1; 11(23):4304. PMID: 23159860.
      View in: PubMed
    20. Chen CT, Doxsey SJ. An MBoC favorite: role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP. Mol Biol Cell. 2012 Oct; 23(19):3775. PMID: 23024182.
      View in: PubMed
    21. Hehnly H, Chen CT, Powers CM, Liu HL, Doxsey S. The centrosome regulates the Rab11- dependent recycling endosome pathway at appendages of the mother centriole. Curr Biol. 2012 Oct 23; 22(20):1944-50. PMID: 22981775.
      View in: PubMed
    22. Foraker AB, Camus SM, Evans TM, Majeed SR, Chen CY, Taner SB, Corrêa IR, Doxsey SJ, Brodsky FM. Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG. J Cell Biol. 2012 Aug 20; 198(4):591-605. PMID: 22891263.
      View in: PubMed
    23. Chen CT, Hehnly H, Doxsey SJ. Orchestrating vesicle transport, ESCRTs and kinase surveillance during abscission. Nat Rev Mol Cell Biol. 2012 Jul 11; 13(8):483-8. PMID: 22781903.
      View in: PubMed
    24. Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, Ahn HJ, Ait-Mohamed O, Ait-Si-Ali S, Akematsu T, Akira S, Al-Younes HM, Al-Zeer MA, Albert ML, Albin RL, Alegre-Abarrategui J, Aleo MF, Alirezaei M, Almasan A, Almonte-Becerril M, Amano A, Amaravadi R, Amarnath S, Amer AO, Andrieu-Abadie N, Anantharam V, Ann DK, Anoopkumar-Dukie S, Aoki H, Apostolova N, Arancia G, Aris JP, Asanuma K, Asare NY, Ashida H, Askanas V, Askew DS, Auberger P, Baba M, Backues SK, Baehrecke EH, Bahr BA, Bai XY, Bailly Y, Baiocchi R, Baldini G, Balduini W, Ballabio A, Bamber BA, Bampton ET, Bánhegyi G, Bartholomew CR, Bassham DC, Bast RC, Batoko H, Bay BH, Beau I, Béchet DM, Begley TJ, Behl C, Behrends C, Bekri S, Bellaire B, Bendall LJ, Benetti L, Berliocchi L, Bernardi H, Bernassola F, Besteiro S, Bhatia-Kissova I, Bi X, Biard-Piechaczyk M, Blum JS, Boise LH, Bonaldo P, Boone DL, Bornhauser BC, Bortoluci KR, Bossis I, Bost F, Bourquin JP, Boya P, Boyer-Guittaut M, Bozhkov PV, Brady NR, Brancolini C, Brech A, Brenman JE, Brennand A, Bresnick EH, Brest P, Bridges D, Bristol ML, Brookes PS, Brown EJ, Brumell JH, Brunetti-Pierri N, Brunk UT, Bulman DE, Bultman SJ, Bultynck G, Burbulla LF, Bursch W, Butchar JP, Buzgariu W, Bydlowski SP, Cadwell K, Cahová M, Cai D, Cai J, Cai Q, Calabretta B, Calvo-Garrido J, Camougrand N, Campanella M, Campos-Salinas J, Candi E, Cao L, Caplan AB, Carding SR, Cardoso SM, Carew JS, Carlin CR, Carmignac V, Carneiro LA, Carra S, Caruso RA, Casari G, Casas C, Castino R, Cebollero E, Cecconi F, Celli J, Chaachouay H, Chae HJ, Chai CY, Chan DC, Chan EY, Chang RC, Che CM, Chen CC, Chen GC, Chen GQ, Chen M, Chen Q, Chen SS, Chen W, Chen X, Chen X, Chen X, Chen YG, Chen Y, Chen Y, Chen YJ, Chen Z, Cheng A, Cheng CH, Cheng Y, Cheong H, Cheong JH, Cherry S, Chess-Williams R, Cheung ZH, Chevet E, Chiang HL, Chiarelli R, Chiba T, Chin LS, Chiou SH, Chisari FV, Cho CH, Cho DH, Choi AM, Choi D, Choi KS, Choi ME, Chouaib S, Choubey D, Choubey V, Chu CT, Chuang TH, Chueh SH, Chun T, Chwae YJ, Chye ML, Ciarcia R, Ciriolo MR, Clague MJ, Clark RS, Clarke PG, Clarke R, Codogno P, Coller HA, Colombo MI, Comincini S, Condello M, Condorelli F, Cookson MR, Coombs GH, Coppens I, Corbalan R, Cossart P, Costelli P, Costes S, Coto-Montes