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Christopher M Sassetti PhD

TitleProfessor
InstitutionUniversity of Massachusetts Medical School
DepartmentMicrobiology and Physiological Systems
AddressUniversity of Massachusetts Medical School
55 Lake Avenue North, AS8-2051
Worcester MA 01655
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentMicrobiology and Physiological Systems

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentMolecular Genetics and Microbiology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentTranslational Science

    InstitutionUMMS - Programs, Centers and Institutes
    DepartmentBioinformatics and Integrative Biology


    Collapse Biography 
    Collapse education and training
    Santa Clara University, Santa Clara, CA, United StatesBSBiology
    University of California, San Francisco, San Francisco, CA, United StatesPHDImmunology

    Collapse Overview 
    Collapse overview

    Academic Background:

    Ph.D. University of California, San Francisco, CA. 2000
    Damon Runyon Foundation Scholar (www.damonrunyon.org)

    Other:

    Howard Hughes Medical Institute Investigator (www.hhmi.org)

    Pathogenesis of tuberculosis

    Christopher Sassetti, Ph.D.

    Mycobacterium tuberculosis is often called the world's most successful pathogen. It is estimated that one third of the human population has been exposed to this organism, and tuberculosis (TB) kills millions every year. Unlike many other bacterial pathogens that cause acute disease and replicate only in a specific host niche, M. tuberculosis can maintain a chronic infection by adapting to many distinctly different host microenvironments. Our lab is focused on defining the survival strategies used by this pathogen in each of these environments.

    To this end, we have developed a variety of new methodologies, which take advantage of both classical genetic tools and genome sequence information. Using these methods, we have identified hundreds of mycobacterial genes that are specifically required for the bacterium to survive under a variety of conditions including acute and chronic infection models. Current work is focused on the characterizing the functional roles played by these virulence systems.

    Specific projects include:

    1)Understanding how nutrients are acquired in vivo. M. tuberculosis resides largely within a membrane bound compartment during infection, and it remains unclear how the bacterium acquires nutrients in this apparently isolated niche. We have identified a series of lipid and carbohydrate import systems that are critical for growth during specific phases of disease. One of these functions as a sterol uptake system, which has lead to the discovery that host cholesterol is an essential carbon source during chronic infection (see figure). We are currently focused on defining the mechanisms by which the bacterium extracts and degrades nutrients, such as cholesterol, from the host cell and understanding why the nutritional habits of the bacterium change so dramatically as disease progresses.

    Cholesterol

    RFP-labeledM. tuberculosis resides in
    cholesterol rich regions of the cell (green).

    2)Understanding the unique physiology of the mycobacterial cell wall. The cell envelope of M. tuberculosis forms a unique barrier to environmental insults and is essential for the viability of the pathogen. Using a combination of high-throughput genetic, biochemical and structural approaches, we are investigating how phosphosignaling cascades regulate the synthesis of this complex structure.

    Cell Wall Systhesis

    Cell wall synthesis (green) is dysregulated in mycobacterial mutants compared to wild type cells (red).

    3)Metabolic regulation of cell growth and antibiotic sensitivity. The immune system of the host exerts a variety of stresses on the bacterium, which cause it to stop replicating and become refractory to antibiotic treatment. Using a combination of genetic and metabolomic approaches, we are defining the mechanisms by which M. tuberculosis senses these stresses and responds by reducing its growth and metabolic rate.

    To see information on the Sassetti Lab .

    For more information, visit hisHoward Hughes Medical Institute web page.



    Collapse Rotation Projects

    Rotation Projects

    We have identified a number of bacterial pathways that are required for bacterial viability or virulence. We are particularly interested in:

    1. Phosphosignaling systems that regulate cell wall synthesis
    2. Metabolic regulatory mechanisms that trigger the “dormancy” response observed during infection.

    A variety of genetic, biochemical, and informatic methods will be used to define the functions of these genes and the role played by each during infection.




