Header Logo

Daniel N Bolon PhD

InstitutionUMass Chan Medical School
DepartmentBiochemistry and Molecular Biotechnology
AddressUMass Chan Medical School
364 Plantation Street LRB
Worcester MA 01605
vCardDownload vCard
    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Biotechnology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentBioinformatics and Computational Biology

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentPostbaccalaureate Research Education Program

    InstitutionUMass Chan Programs, Centers and Institutes
    DepartmentBioinformatics and Integrative Biology

    Collapse Biography 
    Collapse education and training
    Duke University, Durham, NC, United StatesBSBiology
    California Institute of Technology, Pasadena, CA, United StatesPHDBiochemistry & Molecular Bio

    Collapse Overview 
    Collapse overview

    Academic Background

    Dan Bolon majored in Biology at Duke University (B.S., 1997). For Dan’s graduate work, he studied computational enzyme design with Steve Mayo at the California Institute of Technology (Ph.D. in Biochemistry and Molecular Biophysics, 2002). From 2002-2005, he trained as a postdoc with Bob Sauer in the Biology Department at the Massachusetts Institute of Technology using a variety of biochemical and biophysical techniques including X-ray crystallography, fluorescence, analytical ultracentrifugation, and protein engineering to study AAA+ proteases. Dan was awarded a NIH fellowship to support his postdoctoral studies (2004-2005). Other interests include mountain biking and baseball. Dan joined the faculty in Biochemistry and Molecular Pharmacology in September, 2005.

    Molecular mechanisms of adaptation in biology and diseasePhoto: Dan Bolon

    The ability of biological systems to adapt to new conditions rapidly is profoundly important because natural environments are continually changing. Thus, the ability of an organism to prosper is directly related to its ability to adapt. Adaptation is particularly important in human diseases including cancer and infection by viruses or bacteria. For example, the development of cancer involves adaptive changes within the cancer cells that bypass normal growth regulation. With bacterial and viral infections the severity of the outcome depends on the adaptive potential of the host defense systems relative to the pathogen. In the Bolon lab we are broadly interested in the molecular mechanisms of adaptation because of their central role in both biology and disease.

    Exploring the limits of adaptation by illuminating fitness landscapes

    Over time scales that span generations, adaptation is mediated by genetic variation. For example, the application of anti-viral drugs leads to strong selective pressure for drug-resistant mutations. Similarly, the evolution of all organisms is influenced by mutations that provide selective advantages within a specific environment. In natural systems, genetic variation is generated stochastically and thus represents a random walk through fitness space. Fitness space provides fundamental limits on the process of adaptation. To explore these fundamental biological constraints, we developed an experimental approach to measure and define the observe the fitness landscape of all possible point mutations for a gene. By combining saturation mutagenesis with growth competitions monitored by deep sequencing, we measure the fitness effects of thousands of different point mutations in parallel. We term this approach EMPIRIC (Exceedingly Meticulous and Parallel Investigation of Randomized Individual Codons). We are applying this approach to study many different fast growing biological entities including yeast, bacteria, cancer cells and viruses. This approach will provide both fundamental insights into selection pressure and valuable routes to improved therapeutics (i.e., by identifying sites in drug targets that cannot be mutated without impairing the function of the host cell and hence should be refractory to the development of drug resistance).

    Molecular mechanism of the Hsp90 chaperone

    The ability of organisms to respond to its environment on time-scales that shorter than a generation depends upon sensing the environment. Hsp90 is an essential protein that mediates these sensing processes because it is required for the maturation of many signal transduction proteins. Because Hsp90 substrates are mutated in many different forms of cancer, Hsp90 has emerged as a promising target for drugs to treat a broad spectrum of cancer. Hsp90 is clearly involved in many different essential processes in both healthy and diseased cells. However, how Hsp90 affects these processes is poorly understood. A major goal of our research is to elucidate the molecular mechanism of Hsp90 that orchestrates the dynamic assembly of Hsp90/co-chaperone/substrate complexes and the maturation of signal transduction clients to their active conformation. To probe the physical mechanism of this dynamic protein system we utilize biophysical, biochemical and genetic approaches to dissect the conformation and protein-protein interactions of Hsp90 during substrate maturation. The goal of this work is to delineate the physical mechanism by which Hsp90 matures substrates including those involved in cancer progression.