A, Couve E, Coxon FP, Cregg JM, Crespo JL, Cronjé MJ, Cuervo AM, Cullen JJ, Czaja MJ, D'Amelio M, Darfeuille-Michaud A, Davids LM, Davies FE, De Felici M, de Groot JF, de Haan CA, De Martino L, De Milito A, De Tata V, Debnath J, Degterev A, Dehay B, Delbridge LM, Demarchi F, Deng YZ, Dengjel J, Dent P, Denton D, Deretic V, Desai SD, Devenish RJ, Di Gioacchino M, Di Paolo G, Di Pietro C, Díaz-Araya G, Díaz-Laviada I, Diaz-Meco MT, Diaz-Nido J, Dikic I, Dinesh-Kumar SP, Ding WX, Distelhorst CW, Diwan A, Djavaheri-Mergny M, Dokudovskaya S, Dong Z, Dorsey FC, Dosenko V, Dowling JJ, Doxsey S, Dreux M, Drew ME, Duan Q, Duchosal MA, Duff K, Dugail I, Durbeej M, Duszenko M, Edelstein CL, Edinger AL, Egea G, Eichinger L, Eissa NT, Ekmekcioglu S, El-Deiry WS, Elazar Z, Elgendy M, Ellerby LM, Eng KE, Engelbrecht AM, Engelender S, Erenpreisa J, Escalante R, Esclatine A, Eskelinen EL, Espert L, Espina V, Fan H, Fan J, Fan QW, Fan Z, Fang S, Fang Y, Fanto M, Fanzani A, Farkas T, Farré JC, Faure M, Fechheimer M, Feng CG, Feng J, Feng Q, Feng Y, Fésüs L, Feuer R, Figueiredo-Pereira ME, Fimia GM, Fingar DC, Finkbeiner S, Finkel T, Finley KD, Fiorito F, Fisher EA, Fisher PB, Flajolet M, Florez-McClure ML, Florio S, Fon EA, Fornai F, Fortunato F, Fotedar R, Fowler DH, Fox HS, Franco R, Frankel LB, Fransen M, Fuentes JM, Fueyo J, Fujii J, Fujisaki K, Fujita E, Fukuda M, Furukawa RH, Gaestel M, Gailly P, Gajewska M, Galliot B, Galy V, Ganesh S, Ganetzky B, Ganley IG, Gao FB, Gao GF, Gao J, Garcia L, Garcia-Manero G, Garcia-Marcos M, Garmyn M, Gartel AL, Gatti E, Gautel M, Gawriluk TR, Gegg ME, Geng J, Germain M, Gestwicki JE, Gewirtz DA, Ghavami S, et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 2012 Apr; 8(4):445-544. PMID: 22966490.
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    25. Kuo TC, Doxsey S. Fates and roles of post-mitotic midbodies beyond cytokinesis. Cell Cycle. 2012 Jan 1; 11(1):7-8. PMID: 22157094.
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    26. Hehnly H, Doxsey S. Polarity sets the stage for cytokinesis. Mol Biol Cell. 2012 Jan; 23(1):7-11. PMID: 22210846.
      View in: PubMed
    27. Delaval B, Covassin L, Lawson ND, Doxsey S. Centrin depletion causes cyst formation and other ciliopathy-related phenotypes in zebrafish. Cell Cycle. 2011 Nov 15; 10(22):3964-72. PMID: 22142866.
      View in: PubMed
    28. Kuo TC, Chen CT, Baron D, Onder TT, Loewer S, Almeida S, Weismann CM, Xu P, Houghton JM, Gao FB, Daley GQ, Doxsey S. Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity. Nat Cell Biol. 2011 Sep 11; 13(10):1214-23. PMID: 21909099.
      View in: PubMed
    29. Delaval B, Bright A, Lawson ND, Doxsey S. The cilia protein IFT88 is required for spindle orientation in mitosis. Nat Cell Biol. 2011 Apr; 13(4):461-8. PMID: 21441926.
      View in: PubMed
    30. Patzke S, Redick S, Warsame A, Murga-Zamalloa CA, Khanna H, Doxsey S, Stokke T. CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation. Mol Biol Cell. 2010 Aug 1; 21(15):2555-67. PMID: 20519441.
      View in: PubMed
    31. Delaval B, Doxsey SJ. Pericentrin in cellular function and disease. J Cell Biol. 2010 Jan 25; 188(2):181-90. PMID: 19951897.
