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Yang Xiang PhD

TitleAssociate Professor
InstitutionUMass Chan Medical School
DepartmentNeurobiology
AddressUMass Chan Medical School
364 Plantation Street LRB 725
Worcester MA 01605
Phone508-856-6148
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentNeurobiology

    InstitutionT.H. Chan School of Medicine
    DepartmentNeuroNexus Institute

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentNeuroscience

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentTranslational Science


    Collapse Biography 
    Collapse education and training
    Nanjing University, Nanjing, , ChinaBSBiochemistry
    Chinese Academy of Sciences, Beijing, , ChinaPHDNeurobiology

    Collapse Overview 
    Collapse overview


    Biography



    Yang Xiang received his B.S. in Biochemistry from Nanjing University in 1999. He carried out his doctoral training in the laboratory of Dr. Mu-ming Poo at the institute of Neuroscience, Chinese Academy of Sciences, where he studied axonal guidance, obtained his Ph.D in Neurobiology in 2003. Yang Xiang then received the Human Frontiers Science Program fellowship to work as a postdoctoral associate with Dr. Yuh-Nung Jan at HHMI, University of California San Francisco from 2004-2012, studying sensory neuron function in Drosophila. He started his laboratory in the Department of Neurobiology at The University of Massachusetts Medical School in 2012.



    Molecular and neural circuit mechanisms underlying behavior



    Our lab is interested in understanding how the nervous system detects and processes sensory stimuli to generate appropriate behavior, a central question in neurobiology. We work on the genetically tractable organism Drosophila. Specifically, our research focuses on the innate escape behavior triggered by harmful light in Drosophila larvae. We found that a specific class of sensory neurons (namely, class IV dendritic arborization (da) neurons) are novel photoreceptors activated by intense harmful short wavelength light, with spectral sensitivity UV>violet>blue>>green~red, and mediate larval escape behavior when illuminated by UV, violet and blue light. Classical phototransduction molecules are not required in class IV da neurons. Instead, gustatory receptor 28b (Gr28b), G protein signaling and dTRPA1 are critical. Beyond harmful light, class IV da neurons also sense noxious heat (>40oC) and the irritant chemical AITC, a major component of wasabi.



    Applying molecular genetics, GCaMP-based functional imaging, electrophysiology, and behavioral assay, our goal is to dissect the molecular, cellular and neural circuit mechanisms underlying sensory stimuli-induced behavior in Drosophila larvae. Specifically, we aim to (1) identify the phototransduction mechanism in class IV da neurons; (2) define and characterize the neural circuits underlying light-induced escape behavior. 



    People in the lab







































































    Postdoctoral Fellows


     




    Pengyu Gu, Ph.D.(Nanjing University)

     

     


    Student


    Tackle

    Kendra Takle, B.S.(University of Wisconsin, Madison)

     





    Yang Xiang photo 1


     



    Figure 1 Dorsal view of a 3rd instar Drosophila larva (5 mm in length) with class IV da neurons marked by ppk-CD4TdGFP. en-GAL4 in red marks the posterior epidermal cells of each segment. Each larva has 11 segments and there are 3 class IV da neurons in each hemi-segment. Dendrites of class IV da neurons cover the entire larval body wall in a complete but non-overlapping fashion, a phenomenon called dendritic tiling. This organization will ensure that larvae can detect strong harmful light exposure over their entire bodies. (Image Credit: Dr. Chun Han, UCSF)

     

     


    Yang Xiang photo 2



     



    Figure 2 class IV da neurons detect harmful UV to initiate the escape behavior. Our working model is that Gr28b absorbs photon, and leads to dTRPA1 channels opening and action potential firing in class IV da neurons. Electrical signals encoded by class IV da neurons will be decoded by neural circuits in the CNS into escape behavior.

     

     



    Get Flash player.




     





    Get Flash player.



    Movie 1 Upon 5 s light stimulation of the anterior region (0.57 mW/mm2 white light, comparable to sunlight intensity), a 3rd instar larva of wt (left) exhibits avoidance behavior, while a larva with class IV da neurons genetically ablated (right) doesn’t avoid light.

     


    Get Flash player.



    Movie 2 Class IV da neurons are specifically activated by UV (365 nm) and blue (470 nm), but not green (546 nm) light. Shown here are larval peripheral sensory neurons in a dorsal cluster expressing GCaMP3, with the arrow pointing to the class IV da neuron. 5 s light stimulation is indicated in the upper-right corner. GCaMP3 fluorescence is marked by pseudo-color, with red indicating high and blue indicating low intensity. Similar light activation of class IV da neurons is observed in lateral and ventral clusters across the entire larval body wall.

