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Karl Dean Bellve

TitleAssistant Professor
InstitutionUMass Chan Medical School
DepartmentProgram in Molecular Medicine
AddressUMass Chan Medical School
373 Plantation Street Room 114
Worcester MA 01605
Phone508-856-6514
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentMicrobiology and Physiological Systems

    InstitutionT.H. Chan School of Medicine
    DepartmentProgram in Molecular Medicine


    Collapse Biography 
    Collapse education and training
    University of Massachusetts Amherst, Amherst, MA, United StatesBSBiochemistry
    University of Maryland Baltimore, Baltimore, MD, United StatesPHDMolecular Cellular Biology

    Collapse Overview 
    Collapse overview

    Questions about Microscopy? microscopy@umassmed.edu

    Karl Bellve is a cross-discipline scientist covering the fields of Biology, Computer Science, and Electrical Engineering. He obtained his Ph.D. in Molecular and Cellular Biology (1996, U.MD) and joined the Biomedical Imaging Group in 1997. More information about the Biomedical Imaging Group can be found from http://big.umassmed.edu.

    High-Speed 3D Epi/TIRF/Structured Light Microscopy


    His focus is on the design and implementation of computer systems and software to control  various electronics, including high-speed CCD cameras, and coordinate the high-speed electro-optical/mechanical systems, and to develop the software for specific imaging protocols necessary to gather imaging data, as well as necessary image computation and display tasks required on the control computer, and the User Interface Software that makes the system accessible. He is extremely well qualified for this role, as he designed, built and programmed the entire computer and control system for the fast TIRF/GFPM microscope system. His work has resulted in the creation of a novel high-speed fluorescence microscope system that can rapidly produce high-resolution, 3-dimensional images of fluorescence inside single cells, as well as corresponding images of near plasma membrane events at speeds of 90 images per second, using a specialized technique called Total Internal Reflection Fluorescence (TIRF) imaging, developed and implemented in collaboration with the Optical Physicists of the Biomedical Imaging Group.

    3D Deconvolution

    Additionally he has created the ability to rapidly produce 3-dimensional reconstructions of cells from 3-D images (using a process known as "deconvolution microscopy") by having developed and programmed a high-speed cluster of inexpensive computers (sometimes known as a Beowulf cluster) for this purpose.

    GPU Programming (CUDA)

    He was the first on campus to take advantage of using GPUs to exponentially speed up processing-intensive applications like 3D Deconvolution.

    Open Source Software

    A key feature of his efforts has been to contribute his software back to the scientific community. As an example, he is a significant contributor to uManager, an open source microscope acquisition package. He has contributed multiple device adapters, java classes, and bean shell scripts to the uManager code base, as well as solved difficult programming problems for the uManager community.


    Open Hardware

    Unlike Open Source Software, Open Hardware is fairly new and the basic tenets are as follows:

    "Open source hardware is hardware whose design is made publicly available so that anyone can study, modify, distribute, make, and sell the design or hardware based on that design."

    With that in mind, he recently developed an Open Hardware Focus Stabilization project called pgFocus. pg is short for "pretty good",  and is meant to poke fun at other names like Perfect Focus, Definite Focus, and Ultimate Focus.


    Focus Stabilization

    pgFocus monitors focal changes through the positional changes of a reflected laser beam.

    pgFocus Basic Implementation



    A significant feature of pgFocus is it acts as a "man-in-the-middle." It is designed to pass through faster high fidelity signals meant for a piezo Z controller while adding a slower focus control signal. This happens in the analog realm, which avoids sampling artifacts introduced by digitizing the original signal. This design also reduces the cost of pgFocus.

    pgFocus Circuit

    circuit

    pgFocus Optical Layout

     



    More information about pgFocus can be found here:

    Academic Background

    1990, B.S.,  University of Massachusetts, Amherst
    1996, Ph.D., University of Maryland, Maryland

     