      View in: PubMed
    32. Sivaram MV, Wadzinski TL, Redick SD, Manna T, Doxsey SJ. Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint. EMBO J. 2009 Apr 8; 28(7):902-14. PMID: 19229290.
      View in: PubMed
    33. Chen CT, Doxsey S. A last-minute rescue of trapped chromatin. Cell. 2009 Feb 6; 136(3):397-9. PMID: 19203574.
      View in: PubMed
    34. Man C, Rosa J, Yip YL, Cheung AL, Kwong YL, Doxsey SJ, Tsao SW. Id1 overexpression induces tetraploidization and multiple abnormal mitotic phenotypes by modulating aurora A. Mol Biol Cell. 2008 Jun; 19(6):2389-401. PMID: 18353975.
      View in: PubMed
    35. Delaval B, Doxsey S. Genetics. Dwarfism, where pericentrin gains stature. Science. 2008 Feb 8; 319(5864):732-3. PMID: 18258883.
      View in: PubMed
    36. Kang BH, Plescia J, Dohi T, Rosa J, Doxsey SJ, Altieri DC. Regulation of tumor cell mitochondrial homeostasis by an organelle-specific Hsp90 chaperone network. Cell. 2007 Oct 19; 131(2):257-70. PMID: 17956728.
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    37. Blazkova H, von Schubert C, Mikule K, Schwab R, Angliker N, Schmuckli-Maurer J, Fernandez PC, Doxsey S, Dobbelaere DA. The IKK inhibitor BMS-345541 affects multiple mitotic cell cycle transitions. Cell Cycle. 2007 Oct 15; 6(20):2531-40. PMID: 17704647.
      View in: PubMed
    38. Sillibourne JE, Delaval B, Redick S, Sinha M, Doxsey SJ. Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. Mol Biol Cell. 2007 Sep; 18(9):3667-80. PMID: 17626165.
      View in: PubMed
    39. Mikule K, Delaval B, Kaldis P, Jurcyzk A, Hergert P, Doxsey S. Loss of centrosome integrity induces p38-p53-p21-dependent G1-S arrest. Nat Cell Biol. 2007 Feb; 9(2):160-70. PMID: 17330329.
      View in: PubMed
    40. Parish JL, Rosa J, Wang X, Lahti JM, Doxsey SJ, Androphy EJ. The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. J Cell Sci. 2006 Dec 1; 119(Pt 23):4857-65. PMID: 17105772.
      View in: PubMed
    41. Man C, Rosa J, Lee LT, Lee VH, Chow BK, Lo KW, Doxsey S, Wu ZG, Kwong YL, Jin DY, Cheung AL, Tsao SW. Latent membrane protein 1 suppresses RASSF1A expression, disrupts microtubule structures and induces chromosomal aberrations in human epithelial cells. Oncogene. 2007 May 10; 26(21):3069-80. PMID: 17099724.
      View in: PubMed
    42. Pockwinse SM, Rajgopal A, Young DW, Mujeeb KA, Nickerson J, Javed A, Redick S, Lian JB, van Wijnen AJ, Stein JL, Stein GS, Doxsey SJ. Microtubule-dependent nuclear-cytoplasmic shuttling of Runx2. J Cell Physiol. 2006 Feb; 206(2):354-62. PMID: 16110492.
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    43. Rosa J, Canovas P, Islam A, Altieri DC, Doxsey SJ. Survivin modulates microtubule dynamics and nucleation throughout the cell cycle. Mol Biol Cell. 2006 Mar; 17(3):1483-93. PMID: 16407408.
      View in: PubMed
    44. Doxsey SJ. Molecular links between centrosome and midbody. Mol Cell. 2005 Oct 28; 20(2):170-2. PMID: 16246720.
      View in: PubMed
    45. Golubkov VS, Chekanov AV, Doxsey SJ, Strongin AY. Centrosomal pericentrin is a direct cleavage target of membrane type-1 matrix metalloproteinase in humans but not in mice: potential implications for tumorigenesis. J Biol Chem. 2005 Dec 23; 280(51):42237-41. PMID: 16251193.
      View in: PubMed
    46. Gromley A, Yeaman C, Rosa J, Redick S, Chen CT, Mirabelle S, Guha M, Sillibourne J, Doxsey SJ. Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. Cell. 2005 Oct 7; 123(1):75-87. PMID: 16213214.
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    47. Doxsey S, Zimmerman W, Mikule K. Centrosome control of the cell cycle. Trends Cell Biol. 2005 Jun; 15(6):303-11. PMID: 15953548.