     

     


     



     


     



     


     

    Collapse Rotation Projects

    One rotation student is expected in the lab to characterize the expression and function of Drosophila gustatory receptor 28b (Gr28b) in phototransduction and taste sensation. Please contact Dr. Yang Xiang for details.

     

    Yang Xiang, Ph.D.
    University of Massachusetts Medical School
    Department of Neurobiology, LRB 725
    364 Plantation Street
    Worcester, MA 01605 USA
    phone: 508-856-6816 (office)
    e-mail:
    yang.xiang@umassmed.edu



    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Gong J, Nirala NK, Chen J, Wang F, Gu P, Wen Q, Ip YT, Xiang Y. TrpA1 is a shear stress mechanosensing channel regulating intestinal stem cell proliferation in Drosophila. Sci Adv. 2023 05 24; 9(21):eadc9660. PMID: 37224252.
      Citations: 2     Fields:    Translation:AnimalsCells
    2. Wang F, Ruppell KT, Zhou S, Qu Y, Gong J, Shang Y, Wu J, Liu X, Diao W, Li Y, Xiang Y. Gliotransmission and adenosine signaling promote axon regeneration. Dev Cell. 2023 04 24; 58(8):660-676.e7. PMID: 37028426.
      Citations: 1     Fields:    Translation:AnimalsCells
    3. Gong J, Chen J, Gu P, Shang Y, Ruppell KT, Yang Y, Wang F, Wen Q, Xiang Y. Shear stress activates nociceptors to drive Drosophila mechanical nociception. Neuron. 2022 Nov 16; 110(22):3727-3742.e8. PMID: 36087585.
      Citations: 4     Fields:    Translation:AnimalsCells
    4. Gu P, Wang F, Shang Y, Liu J, Gong J, Xie W, Han J, Xiang Y. Nociception and hypersensitivity involve distinct neurons and molecular transducers in Drosophila. Proc Natl Acad Sci U S A. 2022 03 22; 119(12):e2113645119. PMID: 35294287.
      Citations: 6     Fields:    Translation:AnimalsCells
    5. Gu P, Gong J, Shang Y, Wang F, Ruppell KT, Ma Z, Sheehan AE, Freeman MR, Xiang Y. Polymodal Nociception in Drosophila Requires Alternative Splicing of TrpA1. Curr Biol. 2019 12 02; 29(23):3961-3973.e6. PMID: 31735672.
      Citations: 18     Fields:    Translation:AnimalsCells
    6. Yang L, Li R, Kaneko T, Takle K, Morikawa RK, Essex L, Wang X, Zhou J, Emoto K, Xiang Y, Ye B. Trim9 regulates activity-dependent fine-scale topography in Drosophila. Curr Biol. 2014 May 05; 24(9):1024-30. PMID: 24746793.
      Citations: 17     Fields:    Translation:AnimalsCells
    7. Yan Z, Zhang W, He Y, Gorczyca D, Xiang Y, Cheng LE, Meltzer S, Jan LY, Jan YN. Drosophila NOMPC is a mechanotransduction channel subunit for gentle-touch sensation. Nature. 2013 Jan 10; 493(7431):221-5. PMID: 23222543.
      Citations: 153     Fields:    Translation:AnimalsCells
    8. Yan, Z. *, Zhang, W. *, He, Y., Gorczyca, D., Xiang, Y., Cheng, L., Meltzer, S., Jan, L.Y., & Jan, Y.N. NompC is a pore-forming subunit of a mechanotransduction channel for sensing gentle touch in Drosophila. Nature. 2012; (In press).
    9. Yuan, Q., Xiang, Y., Yan, Z.Q., Han, C., Jan, L.Y., & Jan, Y.N. Light induced structural and functional plasticity in Drosophila larval visual System. Science. 2011; 333:1458-1462.
    10. Xiang, Y., Yuan, Q., Vogt, N., Looger, L. L., Jan, L. Y. and Jan, Y. N. Light-avoidance-mediating photoreceptors tile the Drosophila larval body wall. Nature. 2010; 468:921-926. (research article).
    11. Yang, C., Rumpf, S., Xiang, Y., Gordon, M. D., Song, W., Scott, K., Jan, L. Y. and Jan, Y. N. . Control of post-mating behavior switch in Drosophila females by internal sensory neurons. Neuron. 2009; 61:519-526.
    12. Xiang, Y., Li, Y., Zhang, Z., Cui, K., Wang, S., Yuan, X., Wu, C., Poo, M. and Duan, S. Nerve growth cone guidance mediated by G protein-coupled receptors. Nature Neuroscience. 2002; 5:843-848.
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