    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Liao H, Sheridan T, Cosar E, Owens C, Zuo T, Wang X, Akalin A, Kandil D, Dresser K, Fogarty K, Bellve K, Baer C, Fischer A. Deconvolution microscopy: A platform for rapid on-site evaluation of fine needle aspiration specimens that enables recovery of the sample. Cytopathology. 2022 May; 33(3):312-320. PMID: 35102620.
      Citations:    Fields:    Translation:Humans
    2. Rigano A, Ehmsen S, Öztürk SU, Ryan J, Balashov A, Hammer M, Kirli K, Boehm U, Brown CM, Bellve K, Chambers JJ, Cosolo A, Coleman RA, Faklaris O, Fogarty KE, Guilbert T, Hamacher AB, Itano MS, Keeley DP, Kunis S, Lacoste J, Laude A, Ma WY, Marcello M, Montero-Llopis P, Nelson G, Nitschke R, Pimentel JA, Weidtkamp-Peters S, Park PJ, Alver BH, Grunwald D, Strambio-De-Castillia C. Author Correction: Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications. Nat Methods. 2022 Feb; 19(2):256. PMID: 34952955.
      Citations:    Fields:    
    3. Rigano A, Ehmsen S, Öztürk SU, Ryan J, Balashov A, Hammer M, Kirli K, Boehm U, Brown CM, Bellve K, Chambers JJ, Cosolo A, Coleman RA, Faklaris O, Fogarty KE, Guilbert T, Hamacher AB, Itano MS, Keeley DP, Kunis S, Lacoste J, Laude A, Ma WY, Marcello M, Montero-Llopis P, Nelson G, Nitschke R, Pimentel JA, Weidtkamp-Peters S, Park PJ, Alver BH, Grunwald D, Strambio-De-Castillia C. Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications. Nat Methods. 2021 12; 18(12):1489-1495. PMID: 34862503.
      Citations:    Fields:    Translation:HumansAnimalsCells
    4. Qu M, Lu P, Bellve K, Lifshitz LM, ZhuGe R. Mode Switch of Ca2 + Oscillation-Mediated Uterine Peristalsis and Associated Embryo Implantation Impairments in Mouse Adenomyosis. Front Physiol. 2021; 12:744745. PMID: 34803733.
      Citations:    
    5. Zhang L, Zhang M, Bellve K, Fogarty KE, Castro MA, Brauchi S, Kobertz WR. Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes. J Gen Physiol. 2020 06 01; 152(6). PMID: 31978216.
      Citations: 1     Fields:    Translation:AnimalsCells
    6. Qu M, Lu P, Bellve K, Fogarty K, Lifshitz L, Shi F, Zhuge R. Smooth muscle cell-specific TMEM16A deletion does not alter Ca2+ signaling, uterine contraction, gestation length, or litter size in mice†. Biol Reprod. 2019 08 01; 101(2):318-327. PMID: 31175367.
      Citations: 3     Fields:    Translation:AnimalsCells
    7. Zheng K, Lu P, Delpapa E, Bellve K, Deng R, Condon JC, Fogarty K, Lifshitz LM, Simas TAM, Shi F, ZhuGe R. Bitter taste receptors as targets for tocolytics in preterm labor therapy. FASEB J. 2017 09; 31(9):4037-4052. PMID: 28559440.
      Citations: 12     Fields:    Translation:HumansAnimals
    8. Zhang L, Bellve K, Fogarty K, Kobertz WR. Fluorescent Visualization of Cellular Proton Fluxes. Cell Chem Biol. 2016 Dec 22; 23(12):1449-1457. PMID: 27916567.
      Citations: 5     Fields:    
    9. Ly S, Navaroli DM, Didiot MC, Cardia J, Pandarinathan L, Alterman JF, Fogarty K, Standley C, Lifshitz LM, Bellve KD, Prot M, Echeverria D, Corvera S, Khvorova A. Visualization of self-delivering hydrophobically modified siRNA cellular internalization. Nucleic Acids Res. 2017 01 09; 45(1):15-25. PMID: 27899655.
      Citations: 37     Fields:    Translation:HumansAnimalsCells
    10. Kubo T, Brown JM, Bellve K, Craige B, Craft JM, Fogarty K, Lechtreck KF, Witman GB. Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. J Cell Sci. 2016 05 15; 129(10):2106-19. PMID: 27068536.
      Citations: 36     Fields:    Translation:AnimalsCells
    11. Wu S, Bellve KD, Fogarty KE, Melikian HE. Ack1 is a dopamine transporter endocytic brake that rescues a trafficking-dysregulated ADHD coding variant. Proc Natl Acad Sci U S A. 2015 Dec 15; 112(50):15480-5. PMID: 26621748.
      Citations: 30     Fields:    Translation:HumansCells
    12. Rojas-Rodriguez R, Lifshitz LM, Bellve KD, Min SY, Pires J, Leung K, Boeras C, Sert A, Draper JT, Corvera S, Moore Simas TA. Human adipose tissue expansion in pregnancy is impaired in gestational diabetes mellitus. Diabetologia. 2015 Sep; 58(9):2106-14. PMID: 26067361.
