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Erik J Sontheimer PhD

TitleProfessor
InstitutionUniversity of Massachusetts Medical School
DepartmentRNA Therapeutics Institute
AddressUniversity of Massachusetts Medical School
368 Plantation Street, Sherman Center, AS5.2051
Worcester MA 01605
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    Other Positions
    InstitutionUMMS - School of Medicine
    DepartmentProgram in Molecular Medicine

    InstitutionUMMS - School of Medicine
    DepartmentRNA Therapeutics Institute

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentBiochemistry and Molecular Pharmacology

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentImmunology and Microbiology Program

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionUMMS - Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program


    Collapse Biography 
    Collapse education and training
    Pennsylvania State University, College Park, PA, United StatesBSMolecular & Cell Biology
    Yale University, New Haven, CT, United StatesPHDMolecular Biophysics & Biochem

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ. NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol. 2018 Dec 05; 19(1):214. PMID: 30518407.
      View in: PubMed
    2. Lee J, Mir A, Edraki A, Garcia B, Amrani N, Lou HE, Gainetdinov I, Pawluk A, Ibraheim R, Gao XD, Liu P, Davidson AR, Maxwell KL, Sontheimer EJ. Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins. MBio. 2018 Dec 04; 9(6). PMID: 30514786.
      View in: PubMed
    3. Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA. Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Nat Commun. 2018 Nov 19; 9(1):4856. PMID: 30451839.
      View in: PubMed
    4. Ibraheim R, Song CQ, Mir A, Amrani N, Xue W, Sontheimer EJ. All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo. Genome Biol. 2018 Sep 19; 19(1):137. PMID: 30231914.
      View in: PubMed
    5. Mir A, Alterman JF, Hassler MR, Debacker AJ, Hudgens E, Echeverria D, Brodsky MH, Khvorova A, Watts JK, Sontheimer EJ. Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing. Nat Commun. 2018 Jul 06; 9(1):2641. PMID: 29980686.
      View in: PubMed
    6. Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ. C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2. Nat Methods. 2018 May 07. PMID: 29735996.
      View in: PubMed
    7. Stone NP, Hilbert BJ, Hidalgo D, Halloran KT, Lee J, Sontheimer EJ, Kelch BA. A Hyperthermophilic Phage Decoration Protein Suggests Common Evolutionary Origin with Herpesvirus Triplex Proteins and an Anti-CRISPR Protein. Structure. 2018 Apr 26. PMID: 29779790.
      View in: PubMed
    8. Edraki A, Sontheimer EJ. CRISPRs from scratch. Nat Microbiol. 2018 Mar; 3(3):261-262. PMID: 29463924.
      View in: PubMed
    9. Mir A, Edraki A, Lee J, Sontheimer EJ. Type II-C CRISPR-Cas9 Biology, Mechanism, and Application. ACS Chem Biol. 2018 Feb 16; 13(2):357-365. PMID: 29202216.
      View in: PubMed
    10. Harrington LB, Doxzen KW, Ma E, Liu JJ, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA. A Broad-Spectrum Inhibitor of CRISPR-Cas9. Cell. 2017 Sep 07; 170(6):1224-1233.e15. PMID: 28844692.
      View in: PubMed
    11. Sontheimer EJ, Davidson AR. Inhibition of CRISPR-Cas systems by mobile genetic elements. Curr Opin Microbiol. 2017 Jun; 37:120-127. PMID: 28668720.
      View in: PubMed
    12. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017 Jun 14; 18(1):108. PMID: 28615073.
      View in: PubMed
    13. Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. Naturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15; 167(7):1829-1838.e9. PMID: 27984730.