    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Bellerose MM, Baek SH, Huang CC, Moss CE, Koh EI, Proulx MK, Smith CM, Baker RE, Lee JS, Eum S, Shin SJ, Cho SN, Murray M, Sassetti CM. Common Variants in the Glycerol Kinase Gene Reduce Tuberculosis Drug Efficacy. MBio. 2019 Jul 30; 10(4). PMID: 31363023.
      View in: PubMed
    2. Johnson EO, LaVerriere E, Office E, Stanley M, Meyer E, Kawate T, Gomez JE, Audette RE, Bandyopadhyay N, Betancourt N, Delano K, Da Silva I, Davis J, Gallo C, Gardner M, Golas AJ, Guinn KM, Kennedy S, Korn R, McConnell JA, Moss CE, Murphy KC, Nietupski RM, Papavinasasundaram KG, Pinkham JT, Pino PA, Proulx MK, Ruecker N, Song N, Thompson M, Trujillo C, Wakabayashi S, Wallach JB, Watson C, Ioerger TR, Lander ES, Hubbard BK, Serrano-Wu MH, Ehrt S, Fitzgerald M, Rubin EJ, Sassetti CM, Schnappinger D, Hung DT. Large-scale chemical-genetics yields new M. tuberculosis inhibitor classes. Nature. 2019 Jun 19. PMID: 31217586.
      View in: PubMed
    3. Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM. ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes. MBio. 2018 Dec 11; 9(6). PMID: 30538179.
      View in: PubMed
    4. Chen Y, Sharma S, Assis PA, Jiang Z, Elling R, Olive AJ, Hang S, Bernier J, Huh JR, Sassetti CM, Knipe DM, Gazzinelli RT, Fitzgerald KA. CNBP controls IL-12 gene transcription and Th1 immunity. J Exp Med. 2018 Nov 15. PMID: 30442645.
      View in: PubMed
    5. Daniel-Wayman S, Abate G, Barber DL, Bermudez LE, Coler RN, Cynamon MH, Daley CL, Davidson RM, Dick T, Floto RA, Henkle E, Holland SM, Jackson M, Lee RE, Nuermberger EL, Olivier KN, Ordway DJ, Prevots DR, Sacchettini JC, Salfinger M, Sassetti CM, Sizemore CF, Winthrop KL, Zelazny AM. Advancing Translational Science for Pulmonary NTM Infections: A Roadmap for Research. Am J Respir Crit Care Med. 2018 Nov 14. PMID: 30428263.
      View in: PubMed
    6. Rittershaus ESC, Baek SH, Krieger IV, Nelson SJ, Cheng YS, Nambi S, Baker RE, Leszyk JD, Shaffer SA, Sacchettini JC, Sassetti CM. A Lysine Acetyltransferase Contributes to the Metabolic Adaptation to Hypoxia in Mycobacterium tuberculosis. Cell Chem Biol. 2018 Oct 04. PMID: 30318462.
      View in: PubMed
    7. Olive AJ, Sassetti CM. Tolerating the Unwelcome Guest; How the Host Withstands Persistent Mycobacterium tuberculosis. Front Immunol. 2018; 9:2094. PMID: 30258448.
      View in: PubMed
    8. Smith CM, Sassetti CM. Modeling Diversity: Do Homogeneous Laboratory Strains Limit Discovery? Trends Microbiol. 2018 Aug 27. PMID: 30166218.
      View in: PubMed
    9. Olive AJ, Smith CM, Kiritsy MC, Sassetti CM. The Phagocyte Oxidase Controls Tolerance to Mycobacterium tuberculosis Infection. J Immunol. 2018 Jul 30. PMID: 30061198.
      View in: PubMed
    10. Köster S, Klevorn T, Papavinasasundaram K, Sassetti CM, Portal-Celhay C, Philips JA. Consequence of enhanced LC3-trafficking for a live, attenuated M. tuberculosis vaccine. Vaccine. 2018 02 08; 36(7):939-944. PMID: 29343411.
      View in: PubMed
