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Jose R Lemos PhD

TitleProfessor Emeritus
InstitutionUniversity of Massachusetts Medical School
DepartmentMicrobiology and Physiological Systems
AddressUniversity of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Phone508-856-8567
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionUMMS - School of Medicine
    DepartmentMicrobiology and Physiological Systems

    InstitutionUMMS - School of Medicine
    DepartmentNeuroNexus Institute

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentNeuroscience


    Collapse Biography 
    Collapse education and training
    Occidental College, Los Angeles, CA, United StatesBAPsychology
    Wesleyan University, Middleton, CT, United StatesPH DBiology & Psychology
    Collapse awards and honors
    2006Outstanding Mentor Award, BSCP
    2015Dean’s Award for Outstanding Faculty Contribution to Graduate Education , UMMS

    Collapse Overview 
    Collapse overview

    Academic Background

    1970, B.A., Occidental College
    1979, Ph.D., Wesleyan University

    Stimulus-secretion Coupling at Nerve Terminals

    Photo: José R. Lemos

    The small size and inaccessibility of most nerve terminals has not, until recently, allowed direct measurement of their individual electrophysiological properties. Thus, the molecular details of how ionic currents control the release of neuroactive substances remain undetermined. We can isolate nerve terminals from the rat posterior pituitary (PP) which respond to depolarization by releasing, in a calcium (Ca2+) dependent manner, peptide neurohormones via exocytosis. A combination of patch-clamp, biochemical, imaging, and molecular biological techniques are being used to try to understand how electrical patterns of activity, drugs (such as alcohol), endogenous transmitters (such as ATP), and toxins regulate Ca2+ entry and subsequent transmitter release at these nerve terminals.

    Ca2+ channels
    We have characterized "L"- and "N"-like Ca2+ channels in these PP terminals, which are quite different from their counterparts on the cell body. Dihydropyridine-Ca2+ channel agonists and antagonists, which modulate the L-type activity, have no effect on the "Nt"-type channel which is most susceptible to block by omega (w)-conotoxin GVIA. Recently we have been able to prevent dialysis of the cytoplasm of the terminal by utilizing amphotericin B to perforate nerve terminals through the patch in the pipette. This technical improvement has enabled us to perform hitherto impossible studies on the Ca2+ channels in the "intact" nerve terminals, including the testing of synthetic and mutated toxins. This has led to the positive identification of a third or "Q" type of Ca2+ channel in AVP-releasing terminals. Funnel-Web spider toxins and w-conotoxin MVIIC block this Ca2+-current component. Most recently, we have identified a fourth or "R" type of Ca2+ channel in OT-releasing terminals.

    Localization
    Fluorescently-tagged specific blockers of the L and N type Ca2+ channels allowed us to determine their distribution in these terminals (see Fig. 1) using a laser confocal microscope. These preliminary results indicate that it should be feasible not only to localize different types of Ca2+ channels in individual PP terminals, but to even do so in relation to possible release sites.

    Figure 1

    Figure 1. Localization of Ca2+ channels. The DM-bodipy labelled dihydropyridine (specific for L-type channel) appeared to be uniformly distributed (A, right panel) in all types of terminals (A, left panel: using transmitted light), and even in endocrine cells. In contrast, the fluorescein-labeled w-Cgtx GVIA probe (specific for N-type) was mainly found in discrete hot spots (B: 4X zoom of two terminals) and only on neurohypophysial terminals. It was also possible, using reflected light scattering (i.e., the "Tyndal effect") to observe the NSG within individual NH terminals (C: 4X zoom of two terminals). These disappear rapidly in response to puffs of high (100 mM) K+, perhaps indicating exocytotic release at particular sites.

    Electrical activity
    We have studied several other currents in order to understand how bursting electrical activity is generated and regulated. We have characterized a novel Ca2+ activated K+ channel, specifically blocked by the anti-hypertension drug, tetrandrine, in the PP terminals that could play a role in terminating bursts. This Ca2+-activated K+ channel is activated by intracellular applications of Mg-ATP, apparently via an endogenous kinase. We are currently attempting to identify this kinase and the physiological effector for this "up-regulation". We are also attempting to determine why different bursting patterns of activity are necessary to release vasopressin vs. oxytocin from these nerve terminals.

    ATP feedback
    Since substances, such as ATP, are co-released from neurosecretory granules (NSG), we investigated whether ATP could actually affect peptide secretion. ATP exhibited a bi-phasic effect, initially potentiating via a P2x2 receptor and then inhibiting via an A1, receptor vasopressin release. Furthermore, both the Ca2+-activated K+ and the N-type Ca2+ channels are modulated by ATP co-released with the peptides. The endogenous effects of both ATP and its metabolite, Adenosine, could help explain the differential burst effects on release of the neuropeptides.

