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Sean P Ryder PhD

InstitutionUMass Chan Medical School
DepartmentBiochemistry and Molecular Biotechnology
AddressUMass Chan Medical School
364 Plantation Street LRB
Worcester MA 01605
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionT.H. Chan School of Medicine
    DepartmentNeuroNexus Institute

    InstitutionT.H. Chan School of Medicine
    DepartmentRNA Therapeutics Institute

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBiophysical Chemical and Computational Biology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionMorningside Graduate School of Biomedical Sciences

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentPostbaccalaureate Research Education Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentTranslational Science

    InstitutionUMass Chan Programs, Centers and Institutes
    DepartmentBioinformatics and Integrative Biology

    Collapse Biography 
    Collapse education and training
    University of New Hampshire at Manchester, Manchester, NH, United StatesB SBiochemistry
    Yale University, New Haven, CT, United StatesPH DMolecular Biophysics & Biochem

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    About the Ryder Lab:

    We are interested in understanding the mechanisms that govern post-transcriptional regulation of gene expression during development and complex disease. Our research is focused primarily in three areas:

    Ryder Lab Group Photo

    1. Post-transcriptional regulatory mechanisms involved in axis polarization and cell fate specification during Caenorhabditis elegans embryogenesis.

    2. Characterization of post-transcriptional regulatory mechanisms that guide oligodendrocyte differentiation and myelin formation in the vertebrate central nervous system.

    3. Identification of small molecule inhibitors of RNA-binding protein function using high throughput screening approaches.

    We employ a combination of biochemical and molecular genetic methods, bioinformatics, chemical biology, biophysics, and structural analyses in our studies, with a focus on quantitative measurements. Our laboratory is located on the 9th floor of the Lazare Research Building in the Department of Biochemistry and Molecular Pharmacology at the University of Massachusetts Medical School. We enjoy a collaborative research environment that includes colleagues with related interests in worm biology, RNA biochemistry, developmental biology, or biophysics. Read more about our research here.


    Collapse Rotation Projects

    Potential Rotation Projects

    A variety of rotation projects are available to study RNP assembly and post-transcriptional regulation during development using quantitative biochemical and modern molecular methods.

    Project 1: Characterization of the RNA-protein complexes that guide C. elegans early development. Several projects ranging in scope from biochemical characterization of RNA-binding protein complexes to genetic dissection of RNA binding protein function in development are currently available. Contact Sean for more details.

    Project 2: Post-transcriptional regulation of oligodendrocyte differentiation. Projects are available to monitor changes in gene expression as a function of differentiation with a focus on changes in alternative splicing and mRNA stability. Contact Sean for more details.