    11. Logsdon MM, Ho PY, Papavinasasundaram K, Richardson K, Cokol M, Sassetti CM, Amir A, Aldridge BB. A Parallel Adder Coordinates Mycobacterial Cell-Cycle Progression and Cell-Size Homeostasis in the Context of Asymmetric Growth and Organization. Curr Biol. 2017 Nov 06; 27(21):3367-3374.e7. PMID: 29107550.
      View in: PubMed
    12. Rothchild AC, Stowell B, Goyal G, Nunes-Alves C, Yang Q, Papavinasasundaram K, Sassetti CM, Dranoff G, Chen X, Lee J, Behar SM. Role of Granulocyte-Macrophage Colony-Stimulating Factor Production by T Cells during Mycobacterium tuberculosis Infection. MBio. 2017 10 24; 8(5). PMID: 29066547.
      View in: PubMed
    13. Köster S, Upadhyay S, Chandra P, Papavinasasundaram K, Yang G, Hassan A, Grigsby SJ, Mittal E, Park HS, Jones V, Hsu FF, Jackson M, Sassetti CM, Philips JA. Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA. Proc Natl Acad Sci U S A. 2017 10 10; 114(41):E8711-E8720. PMID: 28973896.
      View in: PubMed
    14. García-Fernández J, Papavinasasundaram K, Galán B, Sassetti CM, García JL. Molecular and functional analysis of the mce4 operon in Mycobacterium smegmatis. Environ Microbiol. 2017 Sep; 19(9):3689-3699. PMID: 28752922.
      View in: PubMed
    15. Mishra BB, Lovewell RR, Olive AJ, Zhang G, Wang W, Eugenin E, Smith CM, Phuah JY, Long JE, Dubuke ML, Palace SG, Goguen JD, Baker RE, Nambi S, Mishra R, Booty MG, Baer CE, Shaffer SA, Dartois V, McCormick BA, Chen X, Sassetti CM. Nitric oxide prevents a pathogen-permissive granulocytic inflammation during tuberculosis. Nat Microbiol. 2017 May 15; 2:17072. PMID: 28504669.
      View in: PubMed
    16. García-Fernández J, Papavinasasundaram K, Galán B, Sassetti CM, García JL. Unravelling the pleiotropic role of the MceG ATPase in Mycobacterium smegmatis. Environ Microbiol. 2017 Jul; 19(7):2564-2576. PMID: 28447386.
      View in: PubMed
    17. DeJesus MA, Nambi S, Smith CM, Baker RE, Sassetti CM, Ioerger TR. Statistical analysis of genetic interactions in Tn-Seq data. Nucleic Acids Res. 2017 Feb 22. PMID: 28334803.
      View in: PubMed
    18. Rock JM, Hopkins FF, Chavez A, Diallo M, Chase MR, Gerrick ER, Pritchard JR, Church GM, Rubin EJ, Sassetti CM, Schnappinger D, Fortune SM. Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Nat Microbiol. 2017 Feb 06; 2:16274. PMID: 28165460.
      View in: PubMed
    19. DeJesus MA, Gerrick ER, Xu W, Park SW, Long JE, Boutte CC, Rubin EJ, Schnappinger D, Ehrt S, Fortune SM, Sassetti CM, Ioerger TR. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. MBio. 2017 Jan 17; 8(1). PMID: 28096490.
      View in: PubMed
    20. Smith CM, Proulx MK, Olive AJ, Laddy D, Mishra BB, Moss C, Gutierrez NM, Bellerose MM, Barreira-Silva P, Phuah JY, Baker RE, Behar SM, Kornfeld H, Evans TG, Beamer G, Sassetti CM. Tuberculosis Susceptibility and Vaccine Protection Are Independently Controlled by Host Genotype. MBio. 2016 Sep 20; 7(5). PMID: 27651361.
      View in: PubMed