    Ethanol effects
    Ingestion of ethanol (EtOH) is known to result in a reduction of plasma arginine-vasopressin (AVP) levels in mammals. Release of AVP from nerve terminals isolated from the rat neurohypophysis was very sensitive to EtOH. Patch clamping of these terminals indicated that both inactivating and long-lasting calcium currents were reduced in EtOH, but that the long-lasting single channel currents were most sensitive. EtOH-induced decreases in plasma AVP levels can be explained by EtOH's inhibition of Ca2+- and potentiation ofCa2+-activated K+-currents in the nerve terminals.

    We (in collaboration with S. Treistman) have shown for the first time that EtOH can directly affect specific ion channels involved in a physiological response.

    Opioid effects
    Opioids interact with receptors on neurons, leading to a variety of effects, e.g., analgesia, euphoria, and diuresis. It is not known, however, whether these effects are at somata and/or synapses in the central nervous system (CNS). The electrical and secretory activities of the hypothalamo-neurohypophysial system (HNS) are affected by both exogenous and endogenous opioids. Furthermore, the HNS develops tolerance and dependence to morphine during chronic administration suggesting that this CNS system is a good model for studying the physiological mechanisms underlying these phenomena. For example, µ-opioid specific effects on oxytocin release can be explained by its targeting of R-type Ca-channels found only on oxytocin terminals.

    Calcium sparks in nerve terminals
    As a model for exocytosis we have been studying, in collaboration with John Walsh, the role of intracellular Calcium in nerve terminals. We have now shown that Ca-sparks or "syntillas" exist in neurohypophysial terminals of the mouse. Most recently, we have been studying the activation of these ryanodine Ca2+ channels by voltage and the identity of the Ca-stores in nerve terminals.

    Mechanism of exocytosis

    We have been attempting to reconstitute channel-forming proteins from the NSG of rat and bovine PP terminals in order to study their properties. We have observed both an anion and a Ca2+ activated cation channel in these membranes. We have hypothesized that the NSG channels may play a direct role in the mechanism underlying exocytosis (see Figure 2). Blockers of this channel also block release of peptides from the permeabilized PP terminals. Most importantly, both the Ca2+-activated NSG channel and Ca2+-dependent release are inhibited by an antibody directed against the putative Ca2+-binding site of synaptophysin, an integral NSG membrane protein. We are now in the process of utilizing a molecular genetic approach to try to determine if and how this vesicular channel is involved in exocytosis. Most recently, we have discovered that the NSG channel is actually the ryanodine receptor and thus could mediate “syntillas” in nerve terminals.

    Figure 2

    Figure 2. Model of depolarization-secretion coupling: (1) A complex of proteins serve to "dock" the NSG. This complex possibly involves VAMP (synaptobrevin) and synaptotagmin (p65) interacting via SNAP-25 with syntaxin. (2) Synaptotagmin is liberated by the binding ofa-SNAP (a) and then by NSF. According to our data, Calcium (Ca) enters through, at least, three types of Ca channels and elevates its intracellular levels [Ca]. (3) We hypothesize that Ca could then bind to synaptotagmin and relieve its inhibition (-) of the NSG channel that we have shown to likely be synaptophysin. (4) Synaptophysin can interact with certain plasma membrane proteins such as physophilin, and the opening of the apposed channels could then form a gap junction-like "fusion pore" across the two membranes. (5) When the fusion pore is open, extracellular cations would move into the NSG down their concentration and/or electrical gradients. (6) The subsequent osmotic increase would force water to enter the NSG and cause them to swell. (8) Entry of ions, would also disrupt the matrix inside the vesicle and lead to subsequent expulsion of the contents of the NSG, perhaps even through the fusion pore itself.



    Collapse Rotation Projects

    Potential Rotation Projects

    1. Drugs of abuse: Loose patch clamping of organ cultures from whole brain in order to determine what ion channels are affected by opioids and other drugs of abuse. Determining specific calcium channel subtypes and localization in relationship to release sites using confocal imaging techniques.

    2. Molecular mechanisms of synaptic transmission: Bi-layers/Tip dipping: recording single channels reconstituted from posterior pituitary nerve terminals using patch clamp techniques. Reconstitute Ca2+-dependent exocytosis in vitro by a combination of amperometric and molecular biological techniques.

    3. Endogenous Feedback regulation of neuropeptide release: Determine different synaptic neurotransmitter (ATP) and opioid receptor subtypes and localization on oxytocin vs. vasopressin releasing nerve terminals using immunohistochemistry and deconvolution techniques.