    Collapse Rotation Projects

    Potential Rotation Projects

    Our laboratory combines genetic and biochemical approaches to investigate the molecular underpinnings of adaptation. Potential rotation projects are available in two general (and partially overlapping) areas: exploring fitness landscapes through systematic approaches and investigating the molecular mechanism of the Hsp90 chaperone in signal transduction. Please contact the lab to discuss specific rotation projects in more detail.

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Flynn JM, Joyce ME, Bolon DNA. Dominant negative mutations in yeast Hsp90 reveal triage decision mechanism targeting client proteins for degradation. bioRxiv. 2024 Jan 02. PMID: 38260708.
    2. Padhy AA, Mavor D, Sahoo S, Bolon DNA, Mishra P. Systematic profiling of dominant ubiquitin variants reveals key functional nodes contributing to evolutionary selection. Cell Rep. 2023 09 26; 42(9):113064. PMID: 37656625.
      Citations: 1     Fields:    Translation:Animals
    3. Flynn JM, Huang QYJ, Zvornicanin SN, Schneider-Nachum G, Shaqra AM, Yilmaz NK, Moquin SA, Dovala D, Schiffer CA, Bolon DNA. Systematic Analyses of the Resistance Potential of Drugs Targeting SARS-CoV-2 Main Protease. ACS Infect Dis. 2023 07 14; 9(7):1372-1386. PMID: 37390404.
      Citations: 1     Fields:    Translation:HumansCells
    4. Mathy CJP, Mishra P, Flynn JM, Perica T, Mavor D, Bolon DNA, Kortemme T. A complete allosteric map of a GTPase switch in its native cellular network. Cell Syst. 2023 03 15; 14(3):237-246.e7. PMID: 36801015.
      Citations: 5     Fields:    Translation:Cells
    5. Flynn J, Samant N, Schneider-Nachum G, Tenzin T, Bolon DNA. Mutational fitness landscape and drug resistance. Curr Opin Struct Biol. 2023 02; 78:102525. PMID: 36621152.
      Citations:    Fields:    
    6. Samant N, Nachum G, Tsepal T, Bolon DNA. Sequence dependencies and biophysical features both govern cleavage of diverse cut-sites by HIV protease. Protein Sci. 2022 07; 31(7):e4366. PMID: 35762719.
      Citations:    Fields:    Translation:Cells
    7. Shaqra AM, Zvornicanin SN, Huang QYJ, Lockbaum GJ, Knapp M, Tandeske L, Bakan DT, Flynn J, Bolon DNA, Moquin S, Dovala D, Kurt Yilmaz N, Schiffer CA. Defining the substrate envelope of SARS-CoV-2 main protease to predict and avoid drug resistance. Nat Commun. 2022 06 21; 13(1):3556. PMID: 35729165.
      Citations: 24     Fields:    Translation:HumansCellsPHPublic Health
    8. Flynn JM, Samant N, Schneider-Nachum G, Barkan DT, Yilmaz NK, Schiffer CA, Moquin SA, Dovala D, Bolon DNA. Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanisms. Elife. 2022 06 20; 11. PMID: 35723575.
      Citations: 26     Fields:    Translation:HumansAnimalsCells
    9. Huang QJ, Song K, Xu C, Bolon DNA, Wang JP, Finberg RW, Schiffer CA, Somasundaran M. Quantitative structural analysis of influenza virus by cryo-electron tomography and convolutional neural networks. Structure. 2022 05 05; 30(5):777-786.e3. PMID: 35290796.
      Citations: 3     Fields:    Translation:HumansCells