    21. Prigozhin DM, Papavinasasundaram KG, Baer CE, Murphy KC, Moskaleva A, Chen TY, Alber T, Sassetti CM. Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site. J Biol Chem. 2016 Oct 28; 291(44):22961-22969. PMID: 27601474.
      View in: PubMed
    22. Boutte CC, Baer CE, Papavinasasundaram K, Liu W, Chase MR, Meniche X, Fortune SM, Sassetti CM, Ioerger TR, Rubin EJ. A cytoplasmic peptidoglycan amidase homologue controls mycobacterial cell wall synthesis. Elife. 2016 Jun 15; 5. PMID: 27304077.
      View in: PubMed
    23. Cai Y, Yang Q, Liao M, Wang H, Zhang C, Nambi S, Wang W, Zhang M, Wu J, Deng G, Deng Q, Liu H, Zhou B, Jin Q, Feng CG, Sassetti CM, Wang F, Chen X. xCT increases tuberculosis susceptibility by regulating antimicrobial function and inflammation. Oncotarget. 2016 May 24; 7(21):31001-13. PMID: 27129162.
      View in: PubMed
    24. Hayashi JM, Luo CY, Mayfield JA, Hsu T, Fukuda T, Walfield AL, Giffen SR, Leszyk JD, Baer CE, Bennion OT, Madduri A, Shaffer SA, Aldridge BB, Sassetti CM, Sandler SJ, Kinoshita T, Moody DB, Morita YS. Spatially distinct and metabolically active membrane domain in mycobacteria. Proc Natl Acad Sci U S A. 2016 May 10; 113(19):5400-5. PMID: 27114527.
      View in: PubMed
    25. Patel SJ, Lewis BE, Long JE, Nambi S, Sassetti CM, Stemmler TL, Argüello JM. Fine-tuning of Substrate Affinity Leads to Alternative Roles of Mycobacterium tuberculosis Fe2+-ATPases. J Biol Chem. 2016 May 27; 291(22):11529-39. PMID: 27022029.
      View in: PubMed
    26. Olive AJ, Sassetti CM. Metabolic crosstalk between host and pathogen: sensing, adapting and competing. Nat Rev Microbiol. 2016 Apr; 14(4):221-34. PMID: 26949049.
      View in: PubMed
    27. Lovewell RR, Sassetti CM, VanderVen BC. Chewing the fat: lipid metabolism and homeostasis during M. tuberculosis infection. Curr Opin Microbiol. 2016 Feb; 29:30-6. PMID: 26544033.
      View in: PubMed
    28. DeJesus MA, Ambadipudi C, Baker R, Sassetti C, Ioerger TR. TRANSIT--A Software Tool for Himar1 TnSeq Analysis. PLoS Comput Biol. 2015 Oct; 11(10):e1004401. PMID: 26447887.
      View in: PubMed
    29. Kieser KJ, Baranowski C, Chao MC, Long JE, Sassetti CM, Waldor MK, Sacchettini JC, Ioerger TR, Rubin EJ. Peptidoglycan synthesis in Mycobacterium tuberculosis is organized into networks with varying drug susceptibility. Proc Natl Acad Sci U S A. 2015 Oct 20; 112(42):13087-92. PMID: 26438867.
      View in: PubMed
    30. Kieser KJ, Boutte CC, Kester JC, Baer CE, Barczak AK, Meniche X, Chao MC, Rego EH, Sassetti CM, Fortune SM, Rubin EJ. Phosphorylation of the Peptidoglycan Synthase PonA1 Governs the Rate of Polar Elongation in Mycobacteria. PLoS Pathog. 2015 Jun; 11(6):e1005010. PMID: 26114871.
      View in: PubMed
    31. Nambi S, Long JE, Mishra BB, Baker R, Murphy KC, Olive AJ, Nguyen HP, Shaffer SA, Sassetti CM. The Oxidative Stress Network of Mycobacterium tuberculosis Reveals Coordination between Radical Detoxification Systems. Cell Host Microbe. 2015 Jun 10; 17(6):829-37. PMID: 26067605.
      View in: PubMed