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    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Brown HE, Varderesian HV, Keane SA, Ryder SP. The mex-3 3' untranslated region is essential for reproduction during temperature stress. bioRxiv. 2024 Apr 01. PMID: 38798418.
    2. Antkowiak KR, Coskun P, Noronha ST, Tavella D, Massi F, Ryder SP. A nematode model to evaluate microdeletion phenotype expression. G3 (Bethesda). 2024 02 07; 14(2). PMID: 37956108.
      Citations:    Fields:    Translation:Animals
    3. Albarqi MMY, Ryder SP. The role of RNA-binding proteins in orchestrating germline development in Caenorhabditis elegans. Front Cell Dev Biol. 2022; 10:1094295. PMID: 36684428.
    4. Albarqi MMY, Ryder SP. The endogenous mex-3 3?UTR is required for germline repression and contributes to optimal fecundity in C. elegans. PLoS Genet. 2021 08; 17(8):e1009775. PMID: 34424904.
      Citations: 4     Fields:    Translation:AnimalsCells
    5. Ryder SP, Morgan BR, Coskun P, Antkowiak K, Massi F. Analysis of Emerging Variants in Structured Regions of the SARS-CoV-2 Genome. Evol Bioinform Online. 2021; 17:11769343211014167. PMID: 34017166.
    6. Ryder SP, Morgan BR, Massi F. Analysis of Rapidly Emerging Variants in Structured Regions of the SARS-CoV-2 Genome. bioRxiv. 2020 Jun 30. PMID: 32577650.
    7. Tavella D, Ertekin A, Schaal H, Ryder SP, Massi F. A Disorder-to-Order Transition Mediates RNA Binding of the Caenorhabditis elegans Protein MEX-5. Biophys J. 2020 04 21; 118(8):2001-2014. PMID: 32294479.
      Citations: 3     Fields:    Translation:AnimalsCells
    8. Hu S, Skelly LE, Kaymak E, Freeberg L, Lo TW, Kuersten S, Ryder SP, Haag ES. Multi-modal regulation of C. elegans hermaphrodite spermatogenesis by the GLD-1-FOG-2 complex. Dev Biol. 2019 02 15; 446(2):193-205. PMID: 30599151.
      Citations: 8     Fields:    Translation:Animals
    9. Ryder SP. #CRISPRbabies: Notes on a Scandal. CRISPR J. 2018 Dec 01; 1(6):355-357. PMID: 30687814.
      Citations: 5     Fields:    
    10. Han B, Antkowiak KR, Fan X, Rutigliano M, Ryder SP, Griffin EE. Polo-like Kinase Couples Cytoplasmic Protein Gradients in the C.?elegans Zygote. Curr Biol. 2018 01 08; 28(1):60-69.e8. PMID: 29276126.
      Citations: 23     Fields:    Translation:AnimalsCells
    11. Dowdle ME, Imboden SB, Park S, Ryder SP, Sheets MD. Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes. J Vis Exp. 2017 07 28; (125). PMID: 28784977.
      Citations: 5     Fields:    Translation:AnimalsCells
    12. Tamburino AM, Kaymak E, Shrestha S, Holdorf AD, Ryder SP, Walhout AJM. PRIMA: a gene-centered, RNA-to-protein method for mapping RNA-protein interactions. Translation (Austin). 2017; 5(1):e1295130. PMID: 28702278.
    13. Ryder SP. Protein-mRNA interactome capture: cartography of the mRNP landscape. F1000Res. 2016; 5:2627. PMID: 29098073.
      Citations: 6     Fields:    
    14. Thokala P, Simpson EL, Tappenden P, Stevens JW, Dickinson K, Ryder S, Harrison P. Ledipasvir-Sofosbuvir for Treating Chronic Hepatitis C: A NICE Single Technology Appraisal-An Evidence Review Group Perspective. Pharmacoeconomics. 2016 08; 34(8):741-50. PMID: 26892974.
      Citations: 3     Fields:    Translation:HumansCells
    15. Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP. Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI. Dev Dyn. 2016 09; 245(9):925-36. PMID: 27294288.
      Citations: 9     Fields:    Translation:AnimalsCells
    16. Elewa A, Shirayama M, Kaymak E, Harrison PF, Powell DR, Du Z, Chute CD, Woolf H, Yi D, Ishidate T, Srinivasan J, Bao Z, Beilharz TH, Ryder SP, Mello CC. POS-1 Promotes Endo-mesoderm Development by Inhibiting the Cytoplasmic Polyadenylation of neg-1 mRNA. Dev Cell. 2015 Jul 06; 34(1):108-18. PMID: 26096734.
      Citations: 11     Fields:    Translation:AnimalsCells
    17. Bohn MF, Shandilya SMD, Silvas TV, Nalivaika EA, Kouno T, Kelch BA, Ryder SP, Kurt-Yilmaz N, Somasundaran M, Schiffer CA. The ssDNA Mutator APOBEC3A Is Regulated by Cooperative Dimerization. Structure. 2015 May 05; 23(5):903-911. PMID: 25914058.
      Citations: 59     Fields:    Translation:HumansCells
    18. Ryder SP. Personal reflection (RNA journal 20th anniversary). RNA. 2015 Apr; 21(4):725-6. PMID: 25780210.
      Citations:    Fields:    
    19. Zearfoss NR, Deveau LM, Clingman CC, Schmidt E, Johnson ES, Massi F, Ryder SP. A conserved three-nucleotide core motif defines Musashi RNA binding specificity. J Biol Chem. 2014 Dec 19; 289(51):35530-41. PMID: 25368328.
