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Dan Wang PhD

TitleAssistant Professor
InstitutionUMass Chan Medical School
DepartmentRNA Therapeutics Institute
AddressUMass Chan Medical School
368 Plantation Street AS6-2011
Worcester MA 01605
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    Other Positions
    InstitutionT.H. Chan School of Medicine
    DepartmentGene Therapy Center

    InstitutionT.H. Chan School of Medicine
    DepartmentNeuroNexus Institute

    InstitutionT.H. Chan School of Medicine
    DepartmentRNA Therapeutics Institute

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentInterdisciplinary Graduate Program

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentMD/PhD Program

    InstitutionMorningside Graduate School of Biomedical Sciences

    InstitutionMorningside Graduate School of Biomedical Sciences
    DepartmentTranslational Science

    Collapse Biography 
    Collapse education and training
    Fudan University, Shanghai, 31, ChinaBSLife Sciences
    University of Alabama, Birmingham, Birmingham, AL, United StatesPHDGenetics & Genomic Sciences

    Collapse Overview 
    Collapse overview

    Our research centers around gene therapy using adeno-associated virus (AAV) vectors. We carry out translational gene therapy studies targeting several neurological and metabolic diseases. In addition to targeting specific diseases, we develop therapeutic platforms based on molecular approaches such as genome editing and RNA-based methods. We also develop platform technologies to facilitate AAV-based gene therapy, such as animal modeling and AAV vector production.

    In 2020, the lab starts accepting graduate students, postdoctoral researchers, and research associates. Send in your application and get in touch today!

    Collapse Rotation Projects

    - AAV gene therapy for genetic diseases

    - Therapeutic genome editing

    - RNA-based approaches to treating genetic diseases

    - Interactions between AAV vector and host cell

    - AAV vector capsid engineering

    Collapse Post Docs

    We are looking for motivated postdoctoral researchers. Send in your application and get in touch today!