      View in: PubMed
    14. Sontheimer EJ, Marraffini LA. RNA. CRISPR goes retro. Science. 2016 Feb 26; 351(6276):920-1. PMID: 26917756.
      View in: PubMed
    15. Sontheimer EJ, Wolfe SA. Cas9 gets a classmate. Nat Biotechnol. 2015 Dec 09; 33(12):1240-1241. PMID: 26650011.
      View in: PubMed
    16. Zhang Y, Rajan R, Seifert HS, Mondragón A, Sontheimer EJ. DNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15; 60(2):242-55. PMID: 26474066.
      View in: PubMed
    17. Wakefield N, Rajan R, Sontheimer EJ. Primary processing of CRISPR RNA by the endonuclease Cas6 in Staphylococcus epidermidis. FEBS Lett. 2015 Oct 07; 589(20 Pt B):3197-204. PMID: 26364721.
      View in: PubMed
    18. Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ, Weng Z, Gao G, Xue W. Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Hum Gene Ther. 2015 Jul; 26(7):432-42. PMID: 26086867.
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    19. Sontheimer EJ, Barrangou R. The Bacterial Origins of the CRISPR Genome-Editing Revolution. Hum Gene Ther. 2015 Jul; 26(7):413-24. PMID: 26078042.
      View in: PubMed
    20. Sontheimer E. Accelerating expansion. RNA. 2015 Apr; 21(4):510. PMID: 25780117.
      View in: PubMed
    21. Zhang Y, Sontheimer EJ. Structural biology. Cascading into focus. Science. 2014 Sep 19; 345(6203):1452-3. PMID: 25237089.
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    22. Hurtado S, Kim Guisbert KS, Sontheimer EJ. SPO24 is a transcriptionally dynamic, small ORF-encoding locus required for efficient sporulation in Saccharomyces cerevisiae. PLoS One. 2014; 9(8):e105058. PMID: 25127041.
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    23. Doudna JA, Sontheimer EJ. Methods in Enzymology. The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations. Preface. Methods Enzymol. 2014; 546:xix-xx. PMID: 25398356.
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    24. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Sep 24; 110(39):15644-9. PMID: 23940360.
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    25. Zhang Y, Heidrich N, Ampattu BJ, Gunderson CW, Seifert HS, Schoen C, Vogel J, Sontheimer EJ. Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis. Mol Cell. 2013 May 23; 50(4):488-503. PMID: 23706818.
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    26. Kim Guisbert KS, Sontheimer EJ. Quit stalling or you'll be silenced. Cell. 2013 Feb 28; 152(5):938-9. PMID: 23452843.
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    27. Sontheimer EJ. Small RNAs of opposite sign… but same absolute value. Cell. 2012 Dec 7; 151(6):1157-8. PMID: 23217700.
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    28. Kim Guisbert KS, Zhang Y, Flatow J, Hurtado S, Staley JP, Lin S, Sontheimer EJ. Meiosis-induced alterations in transcript architecture and noncoding RNA expression in S. cerevisiae. RNA. 2012 Jun; 18(6):1142-53. PMID: 22539527.
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    29. Gerbasi VR, Preall JB, Golden DE, Powell DW, Cummins TD, Sontheimer EJ. Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis. Proc Natl Acad Sci U S A. 2011 Feb 22; 108(8):3204-9. PMID: 21300896.
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    30. Sontheimer EJ, Marraffini LA. Microbiology: slicer for DNA. Nature. 2010 Nov 4; 468(7320):45-6. PMID: 21048757.
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    31. Gerbasi VR, Golden DE, Hurtado SB, Sontheimer EJ. Proteomics identification of Drosophila small interfering RNA-associated factors. Mol Cell Proteomics. 2010 Sep; 9(9):1866-72. PMID: 20472918.