    10. Jiang L, Samant N, Liu P, Somasundaran M, Jensen JD, Marasco WA, Kowalik TF, Schiffer CA, Finberg RW, Wang JP, Bolon DNA. Identification of a Permissive Secondary Mutation That Restores the Enzymatic Activity of Oseltamivir Resistance Mutation H275Y. J Virol. 2022 03 23; 96(6):e0198221. PMID: 35045267.
      Citations:    Fields:    Translation:HumansCells
    11. Schneider-Nachum G, Flynn J, Mavor D, Schiffer CA, Bolon DNA. Analyses of HIV proteases variants at the threshold of viability reveals relationships between processing efficiency and fitness. Virus Evol. 2021 Sep; 7(2):veab103. PMID: 35299788.
    12. Cote-Hammarlof PA, Fragata I, Flynn J, Mavor D, Zeldovich KB, Bank C, Bolon DNA. The Adaptive Potential of the Middle Domain of Yeast Hsp90. Mol Biol Evol. 2021 01 23; 38(2):368-379. PMID: 32871012.
      Citations: 3     Fields:    Translation:Animals
    13. Flynn JM, Rossouw A, Cote-Hammarlof P, Fragata I, Mavor D, Hollins C, Bank C, Bolon DN. Comprehensive fitness maps of Hsp90 show widespread environmental dependence. Elife. 2020 03 04; 9. PMID: 32129763.
      Citations: 26     Fields:    Translation:AnimalsCells
    14. Henes M, Kosovrasti K, Lockbaum GJ, Leidner F, Nachum GS, Nalivaika EA, Bolon DNA, Kurt Yilmaz N, Schiffer CA, Whitfield TW. Molecular Determinants of Epistasis in HIV-1 Protease: Elucidating the Interdependence of L89V and L90M Mutations in Resistance. Biochemistry. 2019 09 03; 58(35):3711-3726. PMID: 31386353.
      Citations: 10     Fields:    Translation:HumansCells
    15. Henes M, Lockbaum GJ, Kosovrasti K, Leidner F, Nachum GS, Nalivaika EA, Lee SK, Spielvogel E, Zhou S, Swanstrom R, Bolon DNA, Kurt Yilmaz N, Schiffer CA. Picomolar to Micromolar: Elucidating the Role of Distal Mutations in HIV-1 Protease in Conferring Drug Resistance. ACS Chem Biol. 2019 11 15; 14(11):2441-2452. PMID: 31361460.
      Citations: 19     Fields:    Translation:HumansCells
    16. Lockbaum GJ, Leidner F, Rusere LN, Henes M, Kosovrasti K, Nachum GS, Nalivaika EA, Bolon DNA, Ali A, Kurt Yilmaz N, Schiffer CA. Correction to Structural Adaptation of Darunavir Analogues against Primary Mutations in HIV-1 Protease. ACS Infect Dis. 2019 Jun 14; 5(6):1044. PMID: 30990653.
      Citations: 1     Fields:    
    17. Boucher JI, Whitfield TW, Dauphin A, Nachum G, Hollins C, Zeldovich KB, Swanstrom R, Schiffer CA, Luban J, Bolon DNA. Constrained Mutational Sampling of Amino Acids in HIV-1 Protease Evolution. Mol Biol Evol. 2019 04 01; 36(4):798-810. PMID: 30721995.
      Citations: 5     Fields:    Translation:Cells
    18. Boucher JI, Bolon DNA, Tawfik DS. Quantifying and understanding the fitness effects of protein mutations: Laboratory versus nature. Protein Sci. 2019 Mar; 28(3):671. PMID: 30747470.
      Citations: 1     Fields:    
    19. Prachanronarong KL, Canale AS, Liu P, Somasundaran M, Hou S, Poh YP, Han T, Zhu Q, Renzette N, Zeldovich KB, Kowalik TF, Kurt-Yilmaz N, Jensen JD, Bolon DNA, Marasco WA, Finberg RW, Schiffer CA, Wang JP. Mutations in Influenza A Virus Neuraminidase and Hemagglutinin Confer Resistance against a Broadly Neutralizing Hemagglutinin Stem Antibody. J Virol. 2019 01 15; 93(2). PMID: 30381484.
      Citations: 25     Fields:    Translation:AnimalsCells