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Liang SQ, Navia AW, Ramseier M, Zhou X, Martinez M, Lee C, Zhou C, Wu J, Xie J, Su Q, Wang D, Flotte TR, Anderson DG, Tarantal AF, Shalek AK, Gao G, Xue W. AAV5 Delivery of CRISPR/Cas9 Mediates Genome Editing in the Lungs of Young Rhesus Monkeys. Hum Gene Ther. 2024 Jul 03. PMID: 38767512.
    2. Liu H, Zhang Y, Yip M, Ren L, Liang J, Chen X, Liu N, Du A, Wang J, Chang H, Oh H, Zhou C, Xing R, Xu M, Guo P, Gessler D, Xie J, Tai PWL, Gao G, Wang D. Producing high-quantity and high-quality recombinant adeno-associated virus by low-cis triple transfection. Mol Ther Methods Clin Dev. 2024 Jun 13; 32(2):101230. PMID: 38558570.
    3. Chang H, Du A, Jiang J, Ren L, Liu N, Zhou X, Liang J, Gao G, Wang D. Non-canonical amino acid incorporation into AAV5 capsid enhances lung transduction in mice. Mol Ther Methods Clin Dev. 2023 Dec 14; 31:101129. PMID: 37886602.
    4. Xu L, Wang D, Zhao L, Yang Z, Liu X, Li X, Yuan T, Wang Y, Huang T, Bian N, He Y, Chen X, Tian B, Liu Z, Luo F, Si W, Gao G, Ji W, Niu Y, Wei J. C9orf72 poly(PR) aggregation in nucleus induces ALS/FTD-related neurodegeneration in cynomolgus monkeys. Neurobiol Dis. 2023 08; 184:106197. PMID: 37328037.
      Citations: 1     Fields:    Translation:AnimalsCells
    5. Vansandt LM, Meinsohn MC, Godin P, Nagykery N, Sicher N, Kano M, Kashiwagi A, Chauvin M, Saatcioglu HD, Barnes JL, Miller AG, Thompson AK, Bateman HL, Donelan EM, Gonz?lez R, Newsom J, Gao G, Donahoe PK, Wang D, Swanson WF, P?pin D. Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-M?llerian hormone transgene. Nat Commun. 2023 06 06; 14(1):3140. PMID: 37280258.
      Citations:    Fields:    Translation:Animals
    6. Yang H, Brown RH, Wang D, Strauss KA, Gao G. Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery. JCI Insight. 2023 05 08; 8(9). PMID: 37014712.
      Citations:    Fields:    Translation:HumansAnimals
    7. Arbab M, Matuszek Z, Kray KM, Du A, Newby GA, Blatnik AJ, Raguram A, Richter MF, Zhao KT, Levy JM, Shen MW, Arnold WD, Wang D, Xie J, Gao G, Burghes AHM, Liu DR. Base editing rescue of spinal muscular atrophy in cells and in mice. Science. 2023 04 21; 380(6642):eadg6518. PMID: 36996170.
      Citations: 23     Fields:    Translation:AnimalsCells
    8. Wang D, Xue X, Gunn G, Du M, Siddiqui A, Weetall M, Keeling KM. Ataluren suppresses a premature termination codon in an MPS I-H mouse. J Mol Med (Berl). 2022 08; 100(8):1223-1235. PMID: 35857082.
      Citations: 3     Fields:    Translation:AnimalsCells
    9. Davidson BL, Gao G, Berry-Kravis E, Bradbury AM, B?nnemann C, Buxbaum JD, Corcoran GR, Gray SJ, Gray-Edwards H, Kleiman RJ, Shaywitz AJ, Wang D, Zoghbi HY, Flotte TR, Tauscher-Wisniewski S, Tifft CJ, Sahin M. Gene-based therapeutics for rare genetic neurodevelopmental psychiatric disorders. Mol Ther. 2022 07 06; 30(7):2416-2428. PMID: 35585789.
      Citations: 7     Fields:    Translation:Humans
    10. Wang J, Zhang Y, Mendonca CA, Yukselen O, Muneeruddin K, Ren L, Liang J, Zhou C, Xie J, Li J, Jiang Z, Kucukural A, Shaffer SA, Gao G, Wang D. AAV-delivered suppressor tRNA overcomes a nonsense mutation in mice. Nature. 2022 04; 604(7905):343-348. PMID: 35322228.
      Citations: 25     Fields:    Translation:HumansAnimalsCells
    11. Ibraheim R, Tai PWL, Mir A, Javeed N, Wang J, Rodr?guez TC, Namkung S, Nelson S, Khokhar ES, Mintzer E, Maitland S, Chen Z, Cao Y, Tsagkaraki E, Wolfe SA, Wang D, Pai AA, Xue W, Gao G, Sontheimer EJ. Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo. Nat Commun. 2021 11 01; 12(1):6267. PMID: 34725353.