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    48. Golubkov VS, Boyd S, Savinov AY, Chekanov AV, Osterman AL, Remacle A, Rozanov DV, Doxsey SJ, Strongin AY. Membrane type-1 matrix metalloproteinase (MT1-MMP) exhibits an important intracellular cleavage function and causes chromosome instability. J Biol Chem. 2005 Jul 1; 280(26):25079-86. PMID: 15878869.
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    49. Doxsey S, McCollum D, Theurkauf W. Centrosomes in cellular regulation. Annu Rev Cell Dev Biol. 2005; 21:411-34. PMID: 16212501.
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    50. Jurczyk A, Gromley A, Redick S, San Agustin J, Witman G, Pazour GJ, Peters DJ, Doxsey S. Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly. J Cell Biol. 2004 Aug 30; 166(5):637-43. PMID: 15337773.
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    51. Gavanescu I, Pihan G, Halilovic E, Szomolanyi-Tsuda E, Welsh RM, Doxsey S. Mycoplasma infection induces a scleroderma-like centrosome autoantibody response in mice. Clin Exp Immunol. 2004 Aug; 137(2):288-97. PMID: 15270845.
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    52. Zimmerman WC, Sillibourne J, Rosa J, Doxsey SJ. Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry. Mol Biol Cell. 2004 Aug; 15(8):3642-57. PMID: 15146056.
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    53. Chen D, Purohit A, Halilovic E, Doxsey SJ, Newton AC. Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis. J Biol Chem. 2004 Feb 6; 279(6):4829-39. PMID: 14594954.
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    54. Gandhi R, Bonaccorsi S, Wentworth D, Doxsey S, Gatti M, Pereira A. The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly. Mol Biol Cell. 2004 Jan; 15(1):121-31. PMID: 13679514.
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    55. Pihan G, Doxsey SJ. Mutations and aneuploidy: co-conspirators in cancer? Cancer Cell. 2003 Aug; 4(2):89-94. PMID: 12957283.
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    56. Gromley A, Jurczyk A, Sillibourne J, Halilovic E, Mogensen M, Groisman I, Blomberg M, Doxsey S. A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase. J Cell Biol. 2003 May 12; 161(3):535-45. PMID: 12732615.
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    57. Pihan GA, Wallace J, Zhou Y, Doxsey SJ. Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas. Cancer Res. 2003 Mar 15; 63(6):1398-404. PMID: 12649205.
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    58. Doxsey S. Duplicating dangerously: linking centrosome duplication and aneuploidy. Mol Cell. 2002 Sep; 10(3):439-40. PMID: 12408813.
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    59. Doxsey S. Re-evaluating centrosome function. Nat Rev Mol Cell Biol. 2001 Sep; 2(9):688-98. PMID: 11533726.
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    60. Doxsey SJ. Centrosomes as command centres for cellular control. Nat Cell Biol. 2001 May; 3(5):E105-8. PMID: 11331889.
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    61. Pihan GA, Purohit A, Wallace J, Malhotra R, Liotta L, Doxsey SJ. Centrosome defects can account for cellular and genetic changes that characterize prostate cancer progression. Cancer Res. 2001 Mar 1; 61(5):2212-9. PMID: 11280789.
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    62. Purohit A, Pihan GA, Doxsey SJ. Methods for the study of pericentrin in centrosome assembly and function. Methods Cell Biol. 2001; 67:53-69. PMID: 11550481.
      View in: PubMed
    63. Zimmerman W, Doxsey SJ. Construction of centrosomes and spindle poles by molecular motor-driven assembly of protein particles. Traffic. 2000 Dec; 1(12):927-34. PMID: 11208082.
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    64. Tynan SH, Purohit A, Doxsey SJ, Vallee RB. Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin. J Biol Chem. 2000 Oct 20; 275(42):32763-8. PMID: 10893222.
      View in: PubMed
    65. Young A, Dictenberg JB, Purohit A, Tuft R, Doxsey SJ. Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes. Mol Biol Cell. 2000 Jun; 11(6):2047-56. PMID: 10848628.
      View in: PubMed
    66. Diviani D, Langeberg LK, Doxsey SJ, Scott JD. Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain. Curr Biol. 2000 Apr 6; 10(7):417-20. PMID: 10753751.
      View in: PubMed
    67. Purohit A, Tynan SH, Vallee R, Doxsey SJ. Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization. J Cell Biol. 1999 Nov 1; 147(3):481-92. PMID: 10545494.