    20. Mavor D, Barlow KA, Asarnow D, Birman Y, Britain D, Chen W, Green EM, Kenner LR, Mensa B, Morinishi LS, Nelson CA, Poss EM, Suresh P, Tian R, Arhar T, Ary BE, Bauer DP, Bergman ID, Brunetti RM, Chio CM, Dai SA, Dickinson MS, Elledge SK, Helsell CVM, Hendel NL, Kang E, Kern N, Khoroshkin MS, Kirkemo LL, Lewis GR, Lou K, Marin WM, Maxwell AM, McTigue PF, Myers-Turnbull D, Nagy TL, Natale AM, Oltion K, Pourmal S, Reder GK, Rettko NJ, Rohweder PJ, Schwarz DMC, Tan SK, Thomas PV, Tibble RW, Town JP, Tsai MK, Ugur FS, Wassarman DR, Wolff AM, Wu TS, Bogdanoff D, Li J, Thorn KS, O'Conch?ir S, Swaney DL, Chow ED, Madhani HD, Redding S, Bolon DN, Kortemme T, DeRisi JL, Kampmann M, Fraser JS. Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance. Biol Open. 2018 Jul 23; 7(7). PMID: 30037883.
      Citations: 12     Fields:    
    21. Starr TN, Flynn JM, Mishra P, Bolon DNA, Thornton JW. Pervasive contingency and entrenchment in a billion years of Hsp90 evolution. Proc Natl Acad Sci U S A. 2018 04 24; 115(17):4453-4458. PMID: 29626131.
      Citations: 38     Fields:    Translation:AnimalsCells
    22. Canale AS, Venev SV, Whitfield TW, Caffrey DR, Marasco WA, Schiffer CA, Kowalik TF, Jensen JD, Finberg RW, Zeldovich KB, Wang JP, Bolon DNA. Synonymous Mutations at the Beginning of the Influenza A Virus Hemagglutinin Gene Impact Experimental Fitness. J Mol Biol. 2018 04 13; 430(8):1098-1115. PMID: 29466705.
      Citations: 12     Fields:    Translation:HumansAnimalsCells
    23. Canale AS, Cote-Hammarlof PA, Flynn JM, Bolon DN. Evolutionary mechanisms studied through protein fitness landscapes. Curr Opin Struct Biol. 2018 02; 48:141-148. PMID: 29351890.
      Citations: 18     Fields:    Translation:HumansCells
    24. Ma L, Boucher JI, Paulsen J, Matuszewski S, Eide CA, Ou J, Eickelberg G, Press RD, Zhu LJ, Druker BJ, Branford S, Wolfe SA, Jensen JD, Schiffer CA, Green MR, Bolon DN. CRISPR-Cas9-mediated saturated mutagenesis screen predicts clinical drug resistance with improved accuracy. Proc Natl Acad Sci U S A. 2017 10 31; 114(44):11751-11756. PMID: 29078326.
      Citations: 32     Fields:    Translation:AnimalsCells
    25. Ichikawa Y, Connelly CF, Appleboim A, Miller TC, Jacobi H, Abshiru NA, Chou HJ, Chen Y, Sharma U, Zheng Y, Thomas PM, Chen HV, Bajaj V, M?ller CW, Kelleher NL, Friedman N, Bolon DN, Rando OJ, Kaufman PD. A synthetic biology approach to probing nucleosome symmetry. Elife. 2017 09 12; 6. PMID: 28895528.
      Citations: 11     Fields:    Translation:AnimalsCells
    26. Ormond L, Liu P, Matuszewski S, Renzette N, Bank C, Zeldovich K, Bolon DN, Kowalik TF, Finberg RW, Jensen JD, Wang JP. The Combined Effect of Oseltamivir and Favipiravir on Influenza A Virus Evolution. Genome Biol Evol. 2017 07 01; 9(7):1913-1924. PMID: 28854600.
      Citations: 16     Fields:    Translation:AnimalsCells
    27. Van Oosten-Hawle P, Bolon DN, LaPointe P. The diverse roles of Hsp90 and where to find them. Nat Struct Mol Biol. 2017 01 05; 24(1):1-4. PMID: 28054566.
      Citations: 1     Fields:    Translation:HumansAnimalsCells