      Citations: 31     Fields:    Translation:HumansAnimalsCells
    12. Liang SQ, Walkey CJ, Martinez AE, Su Q, Dickinson ME, Wang D, Lagor WR, Heaney JD, Gao G, Xue W. AAV5 delivery of CRISPR-Cas9 supports effective genome editing in mouse lung airway. Mol Ther. 2022 Jan 05; 30(1):238-243. PMID: 34695545.
      Citations: 17     Fields:    Translation:Animals
    13. Wang D. Juggling Safety and Efficacy: Finding Ways to Achieve Both. Hum Gene Ther. 2021 Jun; 32(11-12):538-539. PMID: 34143660.
      Citations: 1     Fields:    
    14. Meinsohn MC, Saatcioglu HD, Wei L, Li Y, Horn H, Chauvin M, Kano M, Nguyen NMP, Nagykery N, Kashiwagi A, Samore WR, Wang D, Oliva E, Gao G, Morris ME, Donahoe PK, P?pin D. Single-cell sequencing reveals suppressive transcriptional programs regulated by MIS/AMH in neonatal ovaries. Proc Natl Acad Sci U S A. 2021 05 18; 118(20). PMID: 33980714.
      Citations: 14     Fields:    Translation:AnimalsCells
    15. Yang H, Brown RH, Wang D, Strauss KA, Gao G. AAV-Mediated Gene Therapy for Glycosphingolipid Biosynthesis Deficiencies. Trends Mol Med. 2021 06; 27(6):520-523. PMID: 33714697.
      Citations: 5     Fields:    Translation:HumansCells
    16. Tran NT, Heiner C, Weber K, Weiand M, Wilmot D, Xie J, Wang D, Brown A, Manokaran S, Su Q, Zapp ML, Gao G, Tai PWL. AAV-Genome Population Sequencing of Vectors Packaging CRISPR Components Reveals Design-Influenced Heterogeneity. Mol Ther Methods Clin Dev. 2020 Sep 11; 18:639-651. PMID: 32775498.
    17. Yang YS, Xie J, Chaugule S, Wang D, Kim JM, Kim J, Tai PWL, Seo SK, Gravallese E, Gao G, Shim JH. Bone-Targeting AAV-Mediated Gene Silencing in Osteoclasts for Osteoporosis Therapy. Mol Ther Methods Clin Dev. 2020 Jun 12; 17:922-935. PMID: 32405514.
    18. Wang D, Zhang F, Gao G. CRISPR-Based Therapeutic Genome Editing: Strategies and In?Vivo Delivery by AAV Vectors. Cell. 2020 04 02; 181(1):136-150. PMID: 32243786.
      Citations: 168     Fields:    Translation:HumansAnimalsCells
    19. Wang D, Niu Y, Ren L, Kang Y, Tai PWL, Si C, Mendonca CA, Ma H, Gao G, Ji W. Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno-Associated Virus. Adv Sci (Weinh). 2019 Nov 06; 6(21):1900440. PMID: 31728271.
      Citations: 7     Fields:    
    20. Yang YS, Xie J, Wang D, Kim JM, Tai PWL, Gravallese E, Gao G, Shim JH. Bone-targeting AAV-mediated silencing of Schnurri-3 prevents bone loss in osteoporosis. Nat Commun. 2019 07 04; 10(1):2958. PMID: 31273195.
      Citations: 48     Fields:    Translation:AnimalsCells
    21. Saatcioglu HD, Kano M, Horn H, Zhang L, Samore W, Nagykery N, Meinsohn MC, Hyun M, Suliman R, Poulo J, Hsu J, Sacha C, Wang D, Gao G, Lage K, Oliva E, Morris Sabatini ME, Donahoe PK, P?pin D. Single-cell sequencing of neonatal uterus reveals an Misr2+ endometrial progenitor indispensable for fertility. Elife. 2019 06 24; 8. PMID: 31232694.
      Citations: 22     Fields:    Translation:AnimalsCells
    22. Xiao Y, Muhuri M, Li S, Qin W, Xu G, Luo L, Li J, Letizia AJ, Wang SK, Chan YK, Wang C, Fuchs SP, Wang D, Su Q, Nahid MA, Church GM, Farzan M, Yang L, Wei Y, Desrosiers RC, Mueller C, Tai PW, Gao G. Circumventing cellular immunity by miR142-mediated regulation sufficiently supports rAAV-delivered OVA expression without activating humoral immunity. JCI Insight. 2019 05 21; 5. PMID: 31112525.
      Citations: 19     Fields:    Translation:AnimalsCells
    23. Wang D, Tai PWL, Gao G. Adeno-associated virus vector as a platform for gene therapy delivery. Nat Rev Drug Discov. 2019 05; 18(5):358-378. PMID: 30710128.