      View in: PubMed
    68. Pihan GA, Doxsey SJ. The mitotic machinery as a source of genetic instability in cancer. Semin Cancer Biol. 1999 Aug; 9(4):289-302. PMID: 10448116.
      View in: PubMed
    69. Gavanescu I, Vazquez-Abad D, McCauley J, Senecal JL, Doxsey S. Centrosome proteins: a major class of autoantigens in scleroderma. J Clin Immunol. 1999 May; 19(3):166-71. PMID: 10404401.
      View in: PubMed
    70. Zimmerman W, Sparks CA, Doxsey SJ. Amorphous no longer: the centrosome comes into focus. Curr Opin Cell Biol. 1999 Feb; 11(1):122-8. PMID: 10047524.
      View in: PubMed
    71. Young A, Tuft R, Carrington W, Doxsey SJ. Centrosome dynamics in living cells. Methods Cell Biol. 1999; 58:223-38. PMID: 9891384.
      View in: PubMed
    72. Bangs P, Burke B, Powers C, Craig R, Purohit A, Doxsey S. Functional analysis of Tpr: identification of nuclear pore complex association and nuclear localization domains and a role in mRNA export. J Cell Biol. 1998 Dec 28; 143(7):1801-12. PMID: 9864356.
      View in: PubMed
    73. Doxsey S. The centrosome--a tiny organelle with big potential. Nat Genet. 1998 Oct; 20(2):104-6. PMID: 9771694.
      View in: PubMed
    74. Pihan GA, Purohit A, Wallace J, Knecht H, Woda B, Quesenberry P, Doxsey SJ. Centrosome defects and genetic instability in malignant tumors. Cancer Res. 1998 Sep 1; 58(17):3974-85. PMID: 9731511.
      View in: PubMed
    75. Dictenberg JB, Zimmerman W, Sparks CA, Young A, Vidair C, Zheng Y, Carrington W, Fay FS, Doxsey SJ. Pericentrin and gamma-tubulin form a protein complex and are organized into a novel lattice at the centrosome. J Cell Biol. 1998 Apr 06; 141(1):163-74. PMID: 9531556.
      View in: PubMed
    76. Blomberg-Wirschell M, Doxsey SJ. Rapid isolation of centrosomes. Methods Enzymol. 1998; 298:228-38. PMID: 9751885.
      View in: PubMed
    77. Tucker JB, Mogensen MM, Henderson CG, Doxsey SJ, Wright M, Stearns T. Nucleation and capture of large cell surface-associated microtubule arrays that are not located near centrosomes in certain cochlear epithelial cells. J Anat. 1998 Jan; 192 ( Pt 1):119-30. PMID: 9568567.
      View in: PubMed
    78. Pockwinse SM, Krockmalnic G, Doxsey SJ, Nickerson J, Lian JB, van Wijnen AJ, Stein JL, Stein GS, Penman S. Cell cycle independent interaction of CDC2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold. Proc Natl Acad Sci U S A. 1997 Apr 1; 94(7):3022-7. PMID: 9096339.
      View in: PubMed
    79. Pereira AJ, Dalby B, Stewart RJ, Doxsey SJ, Goldstein LS. Mitochondrial association of a plus end-directed microtubule motor expressed during mitosis in Drosophila. J Cell Biol. 1997 Mar 10; 136(5):1081-90. PMID: 9060472.
      View in: PubMed
    80. Mogensen MM, Mackie JB, Doxsey SJ, Stearns T, Tucker JB. Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. Cell Motil Cytoskeleton. 1997; 36(3):276-90. PMID: 9067623.
      View in: PubMed
    81. Vidair CA, Huang RN, Doxsey SJ. Heat shock causes protein aggregation and reduced protein solubility at the centrosome and other cytoplasmic locations. Int J Hyperthermia. 1996 Sep-Oct; 12(5):681-95. PMID: 8886893.
      View in: PubMed
    82. Brown CR, Doxsey SJ, Hong-Brown LQ, Martin RL, Welch WJ. Molecular chaperones and the centrosome. A role for TCP-1 in microtubule nucleation. J Biol Chem. 1996 Jan 12; 271(2):824-32. PMID: 8557692.
      View in: PubMed
    83. Brown CR, Hong-Brown LQ, Doxsey SJ, Welch WJ. Molecular chaperones and the centrosome. A role for HSP 73 in centrosomal repair following heat shock treatment. J Biol Chem. 1996 Jan 12; 271(2):833-40. PMID: 8557693.