    28. Prachanronarong KL, ?zen A, Thayer KM, Yilmaz LS, Zeldovich KB, Bolon DN, Kowalik TF, Jensen JD, Finberg RW, Wang JP, Kurt-Yilmaz N, Schiffer CA. Molecular Basis for Differential Patterns of Drug Resistance in Influenza N1 and N2 Neuraminidase. J Chem Theory Comput. 2016 Dec 13; 12(12):6098-6108. PMID: 27951676.
      Citations: 10     Fields:    Translation:HumansCells
    29. Duenas-Decamp M, Jiang L, Bolon D, Clapham PR. Saturation Mutagenesis of the HIV-1 Envelope CD4 Binding Loop Reveals Residues Controlling Distinct Trimer Conformations. PLoS Pathog. 2016 Nov; 12(11):e1005988. PMID: 27820858.
      Citations: 14     Fields:    Translation:HumansCells
    30. Bank C, Renzette N, Liu P, Matuszewski S, Shim H, Foll M, Bolon DN, Zeldovich KB, Kowalik TF, Finberg RW, Wang JP, Jensen JD. An experimental evaluation of drug-induced mutational meltdown as an antiviral treatment strategy. Evolution. 2016 11; 70(11):2470-2484. PMID: 27566611.
      Citations: 21     Fields:    Translation:AnimalsCells
    31. Bolon DN, Baker D, Tawfik DS. Editorial. Protein Sci. 2016 07; 25(7):1164-7. PMID: 27214768.
      Citations: 1     Fields:    Translation:Cells
    32. Mavor D, Barlow K, Thompson S, Barad BA, Bonny AR, Cario CL, Gaskins G, Liu Z, Deming L, Axen SD, Caceres E, Chen W, Cuesta A, Gate RE, Green EM, Hulce KR, Ji W, Kenner LR, Mensa B, Morinishi LS, Moss SM, Mravic M, Muir RK, Niekamp S, Nnadi CI, Palovcak E, Poss EM, Ross TD, Salcedo EC, See SK, Subramaniam M, Wong AW, Li J, Thorn KS, Conch?ir S?, Roscoe BP, Chow ED, DeRisi JL, Kortemme T, Bolon DN, Fraser JS. Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting. Elife. 2016 04 25; 5. PMID: 27111525.
      Citations: 38     Fields:    Translation:HumansAnimals
    33. Mishra P, Flynn JM, Starr TN, Bolon DNA. Systematic Mutant Analyses Elucidate General and Client-Specific Aspects of Hsp90 Function. Cell Rep. 2016 Apr 19; 15(3):588-598. PMID: 27068472.
      Citations: 39     Fields:    Translation:AnimalsCells
    34. Boucher JI, Bolon DN, Tawfik DS. Quantifying and understanding the fitness effects of protein mutations: Laboratory versus nature. Protein Sci. 2016 07; 25(7):1219-26. PMID: 27010590.
      Citations: 43     Fields:    Translation:Humans
    35. Jiang L, Liu P, Bank C, Renzette N, Prachanronarong K, Yilmaz LS, Caffrey DR, Zeldovich KB, Schiffer CA, Kowalik TF, Jensen JD, Finberg RW, Wang JP, Bolon DNA. A Balance between Inhibitor Binding and Substrate Processing Confers Influenza Drug Resistance. J Mol Biol. 2016 Feb 13; 428(3):538-553. PMID: 26656922.
      Citations: 20     Fields:    Translation:HumansAnimalsCells
    36. Flynn JM, Mishra P, Bolon DN. Mechanistic Asymmetry in Hsp90 Dimers. J Mol Biol. 2015 Sep 11; 427(18):2904-11. PMID: 25843003.
      Citations: 11     Fields:    Translation:HumansCells
    37. Zeldovich KB, Liu P, Renzette N, Foll M, Pham ST, Venev SV, Gallagher GR, Bolon DN, Kurt-Jones EA, Jensen JD, Caffrey DR, Schiffer CA, Kowalik TF, Wang JP, Finberg RW. Positive Selection Drives Preferred Segment Combinations during Influenza Virus Reassortment. Mol Biol Evol. 2015 Jun; 32(6):1519-32. PMID: 25713211.
      Citations: 10     Fields:    Translation:AnimalsCells
    38. Bank C, Hietpas RT, Jensen JD, Bolon DN. A systematic survey of an intragenic epistatic landscape. Mol Biol Evol. 2015 Jan; 32(1):229-38. PMID: 25371431.
