Nucleic Acid Denaturation
"Nucleic Acid Denaturation" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
| Descriptor ID |
D009691
|
| MeSH Number(s) |
E05.393.640 G02.111.603 G05.627
|
| Concept/Terms |
Nucleic Acid Denaturation- Nucleic Acid Denaturation
- Acid Denaturation, Nucleic
- Denaturation, Nucleic Acid
- Nucleic Acid Denaturations
|
Below are MeSH descriptors whose meaning is more general than "Nucleic Acid Denaturation".
Below are MeSH descriptors whose meaning is more specific than "Nucleic Acid Denaturation".
This graph shows the total number of publications written about "Nucleic Acid Denaturation" by people in this website by year, and whether "Nucleic Acid Denaturation" was a major or minor topic of these publications.
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| Year | Major Topic | Minor Topic | Total |
|---|
| 2003 | 0 | 1 | 1 |
| 2008 | 0 | 1 | 1 |
| 2010 | 0 | 2 | 2 |
| 2020 | 1 | 0 | 1 |
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Below are the most recent publications written about "Nucleic Acid Denaturation" by people in Profiles.
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Ghanta KS, Mello CC. Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing in Caenorhabditis elegans. Genetics. 2020 11; 216(3):643-650.
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Anzahaee MY, Watts JK, Alla NR, Nicholson AW, Damha MJ. Energetically important C-H???F-C pseudohydrogen bonding in water: evidence and application to rational design of oligonucleotides with high binding affinity. J Am Chem Soc. 2011 Feb 02; 133(4):728-31.
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Watts JK, Mart?n-Pintado N, G?mez-Pinto I, Schwartzentruber J, Portella G, Orozco M, Gonz?lez C, Damha MJ. Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility. Nucleic Acids Res. 2010 Apr; 38(7):2498-511.
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Ghosh RP, Horowitz-Scherer RA, Nikitina T, Gierasch LM, Woodcock CL. Rett syndrome-causing mutations in human MeCP2 result in diverse structural changes that impact folding and DNA interactions. J Biol Chem. 2008 Jul 18; 283(29):20523-34.
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Martin CT, Ujv?ri A, Liu C. Evaluation of fluorescence spectroscopy methods for mapping melted regions of DNA along the transcription pathway. Methods Enzymol. 2003; 371:13-33.
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Du Z, Regier DA, Desrosiers RC. Improved recombinant PCR mutagenesis procedure that uses alkaline-denatured plasmid template. Biotechniques. 1995 Mar; 18(3):376-8.
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Fathi R, Huang Q, Coppola G, Delaney W, Teasdale R, Krieg AM, Cook AF. Oligonucleotides with novel, cationic backbone substituents: aminoethylphosphonates. Nucleic Acids Res. 1994 Dec 11; 22(24):5416-24.
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Specht CA, DiRusso CC, Novotny CP, Ullrich RC. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem. 1982 Jan 01; 119(1):158-63.
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Abelson HT, Penman S. Induction of alkali labile links in cellular DNA by camptothecin. Biochem Biophys Res Commun. 1973 Feb 20; 50(4):1048-54.