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N-ethylmaleimide (NEM) can significantly improve in situ hybridization results using 35S-labeled oligodeoxynucleotide or complementary RNA probes.

Zoeller RT, Fletcher DL, Butnariu O, Lowry CA, Moore FL. N-ethylmaleimide (NEM) can significantly improve in situ hybridization results using 35S-labeled oligodeoxynucleotide or complementary RNA probes. J Histochem Cytochem. 1997 Jul; 45(7):1035-41.

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