      Citations: 75     Fields:    Translation:AnimalsCells
    39. Boucher JI, Cote P, Flynn J, Jiang L, Laban A, Mishra P, Roscoe BP, Bolon DN. Viewing protein fitness landscapes through a next-gen lens. Genetics. 2014 Oct; 198(2):461-71. PMID: 25316787.
      Citations: 31     Fields:    Translation:HumansAnimalsCells
    40. Roscoe BP, Bolon DN. Systematic exploration of ubiquitin sequence, E1 activation efficiency, and experimental fitness in yeast. J Mol Biol. 2014 Jul 29; 426(15):2854-70. PMID: 24862281.
      Citations: 25     Fields:    Translation:AnimalsCells
    41. Foll M, Poh YP, Renzette N, Ferrer-Admetlla A, Bank C, Shim H, Malaspinas AS, Ewing G, Liu P, Wegmann D, Caffrey DR, Zeldovich KB, Bolon DN, Wang JP, Kowalik TF, Schiffer CA, Finberg RW, Jensen JD. Influenza virus drug resistance: a time-sampled population genetics perspective. PLoS Genet. 2014 Feb; 10(2):e1004185. PMID: 24586206.
      Citations: 70     Fields:    Translation:HumansCells
    42. Mishra P, Bolon DN. Designed Hsp90 heterodimers reveal an asymmetric ATPase-driven mechanism in vivo. Mol Cell. 2014 Jan 23; 53(2):344-50. PMID: 24462207.
      Citations: 23     Fields:    Translation:AnimalsCells
    43. Bank C, Hietpas RT, Wong A, Bolon DN, Jensen JD. A bayesian MCMC approach to assess the complete distribution of fitness effects of new mutations: uncovering the potential for adaptive walks in challenging environments. Genetics. 2014 Mar; 196(3):841-52. PMID: 24398421.
      Citations: 52     Fields:    Translation:Animals
    44. Lee SY, Pullen L, Virgil DJ, Casta?eda CA, Abeykoon D, Bolon DN, Fushman D. Alanine scan of core positions in ubiquitin reveals links between dynamics, stability, and function. J Mol Biol. 2014 Apr 03; 426(7):1377-89. PMID: 24361330.
      Citations: 13     Fields:    Translation:Animals
    45. Renzette N, Caffrey DR, Zeldovich KB, Liu P, Gallagher GR, Aiello D, Porter AJ, Kurt-Jones EA, Bolon DN, Poh YP, Jensen JD, Schiffer CA, Kowalik TF, Finberg RW, Wang JP. Evolution of the influenza A virus genome during development of oseltamivir resistance in vitro. J Virol. 2014 Jan; 88(1):272-81. PMID: 24155392.
      Citations: 27     Fields:    Translation:AnimalsCells
    46. Wagenaar TR, Ma L, Roscoe B, Park SM, Bolon DN, Green MR. Resistance to vemurafenib resulting from a novel mutation in the BRAFV600E kinase domain. Pigment Cell Melanoma Res. 2014 Jan; 27(1):124-33. PMID: 24112705.
      Citations: 26     Fields:    Translation:HumansAnimalsCells
    47. Hietpas RT, Bank C, Jensen JD, Bolon DNA. Shifting fitness landscapes in response to altered environments. Evolution. 2013 Dec; 67(12):3512-22. PMID: 24299404.
      Citations: 64     Fields:    Translation:Animals
    48. Beebe K, Mollapour M, Scroggins B, Prodromou C, Xu W, Tokita M, Taldone T, Pullen L, Zierer BK, Lee MJ, Trepel J, Buchner J, Bolon D, Chiosis G, Neckers L. Posttranslational modification and conformational state of heat shock protein 90 differentially affect binding of chemically diverse small molecule inhibitors. Oncotarget. 2013 Jul; 4(7):1065-74. PMID: 23867252.