      Citations: 698     Fields:    Translation:HumansAnimalsCells
    24. Gong Y, Berenson A, Laheji F, Gao G, Wang D, Ng C, Volak A, Kok R, Kreouzis V, Dijkstra IM, Kemp S, Maguire CA, Eichler F. Intrathecal Adeno-Associated Viral Vector-Mediated Gene Delivery for Adrenomyeloneuropathy. Hum Gene Ther. 2019 05; 30(5):544-555. PMID: 30358470.
      Citations: 10     Fields:    Translation:HumansAnimalsCells
    25. Wang D, Zhong L, Li M, Li J, Tran K, Ren L, He R, Xie J, Moser RP, Fraser C, Kuchel T, Sena-Esteves M, Flotte TR, Aronin N, Gao G. Adeno-Associated Virus Neutralizing Antibodies in Large Animals and Their Impact on Brain Intraparenchymal Gene Transfer. Mol Ther Methods Clin Dev. 2018 Dec 14; 11:65-72. PMID: 30397628.
    26. Wang D, Li J, Song CQ, Tran K, Mou H, Wu PH, Tai PWL, Mendonca CA, Ren L, Wang BY, Su Q, Gessler DJ, Zamore PD, Xue W, Gao G. Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice. Nat Biotechnol. 2018 10; 36(9):839-842. PMID: 30102296.
      Citations: 24     Fields:    Translation:AnimalsCells
    27. Song CQ, Wang D, Jiang T, O'Connor K, Tang Q, Cai L, Li X, Weng Z, Yin H, Gao G, Mueller C, Flotte TR, Xue W. In Vivo Genome Editing Partially Restores Alpha1-Antitrypsin in a Murine Model of AAT Deficiency. Hum Gene Ther. 2018 08; 29(8):853-860. PMID: 29597895.
      Citations: 30     Fields:    Translation:HumansAnimalsCells
    28. Wang D, Li J, Tran K, Burt DR, Zhong L, Gao G. Slow Infusion of Recombinant Adeno-Associated Viruses into the Mouse Cerebrospinal Fluid Space. Hum Gene Ther Methods. 2018 04; 29(2):75-85. PMID: 29596011.
      Citations: 5     Fields:    Translation:HumansAnimalsCells
    29. Wang D, Li S, Gessler DJ, Xie J, Zhong L, Li J, Tran K, Van Vliet K, Ren L, Su Q, He R, Goetzmann JE, Flotte TR, Agbandje-McKenna M, Gao G. A Rationally Engineered Capsid Variant of AAV9 for Systemic CNS-Directed and Peripheral Tissue-Detargeted Gene Delivery in Neonates. Mol Ther Methods Clin Dev. 2018 Jun 15; 9:234-246. PMID: 29766031.
    30. Wang D, Gao G. Taking a Hint from Structural Biology: To Better Understand AAV Transport across the BBB. Mol Ther. 2018 02 07; 26(2):336-338. PMID: 29398483.
      Citations: 1     Fields:    
    31. Yoon Y, Wang D, Tai PWL, Riley J, Gao G, Rivera-P?rez JA. Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun. 2018 01 29; 9(1):412. PMID: 29379011.
      Citations: 40     Fields:    Translation:HumansAnimalsCells
    32. Li D, Liu C, Yang C, Wang D, Wu D, Qi Y, Su Q, Gao G, Xu Z, Guo Y. Slow Intrathecal Injection of rAAVrh10 Enhances its Transduction of Spinal Cord and Therapeutic Efficacy in a Mutant SOD1 Model of ALS. Neuroscience. 2017 Dec 04; 365:192-205. PMID: 29024785.
      Citations: 13     Fields:    Translation:AnimalsCells
    33. Xie J, Mao Q, Tai PWL, He R, Ai J, Su Q, Zhu Y, Ma H, Li J, Gong S, Wang D, Gao Z, Li M, Zhong L, Zhou H, Gao G. Short DNA Hairpins Compromise Recombinant Adeno-Associated Virus Genome Homogeneity. Mol Ther. 2017 06 07; 25(6):1363-1374. PMID: 28462820.
      Citations: 44     Fields:    Translation:HumansAnimalsCells
    34. Kano M, Sosulski AE, Zhang L, Saatcioglu HD, Wang D, Nagykery N, Sabatini ME, Gao G, Donahoe PK, P?pin D. AMH/MIS as a contraceptive that protects the ovarian reserve during chemotherapy. Proc Natl Acad Sci U S A. 2017 02 28; 114(9):E1688-E1697. PMID: 28137855.
      Citations: 79     Fields:    Translation:AnimalsCells
    35. Murlidharan G, Sakamoto K, Rao L, Corriher T, Wang D, Gao G, Sullivan P, Asokan A. CNS-restricted Transduction and CRISPR/Cas9-mediated Gene Deletion with an Engineered AAV Vector. Mol Ther Nucleic Acids. 2016 Jul 19; 5(7):e338. PMID: 27434683.
