Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

Descriptor ID	D036641
MeSH Number(s)	E01.370.350.515.458.500 E01.370.350.515.717.250 E05.595.458.500 E05.595.717.250
Concept/Terms	Microscopy, Fluorescence, Multiphoton Microscopy, Fluorescence, Multiphoton Multiphoton Fluorescence Microscopy Microscopy, Multiphoton Fluorescence Fluorescence Microscopy, Multiphoton Multiphoton Excitation Microscopy Excitation Microscopies, Multiphoton Excitation Microscopy, Multiphoton Microscopies, Multiphoton Excitation Microscopy, Multiphoton Excitation Multiphoton Excitation Microscopies

Below are MeSH descriptors whose meaning is more general than "Microscopy, Fluorescence, Multiphoton".

Below are MeSH descriptors whose meaning is related to "Microscopy, Fluorescence, Multiphoton".

Below are MeSH descriptors whose meaning is more specific than "Microscopy, Fluorescence, Multiphoton".