      Citations: 40     Fields:    Translation:HumansCells
    49. Jiang L, Mishra P, Hietpas RT, Zeldovich KB, Bolon DN. Latent effects of Hsp90 mutants revealed at reduced expression levels. PLoS Genet. 2013 Jun; 9(6):e1003600. PMID: 23825969.
      Citations: 55     Fields:    Translation:AnimalsCells
    50. Roscoe BP, Thayer KM, Zeldovich KB, Fushman D, Bolon DN. Analyses of the effects of all ubiquitin point mutants on yeast growth rate. J Mol Biol. 2013 Apr 26; 425(8):1363-77. PMID: 23376099.
      Citations: 118     Fields:    Translation:Animals
    51. Hietpas R, Roscoe B, Jiang L, Bolon DN. Fitness analyses of all possible point mutations for regions of genes in yeast. Nat Protoc. 2012 Jun 21; 7(7):1382-96. PMID: 22722372.
      Citations: 42     Fields:    Translation:Animals
    52. Pursell NW, Mishra P, Bolon DN. Solubility-promoting function of Hsp90 contributes to client maturation and robust cell growth. Eukaryot Cell. 2012 Aug; 11(8):1033-41. PMID: 22660624.
      Citations: 8     Fields:    Translation:AnimalsCells
    53. Mittal S, Cai Y, Nalam MN, Bolon DN, Schiffer CA. Hydrophobic core flexibility modulates enzyme activity in HIV-1 protease. J Am Chem Soc. 2012 Mar 07; 134(9):4163-8. PMID: 22295904.
      Citations: 28     Fields:    Translation:Cells
    54. Bolon DN. Bound for observation. J Mol Biol. 2012 Jan 06; 415(1):1-2. PMID: 22094315.
      Citations:    Fields:    Translation:Humans
    55. Hietpas RT, Jensen JD, Bolon DN. Experimental illumination of a fitness landscape. Proc Natl Acad Sci U S A. 2011 May 10; 108(19):7896-901. PMID: 21464309.
      Citations: 168     Fields:    Translation:Animals
    56. Pullen L, Bolon DN. Enforced N-domain proximity stimulates Hsp90 ATPase activity and is compatible with function in vivo. J Biol Chem. 2011 Apr 01; 286(13):11091-8. PMID: 21278257.
      Citations: 21     Fields:    Translation:HumansCells
    57. Wayne N, Mishra P, Bolon DN. Hsp90 and client protein maturation. Methods Mol Biol. 2011; 787:33-44. PMID: 21898225.
      Citations: 33     Fields:    Translation:HumansAnimalsCells
    58. Wayne N, Bolon DN. Charge-rich regions modulate the anti-aggregation activity of Hsp90. J Mol Biol. 2010 Sep 03; 401(5):931-9. PMID: 20615417.
      Citations: 22     Fields:    Translation:Cells
    59. Wayne N, Lai Y, Pullen L, Bolon DN. Modular control of cross-oligomerization: analysis of superstabilized Hsp90 homodimers in vivo. J Biol Chem. 2010 Jan 01; 285(1):234-41. PMID: 19906642.
      Citations: 11     Fields:    Translation:AnimalsCells
    60. Munson M, Bolon DN. Watching proteins in motion. Genome Biol. 2009; 10(10):316. PMID: 19863776.
      Citations:    Fields:    Translation:HumansAnimalsCells
    61. Haririnia A, Verma R, Purohit N, Twarog MZ, Deshaies RJ, Bolon D, Fushman D. Mutations in the hydrophobic core of ubiquitin differentially affect its recognition by receptor proteins. J Mol Biol. 2008 Jan 25; 375(4):979-96. PMID: 18054791.
      Citations: 25     Fields:    Translation:HumansCells
    62. Wayne N, Bolon DN. Dimerization of Hsp90 is required for in vivo function. Design and analysis of monomers and dimers. J Biol Chem. 2007 Nov 30; 282(48):35386-95. PMID: 17908693.