    32. Feltcher ME, Gunawardena HP, Zulauf KE, Malik S, Griffin JE, Sassetti CM, Chen X, Braunstein M. Label-free Quantitative Proteomics Reveals a Role for the Mycobacterium tuberculosis SecA2 Pathway in Exporting Solute Binding Proteins and Mce Transporters to the Cell Wall. Mol Cell Proteomics. 2015 Jun; 14(6):1501-16. PMID: 25813378.
      View in: PubMed
    33. Baer CE, Rubin EJ, Sassetti CM. New insights into TB physiology suggest untapped therapeutic opportunities. Immunol Rev. 2015 Mar; 264(1):327-43. PMID: 25703570.
      View in: PubMed
    34. Long JE, DeJesus M, Ward D, Baker RE, Ioerger T, Sassetti CM. Identifying essential genes in Mycobacterium tuberculosis by global phenotypic profiling. Methods Mol Biol. 2015; 1279:79-95. PMID: 25636614.
      View in: PubMed
    35. Murphy KC, Papavinasasundaram K, Sassetti CM. Mycobacterial recombineering. Methods Mol Biol. 2015; 1285:177-99. PMID: 25779316.
      View in: PubMed
    36. Olive AJ, Sassetti CM. New TB treatments hiding in plain sight. EMBO Mol Med. 2015 Feb; 7(2):125-6. PMID: 25535253.
      View in: PubMed
    37. Zhang G, Zhou B, Li S, Yue J, Yang H, Wen Y, Zhan S, Wang W, Liao M, Zhang M, Zeng G, Feng CG, Sassetti CM, Chen X. Allele-specific induction of IL-1ß expression by C/EBPß and PU.1 contributes to increased tuberculosis susceptibility. PLoS Pathog. 2014 Oct; 10(10):e1004426. PMID: 25329476.
      View in: PubMed
    38. Trauner A, Sassetti CM, Rubin EJ. Genetic Strategies for Identifying New Drug Targets. Microbiol Spectr. 2014 Aug; 2(4):MGM2-0030-2013. PMID: 26104208.
      View in: PubMed
    39. Baer CE, Iavarone AT, Alber T, Sassetti CM. Biochemical and spatial coincidence in the provisional Ser/Thr protein kinase interaction network of Mycobacterium tuberculosis. J Biol Chem. 2014 Jul 25; 289(30):20422-33. PMID: 24928517.
      View in: PubMed
    40. Meniche X, Otten R, Siegrist MS, Baer CE, Murphy KC, Bertozzi CR, Sassetti CM. Subpolar addition of new cell wall is directed by DivIVA in mycobacteria. Proc Natl Acad Sci U S A. 2014 Aug 5; 111(31):E3243-51. PMID: 25049412.
      View in: PubMed
    41. Behar SM, Sassetti CM. Immunology: Fixing the odds against tuberculosis. Nature. 2014 Jul 3; 511(7507):39-40. PMID: 24990740.
      View in: PubMed
    42. Raimunda D, Long JE, Padilla-Benavides T, Sassetti CM, Argüello JM. Differential roles for the Co(2+) /Ni(2+) transporting ATPases, CtpD and CtpJ, in Mycobacterium tuberculosis virulence. Mol Microbiol. 2014 Jan; 91(1):185-97. PMID: 24255990.
      View in: PubMed
    43. Ioerger TR, O'Malley T, Liao R, Guinn KM, Hickey MJ, Mohaideen N, Murphy KC, Boshoff HI, Mizrahi V, Rubin EJ, Sassetti CM, Barry CE, Sherman DR, Parish T, Sacchettini JC. Identification of new drug targets and resistance mechanisms in Mycobacterium tuberculosis. PLoS One. 2013; 8(9):e75245. PMID: 24086479.
      View in: PubMed
    44. Shell SS, Prestwich EG, Baek SH, Shah RR, Sassetti CM, Dedon PC, Fortune SM. DNA methylation impacts gene expression and ensures hypoxic survival of Mycobacterium tuberculosis. PLoS Pathog. 2013; 9(7):e1003419. PMID: 23853579.
