Two-Hybrid System Techniques

"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity.

MeSH information

Definition | Details | More General Concepts | Related Concepts | More Specific Concepts

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

Descriptor ID	D020798
MeSH Number(s)	E05.393.220.870 E05.601.690.650 E05.601.870
Concept/Terms	Two-Hybrid System Techniques Two-Hybrid System Techniques Technique, Two-Hybrid System Techniques, Two-Hybrid System Two Hybrid System Techniques Two-Hybrid System Technique Two-Hybrid Method Method, Two-Hybrid Methods, Two-Hybrid Two Hybrid Method Two-Hybrid Methods Two-Hybrid System Technics Technic, Two-Hybrid System Technics, Two-Hybrid System Two Hybrid System Technics Two-Hybrid System Technic Two-Hybrid Assay Assay, Two-Hybrid Assays, Two-Hybrid Two Hybrid Assay Two-Hybrid Assays One-Hybrid System Techniques One-Hybrid System Techniques One Hybrid System Techniques One-Hybrid System Technique Technique, One-Hybrid System Techniques, One-Hybrid System One-Hybrid System Technics One Hybrid System Technics One-Hybrid System Technic Technic, One-Hybrid System Technics, One-Hybrid System Reverse One-Hybrid System Techniques Reverse One-Hybrid System Techniques Reverse One Hybrid System Techniques Yeast Two-Hybrid System Techniques Yeast Two-Hybrid System Techniques Yeast Two Hybrid System Techniques Yeast Two-Hybrid System System, Yeast Two-Hybrid Systems, Yeast Two-Hybrid Two-Hybrid System, Yeast Two-Hybrid Systems, Yeast Yeast Two Hybrid System Yeast Two-Hybrid Systems Yeast Two-Hybrid Assay Assay, Yeast Two-Hybrid Assays, Yeast Two-Hybrid Two-Hybrid Assay, Yeast Two-Hybrid Assays, Yeast Yeast Two Hybrid Assay Yeast Two-Hybrid Assays Reverse Two-Hybrid System Techniques Reverse Two-Hybrid System Techniques Reverse Two Hybrid System Techniques Three-Hybrid System Techniques Three-Hybrid System Techniques Technique, Three-Hybrid System Techniques, Three-Hybrid System Three Hybrid System Techniques Three-Hybrid System Technique Tri-Hybrid System Techniques Technique, Tri-Hybrid System Techniques, Tri-Hybrid System Tri Hybrid System Techniques Tri-Hybrid System Technique Three-Hybrid System Technics Technic, Three-Hybrid System Technics, Three-Hybrid System Three Hybrid System Technics Three-Hybrid System Technic Yeast One-Hybrid System Techniques Yeast One-Hybrid System Techniques Yeast One Hybrid System Techniques Yeast Three-Hybrid System Techniques Yeast Three-Hybrid System Techniques Yeast Three Hybrid System Techniques Yeast Three-Hybrid System System, Yeast Three-Hybrid Systems, Yeast Three-Hybrid Three-Hybrid System, Yeast Three-Hybrid Systems, Yeast Yeast Three Hybrid System Yeast Three-Hybrid Systems Yeast Three-Hybrid Assay Assay, Yeast Three-Hybrid Assays, Yeast Three-Hybrid Three-Hybrid Assay, Yeast Three-Hybrid Assays, Yeast Yeast Three Hybrid Assay Yeast Three-Hybrid Assays n-Hybrid System Techniques n-Hybrid System Techniques System Technique, n-Hybrid System Techniques, n-Hybrid Technique, n-Hybrid System Techniques, n-Hybrid System n Hybrid System Techniques n-Hybrid System Technique Reverse Three-Hybrid System Techniques Reverse Three-Hybrid System Techniques Reverse Three Hybrid System Techniques

Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".

