 Two-Hybrid System Techniques
"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
| Descriptor ID |
D020798
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| MeSH Number(s) |
E05.393.220.870 E05.393.525.870 E05.601.690.650
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| Concept/Terms |
Two-Hybrid System Techniques- Two-Hybrid System Techniques
- Technique, Two-Hybrid System
- Techniques, Two-Hybrid System
- Two Hybrid System Techniques
- Two-Hybrid System Technique
- Two-Hybrid System Technics
- Technic, Two-Hybrid System
- Technics, Two-Hybrid System
- Two Hybrid System Technics
- Two-Hybrid System Technic
- Two-Hybrid Assay
- Assay, Two-Hybrid
- Assays, Two-Hybrid
- Two Hybrid Assay
- Two-Hybrid Assays
- Two-Hybrid Method
- Method, Two-Hybrid
- Methods, Two-Hybrid
- Two Hybrid Method
- Two-Hybrid Methods
One-Hybrid System Techniques- One-Hybrid System Techniques
- One Hybrid System Techniques
- One-Hybrid System Technique
- Technique, One-Hybrid System
- Techniques, One-Hybrid System
- One-Hybrid System Technics
- One Hybrid System Technics
- One-Hybrid System Technic
- Technic, One-Hybrid System
- Technics, One-Hybrid System
Yeast Two-Hybrid System Techniques- Yeast Two-Hybrid System Techniques
- Yeast Two Hybrid System Techniques
- Yeast Two-Hybrid System
- System, Yeast Two-Hybrid
- Systems, Yeast Two-Hybrid
- Two-Hybrid System, Yeast
- Two-Hybrid Systems, Yeast
- Yeast Two Hybrid System
- Yeast Two-Hybrid Systems
- Yeast Two-Hybrid Assay
- Assay, Yeast Two-Hybrid
- Assays, Yeast Two-Hybrid
- Two-Hybrid Assay, Yeast
- Two-Hybrid Assays, Yeast
- Yeast Two Hybrid Assay
- Yeast Two-Hybrid Assays
Three-Hybrid System Techniques- Three-Hybrid System Techniques
- Technique, Three-Hybrid System
- Techniques, Three-Hybrid System
- Three Hybrid System Techniques
- Three-Hybrid System Technique
- Tri-Hybrid System Techniques
- Technique, Tri-Hybrid System
- Techniques, Tri-Hybrid System
- Tri Hybrid System Techniques
- Tri-Hybrid System Technique
- Three-Hybrid System Technics
- Technic, Three-Hybrid System
- Technics, Three-Hybrid System
- Three Hybrid System Technics
- Three-Hybrid System Technic
Yeast Three-Hybrid System Techniques- Yeast Three-Hybrid System Techniques
- Yeast Three Hybrid System Techniques
- Yeast Three-Hybrid System
- System, Yeast Three-Hybrid
- Systems, Yeast Three-Hybrid
- Three-Hybrid System, Yeast
- Three-Hybrid Systems, Yeast
- Yeast Three Hybrid System
- Yeast Three-Hybrid Systems
- Yeast Three-Hybrid Assay
- Assay, Yeast Three-Hybrid
- Assays, Yeast Three-Hybrid
- Three-Hybrid Assay, Yeast
- Three-Hybrid Assays, Yeast
- Yeast Three Hybrid Assay
- Yeast Three-Hybrid Assays
n-Hybrid System Techniques- n-Hybrid System Techniques
- System Technique, n-Hybrid
- System Techniques, n-Hybrid
- Technique, n-Hybrid System
- Techniques, n-Hybrid System
- n Hybrid System Techniques
- n-Hybrid System Technique
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Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".
Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".
This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications.
To see the data from this visualization as text, click here.
| Year | Major Topic | Minor Topic | Total |
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| 1995 | 0 | 1 | 1 | | 2001 | 0 | 3 | 3 | | 2002 | 0 | 4 | 4 | | 2003 | 0 | 5 | 5 | | 2004 | 0 | 7 | 7 | | 2005 | 1 | 6 | 7 | | 2006 | 1 | 5 | 6 | | 2007 | 1 | 3 | 4 | | 2008 | 1 | 5 | 6 | | 2009 | 0 | 3 | 3 | | 2010 | 0 | 6 | 6 | | 2011 | 3 | 1 | 4 | | 2012 | 0 | 1 | 1 | | 2013 | 0 | 2 | 2 | | 2014 | 0 | 2 | 2 | | 2015 | 0 | 3 | 3 |
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Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.
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Hallstrom KN, Srikanth CV, Agbor TA, Dumont CM, Peters KN, Paraoan L, Casanova JE, Boll EJ, McCormick BA. PERP, a host tetraspanning membrane protein, is required for Salmonella-induced inflammation. Cell Microbiol. 2015 Jun; 17(6):843-59.
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Spinazzola JM, Smith TC, Liu M, Luna EJ, Barton ER. Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle. Hum Mol Genet. 2015 May 1; 24(9):2470-81.
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de Souza EE, Hehnly H, Perez AM, Meirelles GV, Smetana JH, Doxsey S, Kobarg J. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle. 2015; 14(4):656-67.
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de Souza EE, Meirelles GV, Godoy BB, Perez AM, Smetana JH, Doxsey SJ, McComb ME, Costello CE, Whelan SA, Kobarg J. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res. 2014 Sep 05; 13(9):4074-90.
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Nagarajan A, Dogra SK, Liu AY, Green MR, Wajapeyee N. PEA15 regulates the DNA damage-induced cell cycle checkpoint and oncogene-directed transformation. Mol Cell Biol. 2014 Jun; 34(12):2264-82.
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He F, Ganesan R, Jacobson A. Intra- and intermolecular regulatory interactions in Upf1, the RNA helicase central to nonsense-mediated mRNA decay in yeast. Mol Cell Biol. 2013 Dec; 33(23):4672-84.
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Wessel SR, Marceau AH, Massoni SC, Zhou R, Ha T, Sandler SJ, Keck JL. PriC-mediated DNA replication restart requires PriC complex formation with the single-stranded DNA-binding protein. J Biol Chem. 2013 Jun 14; 288(24):17569-78.
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Reece-Hoyes JS, Marian Walhout AJ. Yeast one-hybrid assays: a historical and technical perspective. Methods. 2012 Aug; 57(4):441-7.
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Nottingham RM, Pusapati GV, Ganley IG, Barr FA, Lambright DG, Pfeffer SR. RUTBC2 protein, a Rab9A effector and GTPase-activating protein for Rab36. J Biol Chem. 2012 Jun 29; 287(27):22740-8.
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Min B, Kim MK, Zhang JW, Kim J, Chung KC, Oh BC, Stein GS, Lee YH, van Wijnen AJ, Bae SC. Identification of RUNX3 as a component of the MST/Hpo signaling pathway. J Cell Physiol. 2012 Feb; 227(2):839-49.
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