Two-Hybrid System Techniques

"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity.

MeSH information

Definition | Details | More General Concepts | Related Concepts | More Specific Concepts

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

Descriptor ID	D020798
MeSH Number(s)	E05.393.220.870 E05.601.690.650 E05.601.870
Concept/Terms	Two-Hybrid System Techniques Two-Hybrid System Techniques Technique, Two-Hybrid System Techniques, Two-Hybrid System Two Hybrid System Techniques Two-Hybrid System Technique Two-Hybrid Method Method, Two-Hybrid Methods, Two-Hybrid Two Hybrid Method Two-Hybrid Methods Two-Hybrid System Technics Technic, Two-Hybrid System Technics, Two-Hybrid System Two Hybrid System Technics Two-Hybrid System Technic Two-Hybrid Assay Assay, Two-Hybrid Assays, Two-Hybrid Two Hybrid Assay Two-Hybrid Assays One-Hybrid System Techniques One-Hybrid System Techniques One Hybrid System Techniques One-Hybrid System Technique Technique, One-Hybrid System Techniques, One-Hybrid System One-Hybrid System Technics One Hybrid System Technics One-Hybrid System Technic Technic, One-Hybrid System Technics, One-Hybrid System Reverse One-Hybrid System Techniques Reverse One-Hybrid System Techniques Reverse One Hybrid System Techniques Yeast Two-Hybrid System Techniques Yeast Two-Hybrid System Techniques Yeast Two Hybrid System Techniques Yeast Two-Hybrid System System, Yeast Two-Hybrid Systems, Yeast Two-Hybrid Two-Hybrid System, Yeast Two-Hybrid Systems, Yeast Yeast Two Hybrid System Yeast Two-Hybrid Systems Yeast Two-Hybrid Assay Assay, Yeast Two-Hybrid Assays, Yeast Two-Hybrid Two-Hybrid Assay, Yeast Two-Hybrid Assays, Yeast Yeast Two Hybrid Assay Yeast Two-Hybrid Assays Reverse Two-Hybrid System Techniques Reverse Two-Hybrid System Techniques Reverse Two Hybrid System Techniques Three-Hybrid System Techniques Three-Hybrid System Techniques Technique, Three-Hybrid System Techniques, Three-Hybrid System Three Hybrid System Techniques Three-Hybrid System Technique Tri-Hybrid System Techniques Technique, Tri-Hybrid System Techniques, Tri-Hybrid System Tri Hybrid System Techniques Tri-Hybrid System Technique Three-Hybrid System Technics Technic, Three-Hybrid System Technics, Three-Hybrid System Three Hybrid System Technics Three-Hybrid System Technic Yeast One-Hybrid System Techniques Yeast One-Hybrid System Techniques Yeast One Hybrid System Techniques Yeast Three-Hybrid System Techniques Yeast Three-Hybrid System Techniques Yeast Three Hybrid System Techniques Yeast Three-Hybrid System System, Yeast Three-Hybrid Systems, Yeast Three-Hybrid Three-Hybrid System, Yeast Three-Hybrid Systems, Yeast Yeast Three Hybrid System Yeast Three-Hybrid Systems Yeast Three-Hybrid Assay Assay, Yeast Three-Hybrid Assays, Yeast Three-Hybrid Three-Hybrid Assay, Yeast Three-Hybrid Assays, Yeast Yeast Three Hybrid Assay Yeast Three-Hybrid Assays n-Hybrid System Techniques n-Hybrid System Techniques System Technique, n-Hybrid System Techniques, n-Hybrid Technique, n-Hybrid System Techniques, n-Hybrid System n Hybrid System Techniques n-Hybrid System Technique Reverse Three-Hybrid System Techniques Reverse Three-Hybrid System Techniques Reverse Three Hybrid System Techniques

Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".

Analytical, Diagnostic and Therapeutic Techniques and Equipment [E]
Investigative Techniques [E05]
Genetic Techniques [E05.393]
Cloning, Molecular [E05.393.220]
Two-Hybrid System Techniques [E05.393.220.870]
Molecular Probe Techniques [E05.601]
Protein Interaction Mapping [E05.601.690]
Two-Hybrid System Techniques [E05.601.690.650]
Two-Hybrid System Techniques [E05.601.870]

Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".

publications

Timeline | Most Recent

This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications.

Bar chart showing 8 publications over 7 distinct years, with a maximum of 2 publications in 2008

To see the data from this visualization as text, click here.

Year	Minor Topic	Total
2001	1	1
2004	1	1
2008	2	2
2009	1	1
2010	1	1
2015	1	1
2019	1	1

Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.

Wang LW, Nandadasa S, Annis DS, Dubail J, Mosher DF, Willard BB, Apte SS. A disintegrin-like and metalloproteinase domain with thrombospondin type 1 motif 9 (ADAMTS9) regulates fibronectin fibrillogenesis and turnover. J Biol Chem. 2019 06 21; 294(25):9924-9936.

View in: PubMed
Spinazzola JM, Smith TC, Liu M, Luna EJ, Barton ER. Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle. Hum Mol Genet. 2015 May 01; 24(9):2470-81.

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de Souza EE, Hehnly H, Perez AM, Meirelles GV, Smetana JH, Doxsey S, Kobarg J. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle. 2015; 14(4):656-67.

View in: PubMed
de Souza EE, Meirelles GV, Godoy BB, Perez AM, Smetana JH, Doxsey SJ, McComb ME, Costello CE, Whelan SA, Kobarg J. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res. 2014 Sep 05; 13(9):4074-90.

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Nagarajan A, Dogra SK, Liu AY, Green MR, Wajapeyee N. PEA15 regulates the DNA damage-induced cell cycle checkpoint and oncogene-directed transformation. Mol Cell Biol. 2014 Jun; 34(12):2264-82.

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Reece-Hoyes JS, Marian Walhout AJ. Yeast one-hybrid assays: a historical and technical perspective. Methods. 2012 Aug; 57(4):441-7.

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Min B, Kim MK, Zhang JW, Kim J, Chung KC, Oh BC, Stein GS, Lee YH, van Wijnen AJ, Bae SC. Identification of RUNX3 as a component of the MST/Hpo signaling pathway. J Cell Physiol. 2012 Feb; 227(2):839-49.

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Reece-Hoyes JS, Barutcu AR, McCord RP, Jeong JS, Jiang L, MacWilliams A, Yang X, Salehi-Ashtiani K, Hill DE, Blackshaw S, Zhu H, Dekker J, Walhout AJ. Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nat Methods. 2011 Oct 30; 8(12):1050-2.

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Reece-Hoyes JS, Diallo A, Lajoie B, Kent A, Shrestha S, Kadreppa S, Pesyna C, Dekker J, Myers CL, Walhout AJ. Enhanced yeast one-hybrid assays for high-throughput gene-centered regulatory network mapping. Nat Methods. 2011 Oct 30; 8(12):1059-64.

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Zhu LJ, Christensen RG, Kazemian M, Hull CJ, Enuameh MS, Basciotta MD, Brasefield JA, Zhu C, Asriyan Y, Lapointe DS, Sinha S, Wolfe SA, Brodsky MH. FlyFactorSurvey: a database of Drosophila transcription factor binding specificities determined using the bacterial one-hybrid system. Nucleic Acids Res. 2011 Jan; 39(Database issue):D111-7.

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