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    32. Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet. 2010 Mar; 11(3):181-90. PMID: 20125085.
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    33. Marraffini LA, Sontheimer EJ. Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature. 2010 Jan 28; 463(7280):568-71. PMID: 20072129.
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    34. Lee YS, Pressman S, Andress AP, Kim K, White JL, Cassidy JJ, Li X, Lubell K, Lim DH, Cho IS, Nakahara K, Preall JB, Bellare P, Sontheimer EJ, Carthew RW. Silencing by small RNAs is linked to endosomal trafficking. Nat Cell Biol. 2009 Sep; 11(9):1150-6. PMID: 19684574.
      View in: PubMed
    35. Marraffini LA, Sontheimer EJ. Invasive DNA, chopped and in the CRISPR. Structure. 2009 Jun 10; 17(6):786-8. PMID: 19523896.
      View in: PubMed
    36. Carthew RW, Sontheimer EJ. Origins and Mechanisms of miRNAs and siRNAs. Cell. 2009 Feb 20; 136(4):642-55. PMID: 19239886.
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    37. Marraffini LA, Sontheimer EJ. CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA. Science. 2008 Dec 19; 322(5909):1843-5. PMID: 19095942.
      View in: PubMed
    38. Golden DE, Gerbasi VR, Sontheimer EJ. An inside job for siRNAs. Mol Cell. 2008 Aug 8; 31(3):309-12. PMID: 18691963.
      View in: PubMed
    39. Bellare P, Small EC, Huang X, Wohlschlegel JA, Staley JP, Sontheimer EJ. A role for ubiquitin in the spliceosome assembly pathway. Nat Struct Mol Biol. 2008 May; 15(5):444-51. PMID: 18425143.
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    40. Bellare P, Sontheimer EJ. A fork in the road for microRNAs. Nat Struct Mol Biol. 2007 Aug; 14(8):684-6. PMID: 17676029.
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    41. Preall JB, He Z, Gorra JM, Sontheimer EJ. Short interfering RNA strand selection is independent of dsRNA processing polarity during RNAi in Drosophila. Curr Biol. 2006 Mar 7; 16(5):530-5. PMID: 16527750.
      View in: PubMed
    42. Bellare P, Kutach AK, Rines AK, Guthrie C, Sontheimer EJ. Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. 2006 Feb; 12(2):292-302. PMID: 16428608.
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    43. Preall JB, Sontheimer EJ. RNAi: RISC gets loaded. Cell. 2005 Nov 18; 123(4):543-5. PMID: 16286001.
      View in: PubMed
    44. Pham JW, Sontheimer EJ. Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway. J Biol Chem. 2005 Nov 25; 280(47):39278-83. PMID: 16179342.
      View in: PubMed
    45. Pellino JL, Jaskiewicz L, Filipowicz W, Sontheimer EJ. ATP modulates siRNA interactions with an endogenous human Dicer complex. RNA. 2005 Nov; 11(11):1719-24. PMID: 16177131.
      View in: PubMed
    46. Sontheimer EJ, Carthew RW. Silence from within: endogenous siRNAs and miRNAs. Cell. 2005 Jul 15; 122(1):9-12. PMID: 16009127.
      View in: PubMed
    47. Sontheimer EJ. Assembly and function of RNA silencing complexes. Nat Rev Mol Cell Biol. 2005 Feb; 6(2):127-38. PMID: 15654322.
      View in: PubMed
    48. Pham JW, Sontheimer EJ. Separation of Drosophila RNA silencing complexes by native gel electrophoresis. Methods Mol Biol. 2005; 309:11-6. PMID: 15990394.
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    49. Sontheimer EJ, Carthew RW. Molecular biology. Argonaute journeys into the heart of RISC. Science. 2004 Sep 3; 305(5689):1409-10. PMID: 15353786.
      View in: PubMed
    50. He Z, Sontheimer EJ. "siRNAs and miRNAs": a meeting report on RNA silencing. RNA. 2004 Aug; 10(8):1165-73. PMID: 15272116.
      View in: PubMed
    51. Pham JW, Sontheimer EJ. The Making of an siRNA. Mol Cell. 2004 Jul 23; 15(2):163-4. PMID: 15260964.