      Citations: 50     Fields:    Translation:HumansAnimalsCells
    63. McGinness KE, Bolon DN, Kaganovich M, Baker TA, Sauer RT. Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery. J Biol Chem. 2007 Apr 13; 282(15):11465-73. PMID: 17317664.
      Citations: 22     Fields:    Translation:Cells
    64. Bolon DN, Grant RA, Baker TA, Sauer RT. Specificity versus stability in computational protein design. Proc Natl Acad Sci U S A. 2005 Sep 06; 102(36):12724-9. PMID: 16129838.
      Citations: 63     Fields:    Translation:Cells
    65. Hersch GL, Burton RE, Bolon DN, Baker TA, Sauer RT. Asymmetric interactions of ATP with the AAA+ ClpX6 unfoldase: allosteric control of a protein machine. Cell. 2005 Jul 01; 121(7):1017-27. PMID: 15989952.
      Citations: 89     Fields:    Translation:Cells
    66. Bolon DN, Grant RA, Baker TA, Sauer RT. Nucleotide-dependent substrate handoff from the SspB adaptor to the AAA+ ClpXP protease. Mol Cell. 2004 Nov 05; 16(3):343-50. PMID: 15525508.
      Citations: 45     Fields:    Translation:Cells
    67. Sauer RT, Bolon DN, Burton BM, Burton RE, Flynn JM, Grant RA, Hersch GL, Joshi SA, Kenniston JA, Levchenko I, Neher SB, Oakes ES, Siddiqui SM, Wah DA, Baker TA. Sculpting the proteome with AAA(+) proteases and disassembly machines. Cell. 2004 Oct 01; 119(1):9-18. PMID: 15454077.
      Citations: 187     Fields:    Translation:HumansAnimalsCells
    68. Bolon DN, Wah DA, Hersch GL, Baker TA, Sauer RT. Bivalent tethering of SspB to ClpXP is required for efficient substrate delivery: a protein-design study. Mol Cell. 2004 Feb 13; 13(3):443-9. PMID: 14967151.
      Citations: 35     Fields:    Translation:Cells
    69. Wah DA, Levchenko I, Rieckhof GE, Bolon DN, Baker TA, Sauer RT. Flexible linkers leash the substrate binding domain of SspB to a peptide module that stabilizes delivery complexes with the AAA+ ClpXP protease. Mol Cell. 2003 Aug; 12(2):355-63. PMID: 14536075.
      Citations: 45     Fields:    Translation:Cells
    70. Bolon DN, Marcus JS, Ross SA, Mayo SL. Prudent modeling of core polar residues in computational protein design. J Mol Biol. 2003 Jun 06; 329(3):611-22. PMID: 12767838.
      Citations: 9     Fields:    Translation:Cells
    71. Bolon DN, Voigt CA, Mayo SL. De novo design of biocatalysts. Curr Opin Chem Biol. 2002 Apr; 6(2):125-9. PMID: 12038994.
      Citations: 30     Fields:    Translation:Cells
    72. Bolon DN, Mayo SL. Enzyme-like proteins by computational design. Proc Natl Acad Sci U S A. 2001 Dec 04; 98(25):14274-9. PMID: 11724958.
      Citations: 130     Fields:    Translation:Cells
    73. Ambrosino DM, Bolon D, Collard H, Van Etten R, Kanchana MV, Finberg RW. Effect of Haemophilus influenzae polysaccharide outer membrane protein complex conjugate vaccine on macrophages. J Immunol. 1992 Dec 15; 149(12):3978-83. PMID: 1460286.
      Citations: 6     Fields:    Translation:AnimalsCellsPHPublic Health
    Bolon's Networks
    Click the
    buttons for more information and interactive visualizations!
    Concepts (289)
    Co-Authors (19)
    Similar People (60)
    Same Department Expand Description
    Physical Neighbors