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Martin G Marinus PHD

TitleProfessor Emeritus
InstitutionUMass Chan Medical School
DepartmentBiochemistry and Molecular Biotechnology
AddressUMass Chan Medical School
364 Plantation Street LRB
Worcester MA 01605
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionUMass Chan Programs, Centers and Institutes
    DepartmentBacterial Genetics and Pathogenesis

    Collapse Biography 
    Collapse education and training
    University of Otago, Dunedin, , New ZealandBSCMicrobiology
    University of Otago, Dunedin, , New ZealandPHDMicrobiology
    Collapse awards and honors
    1975 - 1980Faculty Research Award, American Cancer Society
    2005Elected to Fellowship, American Academy of Microbiology

    Collapse Overview 
    Collapse overview

    Academic Background

    B.Sc.(Hons.), 1965, PhD, Otago, New Zealand, 1968
    American Cancer Society Faculty Research Award, 1976-1981
    Visiting Professor, University of Sussex, UK 1980-1981
    Erskine Fellow, University of Canterbury, NZ 1991
    Visiting Professor, University of Goettingen, Germany 1996-1997
    Visiting Professor, Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette 2006


    Biology of Enterohemorrhagic Escherichia coli O157:H7 and Enterovirus 933W.
      Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is among the leading causes of food- and water-borne illnesses affecting humans in the U.S., Europe, and Japan. Although most EHEC infections resolve spontaneously after 5-10 days of abdominal cramping and bloody diarrhea, approximately 2-7% of cases progress to the potentially fatal hemolytic uremic syndrome due, in part, to the production of cytotoxic Shiga toxins which are capable of promoting kidney failure.
     We found that resistance to certain antibiotics in E. coli O157:H7 occurs at a higher frequency than in E. coli K-12. The mutation responsible is in the mar (multiple antibiotic resistance) genes. The molecular basis for the higher mutation rate in these genes is still unknown.
     In EHEC strain EDL933, the genes (stx2AB) encoding Shiga toxin are located on prophage 933W in the late region between genes Q and S and are transcribed with the late region genes from promoter PR’. Previous work showed that the ends of the genome in a free phage population could not be sequenced suggesting that they are terminally redundant. If so, then 933W should be able to carry out transduction of host genes from one bacterium to another. We showed that, indeed, this is the case thereby providing another way to transfer genetic material among EHEC strains.Current work includes using 933W as an indicator of SOS induction.

    Formation of F-actin in HeLa cells by Escherichia coli 0157:H7. A HeLa cell infected with dam mutant bacteria of E. coli 0157:H7 is shown stained with DAPI (bacteria), filamentous actin stain (pedestals) and a merged image.

    Owing to circumstances beyond my control, bacterial strains and plasmids are no longer available. Please contact the E. coli Genetic Stock Center which may be able to help you with bacterial strains. Addgene may be able to provide plasmids.

    Microarray AnalysisMicroarray results show that global gene expression in a seqA mutant strain (left panel) is the same as that in a wildtype strain overproducing the Dam methyltransferase (center panel) but that neither is similar to that for the dam mutant (right panel).

