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Jaime Rivera Perez PhD

TitleAssociate Professor
InstitutionUniversity of Massachusetts Medical School
DepartmentPediatrics
AddressUniversity of Massachusetts Medical School
55 Lake Avenue North
Worcester MA 01655
Phone508-856-4189
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentPediatrics

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentCell Biology


    Collapse Biography 
    Collapse education and training
    Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, NL, MexicoBSCBiology
    University of Texas Health Sciences Center, Houston, Houston, TX, United StatesPHDBiomedical Sciences
    Case Western Reserve University, Cleveland, OH, United StatesPostDocMammalian Development
    University of North Carolina, Chapel Hill, NC, United StatesPostDocMammalian Development

    Collapse Overview 
    Collapse overview

    Academic Background

    Jaime Rivera received his B.Sc. in Biology from the Universidad Autónoma de Nuevo León, in Monterrey, México in 1990. He obtained his Ph.D. from the University of Texas at Houston in 1997 under the direction of Richard Behringer. He then pursued his post-doctoral training with Terry Magnuson first at Case Western Reserve University and later at the University of North Carolina at Chapel Hill. He joined the faculty in the Department of Cell Biology at the University of Massachusetts Medical School in August 2006.

    Morphogenetic and Molecular Analysis of Mammalian Development

    Our research is centered in understanding the morphogenetic processes and the molecules that guide the development of the mammalian embryo from pre-implantation stages to the initial stages of organogenesis using the mouse as a model system. Our rationale is that understanding the basic mechanisms that guide cells and tissues during early embryogenesis is relevant not only in understanding normal development or what goes wrong during abnormal development but also in the advancement of research fields that include cancer and stem cells.

    We employ a variety of embryological, cellular, molecular and genetic approaches. These include lineage analysis, chimeras and embryo culture as well as time-lapse imaging, tissue specific knockouts, wholemount in situ hybridization and immunofluorescence. We also utilize embryonic stem cells for gene targeting.

    We have previously shown that the mouse conceptus at early post-implantation stages is a dynamic and molecularly patterned structure. Our studies demonstrated that the extra-embryonic component of the developing conceptus, particularly the visceral endoderm, undergoes dramatic cell movements and is composed of morphological and molecularly distinct cell populations. These studies provide evidence that interactions between embryonic and extra-embryonic tissues play a crucial role in shaping the mouse embryo. We are defining the role that these embryonic/extra-embryonic interactions play in patterning the embryo.

    One focus of our laboratory is to understand how and when the primary body axes of the embryo -the anteroposterior, dorsoventral and mediolateral axes- are established during embryogenesis. Currently, we are determining the role of Wnt3 in the development of the primitive streak and establishment of the anteroposterior axis.

    A hallmark of our research is the use of clonal analysis in cell fate determination. Clonal analysis permits assessment of the position and nature of the descendants of a single labeled cell. These studies are fundamental for understanding how pluripotent cells choose a particular fate as the embryo progresses in development. We are using this technique to determine the spatial relationships between cells that signal the development of organs as diverse as the brain, heart, placenta and endodermal derivatives such as the liver and pancreas.

    The importance of the early stages of embryogenesis for proper embryo development is underscored by the fact that thirty percent of human pregnancies are lost at early post-implantation stages. We strongly advocate fundamental research in early embryogenesis and are confident that our basic understanding of how the embryo develops will translate to clinical applications.

    Figures

    Rivera Figure 1

    Figure 1. Expression of Wnt3 in embryos between 5.5 and 6.5 days of gestation. The expression of Wnt3 in the posterior visceral endoderm and subsequently posterior epiblast marks the location and polarity of the anteroposterior axis as early as 5.5 dpc.
     
    Rivera Figure 2
    Figure 2. Clonal analysis of the trophoblast component of the blastocyst.A polar trophectoderm cell from a blastocyst derived from the ROSA26-LacZ reporter line, was injected with an expression vector coding for Cre recombinase and Rhodamine dextran (B).The labeled embryo was transferred into a pseudopregnant female and dissected at 6.5 dpc.ß-galactosidase positive descendants (blue), indicating Cre/lox recombination, are visible in the ectoplancental cone region of the embryo (C).
     