Analytical, Diagnostic and Therapeutic Techniques and Equipment [E]
Investigative Techniques [E05]
Genetic Techniques [E05.393]
Cloning, Molecular [E05.393.220]
Two-Hybrid System Techniques [E05.393.220.870]
Molecular Probe Techniques [E05.601]
Protein Interaction Mapping [E05.601.690]
Two-Hybrid System Techniques [E05.601.690.650]
Two-Hybrid System Techniques [E05.601.870]

Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".

publications

Timeline | Most Recent

This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications.

Bar chart showing 58 publications over 18 distinct years, with a maximum of 7 publications in 2004

To see the data from this visualization as text, click here.

Year	Major Topic	Minor Topic	Total
1995	0	1	1
2001	0	3	3
2002	0	4	4
2003	0	2	2
2004	0	7	7
2005	1	4	5
2006	1	3	4
2007	1	3	4
2008	1	4	5
2009	0	3	3
2010	0	5	5
2011	3	2	5
2012	0	2	2
2013	0	2	2
2015	0	3	3
2016	0	1	1
2019	0	1	1
2020	0	1	1

Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.

Orr RG, Furt F, Warner EL, Agar EM, Garbarino JM, Cabral SE, Dubuke ML, Butt AM, Munson M, Vidali L. Rab-E and its interaction with myosin XI are essential for polarised cell growth. New Phytol. 2021 02; 229(4):1924-1936.

View in: PubMed
Wang LW, Nandadasa S, Annis DS, Dubail J, Mosher DF, Willard BB, Apte SS. A disintegrin-like and metalloproteinase domain with thrombospondin type 1 motif 9 (ADAMTS9) regulates fibronectin fibrillogenesis and turnover. J Biol Chem. 2019 06 21; 294(25):9924-9936.

View in: PubMed
Sparks EE, Drapek C, Gaudinier A, Li S, Ansariola M, Shen N, Hennacy JH, Zhang J, Turco G, Petricka JJ, Foret J, Hartemink AJ, Gord?n R, Megraw M, Brady SM, Benfey PN. Establishment of Expression in the SHORTROOT-SCARECROW Transcriptional Cascade through Opposing Activities of Both Activators and Repressors. Dev Cell. 2016 12 05; 39(5):585-596.

View in: PubMed
Ravindranath AK, Kaur S, Wernyj RP, Kumaran MN, Miletti-Gonzalez KE, Chan R, Lim E, Madura K, Rodriguez-Rodriguez L. CD44 promotes multi-drug resistance by protecting P-glycoprotein from FBXO21-mediated ubiquitination. Oncotarget. 2015 Sep 22; 6(28):26308-21.

View in: PubMed
Hallstrom KN, Srikanth CV, Agbor TA, Dumont CM, Peters KN, Paraoan L, Casanova JE, Boll EJ, McCormick BA. PERP, a host tetraspanning membrane protein, is required for Salmonella-induced inflammation. Cell Microbiol. 2015 Jun; 17(6):843-59.

View in: PubMed
Spinazzola JM, Smith TC, Liu M, Luna EJ, Barton ER. Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle. Hum Mol Genet. 2015 May 01; 24(9):2470-81.

View in: PubMed
de Souza EE, Hehnly H, Perez AM, Meirelles GV, Smetana JH, Doxsey S, Kobarg J. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle. 2015; 14(4):656-67.

View in: PubMed
de Souza EE, Meirelles GV, Godoy BB, Perez AM, Smetana JH, Doxsey SJ, McComb ME, Costello CE, Whelan SA, Kobarg J. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res. 2014 Sep 05; 13(9):4074-90.

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Nagarajan A, Dogra SK, Liu AY, Green MR, Wajapeyee N. PEA15 regulates the DNA damage-induced cell cycle checkpoint and oncogene-directed transformation. Mol Cell Biol. 2014 Jun; 34(12):2264-82.

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He F, Ganesan R, Jacobson A. Intra- and intermolecular regulatory interactions in Upf1, the RNA helicase central to nonsense-mediated mRNA decay in yeast. Mol Cell Biol. 2013 Dec; 33(23):4672-84.

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