      Citations: 47     Fields:    Translation:HumansAnimalsCells
    20. Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, Ryder SP. Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite. Elife. 2014 Jun 16; 3. PMID: 24935936.
      Citations: 37     Fields:    Translation:AnimalsCells
    21. Kaymak E, Ryder SP. RNA recognition by the Caenorhabditis elegans oocyte maturation determinant OMA-1. J Biol Chem. 2013 Oct 18; 288(42):30463-30472. PMID: 24014033.
      Citations: 19     Fields:    Translation:AnimalsCells
    22. Zearfoss NR, Johnson ES, Ryder SP. hnRNP A1 and secondary structure coordinate alternative splicing of Mag. RNA. 2013 Jul; 19(7):948-57. PMID: 23704325.
      Citations: 8     Fields:    Translation:AnimalsCells
    23. Clingman CC, Ryder SP. Metabolite sensing in eukaryotic mRNA biology. Wiley Interdiscip Rev RNA. 2013 Jul-Aug; 4(4):387-96. PMID: 23653333.
      Citations: 6     Fields:    Translation:AnimalsCells
    24. Tamburino AM, Ryder SP, Walhout AJ. A compendium of Caenorhabditis elegans RNA binding proteins predicts extensive regulation at multiple levels. G3 (Bethesda). 2013 Feb; 3(2):297-304. PMID: 23390605.
      Citations: 23     Fields:    Translation:AnimalsCells
    25. Farley BM, Ryder SP. POS-1 and GLD-1 repress glp-1 translation through a conserved binding-site cluster. Mol Biol Cell. 2012 Dec; 23(23):4473-83. PMID: 23034181.
      Citations: 13     Fields:    Translation:AnimalsCells
    26. Zearfoss NR, Ryder SP. End-labeling oligonucleotides with chemical tags after synthesis. Methods Mol Biol. 2012; 941:181-93. PMID: 23065562.
      Citations: 19     Fields:    
    27. Broderick JA, Salomon WE, Ryder SP, Aronin N, Zamore PD. Argonaute protein identity and pairing geometry determine cooperativity in mammalian RNA silencing. RNA. 2011 Oct; 17(10):1858-69. PMID: 21878547.
      Citations: 74     Fields:    Translation:HumansAnimalsCells
    28. Kalchhauser I, Farley BM, Pauli S, Ryder SP, Ciosk R. FBF represses the Cip/Kip cell-cycle inhibitor CKI-2 to promote self-renewal of germline stem cells in C. elegans. EMBO J. 2011 Aug 05; 30(18):3823-9. PMID: 21822213.
      Citations: 20     Fields:    Translation:AnimalsCells
    29. Ryder SP. Pumilio RNA recognition: the consequence of promiscuity. Structure. 2011 Mar 09; 19(3):277-9. PMID: 21397178.
      Citations: 2     Fields:    
    30. Zearfoss NR, Clingman CC, Farley BM, McCoig LM, Ryder SP. Quaking regulates Hnrnpa1 expression through its 3' UTR in oligodendrocyte precursor cells. PLoS Genet. 2011 Jan 06; 7(1):e1001269. PMID: 21253564.
      Citations: 34     Fields:    Translation:HumansAnimalsCells
    31. Wright JE, Gaidatzis D, Senften M, Farley BM, Westhof E, Ryder SP, Ciosk R. A quantitative RNA code for mRNA target selection by the germline fate determinant GLD-1. EMBO J. 2011 Feb 02; 30(3):533-45. PMID: 21169991.
      Citations: 58     Fields:    Translation:AnimalsCells
    32. Pagano JM, Clingman CC, Ryder SP. Quantitative approaches to monitor protein-nucleic acid interactions using fluorescent probes. RNA. 2011 Jan; 17(1):14-20. PMID: 21098142.
      Citations: 61     Fields:    Translation:HumansAnimalsCells
    33. Ryder SP. Hidden ribozymes in eukaryotic genome sequence. F1000 Biol Rep. 2010 Jul 14; 2. PMID: 20948783.
    34. Swamidass SJ, Bittker JA, Bodycombe NE, Ryder SP, Clemons PA. An economic framework to prioritize confirmatory tests after a high-throughput screen. J Biomol Screen. 2010 Jul; 15(6):680-6. PMID: 20547534.
      Citations: 7     Fields:    Translation:Humans
    35. Kaymak E, Wee LM, Ryder SP. Structure and function of nematode RNA-binding proteins. Curr Opin Struct Biol. 2010 Jun; 20(3):305-12. PMID: 20418095.
      Citations: 11     Fields:    Translation:HumansAnimalsCells
    36. Ryder SP, Massi F. Insights into the structural basis of RNA recognition by STAR domain proteins. Adv Exp Med Biol. 2010; 693:37-53. PMID: 21189684.
      Citations: 6     Fields:    Translation:HumansAnimalsCells
    37. Pagano JM, Farley BM, Essien KI, Ryder SP. RNA recognition by the embryonic cell fate determinant and germline totipotency factor MEX-3. Proc Natl Acad Sci U S A. 2009 Dec 01; 106(48):20252-7. PMID: 19915141.
      Citations: 42     Fields:    Translation:AnimalsCells
    38. Furgason ML, MacDonald C, Shanks SG, Ryder SP, Bryant NJ, Munson M. The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Proc Natl Acad Sci U S A. 2009 Aug 25; 106(34):14303-8. PMID: 19667197.