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Carone BR, Xu T, Murphy KC, Marinus MG. High incidence of multiple antibiotic resistant cells in cultures of in enterohemorrhagic Escherichia coli O157:H7. Mutat Res. 2014 Jan; 759:1-8. PMID: 24361397.
      Citations: 8     Fields:    Translation:Cells
    2. Groothuizen FS, Fish A, Petoukhov MV, Reumer A, Manelyte L, Winterwerp HH, Marinus MG, Lebbink JH, Svergun DI, Friedhoff P, Sixma TK. Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res. 2013 Sep; 41(17):8166-81. PMID: 23821665.
      Citations: 16     Fields:    Translation:Cells
    3. Jakobsen H, Bojer MS, Marinus MG, Xu T, Struve C, Krogfelt KA, L?bner-Olesen A. The alkaloid compound harmane increases the lifespan of Caenorhabditis elegans during bacterial infection, by modulating the nematode's innate immune response. PLoS One. 2013; 8(3):e60519. PMID: 23544153.
      Citations: 13     Fields:    Translation:HumansAnimalsCells
    4. Marinus MG, Poteete AR. High efficiency generalized transduction in Escherichia coli O157:H7. F1000Res. 2013; 2. PMID: 24327879.
    5. Marinus, M.G. DNA Mismatch Repair. EcoSal-Escherichia coli and Salmonella: cellular and molecular biology. A. Böck, R. Curtiss III, J. B. Kaper, P. D. Karp, F. C. Neidhardt, T. Nyström, J. M. Slauch, C. L. Squires, and D. Ussery (ed.). 2012. View Publication.
    6. Murphy KC, Marinus MG. RecA-independent single-stranded DNA oligonucleotide-mediated mutagenesis. F1000 Biol Rep. 2010 Jul 22; 2:56. PMID: 20711416.
    7. Marinus MG. DNA methylation and mutator genes in Escherichia coli K-12. Mutat Res. 2010 Oct; 705(2):71-76. PMID: 20471491.
      Citations: 22     Fields:    Translation:Cells
    8. Marinus MG, Casadesus J. Roles of DNA adenine methylation in host-pathogen interactions: mismatch repair, transcriptional regulation, and more. FEMS Microbiol Rev. 2009 May; 33(3):488-503. PMID: 19175412.
      Citations: 131     Fields:    Translation:Cells
    9. L?bner-Olesen A, Slominska-Wojewodzka M, Hansen FG, Marinus MG. DnaC inactivation in Escherichia coli K-12 induces the SOS response and expression of nucleotide biosynthesis genes. PLoS One. 2008 Aug 20; 3(8):e2984. PMID: 18714349.
      Citations: 4     Fields:    Translation:Cells
    10. Broadbent SE, Balbontin R, Casadesus J, Marinus MG, van der Woude M. YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica. J Bacteriol. 2007 Jun; 189(11):4325-7. PMID: 17400740.
      Citations: 17     Fields:    Translation:Cells
    11. Riber L, Olsson JA, Jensen RB, Skovgaard O, Dasgupta S, Marinus MG, L?bner-Olesen A. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes Dev. 2006 Aug 01; 20(15):2121-34. PMID: 16882985.
      Citations: 50     Fields:    Translation:Cells
    12. L?pez de Saro FJ, Marinus MG, Modrich P, O'Donnell M. The beta sliding clamp binds to multiple sites within MutL and MutS. J Biol Chem. 2006 May 19; 281(20):14340-9. PMID: 16546997.
      Citations: 44     Fields:    Translation:HumansCells
    13. Robbins-Manke JL, Zdraveski ZZ, Marinus M, Essigmann JM. Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli. J Bacteriol. 2005 Oct; 187(20):7027-37. PMID: 16199573.
      Citations: 45     Fields:    Translation:Cells
    14. L?bner-Olesen A, Skovgaard O, Marinus MG. Dam methylation: coordinating cellular processes. Curr Opin Microbiol. 2005 Apr; 8(2):154-60. PMID: 15802246.
      Citations: 115     Fields:    Translation:Cells
    15. L?bner-Olesen A, Marinus MG, Hansen FG. Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis. Proc Natl Acad Sci U S A. 2003 Apr 15; 100(8):4672-7. PMID: 12682301.
      Citations: 65     Fields:    Translation:Cells
    16. Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Kr?ger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 01; 31(7):1805-12. PMID: 12654995.
      Citations: 349     Fields:    Translation:Cells
    17. Zdraveski ZZ, Mello JA, Marinus MG, Essigmann JM. Multiple pathways of recombination define cellular responses to cisplatin. Chem Biol. 2000 Jan; 7(1):39-50. PMID: 10662689.
      Citations: 29     Fields:    Translation:Cells
    18. Marinus MG. Recombination is essential for viability of an Escherichia coli dam (DNA adenine methyltransferase) mutant. J Bacteriol. 2000 Jan; 182(2):463-8. PMID: 10629194.
      Citations: 31     Fields:    Translation:Cells