      Citations: 14     Fields:    Translation:HumansCells
    13. Lefkowitz JJ, DeCrescenzo V, Duan K, Bellve KD, Fogarty KE, Walsh JV, ZhuGe R. Catecholamine exocytosis during low frequency stimulation in mouse adrenal chromaffin cells is primarily asynchronous and controlled by the novel mechanism of Ca2+ syntilla suppression. J Physiol. 2014 Nov 01; 592(21):4639-55. PMID: 25128575.
      Citations: 3     Fields:    Translation:AnimalsCells
    14. Rojas-Rodriguez R, Gealekman O, Kruse ME, Rosenthal B, Rao K, Min S, Bellve KD, Lifshitz LM, Corvera S. Adipose tissue angiogenesis assay. Methods Enzymol. 2014; 537:75-91. PMID: 24480342.
      Citations: 13     Fields:    Translation:HumansAnimalsCells
    15. Guo D, Standley C, Bellve K, Fogarty K, Bao ZZ. Protein kinase C? and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog. Mol Cell Neurosci. 2012 May; 50(1):82-92. PMID: 22521536.
      Citations: 11     Fields:    Translation:AnimalsCells
    16. De Crescenzo V, Fogarty KE, Lefkowitz JJ, Bellve KD, Zvaritch E, MacLennan DH, Walsh JV. Type 1 ryanodine receptor knock-in mutation causing central core disease of skeletal muscle also displays a neuronal phenotype. Proc Natl Acad Sci U S A. 2012 Jan 10; 109(2):610-5. PMID: 22203976.
      Citations: 17     Fields:    Translation:AnimalsCells
    17. Lefkowitz JJ, Fogarty KE, Lifshitz LM, Bellve KD, Tuft RA, ZhuGe R, Walsh JV, De Crescenzo V. Suppression of Ca2+ syntillas increases spontaneous exocytosis in mouse adrenal chromaffin cells. J Gen Physiol. 2009 Oct; 134(4):267-80. PMID: 19786582.
      Citations: 6     Fields:    Translation:AnimalsCells
    18. Li X, Standley C, Sapp E, Valencia A, Qin ZH, Kegel KB, Yoder J, Comer-Tierney LA, Esteves M, Chase K, Alexander J, Masso N, Sobin L, Bellve K, Tuft R, Lifshitz L, Fogarty K, Aronin N, DiFiglia M. Mutant huntingtin impairs vesicle formation from recycling endosomes by interfering with Rab11 activity. Mol Cell Biol. 2009 Nov; 29(22):6106-16. PMID: 19752198.
      Citations: 51     Fields:    Translation:HumansCells
    19. Kolpak AL, Jiang J, Guo D, Standley C, Bellve K, Fogarty K, Bao ZZ. Negative guidance factor-induced macropinocytosis in the growth cone plays a critical role in repulsive axon turning. J Neurosci. 2009 Aug 26; 29(34):10488-98. PMID: 19710302.
      Citations: 33     Fields:    Translation:AnimalsCells
    20. Leonard D, Hayakawa A, Lawe D, Lambright D, Bellve KD, Standley C, Lifshitz LM, Fogarty KE, Corvera S. Sorting of EGF and transferrin at the plasma membrane and by cargo-specific signaling to EEA1-enriched endosomes. J Cell Sci. 2008 Oct 15; 121(Pt 20):3445-58. PMID: 18827013.
      Citations: 64     Fields:    Translation:AnimalsCells
    21. Huang S, Lifshitz LM, Jones C, Bellve KD, Standley C, Fonseca S, Corvera S, Fogarty KE, Czech MP. Insulin stimulates membrane fusion and GLUT4 accumulation in clathrin coats on adipocyte plasma membranes. Mol Cell Biol. 2007 May; 27(9):3456-69. PMID: 17339344.
      Citations: 43     Fields:    Translation:AnimalsCells
    22. Hayakawa A, Leonard D, Murphy S, Hayes S, Soto M, Fogarty K, Standley C, Bellve K, Lambright D, Mello C, Corvera S. The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis. Proc Natl Acad Sci U S A. 2006 Aug 08; 103(32):11928-33. PMID: 16873553.
      Citations: 28     Fields:    Translation:HumansAnimalsCells
    23. Bellve KD, Leonard D, Standley C, Lifshitz LM, Tuft RA, Hayakawa A, Corvera S, Fogarty KE. Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells. J Biol Chem. 2006 Jun 09; 281(23):16139-46. PMID: 16537543.
      Citations: 30     Fields:    Translation:AnimalsCells
    24. Scriven DR, Klimek A, Asghari P, Bellve K, Moore ED. Caveolin-3 is adjacent to a group of extradyadic ryanodine receptors. Biophys J. 2005 Sep; 89(3):1893-901. PMID: 15980179.
      Citations: 41     Fields:    Translation:AnimalsCells
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