      View in: PubMed
    45. Rittershaus ES, Baek SH, Sassetti CM. The normalcy of dormancy: common themes in microbial quiescence. Cell Host Microbe. 2013 Jun 12; 13(6):643-51. PMID: 23768489.
      View in: PubMed
    46. O'Sullivan BP, Sassetti CM. Infection control in cystic fibrosis: share and share alike. Lancet. 2013 May 4; 381(9877):1517-9. PMID: 23541536.
      View in: PubMed
    47. Padilla-Benavides T, Long JE, Raimunda D, Sassetti CM, Argüello JM. A novel P(1B)-type Mn2+-transporting ATPase is required for secreted protein metallation in mycobacteria. J Biol Chem. 2013 Apr 19; 288(16):11334-47. PMID: 23482562.
      View in: PubMed
    48. DeJesus MA, Zhang YJ, Sassetti CM, Rubin EJ, Sacchettini JC, Ioerger TR. Bayesian analysis of gene essentiality based on sequencing of transposon insertion libraries. Bioinformatics. 2013 Mar 15; 29(6):695-703. PMID: 23361328.
      View in: PubMed
    49. Mishra BB, Rathinam VA, Martens GW, Martinot AJ, Kornfeld H, Fitzgerald KA, Sassetti CM. Nitric oxide controls the immunopathology of tuberculosis by inhibiting NLRP3 inflammasome-dependent processing of IL-1ß. Nat Immunol. 2013 Jan; 14(1):52-60. PMID: 23160153.
      View in: PubMed
    50. Zhang YJ, Ioerger TR, Huttenhower C, Long JE, Sassetti CM, Sacchettini JC, Rubin EJ. Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. PLoS Pathog. 2012 Sep; 8(9):e1002946. PMID: 23028335.
      View in: PubMed
    51. Lee HJ, Lang PT, Fortune SM, Sassetti CM, Alber T. Cyclic AMP regulation of protein lysine acetylation in Mycobacterium tuberculosis. Nat Struct Mol Biol. 2012 Aug; 19(8):811-8. PMID: 22773105.
      View in: PubMed
    52. Raimunda D, Long JE, Sassetti CM, Argüello JM. Role in metal homeostasis of CtpD, a Co²? transporting P(1B4)-ATPase of Mycobacterium smegmatis. Mol Microbiol. 2012 Jun; 84(6):1139-49. PMID: 22591178.
      View in: PubMed
    53. Griffin JE, Pandey AK, Gilmore SA, Mizrahi V, McKinney JD, Bertozzi CR, Sassetti CM. Cholesterol catabolism by Mycobacterium tuberculosis requires transcriptional and metabolic adaptations. Chem Biol. 2012 Feb 24; 19(2):218-27. PMID: 22365605.
      View in: PubMed
    54. Gee CL, Papavinasasundaram KG, Blair SR, Baer CE, Falick AM, King DS, Griffin JE, Venghatakrishnan H, Zukauskas A, Wei JR, Dhiman RK, Crick DC, Rubin EJ, Sassetti CM, Alber T. A phosphorylated pseudokinase complex controls cell wall synthesis in mycobacteria. Sci Signal. 2012 Jan 24; 5(208):ra7. PMID: 22275220.
      View in: PubMed
    55. Griffin JE, Gawronski JD, Dejesus MA, Ioerger TR, Akerley BJ, Sassetti CM. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. PLoS Pathog. 2011 Sep; 7(9):e1002251. PMID: 21980284.
      View in: PubMed
    56. Baek SH, Li AH, Sassetti CM. Metabolic regulation of mycobacterial growth and antibiotic sensitivity. PLoS Biol. 2011 May; 9(5):e1001065. PMID: 21629732.
      View in: PubMed