    19. Rasmussen LJ, L?bner-Olesen A, Marinus MG. Growth-rate-dependent transcription initiation from the dam P2 promoter. Gene. 1995 May 19; 157(1-2):213-5. PMID: 7607492.
      Citations: 8     Fields:    Translation:HumansCells
    20. Carraway M, Marinus MG. Repair of heteroduplex DNA molecules with multibase loops in Escherichia coli. J Bacteriol. 1993 Jul; 175(13):3972-80. PMID: 8320213.
      Citations: 16     Fields:    Translation:Cells
    21. Foster PL, Marinus MG. Levels of epsilon, an essential replication subunit of Escherichia coli DNA polymerase III, are controlled by heat shock proteins. J Bacteriol. 1992 Dec; 174(23):7509-16. PMID: 1332935.
      Citations: 11     Fields:    Translation:Cells
    22. Parker BO, Marinus MG. Repair of DNA heteroduplexes containing small heterologous sequences in Escherichia coli. Proc Natl Acad Sci U S A. 1992 Mar 01; 89(5):1730-4. PMID: 1542666.
      Citations: 82     Fields:    Translation:Cells
    23. Boye E, Marinus MG, L?bner-Olesen A. Quantitation of Dam methyltransferase in Escherichia coli. J Bacteriol. 1992 Mar; 174(5):1682-5. PMID: 1537808.
      Citations: 17     Fields:    Translation:Cells
    24. L?bner-Olesen A, Marinus MG. Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. J Bacteriol. 1992 Jan; 174(2):525-9. PMID: 1309529.
      Citations: 22     Fields:    Translation:Cells
    25. Boe L, Marinus MG. Role of plasmid multimers in mutation to tetracycline resistance. Mol Microbiol. 1991 Oct; 5(10):2541-5. PMID: 1791764.
      Citations: 6     Fields:    Translation:Cells
    26. Barras F, Marinus MG. Arrangement of Dam methylation sites (GATC) in the Escherichia coli chromosome. Nucleic Acids Res. 1988 Oct 25; 16(20):9821-38. PMID: 3054812.
      Citations: 14     Fields:    Translation:Cells
    27. Fram RJ, Cusick PS, Wilson JM, Marinus MG. Mismatch repair of cis-diamminedichloroplatinum(II)-induced DNA damage. Mol Pharmacol. 1985 Jul; 28(1):51-5. PMID: 3894930.
      Citations: 26     Fields:    Translation:Cells
    28. Peterson KR, Wertman KF, Mount DW, Marinus MG. Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon. Mol Gen Genet. 1985; 201(1):14-9. PMID: 3932821.
      Citations: 43     Fields:    Translation:Cells
    29. Marinus MG, Poteete A, Arraj JA. Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12. Gene. 1984 Apr; 28(1):123-5. PMID: 6376282.
      Citations: 59     Fields:    Translation:Cells
    30. Arraj JA, Marinus MG. Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene. J Bacteriol. 1983 Jan; 153(1):562-5. PMID: 6336742.
      Citations: 36     Fields:    Translation:Cells
    31. Karran P, Marinus MG. Mismatch correction at O6-methylguanine residues in E. coli DNA. Nature. 1982 Apr 29; 296(5860):868-9. PMID: 7040986.
      Citations: 76     Fields:    Translation:Cells
    32. McGraw BR, Marinus MG. Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12. Mol Gen Genet. 1980; 178(2):309-15. PMID: 6993844.
      Citations: 48     Fields:    Translation:Cells
    33. Bale A, d'Alarcao M, Marinus MG. Characterization of DNA adenine methylation mutants of Escherichia coli K12. Mutat Res. 1979 Feb; 59(2):157-65. PMID: 375073.
      Citations: 51     Fields:    Translation:Cells
    34. Marinus MG, Konrad EB. Hyper-recombination in dam mutants of Escherichia coli K-12. Mol Gen Genet. 1976 Dec 22; 149(3):273-7. PMID: 799245.
      Citations: 37     Fields:    Translation:Cells
    35. Marinus MG, Morris NR. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res. 1975 Apr; 28(1):15-26. PMID: 167279.
      Citations: 85     Fields:    Translation:Cells
    36. Marinus MG, Morris NR. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12. J Mol Biol. 1974 May 15; 85(2):309-22. PMID: 4600143.
      Citations: 107     Fields:    Translation:Cells
    37. Marinus MG, Adelberg EA. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. J Bacteriol. 1970 Dec; 104(3):1266-72. PMID: 16559102.
      Citations: 19     Fields:    
    38. Marinus MG, Loutit JS. Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. I. Characterisation and mapping of mutants. Genetics. 1969 Nov; 63(3):547-56. PMID: 4992231.
      Citations: 10     Fields:    Translation:Cells
    39. Loutit JS, Marinus MG. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet Res. 1968 Aug; 12(1):37-44. PMID: 4976997.
      Citations: 13     Fields:    Translation:Cells
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