      View in: PubMed
    84. Sparks CA, Fey EG, Vidair CA, Doxsey SJ. Phosphorylation of NUMA occurs during nuclear breakdown and not mitotic spindle assembly. J Cell Sci. 1995 Nov; 108 ( Pt 11):3389-96. PMID: 8586651.
      View in: PubMed
    85. Shisheva A, Doxsey SJ, Buxton JM, Czech MP. Pericentriolar targeting of GDP-dissociation inhibitor isoform 2. Eur J Cell Biol. 1995 Oct; 68(2):143-58. PMID: 8575461.
      View in: PubMed
    86. Vidair CA, Doxsey SJ, Dewey WC. Thermotolerant cells possess an enhanced capacity to repair heat-induced alterations to centrosome structure and function. J Cell Physiol. 1995 Apr; 163(1):194-203. PMID: 7896896.
      View in: PubMed
    87. Brown CR, Doxsey SJ, White E, Welch WJ. Both viral (adenovirus E1B) and cellular (hsp 70, p53) components interact with centrosomes. J Cell Physiol. 1994 Jul; 160(1):47-60. PMID: 8021299.
      View in: PubMed
    88. Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell. 1994 Feb 25; 76(4):639-50. PMID: 8124707.
      View in: PubMed
    89. Vidair CA, Doxsey SJ, Dewey WC. Heat shock alters centrosome organization leading to mitotic dysfunction and cell death. J Cell Physiol. 1993 Mar; 154(3):443-55. PMID: 8436595.
      View in: PubMed
    90. Gard DL, Hafezi S, Zhang T, Doxsey SJ. Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle. J Cell Biol. 1990 Jun; 110(6):2033-42. PMID: 2190990.
      View in: PubMed
    91. Ellens H, Doxsey S, Glenn JS, White JM. Delivery of macromolecules into cells expressing a viral membrane fusion protein. Methods Cell Biol. 1989; 31:155-78. PMID: 2779447.
      View in: PubMed
    92. Doxsey SJ, Brodsky FM, Blank GS, Helenius A. Inhibition of endocytosis by anti-clathrin antibodies. Cell. 1987 Jul 31; 50(3):453-63. PMID: 3111717.
      View in: PubMed
    93. Helenius A, Doxsey S, Mellman I. Viruses as tools in drug delivery. Ann N Y Acad Sci. 1987; 507:1-6. PMID: 3126693.
      View in: PubMed
    94. Doxsey SJ, Sambrook J, Helenius A, White J. An efficient method for introducing macromolecules into living cells. J Cell Biol. 1985 Jul; 101(1):19-27. PMID: 2989298.
      View in: PubMed
    95. Moulton J, Buscher G, Bovell D, Doxsey S. Blast transformation of adherent macrophages infected in vitro with sporozoites of Theileria parva. Am J Vet Res. 1984 Apr; 45(4):678-84. PMID: 6428284.
      View in: PubMed
    96. Shapiro SZ, Doxsey SJ. Purification of nuclei from a flagellate protozoan, Trypanosoma brucei. Anal Biochem. 1982 Nov 15; 127(1):112-5. PMID: 7165080.
      View in: PubMed
    97. Fawcett DW, Doxsey S, Stagg DA, Young AS. The entry of sporozoites of Theileria parva into bovine lymphocytes in vitro. Electron microscopic observations. Eur J Cell Biol. 1982 Apr; 27(1):10-21. PMID: 6806100.
      View in: PubMed
    98. Fawcett DW, Büscher G, Doxsey S. Salivary gland of the tick vector of East Coast fever. III. The ultrastructure of sporogony in Theileria parva. Tissue Cell. 1982; 14(1):183-206. PMID: 6806940.
      View in: PubMed
    99. Fawcett DW, Büscher G, Doxsey S. Salivary gland of the tick vector of East Coast fever. IV. Cell type selectivity and host cell responses to Theileria parva. Tissue Cell. 1982; 14(2):397-414. PMID: 6810500.
      View in: PubMed
    100. Fawcett DW, Doxsey S, Büscher G. Salivary gland of the tick vector (R. appendiculatus) of East Coast fever. II. Cellular basis for fluid secretion in the type III acinus. Tissue Cell. 1981; 13(2):231-53. PMID: 7198304.
      View in: PubMed
    101. Fawcett DW, Doxsey S, Büscher G. Salivary gland of the tick vector (R. appendiculatus) of East Coast fever. I. Ultrastructure of the type III acinus. Tissue Cell. 1981; 13(2):209-30. PMID: 7198303.
      View in: PubMed
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