    57. Lee J, Repasy T, Papavinasasundaram K, Sassetti C, Kornfeld H. Mycobacterium tuberculosis induces an atypical cell death mode to escape from infected macrophages. PLoS One. 2011 Mar 31; 6(3):e18367. PMID: 21483832.
      View in: PubMed
    58. Wei JR, Krishnamoorthy V, Murphy K, Kim JH, Schnappinger D, Alber T, Sassetti CM, Rhee KY, Rubin EJ. Depletion of antibiotic targets has widely varying effects on growth. Proc Natl Acad Sci U S A. 2011 Mar 8; 108(10):4176-81. PMID: 21368134.
      View in: PubMed
    59. Fong SS, Rearden P, Kanchagar C, Sassetti C, Trevejo J, Brereton RG. Automated peak detection and matching algorithm for gas chromatography-differential mobility spectrometry. Anal Chem. 2011 Mar 1; 83(5):1537-46. PMID: 21204557.
      View in: PubMed
    60. Sassetti CM, Rubin EJ. Relics of selection in the mycobacterial genome. Nat Genet. 2010 Jun; 42(6):476-8. PMID: 20502489.
      View in: PubMed
    61. Ioerger TR, Feng Y, Ganesula K, Chen X, Dobos KM, Fortune S, Jacobs WR, Mizrahi V, Parish T, Rubin E, Sassetti C, Sacchettini JC. Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories. J Bacteriol. 2010 Jul; 192(14):3645-53. PMID: 20472797.
      View in: PubMed
    62. Murry JP, Pandey AK, Sassetti CM, Rubin EJ. Phthiocerol dimycocerosate transport is required for resisting interferon-gamma-independent immunity. J Infect Dis. 2009 Sep 1; 200(5):774-82. PMID: 19622047.
      View in: PubMed
    63. Coulombe F, Divangahi M, Veyrier F, de Léséleuc L, Gleason JL, Yang Y, Kelliher MA, Pandey AK, Sassetti CM, Reed MB, Behr MA. Increased NOD2-mediated recognition of N-glycolyl muramyl dipeptide. J Exp Med. 2009 Aug 3; 206(8):1709-16. PMID: 19581406.
      View in: PubMed
    64. Pandey AK, Yang Y, Jiang Z, Fortune SM, Coulombe F, Behr MA, Fitzgerald KA, Sassetti CM, Kelliher MA. NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to Mycobacterium tuberculosis. PLoS Pathog. 2009 Jul; 5(7):e1000500. PMID: 19578435.
      View in: PubMed
    65. Chang JC, Miner MD, Pandey AK, Gill WP, Harik NS, Sassetti CM, Sherman DR. igr Genes and Mycobacterium tuberculosis cholesterol metabolism. J Bacteriol. 2009 Aug; 191(16):5232-9. PMID: 19542286.
      View in: PubMed
    66. Miner MD, Chang JC, Pandey AK, Sassetti CM, Sherman DR. Role of cholesterol in Mycobacterium tuberculosis infection. Indian J Exp Biol. 2009 Jun; 47(6):407-11. PMID: 19634704.
      View in: PubMed
    67. Sassetti CM. Inducible expression systems for mycobacteria. Methods Mol Biol. 2009; 465:255-64. PMID: 20560072.
      View in: PubMed
    68. Pandey AK, Raman S, Proff R, Joshi S, Kang CM, Rubin EJ, Husson RN, Sassetti CM. Nitrile-inducible gene expression in mycobacteria. Tuberculosis (Edinb). 2009 Jan; 89(1):12-6. PMID: 18801704.
      View in: PubMed
    69. Pandey AK, Sassetti CM. Mycobacterial persistence requires the utilization of host cholesterol. Proc Natl Acad Sci U S A. 2008 Mar 18; 105(11):4376-80. PMID: 18334639.
      View in: PubMed
    70. Murry JP, Sassetti CM, Lane JM, Xie Z, Rubin EJ. Transposon site hybridization in Mycobacterium tuberculosis. Methods Mol Biol. 2008; 416:45-59. PMID: 18392960.