    Rivera Figure 3

    Figure 3. Molecular differences in the visceral endoderm layer of the conceptus. Egg cylinder stage embryo dissected at 5.75 days post-coitum. The promoter ofHhex, a homeobox containing gene, drives expression of green fluorescent protein in the anterior visceral endoderm (AVE). Red fluorescence marks cell-cell boundaries revealed by an anti-E-Cadherin antibody. The nuclei shown in blue are stained with DAPI.

     



    Collapse Rotation Projects

    Rotations Available

    Rotations are available, if interest contact Dr. Rivera directly atjaime.rivera@umassmed.edu


    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Conine CC, Sun F, Song L, Rivera-Pérez JA, Rando OJ. Small RNAs Gained during Epididymal Transit of Sperm Are Essential for Embryonic Development in Mice. Dev Cell. 2018 08 20; 46(4):470-480.e3. PMID: 30057276.
      View in: PubMed
    2. Yoon Y, Wang D, Tai PWL, Riley J, Gao G, Rivera-Pérez JA. Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun. 2018 01 29; 9(1):412. PMID: 29379011.
      View in: PubMed
    3. Acharya D, Hainer SJ, Yoon Y, Wang F, Bach I, Rivera-Pérez JA, Fazzio TG. KAT-Independent Gene Regulation by Tip60 Promotes ESC Self-Renewal but Not Pluripotency. Cell Rep. 2017 Apr 25; 19(4):671-679. PMID: 28445719.
      View in: PubMed
    4. Yoon Y, Huang T, Tortelote GG, Wakamiya M, Hadjantonakis AK, Behringer RR, Rivera-Pérez JA. Extra-embryonic Wnt3 regulates the establishment of the primitive streak in mice. Dev Biol. 2015 Jul 01; 403(1):80-8. PMID: 25907228.
      View in: PubMed
    5. Rivera-Pérez JA, Hadjantonakis AK. The Dynamics of Morphogenesis in the Early Mouse Embryo. Cold Spring Harb Perspect Biol. 2014 Jun 26; 7(11). PMID: 24968703.
      View in: PubMed
    6. Huang T, Rivera-Pérez JA. Senescence-associated ß-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence. Genesis. 2014 Apr; 52(4):300-8. PMID: 24616249.
      View in: PubMed
    7. Sundararajan S, Wakamiya M, Behringer RR, Rivera-Pérez JA. A fast and sensitive alternative for ß-galactosidase detection in mouse embryos. Development. 2012 Dec 01; 139(23):4484-90. PMID: 23132248.
      View in: PubMed
    8. Tortelote GG, Hernández-Hernández JM, Quaresma AJ, Nickerson JA, Imbalzano AN, Rivera-Pérez JA. Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice. Dev Biol. 2013 Feb 1; 374(1):164-73. PMID: 23085236.
      View in: PubMed
    9. Yoon Y, Cowley DO, Gallant J, Jones SN, Van Dyke T, Rivera-Pérez JA. Conditional Aurora A deficiency differentially affects early mouse embryo patterning. Dev Biol. 2012 Nov 1; 371(1):77-85. PMID: 22939930.
      View in: PubMed
    10. Rivera-Pérez JA, Jones V, Tam PP. Culture of whole mouse embryos at early postimplantation to organogenesis stages: developmental staging and methods. Methods Enzymol. 2010; 476:185-203. PMID: 20691867.
      View in: PubMed
    11. Rivera-Perez JA. Axial specification in mice: ten years of advances and controversies. J Cell Physiol. 2007 Dec; 213(3):654-60. PMID: 17893928.
      View in: PubMed
    12. Rivera-Pérez JA, Magnuson T. Primitive streak formation in mice is preceded by localized activation of Brachyury and Wnt3. Dev Biol. 2005 Dec 15; 288(2):363-71. PMID: 16289026.
      View in: PubMed
    13. Rivera-Pérez JA, Mager J, Magnuson T. Dynamic morphogenetic events characterize the mouse visceral endoderm. Dev Biol. 2003 Sep 15; 261(2):470-87. PMID: 14499654.
      View in: PubMed
    14. Rivera-Pérez JA, Wakamiya M, Behringer RR. Goosecoid acts cell autonomously in mesenchyme-derived tissues during craniofacial development. Development. 1999 Sep; 126(17):3811-21. PMID: 10433910.
      View in: PubMed
    15. Rivera-Pérez JA, Mallo M, Gendron-Maguire M, Gridley T, Behringer RR. Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development. Development. 1995 Sep; 121(9):3005-12. PMID: 7555726.
      View in: PubMed
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