    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Marrero HG, Treistman SN, Lemos JR. Ethanol Effect on BK Channels is Modulated by Magnesium. Alcohol Clin Exp Res. 2015 Sep; 39(9):1671-9. PMID: 26331878.
      View in: PubMed
    2. McNally JM, Custer EE, Ortiz-Miranda S, Woodbury DJ, Kraner SD, Salzberg BM, Lemos JR. Functional ryanodine receptors in the membranes of neurohypophysial secretory granules. J Gen Physiol. 2014 Jun; 143(6):693-702. PMID: 24863930.
      View in: PubMed
    3. Velázquez-Marrero C, Ortiz-Miranda S, Marrero HG, Custer EE, Treistman SN, Lemos JR. µ-Opioid inhibition of Ca2+ currents and secretion in isolated terminals of the neurohypophysis occurs via ryanodine-sensitive Ca2+ stores. J Neurosci. 2014 Mar 5; 34(10):3733-42. PMID: 24599471.
      View in: PubMed
    4. Cuadra AE, Custer EE, Bosworth EL, Lemos JR. P2X7 receptors in neurohypophysial terminals: evidence for their role in arginine-vasopressin secretion. J Cell Physiol. 2014 Mar; 229(3):333-42. PMID: 24037803.
      View in: PubMed
    5. Pietrzykowski AZ, Ortiz-Miranda S, Knott TK, Custer E, Puig S, Lemos JR, Treistman SN. Molecular tolerance of voltage-gated calcium channels is evident after short exposures to alcohol in vasopressin-releasing nerve terminals. Alcohol Clin Exp Res. 2013 Jun; 37(6):933-40. PMID: 23421938.
      View in: PubMed
    6. Poderoso C, Duarte A, Cooke M, Orlando U, Gottifredi V, Solano AR, Lemos JR, Podestá EJ. The spatial and temporal regulation of the hormonal signal. Role of mitochondria in the formation of a protein complex required for the activation of cholesterol transport and steroids synthesis. Mol Cell Endocrinol. 2013 May 22; 371(1-2):26-33. PMID: 23357790.
      View in: PubMed
    7. Lemos JR, Ortiz-Miranda SI, Cuadra AE, Velázquez-Marrero C, Custer EE, Dad T, Dayanithi G. Modulation/physiology of calcium channel sub-types in neurosecretory terminals. Cell Calcium. 2012 Mar-Apr; 51(3-4):284-92. PMID: 22341671.
      View in: PubMed
    8. Velázquez-Marrero CM, Marrero HG, Lemos JR. Voltage-dependent kappa-opioid modulation of action potential waveform-elicited calcium currents in neurohypophysial terminals. J Cell Physiol. 2010 Oct; 225(1):223-32. PMID: 20506396.
      View in: PubMed
    9. Ortiz-Miranda SI, Dayanithi G, Velázquez-Marrero C, Custer EE, Treistman SN, Lemos JR. Differential modulation of N-type calcium channels by micro-opioid receptors in oxytocinergic versus vasopressinergic neurohypophysial terminals. J Cell Physiol. 2010 Oct; 225(1):276-88. PMID: 20509142.
      View in: PubMed
    10. McNally JM, De Crescenzo V, Fogarty KE, Walsh JV, Lemos JR. Individual calcium syntillas do not trigger spontaneous exocytosis from nerve terminals of the neurohypophysis. J Neurosci. 2009 Nov 11; 29(45):14120-6. PMID: 19906960.
      View in: PubMed
    11. Knott TK, Marrero HG, Custer EE, Lemos JR. Endogenous ATP potentiates only vasopressin secretion from neurohypophysial terminals. J Cell Physiol. 2008 Oct; 217(1):155-61. PMID: 18481265.
      View in: PubMed
    12. Custer EE, Ortiz-Miranda S, Knott TK, Rawson R, Elvey C, Lee RH, Lemos JR. Identification of the neuropeptide content of individual rat neurohypophysial terminals. J Neurosci Methods. 2007 Jul 30; 163(2):226-34. PMID: 17452053.
      View in: PubMed
    13. ZhuGe R, DeCrescenzo V, Sorrentino V, Lai FA, Tuft RA, Lifshitz LM, Lemos JR, Smith C, Fogarty KE, Walsh JV. Syntillas release Ca2+ at a site different from the microdomain where exocytosis occurs in mouse chromaffin cells. Biophys J. 2006 Mar 15; 90(6):2027-37. PMID: 16387759.
      View in: PubMed
    14. Pietrzykowski AZ, Martin GE, Puig SI, Knott TK, Lemos JR, Treistman SN. Alcohol tolerance in large-conductance, calcium-activated potassium channels of CNS terminals is intrinsic and includes two components: decreased ethanol potentiation and decreased channel density. J Neurosci. 2004 Sep 22; 24(38):8322-32. PMID: 15385615.
      View in: PubMed
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