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One or more keywords matched the following properties of Royer, William
Rotation Projects

Potential Rotation Projects

Project #1: Probing the structural basis for cooperativity in a dimeric hemoglobin: Scapharca dimeric hemoglobin is an elegantly simple model system for exploring the structural basis for intersubunit communication. Our analysis to date has established a new paradigm for cooperativity in which tightly bound water molecules are used as sensors for ligand state.

In this project, the student will first mutate the gene for the native hemoglobin at a residue that is suspected of playing a role in the intersubunit communication. The mutated protein will then be expressed in E. coli and purified and subjected to functional analysis of oxygen binding. Using conditions already established for the native protein, the mutant hemoglobin will then be crystallized and subjected to preliminary x-ray analysis.

This project will provide the student with an introduction to two of the most powerful techniques for investigating the structure and function of proteins: site-directed mutagenesis and x-ray crystallography. Additionally, valuable experience in protein purification will be obtained.

Project #2: Structural analysis of the polymerization of sickle-cell hemoglobin Sickle cell disease results from the pathological polymerization of deoxygenated hemoglobin S (ß6E->V) within erythrocytes. This polymerization depends upon a complicated interplay of multiple interactions between tetramers. Our high resolution crystal structure of this molecule has elucidated details of some of the important interactions. Several projects are available for rotation students. These include crystallization of sickle-cell hemoglobin in the presence of an inhibitor to identify its binding site, site-directed mutagenesis of hemoglobin S and crystallization of mutants that show altered polymerization characteristics.

One or more keywords matched the following items that are connected to Royer, William
Item TypeName
Academic Article Crystal structure of deoxy-human hemoglobin beta6 Glu --> Trp. Implications for the structure and formation of the sickle cell fiber.
Academic Article Mutational destabilization of the critical interface water cluster in Scapharca dimeric hemoglobin: structural basis for altered allosteric activity.
Academic Article The 2.7 A crystal structure of deoxygenated hemoglobin from the sea lamprey (Petromyzon marinus): structural basis for a lowered oxygen affinity and Bohr effect.
Academic Article Crystal structure of the hemoglobin dodecamer from Lumbricus erythrocruorin: allosteric core of giant annelid respiratory complexes.
Academic Article X-Ray crystal structure and molecular dynamics simulations of silver hake parvalbumin (Isoform B).
Academic Article Allosteric action in real time: time-resolved crystallographic studies of a cooperative dimeric hemoglobin.
Academic Article Competition between Ski and CREB-binding protein for binding to Smad proteins in transforming growth factor-beta signaling.
Academic Article Ligand migration and binding in the dimeric hemoglobin of Scapharca inaequivalvis.
Academic Article Time-resolved x-ray crystallography of heme proteins.
Academic Article Insights into interferon regulatory factor activation from the crystal structure of dimeric IRF5.
Academic Article Ligand-linked structural transitions in crystals of a cooperative dimeric hemoglobin.
Academic Article Catalytic implications from the Drosophila protein L-isoaspartyl methyltransferase structure and site-directed mutagenesis.
Academic Article The 2.0 A crystal structure of Scapharca tetrameric hemoglobin: cooperative dimers within an allosteric tetramer.
Academic Article Crystal structure of oxygenated Scapharca dimeric hemoglobin at 1.7-A resolution.
Academic Article Residue F4 plays a key role in modulating oxygen affinity and cooperativity in Scapharca dimeric hemoglobin.
Academic Article Low resolution crystal structure of Arenicola erythrocruorin: influence of coiled coils on the architecture of a megadalton respiratory protein.
Academic Article Drug resistance against HCV NS3/4A inhibitors is defined by the balance of substrate recognition versus inhibitor binding.
Academic Article Crystals of Lumbricus erythrocruorin.
Academic Article High-resolution crystallographic analysis of a co-operative dimeric hemoglobin.
Concept Crystallography, X-Ray
Academic Article Tertiary and quaternary allostery in tetrameric hemoglobin from Scapharca inaequivalvis.
Academic Article Crystal structures of human CtBP in complex with substrate MTOB reveal active site features useful for inhibitor design.
Academic Article Structure-guided design of a high affinity inhibitor to human CtBP.
Academic Article Crystal structure of APOBEC3A bound to single-stranded DNA reveals structural basis for cytidine deamination and specificity.
Academic Article Assembly of human C-terminal binding protein (CtBP) into tetramers.
Academic Article Optimizing the refinement of merohedrally twinned P61 HIV-1 protease-inhibitor cocrystal structures.
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  • Crystallography X Ray