      View in: PubMed
    71. Yang Y, Yin C, Pandey A, Abbott D, Sassetti C, Kelliher MA. NOD2 pathway activation by MDP or Mycobacterium tuberculosis infection involves the stable polyubiquitination of Rip2. J Biol Chem. 2007 Dec 14; 282(50):36223-9. PMID: 17947236.
      View in: PubMed
    72. Sassetti CM, Rubin EJ. The open book of infectious diseases. Nat Med. 2007 Mar; 13(3):279-80. PMID: 17342136.
      View in: PubMed
    73. Joshi SM, Pandey AK, Capite N, Fortune SM, Rubin EJ, Sassetti CM. Characterization of mycobacterial virulence genes through genetic interaction mapping. Proc Natl Acad Sci U S A. 2006 Aug 1; 103(31):11760-5. PMID: 16868085.
      View in: PubMed
    74. Murry J, Sassetti CM, Moreira J, Lane J, Rubin EJ. A new site-specific integration system for mycobacteria. Tuberculosis (Edinb). 2005 Sep-Nov; 85(5-6):317-23. PMID: 16256438.
      View in: PubMed
    75. Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman DR, Bloom BR, Rubin EJ. Mutually dependent secretion of proteins required for mycobacterial virulence. Proc Natl Acad Sci U S A. 2005 Jul 26; 102(30):10676-81. PMID: 16030141.
      View in: PubMed
    76. Rengarajan J, Sassetti CM, Naroditskaya V, Sloutsky A, Bloom BR, Rubin EJ. The folate pathway is a target for resistance to the drug para-aminosalicylic acid (PAS) in mycobacteria. Mol Microbiol. 2004 Jul; 53(1):275-82. PMID: 15225321.
      View in: PubMed
    77. Sassetti CM, Rubin EJ. Genetic requirements for mycobacterial survival during infection. Proc Natl Acad Sci U S A. 2003 Oct 28; 100(22):12989-94. PMID: 14569030.
      View in: PubMed
    78. Fieger CB, Sassetti CM, Rosen SD. Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x. J Biol Chem. 2003 Jul 25; 278(30):27390-8. PMID: 12889478.
      View in: PubMed
    79. Sassetti CM, Boyd DH, Rubin EJ. Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol. 2003 Apr; 48(1):77-84. PMID: 12657046.
      View in: PubMed
    80. Sassetti C, Rubin EJ. Genomic analyses of microbial virulence. Curr Opin Microbiol. 2002 Feb; 5(1):27-32. PMID: 11834365.
      View in: PubMed
    81. Sassetti CM, Boyd DH, Rubin EJ. Comprehensive identification of conditionally essential genes in mycobacteria. Proc Natl Acad Sci U S A. 2001 Oct 23; 98(22):12712-7. PMID: 11606763.
      View in: PubMed
    82. Sassetti C, Van Zante A, Rosen SD. Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins. J Biol Chem. 2000 Mar 24; 275(12):9001-10. PMID: 10722749.
      View in: PubMed
    83. Papadopoulos EJ, Sassetti C, Saeki H, Yamada N, Kawamura T, Fitzhugh DJ, Saraf MA, Schall T, Blauvelt A, Rosen SD, Hwang ST. Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 1999 Aug; 29(8):2551-9. PMID: 10458770.
      View in: PubMed
    84. Sassetti C, Tangemann K, Singer MS, Kershaw DB, Rosen SD. Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34. J Exp Med. 1998 Jun 15; 187(12):1965-75. PMID: 9625756.
      View in: PubMed
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