      Citations: 37     Fields:    Translation:AnimalsCells
    39. Farley BM, Pagano JM, Ryder SP. RNA target specificity of the embryonic cell fate determinant POS-1. RNA. 2008 Dec; 14(12):2685-97. PMID: 18952820.
      Citations: 33     Fields:    Translation:AnimalsCells
    40. Zearfoss NR, Farley BM, Ryder SP. Post-transcriptional regulation of myelin formation. Biochim Biophys Acta. 2008 Aug; 1779(8):486-94. PMID: 18590840.
      Citations: 10     Fields:    Translation:AnimalsCells
    41. Farley BM, Ryder SP. Regulation of maternal mRNAs in early development. Crit Rev Biochem Mol Biol. 2008 Mar-Apr; 43(2):135-62. PMID: 18365862.
      Citations: 27     Fields:    Translation:AnimalsCells
    42. Recht MI, Ryder SP, Williamson JR. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry. Methods Mol Biol. 2008; 488:117-27. PMID: 18982287.
      Citations: 3     Fields:    Translation:Cells
    43. Ryder SP, Recht MI, Williamson JR. Quantitative analysis of protein-RNA interactions by gel mobility shift. Methods Mol Biol. 2008; 488:99-115. PMID: 18982286.
      Citations: 104     Fields:    Translation:Cells
    44. Pagano JM, Farley BM, McCoig LM, Ryder SP. Molecular basis of RNA recognition by the embryonic polarity determinant MEX-5. J Biol Chem. 2007 Mar 23; 282(12):8883-94. PMID: 17264081.
      Citations: 63     Fields:    Translation:AnimalsCells
    45. Ryder SP. Oskar gains weight. Nat Struct Mol Biol. 2006 Apr; 13(4):297-9. PMID: 16715044.
      Citations:    Fields:    Translation:AnimalsCells
    46. Ryder SP, Williamson JR. Specificity of the STAR/GSG domain protein Qk1: implications for the regulation of myelination. RNA. 2004 Sep; 10(9):1449-58. PMID: 15273320.
      Citations: 49     Fields:    Translation:AnimalsCells
    47. Ryder SP, Frater LA, Abramovitz DL, Goodwin EB, Williamson JR. RNA target specificity of the STAR/GSG domain post-transcriptional regulatory protein GLD-1. Nat Struct Mol Biol. 2004 Jan; 11(1):20-8. PMID: 14718919.
      Citations: 57     Fields:    Translation:AnimalsCells
    48. Strobel SA, Jones FD, Oyelere AK, Ryder SP. Biochemical detection of adenosine and cytidine ionization within RNA by interference analysis. Nucleic Acids Res Suppl. 2003; (3):229-30. PMID: 14510464.
      Citations: 1     Fields:    
    49. Ryder SP, Strobel SA. Comparative analysis of hairpin ribozyme structures and interference data. Nucleic Acids Res. 2002 Mar 15; 30(6):1287-91. PMID: 11884625.
      Citations: 10     Fields:    Translation:Cells
    50. Jones FD, Ryder SP, Strobel SA. An efficient ligation reaction promoted by a Varkud Satellite ribozyme with extended 5'- and 3'-termini. Nucleic Acids Res. 2001 Dec 15; 29(24):5115-20. PMID: 11812844.
      Citations: 17     Fields:    Translation:AnimalsCells
    51. Ryder SP, Oyelere AK, Padilla JL, Klostermeier D, Millar DP, Strobel SA. Investigation of adenosine base ionization in the hairpin ribozyme by nucleotide analog interference mapping. RNA. 2001 Oct; 7(10):1454-63. PMID: 11680850.
      Citations: 22     Fields:    Translation:Cells
    52. Strobel SA, Ryder SP. Biological catalysis. The hairpin's turn. Nature. 2001 Apr 12; 410(6830):761-3. PMID: 11298426.
      Citations:    Fields:    Translation:Cells
    53. Ryder SP, Ortoleva-Donnelly L, Kosek AB, Strobel SA. Chemical probing of RNA by nucleotide analog interference mapping. Methods Enzymol. 2000; 317:92-109. PMID: 10829274.
      Citations: 18     Fields:    Translation:Cells
    54. Hess JA, Buchanan FG, Ryder S, Exton JH. Altered activation of phospholipase D by lysophosphatidic acid and endothelin-1 in mouse embryo fibroblasts lacking phospholipase C-gamma1. Cell Signal. 2000 Jan; 12(1):37-45. PMID: 10676846.
      Citations:    Fields:    Translation:AnimalsCells
    55. Ryder SP, Strobel SA. Nucleotide analog interference mapping of the hairpin ribozyme: implications for secondary and tertiary structure formation. J Mol Biol. 1999 Aug 13; 291(2):295-311. PMID: 10438622.
      Citations: 14     Fields:    Translation:Cells
    56. Ryder SP, Strobel SA. Nucleotide analog interference mapping. Methods. 1999 May; 18(1):38-50. PMID: 10208815.
      Citations: 27     Fields:    Translation:Cells
    57. Szewczak AA, Ortoleva-Donnelly L, Ryder SP, Moncoeur E, Strobel SA. A minor groove RNA triple helix within the catalytic core of a group I intron. Nat Struct Biol. 1998 Dec; 5(12):1037-42. PMID: 9846872.
      Citations: 22     Fields:    Translation:AnimalsCells
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