    36. Ai J, Wang D, Wei Q, Li H, Gao G. Adeno-associated Virus Serotype Vectors Efficiently Transduce Normal Prostate Tissue and Prostate Cancer Cells. Eur Urol. 2016 Jan; 69(1):179-81. PMID: 26526960.
      Citations: 7     Fields:    Translation:HumansAnimalsCells
    37. P?pin D, Sosulski A, Zhang L, Wang D, Vathipadiekal V, Hendren K, Coletti CM, Yu A, Castro CM, Birrer MJ, Gao G, Donahoe PK. AAV9 delivering a modified human Mullerian inhibiting substance as a gene therapy in patient-derived xenografts of ovarian cancer. Proc Natl Acad Sci U S A. 2015 Aug 11; 112(32):E4418-27. PMID: 26216943.
      Citations: 30     Fields:    Translation:HumansAnimalsCells
    38. Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ, Weng Z, Gao G, Xue W. Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Hum Gene Ther. 2015 Jul; 26(7):432-42. PMID: 26086867.
      Citations: 160     Fields:    Translation:HumansAnimalsCells
    39. Guo Y, Wang D, Qiao T, Yang C, Su Q, Gao G, Xu Z. A Single Injection of Recombinant Adeno-Associated Virus into the Lumbar Cistern Delivers Transgene Expression Throughout the Whole Spinal Cord. Mol Neurobiol. 2016 07; 53(5):3235-3248. PMID: 26050084.
      Citations: 18     Fields:    Translation:AnimalsCells
    40. Wang D, Gao G. State-of-the-art human gene therapy: part II. Gene therapy strategies and clinical applications. Discov Med. 2014 Sep; 18(98):151-61. PMID: 25227756.
      Citations: 36     Fields:    Translation:HumansCells
    41. Wang D, Gao G. State-of-the-art human gene therapy: part I. Gene delivery technologies. Discov Med. 2014 Jul-Aug; 18(97):67-77. PMID: 25091489.
      Citations: 34     Fields:    Translation:Humans
    42. Wang D, Zhong L, Nahid MA, Gao G. The potential of adeno-associated viral vectors for gene delivery to muscle tissue. Expert Opin Drug Deliv. 2014 Mar; 11(3):345-364. PMID: 24386892.
      Citations: 47     Fields:    Translation:HumansAnimalsCells
    43. Keeling KM, Wang D, Dai Y, Murugesan S, Chenna B, Clark J, Belakhov V, Kandasamy J, Velu SE, Baasov T, Bedwell DM. Attenuation of nonsense-mediated mRNA decay enhances in vivo nonsense suppression. PLoS One. 2013; 8(4):e60478. PMID: 23593225.
      Citations: 68     Fields:    Translation:AnimalsCells
    44. Keeling KM, Wang D, Conard SE, Bedwell DM. Suppression of premature termination codons as a therapeutic approach. Crit Rev Biochem Mol Biol. 2012 Sep; 47(5):444-63. PMID: 22672057.
      Citations: 57     Fields:    Translation:HumansAnimalsCells
    45. Wang D, Belakhov V, Kandasamy J, Baasov T, Li SC, Li YT, Bedwell DM, Keeling KM. The designer aminoglycoside NB84 significantly reduces glycosaminoglycan accumulation associated with MPS I-H in the Idua-W392X mouse. Mol Genet Metab. 2012 Jan; 105(1):116-25. PMID: 22056610.
      Citations: 42     Fields:    Translation:AnimalsCells
    46. Yan L, Wang S, Zhuo Y, Wolf RL, Stiefel MF, An J, Ye Y, Zhang Q, Melhem ER, Wang DJ. Unenhanced dynamic MR angiography: high spatial and temporal resolution by using true FISP-based spin tagging with alternating radiofrequency. Radiology. 2010 Jul; 256(1):270-9. PMID: 20574100.
    47. Wang D, Shukla C, Liu X, Schoeb TR, Clarke LA, Bedwell DM, Keeling KM. Characterization of an MPS I-H knock-in mouse that carries a nonsense mutation analogous to the human IDUA-W402X mutation. Mol Genet Metab. 2010 Jan; 99(1):62-71. PMID: 19751987.
      Citations: 38     Fields:    Translation:HumansAnimals
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