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    52. Pham JW, Radhakrishnan I, Sontheimer EJ. Thermodynamic and structural characterization of 2'-nitrogen-modified RNA duplexes. Nucleic Acids Res. 2004; 32(11):3446-55. PMID: 15247335.
      View in: PubMed
    53. Pham JW, Pellino JL, Lee YS, Carthew RW, Sontheimer EJ. A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell. 2004 Apr 2; 117(1):83-94. PMID: 15066284.
      View in: PubMed
    54. Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ, Carthew RW. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell. 2004 Apr 2; 117(1):69-81. PMID: 15066283.
      View in: PubMed
    55. Pellino JL, Sontheimer EJ. R2D2 leads the silencing trigger to mRNA's death star. Cell. 2003 Oct 17; 115(2):132-3. PMID: 14567910.
      View in: PubMed
    56. Sontheimer EJ. The spliceosome shows its metal. Nat Struct Biol. 2001 Jan; 8(1):11-3. PMID: 11135658.
      View in: PubMed
    57. Gordon PM, Sontheimer EJ, Piccirilli JA. Kinetic characterization of the second step of group II intron splicing: role of metal ions and the cleavage site 2'-OH in catalysis. Biochemistry. 2000 Oct 24; 39(42):12939-52. PMID: 11041859.
      View in: PubMed
    58. Warnecke JM, Sontheimer EJ, Piccirilli JA, Hartmann RK. Active site constraints in the hydrolysis reaction catalyzed by bacterial RNase P: analysis of precursor tRNAs with a single 3'-S-phosphorothiolate internucleotide linkage. Nucleic Acids Res. 2000 Feb 1; 28(3):720-7. PMID: 10637323.
      View in: PubMed
    59. Gordon PM, Sontheimer EJ, Piccirilli JA. Metal ion catalysis during the exon-ligation step of nuclear pre-mRNA splicing: extending the parallels between the spliceosome and group II introns. RNA. 2000 Feb; 6(2):199-205. PMID: 10688359.
      View in: PubMed
    60. Sontheimer EJ, Gordon PM, Piccirilli JA. Metal ion catalysis during group II intron self-splicing: parallels with the spliceosome. Genes Dev. 1999 Jul 1; 13(13):1729-41. PMID: 10398685.
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    61. Sontheimer EJ. Bridging sulfur substitutions in the analysis of pre-mRNA splicing. Methods. 1999 May; 18(1):29-37. PMID: 10208814.
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    62. Sontheimer EJ, Sun S, Piccirilli JA. Metal ion catalysis during splicing of premessenger RNA. Nature. 1997 Aug 21; 388(6644):801-5. PMID: 9285595.
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    63. Sontheimer EJ. Site-specific RNA crosslinking with 4-thiouridine. Mol Biol Rep. 1994 Jul; 20(1):35-44. PMID: 7531281.
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    64. Sontheimer EJ, Steitz JA. The U5 and U6 small nuclear RNAs as active site components of the spliceosome. Science. 1993 Dec 24; 262(5142):1989-96. PMID: 8266094.
      View in: PubMed
    65. Cortes JJ, Sontheimer EJ, Seiwert SD, Steitz JA. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo. EMBO J. 1993 Dec 15; 12(13):5181-9. PMID: 8262061.
      View in: PubMed
    66. Wyatt JR, Sontheimer EJ, Steitz JA. Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. Genes Dev. 1992 Dec; 6(12B):2542-53. PMID: 1340469.
      View in: PubMed
    67. Gelpi C, Sontheimer EJ, Rodriguez-Sanchez JL. Autoantibodies against a serine tRNA-protein complex implicated in cotranslational selenocysteine insertion. Proc Natl Acad Sci U S A. 1992 Oct 15; 89(20):9739-43. PMID: 1409691.
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    68. Sontheimer EJ, Steitz JA. Three novel functional variants of human U5 small nuclear RNA. Mol Cell Biol. 1992 Feb; 12(2):734-46. PMID: 1310151.
      View in: PubMed
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